Referencia número: HY-16566

Kanamycin

(Synonyms: Kanamycin A)

33 Cited Publications

Revisión del cliente

Based on 33 publication(s) in Google Scholar

Kanamycin (Kanamycin A) is an orally active antibacterial (gram-negative/positive bacteria) agent, inhibits translocation and causes misencoding by binding to the 70 S ribosomal subunit. Kanamycin shows good inhibitory activity to both M. tuberculosis (sensitive and drug-resistant ) and K. pneumonia, which can be used in studies of tuberculosis and pneumonia.

Para uso exclusivo en investigación. No vendemos a pacientes.

Pureza: 99.97%
No. CAS: 59-01-8
Fòrmula: C₁₈H₃₆N₄O₁₁
Peso molecular:484.50

Almacenamiento:

Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month

Kanamycin Chemical Structure

No. CAS: 59-01-8

Tamaño

Precio

Stock

Cantidad

Amount: USD 0.00

* Seleccione Cantidad antes de agregar artículos.

Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Actividad biológica
- Descripciòn
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
- Ensayo clínico
Chemical Information
Publications (33)
Solvente y solubilidad
- In Vitro
Pureza y Documentación
Referencias
Help & FAQs
Classifications
Bioz

Actividad biológica

Descripciòn

IC₅₀ & Target

Aminoglycoside

Ver todos los productos específicos de isoformas Antibiotic :

Cellular Effect

Cell Line	Type	Value	Description	References
Vero	IC₅₀	>100 μg/mL Compound: KAN	Cytotoxicity against african green monkey Vero cells after 72 hrs by MTS-PMS assay Cytotoxicity against african green monkey Vero cells after 72 hrs by MTS-PMS assay	[PMID: 24087924]

In Vitro

Kanamycin (0.1-100 μg/mL; 2 weeks) exhibits good antibacterial activity (MIC=1-5 μg/mL) to various strains of mycobacteria in vitro^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

mycobacteria H37Rv, H2, H37RvR-PAS, Ravenel, Kirchbergand and BCG.
Concentration:

0.1-100 μg/mL
Incubation Time:

2 weeks
Result:

Showed good antibacterial activity to various strains of mycobacteria (H37Rv, H2, H37RvR-PAS, Ravenel, and BCG) with MICs were 1 μg/mL and 5 μg/mL for strain of Kirchbergand.

In Vivo

Kanamycin (2, 4 mg/kg; s.c.; once daily, 6 times a week for 3 weeks) inhibits growth of bovine tubercle bacilli in lung and spleen of mice^[1].
Kanamycin (1.25, 5 mg/kg; s.c.; single (at 3 h after infection)) inhibits the multiplication of K. pneumonia DT-S in lung, trachea, and blood of mice and in proportion to the dose administration, and also increases the survival rate of mice^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Inbred strain normal mice (14-16 g; bovine tubercle bacilli (Ravenel strain) infected model)^[1].
Dosage:

2, 4 mg/kg
Administration:

Subcutaneous injection; once daily, 6 times a week for 3 weeks.
Result:

Exerted a marked effect to inhibit the multiplication of the tuberculosis in vivo, especially in the lung of mice.

Animal Model:

Slc:ICR male mice (4-week-old; 18-24 g; K. pneumonia DT-S infection model (by the aerosol method))^[2].
Dosage:

1.25, 5 mg/kg
Administration:

Subcutaneous administration; single (at 3 h after infection).
Result:

Suppressed the growth of K. pneumonia DT-S in lung, trachea, and blood in proportion to the dose administration.
Resulted in 90% survival at 6 days after infection (negative control group: all died within 4 days), and cleared the K. pneumonia DT-S from lung, trachea, and blood of mice within 48 h (when dosage at 5 mg/kg).

Ensayo clínico

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

No. CAS 59-01-8
Appearance Solid
Peso molecular 484.50
Fòrmula C₁₈H₃₆N₄O₁₁
Color White to off-white
SMILES

O[C@H]1[C@](O[C@@H]2[C@@H](N)C[C@@H](N)[C@H](O[C@@]3([H])O[C@H](CO)[C@@H](O)[C@H](N)[C@H]3O)[C@H]2O)([H])O[C@H](CN)[C@@H](O)[C@@H]1O
Synonyms

Kanamycin A
Envío

Room temperature in continental US; may vary elsewhere.

