Art. -Nr.: HY-124421

5F-203

(Synonyms: NSC-703786)

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5F-203 (NSC-703786) is a cytotoxic molecule that forms DNA adducts and cell cycle arrest. 5F-203 induces aryl hydrocarbon receptor (AhR) signaling and elevates expression of CYP1A1. 5F-203 also increases the levels of reactive oxygen species as well as activates JNK, ERK, and p38.

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Reinheit: 98.04%
CAS. Nr.: 260443-89-8
Formel: C₁₄H₁₁FN₂S
Molecular Weight:258.31

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

5F-203 Chemical Structure

CAS. Nr.: 260443-89-8

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Biologische Aktivität
- Beschreibung
- Cellular Effect
Chemical Information
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Cellular Effect

Cell Line	Type	Value	Description	References
Cancer cell lines	GI₅₀	<0.01 μM Compound: 10h	Growth inhibition against cancer cell line TH-10 Growth inhibition against cancer cell line TH-10	[PMID: 11311068]
HCC 2998	GI₅₀	>100 μM Compound: 2	Antiproliferative activity against human colon cancer cell line HCC2998 Antiproliferative activity against human colon cancer cell line HCC2998	[PMID: 16392802]
HCC 2998	GI₅₀	<0.01 μM Compound: 130	Cytotoxicity against human HCC 2998 cells Cytotoxicity against human HCC 2998 cells	[PMID: 31926469]
HCT-116	GI₅₀	16 μM Compound: 4a	Antiproliferative activity against human HCT116 cell line Antiproliferative activity against human HCT116 cell line	[PMID: 16789754]
HT-29	GI₅₀	63 μM Compound: 4a	Antiproliferative activity against human HT29 cell line Antiproliferative activity against human HT29 cell line	[PMID: 16789754]
IGROV-1	GI₅₀	<0.01 μM Compound: 10h	Growth inhibition against ovarian cancer cell line IGROV1 Growth inhibition against ovarian cancer cell line IGROV1	[PMID: 11311068]
IGROV-1	GI₅₀	<0.01 μM Compound: 130	Cytotoxicity against human IGROV-1 cells Cytotoxicity against human IGROV-1 cells	[PMID: 31926469]
KM12	GI₅₀	>100 μM Compound: 2	Antiproliferative activity against human colon cancer cell line KM12 Antiproliferative activity against human colon cancer cell line KM12	[PMID: 16392802]
MCF7	GI₅₀	<0.01 μM Compound: 10h	Growth inhibition against breast cancer line MCF-7 Growth inhibition against breast cancer line MCF-7	[PMID: 11311068]
MCF7	GI₅₀	<0.0001 μM Compound: 2	Antiproliferative activity against human breast cancer cell line MCF7 Antiproliferative activity against human breast cancer cell line MCF7	[PMID: 16392802]
MCF7	GI₅₀	<0.1 nM Compound: 2	Antiproliferative activity against human breast cancer cell line MCF7 Antiproliferative activity against human breast cancer cell line MCF7	[PMID: 16392802]
MCF7	GI₅₀	<0.0001 μM Compound: 4a	Antiproliferative activity against human MCF7 cell line Antiproliferative activity against human MCF7 cell line	[PMID: 16789754]
MCF7	GI₅₀	<0.1 nM Compound: 4a	Antiproliferative activity against human MCF7 cell line Antiproliferative activity against human MCF7 cell line	[PMID: 16789754]
MCF7	IC₅₀	0.1 nM Compound: 5F-203	Anticancer activity against human MCF7 cells assessed as inhibition of cell growth Anticancer activity against human MCF7 cells assessed as inhibition of cell growth	[PMID: 26994845]
MCF7	GI₅₀	<0.1 μM Compound: 130	Cytotoxicity against human MCF7 cells Cytotoxicity against human MCF7 cells	[PMID: 31926469]
MDA-MB-468	GI₅₀	<0.0001 μM Compound: 2	Antiproliferative activity against human breast cancer cell line MDA468 Antiproliferative activity against human breast cancer cell line MDA468	[PMID: 16392802]
MDA-MB-468	GI₅₀	<0.1 nM Compound: 2	Antiproliferative activity against human breast cancer cell line MDA468 Antiproliferative activity against human breast cancer cell line MDA468	[PMID: 16392802]
MDA-MB-468	GI₅₀	<0.0001 μM Compound: 4a	Antiproliferative activity against human MDA-MB-468 cell line Antiproliferative activity against human MDA-MB-468 cell line	[PMID: 16789754]
MDA-MB-468	GI₅₀	<0.1 nM Compound: 4a	Antiproliferative activity against human MDA-MB-468 cell line Antiproliferative activity against human MDA-MB-468 cell line	[PMID: 16789754]
NCI-H226	GI₅₀	1.3 μM Compound: 10h	Growth inhibition against non-small cell lung cancer cell line NCI-H266 Growth inhibition against non-small cell lung cancer cell line NCI-H266	[PMID: 11311068]
NCI-H226	GI₅₀	<0.01 μM Compound: 130	Cytotoxicity against human NCI-H226 cells Cytotoxicity against human NCI-H226 cells	[PMID: 31926469]
NCI-H460	GI₅₀	0.05 μM Compound: 10h	Growth inhibition against non-small cell lung cancer cell line NCI-H460 Growth inhibition against non-small cell lung cancer cell line NCI-H460	[PMID: 11311068]
NCI-H460	GI₅₀	<0.01 μM Compound: 130	Cytotoxicity against human NCI-H460 cells Cytotoxicity against human NCI-H460 cells	[PMID: 31926469]
OVCAR-4	GI₅₀	<0.01 μM Compound: 10h	Growth inhibition against ovarian cancer cell line OVCAR-4 Growth inhibition against ovarian cancer cell line OVCAR-4	[PMID: 11311068]
OVCAR-4	GI₅₀	<0.1 μM Compound: 130	Cytotoxicity against human OVCAR-4 cells Cytotoxicity against human OVCAR-4 cells	[PMID: 31926469]
OVCAR-5	GI₅₀	<0.01 μM Compound: 10h	Growth inhibition against ovarian cancer cell line OVCAR-5 Growth inhibition against ovarian cancer cell line OVCAR-5	[PMID: 11311068]
OVCAR-5	GI₅₀	<0.1 μM Compound: 130	Cytotoxicity against human OVCAR-5 cells Cytotoxicity against human OVCAR-5 cells	[PMID: 31926469]
T47D	GI₅₀	<0.01 μM Compound: 10h	Growth inhibition against breast cancer line T-47-D Growth inhibition against breast cancer line T-47-D	[PMID: 11311068]

