Art. -Nr.: HY-N6712

Thiolutin

(Synonyms: Acetopyrrothin)

4 Cited Publications

Kundenbewertung

Based on 4 publication(s) in Google Scholar

Thiolutin (Acetopyrrothin) is a sulfur-containing antibiotic, which is a potent inhibitor of bacterial and yeast RNA polymerases. Thiolutin can be produced by Streptomyces. Thiolutin inhibits AMSH (IC₅₀ = 4 μM) and Rpn11 (IC₅₀ = 0.53 μM). Thiolutin is a dual inhibitor of BRCC36 and the NLRP3 inflammasome, exhibiting anti-inflammatory effects. Thiolutin effectively suppresses the interaction between BRCC36 and HMGCR, leading to the inhibition of HCC growth. Thiolutin attenuates pyroptosis and NLRP3 inflammasome activation. Thiolutin markedly alleviates renal injury and inflammatory process in IgAN. Thiolutin is an anti-angiogenic compound which can ease Doxorubicin-induced cardiotoxicity (DOXIC)^{[1][2][3][4][5]}.

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Reinheit: 99.27%
CAS. Nr.: 87-11-6
Formel: C₈H₈N₂O₂S₂
Molecular Weight:228.29

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Thiolutin Chemical Structure

CAS. Nr.: 87-11-6

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (4)
Lösungsmittel & Löslichkeit
- In Vitro
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target

RNA Polymerase

Alle Antibiotic Isoform-spezifische Produkte anzeigen :

Alle Deubiquitinase Isoform-spezifische Produkte anzeigen :

Alle NOD-like Receptor (NLR) Isoform-spezifische Produkte anzeigen :

Alle DNA/RNA Synthesis Isoform-spezifische Produkte anzeigen :

In Vitro

Thiolutin (0.5-2 μM, 24 h) significantly inhibits the expression of BRCC36 and HMGCR proteins in a dose-dependent manner in HepG2 and Hep3B cells^[1].

Thiolutin (0-24 h) attenuates the interaction between HMGCR and BRCC36 in HepG2 cells and reduces the half-life of the HMGCR protein while increasing its ubiquitination level in both HepG2 and Hep3B cells^[1].

Thiolutin (0.1-10 μM, 48 h) inhibits the proliferation of HepG2 and Hep3B cells dose-dependently^[1].

Thiolutin (0.25-0.5 μM, 14 days) elevates RSL3 (HY-100218A)-induced cell death and RSL3-triggered lipid peroxidation in HepG2 cells^[1].

Thiolutin (25-250 nM) inhibits the LDH release induced by LPS (HY-D1056) and Nigericin (HY-127019)^[1].

Thiolutin (0.5 μM, 14 days) reduces the colony-forming ability of HepG2 cancer cells in the presence of Lovastatin (HY-N0504) (5 μM)^[1].

Thiolutin (50 μM, 0-120 min) induces global transcriptional stress in Neurospora^[2].

Thiolutin (50 μM, 1-4 h) inhibits protein degradation by the proteasome in N.crassa and HeLa cells^[2].

Thiolutin (0.001-100 μM) inhibits AMSH with an IC₅₀ of 4 μM, which is higher than its inhibition of Rpn11 (IC₅₀ = 0.53 μM), and this effect is reversible by Zn(cyclen)²⁺^[2].

Thiolutin (10-50 nM, 30 min) attenuates pyroptosis and NLRP3 inflammasome activation in J774 A.1 murine monocyte-macrophage cells^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[3]

Cell Line:

J774 A.1 murine monocyte-macrophage cells
Concentration:

10, 25, 50 nM
Incubation Time:

30 min
Result:

Increased progressively with higher concentrations.

ELISA Assay^[3]

Cell Line:

J774 A.1 murine monocyte-macrophage cells
Concentration:

10, 25, 50 nM
Incubation Time:

30 min
Result:

Suppressed the levels of IL-1β and IL-18.

Western Blot Analysis^[3]

Cell Line:

J774 A.1 murine monocyte-macrophage cells
Concentration:

10, 25, 50 nM
Incubation Time:

30 min
Result:

Suppressed the levels of IL-1β and caspase-1.

