Art. -Nr.: HY-N3138

Ombuoside

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Ombuoside has antioxidant properties, inhibiting ROS production and apoptosis. Ombuoside exerts neuroprotective effects through the ERK-JNK-caspase-3 system. Ombuoside promotes Dopamine biosynthesis through TH and CREB activation. Ombuoside exhibits antimicrobial activity against several Gram-positive and Gram-negative bacteria, as well as Candida albicans

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Reinheit: 99.68%
CAS. Nr.: 20188-85-6
Formel: C₂₉H₃₄O₁₆
Molecular Weight:638.57

Speicherung:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Ombuoside Chemical Structure

CAS. Nr.: 20188-85-6

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Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target^[2]

JNK1

ERK1

ERK2

Caspase 3

Alle ERK Isoform-spezifische Produkte anzeigen :

Alle JNK Isoform-spezifische Produkte anzeigen :

Alle Caspase Isoform-spezifische Produkte anzeigen :

In Vitro

Ombuoside (0-100 μM, 24-26 h) inhibits HEI-OC1 cells growth only treatment with 80 or 100 μM, increases the survival of Cisplatin (HY-17394)-treated HEI-OC1 cells, with the 30 μM dose demonstrating the most protective effect^[1].
Ombuoside (30 μM, 24 h) reduces Cisplatin-induced apoptosis, oxidative stress via the mitochondria-related apoptotic pathway in HEI-OC1 cells^[1].
Ombuoside (30 μM, 24 h) protects cochlear explants from Cisplatin-induced hair cell damage by inhibiting apoptosis^[1].
Ombuoside (1-100 μM, 1-72 h) reduces PC12 cells viability to 80.4% of the control level at 100 µM, increases the cell viability in L-DOPA (HY-N0304)- induced PC12 cells^[2].
Ombuoside (1-10 μM, 0-6 h) decreases phosphorylation of ERK1/2 and JNK1/2, the expression of cleaved-caspase-3, increases SOD activity in L-DOPA- induced PC12 cells^[2].
Ombuoside (1-10 μM, 0-24 h) increases Dopamine (HY-B0451) levels in a time- and dose-dependent manner, increases the phosphorylation of TH (Ser40) and CREB (Ser133) level in PC12 cells^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Apoptosis Analysis^[1]

Cell Line:

HEI-OC1 cells
Concentration:

30 μM + Cisplatin (30 μM)
Incubation Time:

24 h
Result:

Reduced Cisplatin-induced death and apoptosis.
Significantly decreased the expression of Bax, Bak, cytochrome c, caspase-3, increased the expression of Bcl2.

Immunofluorescence^[1]

Cell Line:

HEI-OC1 cells
Concentration:

30 μM + Cisplatin (30 μMl)
Incubation Time:

24 h
Result:

Reduced CellROX green-positive cells and MitoSOX red-positive cells, reduced ROS levels.
Increased MitoTracker Red fluorescent signal, alleviates mitochondrial damage.

Western Blot Analysis^[2][3]

Cell Line:

HEI-OC1 cells
Concentration:

1, 5, and 10 µM
Incubation Time:

0, 0.5, 1, 3, 6 h
Result:

Increased phosphorylated ERK1/2 at 0.5 and 1 h and then returned to the control level at 3 h.
Decreased phosphorylation of ERK1/2 by cotreatments with 1, 5, and 10 µM to 1.28-, 1.17-, and 1.08-folds of the control level.
Decreased phosphorylation of JNK1/2 by cotreatments with 1, 5, and 10 µMto 1.28-, 1.15-, and 1.06-folds of the control level.
Reduced the expression of cleaved-caspase-3 at 1, 5, and 10 µM to 1.20-, 1.09-, and 1.02-folds of the control level.
Increased SOD activity at 1, 5, and 10 µM to 80.0%, 88.0%, and 90.8% of the control level.
Significantly increased the phosphorylation of TH (Ser40) and CREB (Ser133) level.

