Copper chelator
Copper chelator is a copper chelator with anticancer activity. Copper chelator inhibits mitochondrial complex IV through copper depletion, triggering ROS/RNS stress and activating ERK/JNK/p38/AMPK, while suppressing the NRF2 antioxidant response through Keap1 upregulation. Copper chelator induces paraptosis-like death, endoplasmic reticulum stress, mitochondrial dysfunction, and ATP depletion, and inhibits cancer cell invasion and metastasis by downregulating MMP and FOSL1. Copper chelator chelates Cu (I) to form Cu complexes and releases NO with glutathione. Copper chelator inhibits the proliferation of Cu-sensitive melanoma cells and organoids as well as melanoma tumor growth in mice. Copper chelator can be used for research on melanoma.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- CAS 番号: 3101626-89-2
- 分子式: C32H39N3O9S5
- 分子量:769.99
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保管条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
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生物活性
製品説明
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A-375 | IC50 |
2.90 μM
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Cytotoxicity against human A375 melanoma cells assessed as viability reduction incubated 36 hrs by CCK-8 assay.
Cytotoxicity against human A375 melanoma cells assessed as viability reduction incubated 36 hrs by CCK-8 assay.
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42520734 |
| B16-F10 | IC50 |
10.50 μM
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Cytotoxicity against mouse B16-F10 melanoma cells assessed as viability reduction incubated 36 hrs by CCK-8 assay.
Cytotoxicity against mouse B16-F10 melanoma cells assessed as viability reduction incubated 36 hrs by CCK-8 assay.
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42520734 |
| HK-2 | IC50 |
12.33 μM
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Cytotoxicity against human HK-2 kidney cells assessed as viability reduction incubated 36 hrs by CCK-8 assay.
Cytotoxicity against human HK-2 kidney cells assessed as viability reduction incubated 36 hrs by CCK-8 assay.
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42520734 |
体外実験
Copper chelator (4 μM; 36 h) kills A375 melanoma cells through depletion of Cu+ rather than through extracellular CCCu[1].
Copper chelator (4 μM; 36 h) induces caspase-independent non-apoptotic death in A375 melanoma cells[1].
Copper chelator (4 μM; 12-24 h) activates the MAPK pathway in A375 melanoma cells[1].
Copper chelator (4 μM; 8 h) induces paraptosis in A375 melanoma cells that requires ERK/MAPK activation[1].
Copper chelator (4 μM; 12-48 h) inhibits MMP expression and migration in A375 melanoma cells and melanoma organoids[1].
Copper chelator (4-8 μM; 48 h) reduces the growth and viability of melanoma organoids[1].
Copper chelator (4 μM; 6 h) disrupts mitochondrial integrity in A375 melanoma cells[1].
Copper chelator (4 μM; 12-24 h) induces mitochondrial dysfunction and energy stress in A375 melanoma cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:A375 melanoma cells
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Concentration:4 μM
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Incubation Time:24 h
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Result:Downregulated C-IV expression and dramatically inhibited C-IV enzymatic activity.
Cu2+ supplementation greatly restored C-IV expression and activity.
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Cell Line:A375 melanoma cells
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Concentration:4 μM
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Incubation Time:36 h
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Result:Upregulated Keap1 and downregulated NRF2 and NQO1.
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Cell Line:A375 melanoma cells
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Concentration:4 μM
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Incubation Time:12 h and 24 h
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Result:Significantly upregulated phosphorylated ERK, JNK, and p38.
Cu2+ attenuated ERK and p38 phosphorylation, but p-JNK remained activated.
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Cell Line:A375 melanoma cells
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Concentration:4 μM
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Incubation Time:12 h and 36 h
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Result:Inhibited the migration of A375 melanoma cells.
体内実験
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:C57BL/6 (female, 5-6 weeks old)[1]
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Dosage:15 mg/kg; 30 mg/kg
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Administration:every two days; for 10 days
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Result:Inhibited B16-F10 tumor growth by 64.0% at 15 mg/kg and by 79.2% at 30 mg/kg.
H&E-stained tumor sections showed shrunken, decomposed, and decreased DAPI-labeled blue nuclei.
- Spheroid/Tumor Organoid Invasion Assay
- Ex Vivo Tissue Slice/Explant Invasion Assay
- Patient-Derived Organoid Invasion Assay
- Mitochondrial membrane-potential fluorescent assay
- ROS/oxidative-stress fluorescent staining
- Patient-Derived Orthotopic Xenograft (PDOX)
- 3D Tumor Spheroid Invasion Assay
- Matrigel Transwell/Boyden Chamber Invasion Assay
- Cell Viability Determination by MTT Colorimetric Assay
- Invadopodia/Fluorescent Gelatin Degradation Assay
- Mitochondrial membrane-potential and mitochondrial mass staining
- Patient-Derived Xenograft (PDX)
- Organotypic 3D Invasion Assay
- Cell invasion
- Matrigel/ECM Transwell Invasion Assay
- Subcutaneous Cell-Line-Derived Xenograft
- Orthotopic Cell-Line Xenograft
- 3D Collagen/Hydrogel Matrix Invasion Assay
化学情報
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CAS 番号 3101626-89-2
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分子量 769.99
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分子式 C32H39N3O9S5
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SMILES
CCSCCSCCN(C(C1=CC2=CC=C(C=C2OC1=O)OCCOC3=[N+](ON=C3S(=O)(C4=CC=CC=C4)=O)[O-])=O)CCSCCSCC
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Please store the product under the recommended conditions in the Certificate of Analysis.
純度とドキュメンテーション
参考文献
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
Copper chelator 関連分類
HY-190512 関連分類
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