In Vivo

Thiolutin (0.75 mg/kg, i.p., every 3 days for 18 days) significantly decreases the volume and weight of the tumor in the BALB/c or C57 mice subcutaneously injected with 0.1 mL of PBS containing SMMC7721, HepG2, or Heap1-6 cells^[1].

Thiolutin (0.5-5 mg/kg, i.p., every 2 days for 4 weeks) alleviates kidney injury, attenuates renal inflammation and modulates the NLRP3 inflammasome pathway in IgAN mice without hepatotoxic effects^[3].

Thiolutin (1 mg/kg, i.p., once daily for 4 weeks ) improves cardiac function, mitigates cardiac remodeling urged by Doxorubicin (DOX) and restrains the NLRP3 inflammasome activation in DOX‑challenged mice^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

BALB/c or C57 mice (5 weeks) were subcutaneously injected with 0.1 mL of PBS containing SMMC7721, HepG2, or Heap1-6 cells^[1]
Dosage:

0.75 mg/kg
Administration:

i.p. every 3 days for 18 days
Result:

Decreased the volume and weight of the tumor.

Animal Model:

IgAN model mice (8 weeks male BALB/c)^[3]
Dosage:

0.5, 2.5, 5 mg/kg
Administration:

i.p. every 2 days for 4 weeks
Result:

Decreased Serum BUN S-cr levels, Serum IgA levels and ACR in urine, which were markedly elevated in the IgAN-positive group.
Diminished the glomerular hypercellularity and matrix expansion.
Ameliorated mesangial proliferation and extracellular matrix expansion.
Reduced the level of IFN-γ, TNF-α and IL-6.
Showed no necrosis or injury in both the liver and heart sections of mice.
Downregulated the mRNA expression levels of NLRP3, IL-1β, IL-18, and caspase-1

Animal Model:

DOX‑challenged Male C57BL/6 mice(8-10 weeks)^[4]
Dosage:

1 mg/kg
Administration:

i.p. once daily for 4 weeks
Result:

Ameliorated the changes of LVEF and LVFS as well as LVESD and LVEDD.
Increased the survival rate to 80%.
Decreased the levels of cTnT, CRP, and BNP.
Ameliorated the larger heart size.
Ameliorated DOX-induced pathological changes in cardiomyocytes, including misalignment, inflammatory cell infiltration, widened intercellular gaps, and abnormal morphology/size.
Decreased the protein levels of NLRP3, C-caspase-1/Pro-c-caspase, and GSDMD-N/GSDMD.

Chemical Information

CAS. Nr. 87-11-6
Appearance Solid
Molecular Weight 228.29
Formel C₈H₈N₂O₂S₂
Color Light yellow to yellow
SMILES

CC(NC1=C2C(N(C)C1=O)=CSS2)=O
Synonyms

Acetopyrrothin
Structure Classification
- Antibiotics
- Other Antibiotics
Initial Source
- Microorganisms
Streptomyces
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (4)

All WBCell Proliferation/Viability AssayRT-PCRApoptosis AnalysisIF

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Journal Impact Factor

ACS Environ Au

Machine Learning-Assisted Recognition of Environmental Sulfur-Containing Chemicals in Nontargeted Mass Spectrometry Analysis of Inadequate Mass Resolution. [Abstract]

2025 Aug 5;5(6):573-582. PMID: 41277996
Anal Chem

Exposome-Scale Investigation of Cl-/Br-Containing Chemicals Using High-Resolution Mass Spectrometry, Multistage Machine Learning, and Cloud Computing. [Abstract]

2025 Jun 3;97(21):11099-11109. PMID: 40401576
JOR Spine

Phosphorylated heat shock protein 27 improves the bone formation ability of osteoblasts and bone marrow stem cells from patients with adolescent idiopathic scoliosis. [Abstract]

2023 Apr 20;6(3):e1256. PMID: 37780830

Thiolutin purchased from MedChemExpress. Usage Cited in: JOR Spine. 2023 Apr 20;6(3):e1256. [Abstract]

The protein levels of p‐HSP27 and HSP27 in AIS osteoblasts with/without Thiolutin (1 μM).