In Vivo

Ombuoside (30 μM, 24 h) protects zebrafish hair cells from Cisplatin-induced damage^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Zebrafish model^[1]
Dosage:

30 μM + Cisplatin (30 μM)
Administration:

24 h
Result:

Showed a protective effect on the hair cells of zebrafish neuromasts, resulting in a higher number of hair cells and more regularly shaped cells, compared with those in the Cisplatin group.
Decreased the expression of noxal, baxb, and tp53, upregulated bcl2a.

Chemical Information

CAS. Nr. 20188-85-6
Appearance Solid
Molecular Weight 638.57
Formel C₂₉H₃₄O₁₆
Color White to light yellow
SMILES

O=C1C(O[C@@H]([C@@H]([C@H]2O)O)O[C@@H]([C@H]2O)CO[C@H](O[C@H]3C)[C@@H]([C@@H]([C@H]3O)O)O)=C(C(C=C4)=CC(O)=C4OC)OC5=CC(OC)=CC(O)=C15
Structure Classification
- Flavonoids
- Flavonols
- Phenols
- Polyphenols
Initial Source
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 50 mg/mL (78.30 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.5660 mL

7.8300 mL

15.6600 mL

5 mM

0.3132 mL

1.5660 mL

3.1320 mL

10 mM

0.1566 mL

0.7830 mL

1.5660 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 1.25 mg/mL (1.96 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (288 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Wu X, et al. New application of ombuoside in protecting auditory cells from cisplatin-induced ototoxicity via the apoptosis pathway. Heliyon. 2024 Oct 9;10(20):e39166. [Content Brief]

[2]. Davaasambuu U, et al.Ombuoside from Gynostemma pentaphyllum Protects PC12 Cells from L-DOPA-Induced Neurotoxicity. Planta Med. 2018 Sep;84(14):1007-1012. [Content Brief]

[3]. Uchralsaikhan Davaasambuu1, et al. Ameliorative Effects of Ombuoside on Dopamine Biosynthesis in PC12 Cells. Natural Product Sciences 2018;24(2):99-102.

[4]. Amaro-Luis JM, et al. Isolation, identification and antimicrobial activity of ombuoside from Stevia triflora. Ann Pharm Fr. 1997;55(6):262-8. [Content Brief]

[5]. Abreu PM, et al. Antioxidant compounds from Ebenus pinnata. Fitoterapia. 2007 Jan;78(1):32-4. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.5660 mL	7.8300 mL	15.6600 mL	39.1500 mL
	5 mM	0.3132 mL	1.5660 mL	3.1320 mL	7.8300 mL
	10 mM	0.1566 mL	0.7830 mL	1.5660 mL	3.9150 mL
	15 mM	0.1044 mL	0.5220 mL	1.0440 mL	2.6100 mL
	20 mM	0.0783 mL	0.3915 mL	0.7830 mL	1.9575 mL
	25 mM	0.0626 mL	0.3132 mL	0.6264 mL	1.5660 mL
	30 mM	0.0522 mL	0.2610 mL	0.5220 mL	1.3050 mL
	40 mM	0.0391 mL	0.1957 mL	0.3915 mL	0.9787 mL
	50 mM	0.0313 mL	0.1566 mL	0.3132 mL	0.7830 mL
	60 mM	0.0261 mL	0.1305 mL	0.2610 mL	0.6525 mL

Keywords

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Ombuoside

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Immunology/Inflammation Compound Library

Kinase Inhibitor Library

MAPK Compound Library

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NF-κB Signaling Compound Library

Stem Cell Signaling Compound Library

Natural Product Library

Anti-Aging Compound Library

Alle ERK Isoform-spezifische Produkte anzeigen

Alle JNK Isoform-spezifische Produkte anzeigen

Alle Caspase Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Ombuoside Related Classifications

HY-N3138 Related Classifications