Almacenamiento

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (33)

All Microbiological Assay

Sort by:

Journal Impact Factor

Science

Commensal-driven serotonin production modulates in vivo delivery of synthetic and viral vectors. [Abstract]

2026 Mar 19;391(6791):eadu7686. PMID: 41855345
Exploration

A Bioorthogonal and Programmable Bacterial Delivery System for Spatiotemporally Targeted Therapy of Solid Tumors. [Abstract]

2025 Dec 18;5(6):20240396. PMID: 41476656
Nat Microbiol

Mycobacterium tuberculosis-derived linoleic acid increases regulatory T cell function to promote bacterial survival within macrophages. [Abstract]

2025 Nov;10(11):2949-2965. PMID: 41073667
Nucleic Acids Res

PhoP-regulated VirK acts as an accessory factor to maintain virulence in polymyxin-resistant Klebsiella pneumoniae. [Abstract]

2026 Apr 23;54(8):gkag290. PMID: 42033226
Nucleic Acids Res

RNA granule-clustered mitochondrial aminoacyl-tRNA synthetases form multiple complexes with the potential to fine-tune tRNA aminoacylation. [Abstract]

2022 Dec 9;50(22):12951-12968. PMID: 36503967
Adv Sci (Weinh)

Aloperine Suppresses Cancer Progression by Interacting with VPS4A to Inhibit Autophagosome-lysosome Fusion in NSCLC. [Abstract]

2024 Aug;11(31):e2308307. PMID: 39166458
Sci Adv

Imaging of Escherichia coli K5 and glycosaminoglycan precursors via targeted metabolic labeling of capsular polysaccharides in bacteria. [Abstract]

2023 Feb 17;9(7):eade4770. PMID: 36800421
Cell Death Dis

Sodium/glucose cotransporter 1-dependent metabolic alterations induce tamoxifen resistance in breast cancer by promoting macrophage M2 polarization. [Abstract]

2021 May 18;12(6):509. PMID: 34006822
Food Chem

Electrochemical sensor for detecting streptomycin in milk based on label-free aptamer chain and magnetic adsorption. [Abstract]

2023 Mar 1:403:134399. PMID: 36182859
Int J Hydrogen Energy

2018, 43(30), 13821-13828.
Food Res Int

Fructooligosaccharides alleviate early-life antibiotic-exposed food allergy via the Indole-3-propionic acid-AhR-Nrf2 Axis: A multi-omics prospective cohort study. [Abstract]

2026 Jul 31:236:119200. PMID: 42116470
Chin Med

Synergistic potential of Berberine and Wogonin improved adipose inflammation and insulin resistance associated with obesity through HIF-α axis. [Abstract]

2025 Oct 3;20(1):155. PMID: 41039626
J Med Chem

Discovery of 2-Chloro-pyrrolo[2,3- d]pyrimidin-4-one Derivatives as Protein Disulfide Isomerase Inhibitors with a Novel Allosteric-Covalent Binding Mode and Anti-Glioblastoma Activity. [Abstract]

2026 Jan 23. PMID: 41574754
mLife

Identification of a phenyl ester covalent inhibitor of caseinolytic protease and analysis of the ClpP1P2 inhibition in mycobacteria. [Abstract]

2025 Apr 15;4(2):155-168. PMID: 40313980
Microb Biotechnol

Attenuator LRR - a regulatory tool for modulating gene expression in Gram-positive bacteria. [Abstract]

2021 Nov;14(6):2538-2551. PMID: 33720523
Commun Biol

The acetyltransferase CysE modulates virulence and drug resistance of Mycobacterium tuberculosis by interfering with oxidative stress responses. [Abstract]

2025 Oct 6;8(1):1425. PMID: 41053365
Int Immunopharmacol

Early-life antibiotic dysbiosis impairs microbial tryptophan- nicotinic acid metabolism exacerbating food allergy in adulthood. [Abstract]

2025 Jun 26:159:114888. PMID: 40403504
BMC Microbiol

Klebsiella pneumoniae under xylose pressure: the growth adaptation, antimicrobial susceptibility, global proteomics analysis and role of XylA and XylB proteins. [Abstract]

2025 Apr 29;25(1):257. PMID: 40301709
Aquat Toxicol

Antibacterials exert toxic effects on aquatic organisms by inhibiting respiration, inducing oxidative stress, mitochondrial dysfunction and autophagy. [Abstract]

