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製品番号: HY-N21972

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Longilactone

Longilactone is a quassinoid diterpenoid isolated from the roots of Eurycoma longifolia. Longilactone induces apoptosis in MCF-7 cells via the extrinsic caspase pathway, activating the cleavage of caspase-8, caspase-7 and PARP. Longilactone can be used in research related to breast cancer and human lung cancer.

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。

CAS 番号: 129587-09-3
分子式: C₁₉H₂₆O₇
分子量:366.41

保管条件:

Please store the product under the recommended conditions in the Certificate of Analysis.

Longilactone 構造式

CAS 番号: 129587-09-3

価格（税別）

Amount: USD 0.00

* アイテムを追加する前、数量をご選択ください

Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

生物活性
- 製品説明
- IC₅₀ & Target
- Cellular Effect
- 体外実験
化学情報
純度とドキュメンテーション
参考文献
Calculators
Help & FAQs
関連分類
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生物活性

製品説明

IC₅₀ & Target^[1]

Caspase-8

Caspase-7

PARP

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Cellular Effect

Cell Line	Type	Value	Description	References
MCF7	IC₅₀	0.53 μg/mL	Cytotoxic activity against human breast cancer MCF-7 cells assessed via Sulforhodamine B (SRB) assay following 72 hrs incubation. Cytotoxic activity against human breast cancer MCF-7 cells assessed via Sulforhodamine B (SRB) assay following 72 hrs incubation.	article1379247682_Muhamad%20et%20al.pdf
A549	ED₅₀	4.6 μg/mL	Cytotoxicity against human lung cancer A-549 cells. Cytotoxicity against human lung cancer A-549 cells.	14738962
MCF7	ED₅₀	6.1 μg/mL	Cytotoxicity against human breast cancer MCF-7 cells. Cytotoxicity against human breast cancer MCF-7 cells.	14738962

体外実験

Longilactone (0.32-20 µg/mL; 72 h) potently inhibits the viability of human breast cancer MCF-7 cells, with an IC₅₀ of 0.53 µg/mL after 72 h of treatment^[1].
Longilactone (compound 36) exhibits cytotoxic activity against human lung cancer (A-549) cells with an ED₅₀ value of 4.6 μg/mL; it also shows cytotoxic activity against human breast cancer (MCF-7) cells with an ED₅₀ value of 6.1 μg/mL^[3].
Longilactone (5 µg/mL; 12-72 h) induces time-dependent apoptosis in human breast cancer MCF-7 cells^[1].
Longilactone (5 µg/mL; 72 h) induces apoptosis in human breast cancer MCF-7 cells^[1].
Longilactone (5 µg/mL; 0-72 h) activates caspase-8 in a time-dependent manner in human breast cancer MCF-7 cells, but has no effect on the activity of caspase-9^[1].
Longilactone (5 µg/mL; 0-72 h) upregulates the levels of activated caspase-8, activated caspase-7 and cleaved PARP in a time-dependent manner, activates the extrinsic apoptotic pathway in human breast cancer MCF-7 cells, and does not alter the expression of Bax and Bcl-2 or activate caspase-9^[1].
Longilactone exhibits no significant anti-HIV activity in H9 lymphocytes^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay^[1]

Cell Line:

human breast cancer MCF-7 cells
Concentration:

0.32, 0.16, 0.8, 4, 20 µg/mL
Incubation Time:

72 h
Result:

Exerted strong cytotoxic activity on MCF-7 cells, with an IC₅₀ of 0.53 µg/mL.

Apoptosis Analysis^[1]

Cell Line:

human breast cancer MCF-7 cells
Concentration:

5 µg/mL
Incubation Time:

12, 24, 48, 72 h
Result:

Induced typical apoptotic nuclear morphology changes including fragmentation, hypercondensation, and shrinkage after 48 h incubation.
Increased the percentage of apoptotic MCF-7 cells in a time-dependent manner, reaching 74.3% after 72 h.

Western Blot Analysis^[1]

Cell Line:

human breast cancer MCF-7 cells
Concentration:

5 µg/mL
Incubation Time:

12, 24, 48, 72 h
Result:

Increased levels of pro-caspase-8 (55 kDa), active caspase-8 (29 kDa), active caspase-7 (20 kDa), and cleaved poly (ADP-ribose) polymerase (PARP, 89 kDa) in a time-dependent manner.
Did not alter basal levels of Bax (20 kDa) and Bcl-2 (26 kDa), and active caspase-9 was not detected.

化学情報

CAS 番号 129587-09-3
分子量 366.41
分子式 C₁₉H₂₆O₇
SMILES

C[C@]12[C@@]3([H])[C@@]4([C@@](C(C)=CC([C@H]4O)=O)([H])[C@H]([C@@]1([H])OC([C@@]2([H])[C@H]([C@H]([C@@H]3O)O)C)=O)O)C
Structure Classification
- Others
Initial Source
- Plants
- Simaroubaceae
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件

Please store the product under the recommended conditions in the Certificate of Analysis.

純度とドキュメンテーション

取扱説明書 (2659 KB)

参考文献

[1]. Shamsul Muhamad, et al. Induction of apoptosis in MCF-7 via the Caspase pathway by longilactone from Eurycoma longifolia Jack. Research In Pharmaceutical Biotechnology Vol. 3(1), pp. 1-10, January 2011.ISSN 2141-2324

[2]. Han YM, et al. Simultaneous quantitation of six major quassinoids in Tongkat Ali dietary supplements by liquid chromatography with tandem mass spectrometry. J Sep Sci. 2015 Jul;38(13):2260-6.

[3]. Kuo PC, et al. Cytotoxic and antimalarial constituents from the roots of Eurycoma longifolia. Bioorganic & medicinal chemistry. 2004 Feb 01;12(3):537-44. [Content Brief]

Calculators

モル濃度計算機

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

濃度濃度

体積体積

分子量分子量

希釈計算機

濃度 _(開始) × 体積 _(開始) = 濃度 _(終了) × 体積 _(終了)

濃度 _(開始) 濃度 _(開始)

体積 _(開始) 体積 _(開始)

濃度 _(終了) 濃度 _(終了)

体積 _(終了) 体積 _(終了)

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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MOQ

Minimum order quantity

100 mg

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生物活性
- 製品説明
- IC₅₀ & Target
- 体外実験
化学情報
純度とドキュメンテーション
参考文献

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Longilactone

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