BLK degrader 1
Based on 1 Customer Validation
BLK degrader 1 (compound 9) is a selective degrader of B-lymphoid tyrosine kinase (BLK). BLK degrader 1 shows anticancer activity in several B-lymphoid cell lines.
For research use only. We do not sell to patients.
- Purity: 98.94%
- CAS No.: 3049215-64-4
- Formula: C32H25F3N6O2
- Molecular Weight:582.58
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
BLK[1]
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| DOHH-2 | GI50 |
0.13 μM
Compound: 9
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Growth inhibition of human DOHH-2 cells measured after 72 hrs by cell titer glo luminescent cell viability assay
Growth inhibition of human DOHH-2 cells measured after 72 hrs by cell titer glo luminescent cell viability assay
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[PMID: 37163946] |
| SUD4 | GI50 |
0.18 μM
Compound: 9
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Growth inhibition of human SU-DHL-4 cells measured after 72 hrs by cell titer glo luminescent cell viability assay
Growth inhibition of human SU-DHL-4 cells measured after 72 hrs by cell titer glo luminescent cell viability assay
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[PMID: 37163946] |
| SU-DHL-6 | GI50 |
0.36 μM
Compound: 9
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Growth inhibition of human SU-DHL-6 cells measured after 72 hrs by cell titer glo luminescent cell viability assay
Growth inhibition of human SU-DHL-6 cells measured after 72 hrs by cell titer glo luminescent cell viability assay
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[PMID: 37163946] |
Chemical Information
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CAS No. 3049215-64-4
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Appearance Solid
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Molecular Weight 582.58
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Formula C32H25F3N6O2
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Color Off-white to light yellow
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SMILES
CC1=CC=C(C=C1C(NC2=CN=C(N=C2)NC3=CC=CC(N)=C3)=O)NC(C4=CC=C(C=C4)C5=CC(C(F)(F)F)=CC=C5)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (171.65 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (4.29 mM); Clear solution; Need ultrasonic
This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (267 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.7165 mL | 8.5825 mL | 17.1650 mL | 42.9126 mL |
| 5 mM | 0.3433 mL | 1.7165 mL | 3.4330 mL | 8.5825 mL | |
| 10 mM | 0.1717 mL | 0.8583 mL | 1.7165 mL | 4.2913 mL | |
| 15 mM | 0.1144 mL | 0.5722 mL | 1.1443 mL | 2.8608 mL | |
| 20 mM | 0.0858 mL | 0.4291 mL | 0.8583 mL | 2.1456 mL | |
| 25 mM | 0.0687 mL | 0.3433 mL | 0.6866 mL | 1.7165 mL | |
| 30 mM | 0.0572 mL | 0.2861 mL | 0.5722 mL | 1.4304 mL | |
| 40 mM | 0.0429 mL | 0.2146 mL | 0.4291 mL | 1.0728 mL | |
| 50 mM | 0.0343 mL | 0.1717 mL | 0.3433 mL | 0.8583 mL | |
| 60 mM | 0.0286 mL | 0.1430 mL | 0.2861 mL | 0.7152 mL | |
| 80 mM | 0.0215 mL | 0.1073 mL | 0.2146 mL | 0.5364 mL | |
| 100 mM | 0.0172 mL | 0.0858 mL | 0.1717 mL | 0.4291 mL |
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