2025 Mar:280:107284. PMID: 39951902
iScience

Impact of endogenous sonosensation on in vivo sonogenetics. [Abstract]

2025 Nov 12;28(12):114030. PMID: 41377656
ACS Infect Dis

Toward a Bactericidal Oral Drug Combination for the Treatment of Mycobacterium abscessus Lung Disease. [Abstract]

2025 Apr 1. PMID: 40168319
J Periodontol

Gingipain and oncostatin M synergistically disrupt kidney tight junctions in periodontitis-associated acute kidney injury. [Abstract]

2024 Sep;95(9):867-879. PMID: 38963713
ACS Infect Dis

Lusutrombopag as a Repurposing Drug in Combination with Aminoglycosides against Vancomycin-Resistant Enterococcus. [Abstract]

2024 Apr 12;10(4):1327-1338. PMID: 38567846
J Virol

Generation of HBV cccDNA using single-stranded M13 phage DNA for authentic minichromosome functionality. [Abstract]

2026 May 14:e0003526. PMID: 42132423
Microbiol Spectr

The adaptive growth and mechanisms of Klebsiella pneumoniae under sucrose and glucose exposure. [Abstract]

2025 Oct 16:e0160325. PMID: 41099518
Microbiol Spectr

Killing of colistin-resistant Aeromonas hydrophila by a synthetic peptide. [Abstract]

2025 Aug 29:e0059025. PMID: 40879403
AMB Express

Repurposing pinaverium bromide against Staphylococcus and its biofilms with new mechanisms. [Abstract]

2024 Dec 24;14(1):141. PMID: 39718732

Kanamycin purchased from MedChemExpress. Usage Cited in: AMB Express. 2024 Dec 24;14(1):141. [Abstract]

Combinational antimicrobial effects between PVB and conventional antibiotics against MRSA ATCC 43,300. TET, Tetracycline. DOX, Doxycycline. E, Erythromycin. AZI, Azithromycin. P, Penicillin. CAZ, Ceftazidime. AMP, Ampicillin. CEZ, Cefazolin. CEF, Cefotaxime. OXA, Oxacillin. CRO, Ceftriaxone. AMK, Amikacin. GEN, Gentamycin, KANA, Kanamycin. TOB, Tobramycin. SPC, Spectinomycin.
J Sep Sci

Oriented Immobilization Coupled With Ligand Fishing Strategy for Rapid Discovery of Direct HMGB1 Inhibitors in Taxus wallichiana var. mairei. [Abstract]

2026 Apr;49(4):e70402. PMID: 41906428
ACS Chem Biol

Investigate Natural Product Indolmycin and the Synthetically Improved Analogue Toward Antimycobacterial Agents. [Abstract]

2022 Jan 21;17(1):39-53. PMID: 34908399
Int J Med Microbiol

Antibacterial and antibiofilm efficacy of repurposing drug hexestrol against methicillin-resistant Staphylococcus aureus. [Abstract]

2023 Mar 28;313(2):151578. PMID: 37001448
Vet Res Commun

Biological characterization of lipoic acid- and heme-dependent Escherichia coli small colony variants isolated from sheep in Xinjiang, China. [Abstract]

2024 Dec;48(6):3859-3872. PMID: 39325108
bioRxiv

2025 May 29.
bioRxiv

2025 January 19.

Solvente y solubilidad

In Vitro:

H₂O : 70 mg/mL (144.48 mM; ultrasonic and warming and heat to 60°C)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.0640 mL

10.3199 mL

20.6398 mL

5 mM

0.4128 mL

2.0640 mL

4.1280 mL

10 mM

0.2064 mL

1.0320 mL

2.0640 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Calculadora de molaridad

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculadora de dilución

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

Pureza y Documentación

Select Batch:

Pureza: 99.98%

Assay: 99.98%

ee.: 99.98%

Ficha de datos (282 KB)
SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instrucciones de manejo (2659 KB)

Referencias

[1]. YANAGISAWA K, et al. Studies on kanamycin, a new antibiotic against tubercle bacilli. I. Effect on virulent tubercle bacilli in vitro and in mice. J Antibiot (Tokyo). 1957 Nov;10(6):233-5. [Content Brief]