Thiolutin purchased from MedChemExpress. Usage Cited in: JOR Spine. 2023 Apr 20;6(3):e1256. [Abstract]

The mRNA levels of HSP27, ALPL, SPP1 in AIS osteoblasts with/without Thiolutin (1 μM).
J Toxicol Sci

The protective effect of thiolutin on doxorubicin-induced H9c2 cardiomyocyte injury. [Abstract]

2023;48(8):469-479. PMID: 37532580

Thiolutin purchased from MedChemExpress. Usage Cited in: J Toxicol Sci. 2023;48(8):469-479. [Abstract]

CCK-8 assay was employed to measure the toxicity of Thiolutin (THL) (0, 1, 10, 20, 40, 80, 160, 320 nM) to H9c2 cardiomyocytes.

Thiolutin purchased from MedChemExpress. Usage Cited in: J Toxicol Sci. 2023;48(8):469-479. [Abstract]

Cell apoptosis detected via flow cytometry treated with Thiolutin (THL).

Thiolutin purchased from MedChemExpress. Usage Cited in: J Toxicol Sci. 2023;48(8):469-479. [Abstract]

ROS accumulation detected via IF treated with Thiolutin (THL).

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 6.25 mg/mL (27.38 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

4.3804 mL

21.9020 mL

43.8039 mL

5 mM

0.8761 mL

4.3804 mL

8.7608 mL

10 mM

0.4380 mL

2.1902 mL

4.3804 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (290 KB)
SDS (479 KB)
- English - EN (479 KB)
- Français - FR (479 KB)
- Deutsch - DE (479 KB)
- Norwegian - NO (479 KB)
- Español - ES (479 KB)
- Swedish - SV (479 KB)
- Italian - IT (479 KB)
- Korean - KR (479 KB)
- Portuguese - PT (479 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Wang H, et al. BRCC36 Deubiquitinates HMGCR to Regulate the Interplay Between Ferroptosis and Pyroptosis. Adv Sci (Weinh). 2024 Mar;11(11):e2304263. [Content Brief]

[2]. Lauinger L, et al. Thiolutin is a zinc chelator that inhibits the Rpn11 and other JAMM metalloproteases. Nat Chem Biol. 2017 Jul;13(7):709-714. [Content Brief]

[3]. Zhang Y, et al. Thiolutin, a novel NLRP3 inflammasome inhibitor, mitigates IgA nephropathy in mice. Int Immunopharmacol. 2025 Apr 16;152:114440. [Content Brief]

[4]. Cai W, et al. Thiolutin Alleviates Cardiotoxic Effects of Doxorubicin by Suppressing NLRP3 Inflammasome in the Mouse Model. Cardiovasc Toxicol. 2025 Feb;25(2):182-192. [Content Brief]

[5]. Kebaara BW, Nielsen LE, Nickerson KW, Atkin AL. Determination of mRNA half-lives in Candida albicans using thiolutin as a transcription inhibitor. Genome. 2006 Aug;49(8):894-9. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	4.3804 mL	21.9020 mL	43.8039 mL	109.5098 mL
	5 mM	0.8761 mL	4.3804 mL	8.7608 mL	21.9020 mL
	10 mM	0.4380 mL	2.1902 mL	4.3804 mL	10.9510 mL
	15 mM	0.2920 mL	1.4601 mL	2.9203 mL	7.3007 mL
	20 mM	0.2190 mL	1.0951 mL	2.1902 mL	5.4755 mL
	25 mM	0.1752 mL	0.8761 mL	1.7522 mL	4.3804 mL

Keywords

Thiolutin

4 Cited Publications

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Thiolutin

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Publications Citing Use of MedChemExpress (MCE) Thiolutin

Alle Antibiotic Isoform-spezifische Produkte anzeigen

Alle Deubiquitinase Isoform-spezifische Produkte anzeigen

Alle NOD-like Receptor (NLR) Isoform-spezifische Produkte anzeigen

Alle DNA/RNA Synthesis Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (4)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Thiolutin Related Classifications

HY-N6712 Related Classifications