Cat. No.: HY-17003

Saquinavir mesylate

(Synonyms: Ro 31-8959/003)

18 Cited Publications

Customer Review

Based on 18 publication(s) in Google Scholar

Saquinavir (Ro 31-8959) mesylate is an orally active HIV protease inhibitor that can be used in the research of AIDS. Saquinavir mesylate also has anti-inflammatory activity and can induce apoptosis of human red blood cells.

For research use only. We do not sell to patients.

Purity: 99.40%
CAS No.: 149845-06-7
Formula: C₃₉H₅₄N₆O₈S
Molecular Weight:766.95

Storage:

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Saquinavir mesylate Chemical Structure

CAS No.: 149845-06-7

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Biological Activity
- Description
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (18)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

All HIV Isoforms :

All Calcium Channel Isoforms :

All NO Synthase Isoforms :

All Interleukin Related Isoforms :

All Toll-like Receptor (TLR) Isoforms :

All p38 MAPK Isoforms :

All JNK Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
MT4	ED₅₀	0.006 μM Compound: Ro-318959 (Saquinavir mesylate)	Concentration that inhibits cellular growth of MT-4 cells acutely infected with HIV-1 RFstrain by 50% Concentration that inhibits cellular growth of MT-4 cells acutely infected with HIV-1 RFstrain by 50%	10.1016/S0960-894X(01)80332-2

In Vitro

Saquinavir (2.5-15 μg/mL; 48 h) mesylate can induce the apoptosis of human red blood cells, and the mechanism involves the generation of ROS and the influx of Ca²⁺^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Saquinavir (250-2500 mg/kg; administered via diet; 1 month) mesylate exhibits anti-HIV activity in the thy/liv-SCID-hu mouse model^[2].
Saquinavir (0.5 mg/kg; twice) mesylate has a protective effect in the mouse model of lung injury induced by hepatic ischemia-reperfusion (I/R)^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Human thymic and liver tissues (hu-thy/liv) were implanted under both kidney capsules in SCID mice (thy/liv-SCID-hu mice)^[2]
Dosage:

250 or 2500 mg/kg
Administration:

Via diet for 1 month
Result:

Significantly reduced the viral load in the implanted hu-thy/liv and spleens of HIV-infected thy/liv-SCID-hu mice in a dose-related manner.

Animal Model:

C57BL/6 mice aged 8-12 weeks old with hepatic I/R injury model^[3]
Dosage:

0.5 mg/kg
Administration:

Pretreated 1 h before ischemia and again at the time of reperfusion
Result:

Markedly attenuated remote lung tissue injury.
Attenuated I/R-induced lung edema, hyperpermeability, and pathological injury.
Decreased levels of circulating and lung tissue inflammatory cytokines, such as IL-6, IL-1β, TNF-α, and iNOS.
Decreased lung tissue expression of HMGB1, TLR-4, and p-P38/JNK, but not p-ERK.

Chemical Information

CAS No. 149845-06-7
Appearance Solid
Molecular Weight 766.95
Formula C₃₉H₅₄N₆O₈S
Color White to off-white
SMILES

CS(=O)(O)=O.O=C(C1=NC2=C(C=CC=C2)C=C1)N[C@@H](CC(N)=O)C(N[C@@H](CC3=CC=CC=C3)[C@H](O)CN4[C@@H](C[C@@]5([H])[C@@](CCCC5)([H])C4)C(NC(C)(C)C)=O)=O
Synonyms

Ro 31-8959/003
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Publications (18)

All Cell Imaging/StainingELISAWB

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Journal Impact Factor

Signal Transduct Target Ther

Selective depletion of tumor-associated SAMHD1 enhances chemotherapeutic efficacy and antitumor immune responses. [Abstract]

2025 Dec 15;10(1):406. PMID: 41392286
Signal Transduct Target Ther

Bardoxolone and bardoxolone methyl, two Nrf2 activators in clinical trials, inhibit SARS-CoV-2 replication and its 3C-like protease. [Abstract]

2021 May 29;6(1):212. PMID: 34052830
Nat Commun

Nuclear retention of unspliced HIV-1 RNA as a reversible post-transcriptional block in latency. [Abstract]

2025 Feb 28;16(1):2078. PMID: 40021667
Nat Commun

Both Boceprevir and GC376 efficaciously inhibit SARS-CoV-2 by targeting its main protease. [Abstract]

2020 Sep 4;11(1):4417. PMID: 32887884
Chem Eng J

2025 Sep 15.

Saquinavir mesylate purchased from MedChemExpress. Usage Cited in: Chem Eng J. 2025 Sep 15.

Saquinavir (SQV) (64 μg/mL) led to an increase in SA-β-gal-positive cells of SiHa and HeLa cells.

Saquinavir mesylate purchased from MedChemExpress. Usage Cited in: Chem Eng J. 2025 Sep 15.