[2]. Nishi T, et al. Experimental respiratory tract infection with Klebsiella pneumoniae DT-S in mice: chemotherapy with kanamycin. Antimicrob Agents Chemother. 1980 Mar;17(3):494-505. [Content Brief]

[3]. Misumi M, et al. Interaction of kanamycin and related antibiotics with the large subunit of ribosomes and the inhibition of translocation. Biochem Biophys Res Commun. 1978 Sep 29;84(2):358-65. [Content Brief]

[4]. Misumi M, et al. Mechanism of inhibition of translocation by kanamycin and viomycin: a comparative study with fusidic acid. Biochem Biophys Res Commun. 1980 Jan 29;92(2):647-54. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O	1 mM	2.0640 mL	10.3199 mL	20.6398 mL	51.5996 mL
	5 mM	0.4128 mL	2.0640 mL	4.1280 mL	10.3199 mL
	10 mM	0.2064 mL	1.0320 mL	2.0640 mL	5.1600 mL
	15 mM	0.1376 mL	0.6880 mL	1.3760 mL	3.4400 mL
	20 mM	0.1032 mL	0.5160 mL	1.0320 mL	2.5800 mL
	25 mM	0.0826 mL	0.4128 mL	0.8256 mL	2.0640 mL
	30 mM	0.0688 mL	0.3440 mL	0.6880 mL	1.7200 mL
	40 mM	0.0516 mL	0.2580 mL	0.5160 mL	1.2900 mL
	50 mM	0.0413 mL	0.2064 mL	0.4128 mL	1.0320 mL
	60 mM	0.0344 mL	0.1720 mL	0.3440 mL	0.8600 mL
	80 mM	0.0258 mL	0.1290 mL	0.2580 mL	0.6450 mL
	100 mM	0.0206 mL	0.1032 mL	0.2064 mL	0.5160 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Keywords

Kanamycin

33 Cited Publications

100 mg Obtener un presupuesto

Subscribe to the

MedChemExpress

Newsletter

Nombre del producto:
Referencia número:
Cantidad:
MCE Japan Authorized Agent:

Nombre del producto:
No. CAS:
Species:
Pureza:
Cantidad:

Kanamycin

Antibacterial Compound Library

NMPA-Approved Drug Library

Orally Active Compound Library

FDA Approved & Pharmacopeial Drug Library

Antibiotics Library

Microbial Metabolite Library

Rare Diseases Drug Library

Human Metabolite Library

Heterocyclic Compound Library

Off-patent Drug Library

Anti-tuberculosis Compound Library

Anti-Drug-Resistant Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Drug Repurposing Compound Library Plus

FDA-Approved Drug Library Plus

FDA-Approved Drug Library Mini

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Mass Spectrometry Human Metabolite Library

RNA Binding Bioactive Compound Library

Anti-Infection Compound Library

Immunology/Inflammation Compound Library

FDA-Approved Drug Library

Drug Repurposing Compound Library

Publications Citing Use of MedChemExpress (MCE) Kanamycin

Ver todos los productos específicos de isoformas Antibiotic

Actividad biológica

Chemical Information

Publications (33)

Solvente y solubilidad

Pureza y Documentación

Referencias

Help & FAQs

Complete Stock Solution Preparation Table

Kanamycin

Antibacterial Compound Library

NMPA-Approved Drug Library

Orally Active Compound Library

FDA Approved & Pharmacopeial Drug Library

Antibiotics Library

Microbial Metabolite Library

Rare Diseases Drug Library

Human Metabolite Library

Heterocyclic Compound Library

Off-patent Drug Library

Anti-tuberculosis Compound Library

Anti-Drug-Resistant Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Drug Repurposing Compound Library Plus

FDA-Approved Drug Library Plus

FDA-Approved Drug Library Mini

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Mass Spectrometry Human Metabolite Library

RNA Binding Bioactive Compound Library

Anti-Infection Compound Library

Immunology/Inflammation Compound Library

FDA-Approved Drug Library

Drug Repurposing Compound Library

Publications Citing Use of MedChemExpress (MCE) Kanamycin

Ver todos los productos específicos de isoformas Antibiotic

Actividad biológica

Chemical Information

Publications (33)

Solvente y solubilidad

Pureza y Documentación

Referencias

Help & FAQs

Complete Stock Solution Preparation Table

Kanamycin Related Classifications

HY-16566 Related Classifications