Chemical Information

CAS. Nr. 260443-89-8
Appearance Solid
Molecular Weight 258.31
Formel C₁₄H₁₁FN₂S
Color Off-white to light yellow
SMILES

NC1=CC=C(C2=NC3=CC(F)=CC=C3S2)C=C1C
Synonyms

NSC-703786
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 100 mg/mL (387.13 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.8713 mL

19.3566 mL

38.7132 mL

5 mM

0.7743 mL

3.8713 mL

7.7426 mL

10 mM

0.3871 mL

1.9357 mL

3.8713 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (9.68 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (270 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Hutchinson I, et al. Antitumor benzothiazoles. 16. Synthesis and pharmaceutical properties of antitumor 2-(4-aminophenyl)benzothiazole amino acid prodrugs. J Med Chem. 2002 Jan 31;45(3):744-7. [Content Brief]

[2]. Hose CD, et al. Induction of CYP1A1 in tumor cells by the antitumor agent 2-[4-amino-3-methylphenyl]-5-fluoro-benzothiazole: a potential surrogate marker for patient sensitivity. Mol Cancer Ther. 2003 Dec;2(12):1265-72. [Content Brief]

[3]. Callero MA, et al. Biomarkers of sensitivity to potent and selective antitumor 2-(4-amino-3-methylphenyl)-5-fluorobenzothiazole (5F203) in ovarian cancer. J Cell Biochem. 2013 Oct;114(10):2392-404. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.8713 mL	19.3566 mL	38.7132 mL	96.7829 mL
	5 mM	0.7743 mL	3.8713 mL	7.7426 mL	19.3566 mL
	10 mM	0.3871 mL	1.9357 mL	3.8713 mL	9.6783 mL
	15 mM	0.2581 mL	1.2904 mL	2.5809 mL	6.4522 mL
	20 mM	0.1936 mL	0.9678 mL	1.9357 mL	4.8391 mL
	25 mM	0.1549 mL	0.7743 mL	1.5485 mL	3.8713 mL
	30 mM	0.1290 mL	0.6452 mL	1.2904 mL	3.2261 mL
	40 mM	0.0968 mL	0.4839 mL	0.9678 mL	2.4196 mL
	50 mM	0.0774 mL	0.3871 mL	0.7743 mL	1.9357 mL
	60 mM	0.0645 mL	0.3226 mL	0.6452 mL	1.6130 mL
	80 mM	0.0484 mL	0.2420 mL	0.4839 mL	1.2098 mL
	100 mM	0.0387 mL	0.1936 mL	0.3871 mL	0.9678 mL

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5F-203

Immunology/Inflammation Compound Library

Transcription Factor-Targeted Library

Human Metabolite Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Mass Spectrometry Human Metabolite Library

Biologische Aktivität

Chemical Information

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

5F-203

Immunology/Inflammation Compound Library

Transcription Factor-Targeted Library

Human Metabolite Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Mass Spectrometry Human Metabolite Library

Biologische Aktivität

Chemical Information

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

5F-203 Related Classifications

HY-124421 Related Classifications