Art. -Nr.: HY-B1014

Acenocoumarol

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Acenocoumarol is an anticoagulant that functions as a Vitamin K antagonist. Acenocoumarol inhibits MAPK/ERK/JNK signaling pathway, reduces the nuclear translocation of NF-κB p65, activates Akt/GSK3β signaling pathway. Acenocoumarol induces apoptosis in cell A549, arrests cell cycle at S phase.

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Reinheit: 99.17%
CAS. Nr.: 152-72-7
Formel: C₁₉H₁₅NO₆
Molecular Weight:353.33

Speicherung:

Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month

Acenocoumarol Chemical Structure

CAS. Nr.: 152-72-7

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Biologische Aktivität
- Beschreibung
- In Vitro
- In Vivo
- Clinical Trial
Chemical Information
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Alle p38 MAPK Isoform-spezifische Produkte anzeigen :

Alle JNK Isoform-spezifische Produkte anzeigen :

Alle ERK Isoform-spezifische Produkte anzeigen :

Alle NF-κB Isoform-spezifische Produkte anzeigen :

Alle Akt Isoform-spezifische Produkte anzeigen :

Alle GSK-3 Isoform-spezifische Produkte anzeigen :

Alle PKA Isoform-spezifische Produkte anzeigen :

In Vitro

Acenocoumarol (30-500 μM, 24 h) inhibits the expression of iNOS and COX-2, inhibits the expression of NO, prostaglandin E2, TNF-α, IL-6 and IL-1β in LPS (HY-D1056)-stimulated RAW 264.7 cell^[1].
Acenocoumarol (10-60 μM, 72 h) reduces the expression of tyrosinase, tyrosinase-related protein (TRP)-1, TRP-2 in αMSH-stimulated B16F10 melanoma cell, blocks melanin synthesis through PKA/JNK inhibition and Akt/GSK3β activation^[2].
Acenocoumarol (0-100 μM, 24 h) exhibits cytotoxicity in cancer cell A549 and IMR-90 with IC₅₀ of 28.75 μM and 74.11 μM^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:

B16F10
Concentration:

10-60 μM
Incubation Time:

22 h
Result:

Inhibited the expression of pPKA, pJNK, increased the expression of TRP-1 p-p38, pAKT and pGSK3β.

Cell Cytotoxicity Assay^[4]

Cell Line:

A549 and IMR-90
Concentration:

0-100 μM
Incubation Time:

24 h
Result:

Inhibited cell viability.

In Vivo

Acenocoumarol (50-150 µg/kg, po, once daily for 14 days) alleviates ischemia/reperfusion-induced acute pancreatitis in Wistar rats models^[3].
Acenocoumarol (0.2 mg/kg, po, once daily for 21 days) inhibits tumor growth, reduces cellular infiltration and inflammation in tumor tissues in A549-Xenograft mouse models^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

ischemia/reperfusion-induced acute pancreatitis in Wistar rats models^[3]
Dosage:

50-150 µg/kg
Administration:

po, once daily for 14 days
Result:

Reduced pancreatic edema, necrosis, and hemorrhages, decreased pancreatic inflammatory infiltration and vacuolization of pancreatic acinar cells.

Animal Model:

A549-Xenograft mouse models^[4]
Dosage:

0.2 mg/kg
Administration:

po, once daily for 21 days
Result:

Inhibited tumor growth with a TGI of 45.3%, reduced cellular infiltration and inflammation in tumor tissues.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS. Nr. 152-72-7
Appearance Solid
Molecular Weight 353.33
Formel C₁₉H₁₅NO₆
Color White to off-white
SMILES

O=C1C(C(C2=CC=C([N+]([O-])=O)C=C2)CC(C)=O)=C(O)C3=CC=CC=C3O1
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 100 mg/mL (283.02 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.8302 mL

14.1511 mL

28.3022 mL

5 mM

0.5660 mL

2.8302 mL

5.6604 mL

10 mM

0.2830 mL

1.4151 mL

2.8302 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (7.08 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (7.08 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (5.89 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (280 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Han HJ, et al., Acenocoumarol Exerts Anti-Inflammatory Activity via the Suppression of NF-κB and MAPK Pathways in RAW 264.7 Cells. Molecules. 2023 Feb 22;28(5):2075. [Content Brief]

[2]. Han H, et al., Acenocoumarol, an Anticoagulant Drug, Prevents Melanogenesis in B16F10 Melanoma Cells. Pharmaceuticals (Basel). 2023 Apr 17;16(4):604. [Content Brief]

[3]. Warzecha Z, et al., Therapeutic Effect of Low Doses of Acenocoumarol in the Course of Ischemia/Reperfusion-Induced Acute Pancreatitis in Rats. Int. J. Mol. Sci.2017, 18, 882. Int J Mol Sci. 2019 Jun 24;20(12):3086. [Content Brief]

[4]. Maity S, et al. Exploring acenocoumarol and silodosin as allosteric EGFR inhibitors for the treatment of non-small cell lung cancer[J]. F1000Research, 2024, 13(1398): 1398.

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.8302 mL	14.1511 mL	28.3022 mL	70.7554 mL
	5 mM	0.5660 mL	2.8302 mL	5.6604 mL	14.1511 mL
	10 mM	0.2830 mL	1.4151 mL	2.8302 mL	7.0755 mL
	15 mM	0.1887 mL	0.9434 mL	1.8868 mL	4.7170 mL
	20 mM	0.1415 mL	0.7076 mL	1.4151 mL	3.5378 mL
	25 mM	0.1132 mL	0.5660 mL	1.1321 mL	2.8302 mL
	30 mM	0.0943 mL	0.4717 mL	0.9434 mL	2.3585 mL
	40 mM	0.0708 mL	0.3538 mL	0.7076 mL	1.7689 mL
	50 mM	0.0566 mL	0.2830 mL	0.5660 mL	1.4151 mL
	60 mM	0.0472 mL	0.2359 mL	0.4717 mL	1.1793 mL
	80 mM	0.0354 mL	0.1769 mL	0.3538 mL	0.8844 mL
	100 mM	0.0283 mL	0.1415 mL	0.2830 mL	0.7076 mL

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Acenocoumarol

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