Saquinavir (SQV) (64 μg/mL) upregulated the levels of SASP factors IL-8, TNF-β, and CXCL-10 in SiHa and HeLa cells.
Phytomedicine

Potential detoxification effect of active ingredients in liquorice by upregulating efflux transporter. [Abstract]

2019 Mar 15:56:175-182. PMID: 30668338
J Med Chem

Development of an In Silico Prediction Model for P-glycoprotein Efflux Potential in Brain Capillary Endothelial Cells toward the Prediction of Brain Penetration. [Abstract]

2021 Mar 11;64(5):2725-2738. PMID: 33619967
Biofactors

GRHL2/SENP1/VDR Signaling Axis: A Key Regulator of SUMOylation and Calcitriol Resistance in SHPT. [Abstract]

2026 Mar-Apr;52(2):e70086. PMID: 41760369
Int J Antimicrob Agents

Efficacy of antiretroviral compounds against Toxoplasma gondii in vitro. [Abstract]

2019 Dec;54(6):814-819. PMID: 31479744
Antimicrob Agents Chemother

Discovery of M Protease Inhibitors Encoded by SARS-CoV-2. [Abstract]

2020 Aug 20;64(9):e00872-20. PMID: 32669265
Antiviral Res

HIV protease inhibitor attenuated astrocyte autophagy involvement in inflammation via p38 MAPK pathway. [Abstract]

2022 Dec:208:105463. PMID: 36372295

Saquinavir mesylate purchased from MedChemExpress. Usage Cited in: Antiviral Res. 2022 Dec:208:105463. [Abstract]

Saquinavir (1, 5, 10, 20 µM; 24 h) inhibits the autophagy in U87 cells, and the expression of p62 is increased in a concentrationdependent manner.

Saquinavir mesylate purchased from MedChemExpress. Usage Cited in: Antiviral Res. 2022 Dec:208:105463. [Abstract]

Saquinavir (10 μM; 24 h) had an obvious autophagic inhibition on U87 cells.
J Virol

HIV proviral transcription and infectivity are enhanced by neddylation. [Abstract]

2025 Sep 30:e0096825. PMID: 41025857

Saquinavir mesylate purchased from MedChemExpress. Usage Cited in: J Virol. 2025 Sep 30:e0096825. [Abstract]

Saquinavir (5 µM) effectively blocked processing of Gag polyprotein into ACH2 extracellular p24 whether MLN was present or not.
J Pharm Sci

Risk Assessment Using Cytochrome P450 Time-Dependent Inhibition Assays at Single Time and Concentration in the Early Stage of Drug Discovery. [Abstract]

2017 Sep;106(9):2839-2846. PMID: 28483425
Exp Cell Res

NLRP3 inflammasome inhibition attenuates cisplatin-induced renal fibrosis by decreasing oxidative stress and inflammation. [Abstract]

2019 Oct 1;383(1):111488. PMID: 31276670
Biochem Biophys Res Commun

Repurposing HIV protease inhibitors as senotherapeutic agents in cervical cancer: Dual targeting of CDK1/6-cell cycle arrest and p53/p21/p16 signaling axis. [Abstract]

2025 May 16:771:152040. PMID: 40403681
Northwestern University

2025.
University of Colorado Denver

2024.
bioRxiv

2020 Apr.

Solvent & Solubility

In Vitro:

DMSO : 16.67 mg/mL (21.74 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.3039 mL

6.5193 mL

13.0387 mL

5 mM

0.2608 mL

1.3039 mL

2.6077 mL

10 mM

0.1304 mL

0.6519 mL

1.3039 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2 mg/mL (2.61 mM); Clear solution

This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2 mg/mL (2.61 mM); Clear solution

This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2 mg/mL (2.61 mM); Clear solution

This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (278 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Waibel S, et al. Saquinavir Induced Suicidal Death of Human Erythrocytes. Cell Physiol Biochem. 2015;37(5):1973-82. [Content Brief]

[2]. Pettoello-Mantovani M, et al. Saquinavir-mediated inhibition of human immunodeficiency virus (HIV) infection in SCID mice implanted with human fetal thymus and liver tissue: an in vivo model for evaluating the effect of drug therapy on HIV infection in lymphoid tissues. Antimicrob Agents Chemother. 1997 Sep;41(9):1880-7. [Content Brief]

[3]. Yu Z, et al. Saquinavir Ameliorates Liver Warm Ischemia-Reperfusion-Induced Lung Injury via HMGB-1- and P38/JNK-Mediated TLR-4-Dependent Signaling Pathways. Mediators Inflamm. 2017;2017:7083528. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.3039 mL	6.5193 mL	13.0387 mL	32.5966 mL
	5 mM	0.2608 mL	1.3039 mL	2.6077 mL	6.5193 mL
	10 mM	0.1304 mL	0.6519 mL	1.3039 mL	3.2597 mL
	15 mM	0.0869 mL	0.4346 mL	0.8692 mL	2.1731 mL
	20 mM	0.0652 mL	0.3260 mL	0.6519 mL	1.6298 mL

Keywords

Saquinavir mesylate

18 Cited Publications

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Saquinavir mesylate

Anti-Infection Compound Library

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Publications Citing Use of MedChemExpress (MCE) Saquinavir mesylate

All HIV Isoforms

All Calcium Channel Isoforms

All NO Synthase Isoforms

All Interleukin Related Isoforms

All Toll-like Receptor (TLR) Isoforms

All p38 MAPK Isoforms