Ionomycin calcium
Based on 130 publication(s) in Google Scholar
Ionomycin calcium (SQ23377 calcium) is a potent, selective calcium ionophore and an antibiotic produced by Streptomyces conglobatus. Ionomycin calcium (SQ23377 calcium) is highly specific for divalent cations (Ca>Mg>Sr=Ba). Ionomycin (SQ23377) promotes apoptosis. Ionomycin calcium (SQ23377 calcium) also induces the activation of protein kinase C (PKC).
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- Reinheit: 98.32%
- CAS. Nr.: 56092-82-1
- Formel: C41H70CaO9
- Molecular Weight:747.07
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Speicherung:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Publications Citing Use of MedChemExpress (MCE) Ionomycin calcium
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Alle Calcium Channel Isoform-spezifische Produkte anzeigen
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Biologische Aktivität
Calcium ionophore[1]
Ionomycin is a Calcium ionophore and an antibiotic produced by Streptomyces conglobatus[1].
Addition of 2 μM Ionomycin to LCLC 103H cells causes an instantaneous increase in intracellular Ca2+ concentration from 50 to 180 nM. DNA and protein analysis in Ionomycin-treated cultures revealed DNA fragmentation and PARP cleavage to an 85-kDa fragment typical of caspase-mediated apoptosis. Necrosis could be detected in ~1-5% of the Ionomycin treated cells. Caspase activation in whole cells was followed by monitoring the increase in activity against Ac-DEVD-amc following Ionomycin treatment[2].
Ionomycin-mediated cleavage and exosome release. Following Ionomycin exposure, medium conditioned by SKOV3ip cells had increased amounts of exosomes containing the L1-32 cleavage fragment[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS. Nr. 56092-82-1
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Appearance Solid
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Molecular Weight 747.07
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Formel C41H70CaO9
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Color White to off-white
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SMILES
O=C([O-])CC[C@@H](C)C[C@H](C)C[C@H](C)C(/C=C([O-])/[C@H](C)C[C@H](C)C/C=C/[C@@H](C)[C@@H](O)[C@@H](C)[C@@H](O)C[C@@H]1CC[C@]([C@@H]2O[C@](C)([C@H](O)C)CC2)(C)O1)=O.[Ca+2]
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Synonyms
SQ23377 calcium
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Structure Classification
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Initial Source
Streptomyces conglobatus
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (130)
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Journal Impact Factor
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Most Recent
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Cancer Cell
Taxanes trigger cancer cell killing in vivo by inducing non-canonical T cell cytotoxicity. [Abstract]2023 Jun 12;41(6):1170-1185.e12. PMID: 37311414 -
Cell
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A High-Fiber Plant-Based Diet in Myeloma Precursor Disorders - Results from the NUTRIVENTION Clinical Trial and Preclinical Vk*MYC Model. [Abstract]2025 Dec 4. PMID: 41342739 -
Cell Metab
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Nat Cancer
Palmitoylation of GPX4 via the targetable ZDHHC8 determines ferroptosis sensitivity and antitumor immunity. [Abstract]2025 May;6(5):768-785. PMID: 40108413 -
Cell Res
Nonenzymatic lysine D-lactylation induced by glyoxalase II substrate SLG dampens inflammatory immune responses. [Abstract]2025 Feb;35(2):97-116. PMID: 39757301 -
Cancer Commun (Lond)
Anti-PD-L1/TGF-βR fusion protein (SHR-1701) overcomes disrupted lymphocyte recovery-induced resistance to PD-1/PD-L1 inhibitors in lung cancer. [Abstract]2022 Jan;42(1):17-36. PMID: 34981670 -
Cell Stem Cell
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Cell Mol Immunol
MAIT cell plasticity generates CD4+ MAIT cells that promote HCC progression via metabolic crosstalk with tumor cells. [Abstract]2026 May;23(5):491-504. PMID: 41974917 -
Cell Mol Immunol
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Cell Mol Immunol
2022 Apr;19(4):540-553. PMID: 35194174 -
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Cell Host Microbe
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Cancer Res
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Cancer Res
Androgen Signaling Contributes to Sex Differences in Cancer by Inhibiting NF-κB Activation in T Cells and Suppressing Anti-Tumor Immunity. [Abstract]2023 Mar 15;83(6):906-921. PMID: 36634207
Ionomycin calcium purchased from MedChemExpress. Usage Cited in: Cancer Res. 2023 Mar 15;83(6):906-921. [Abstract]
The expression of IFNγ or TNFα in CD8+ T cells isolated from B16 tumors was analyzed after re-stimulation by PMA, Ionomycin (500 ng/mL), and BFA for 4 hours in vitro.
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Mol Cell
Localization of a TORC1-eIF4F translation complex during CD8+ T cell activation drives divergent cell fate. [Abstract]2022 Jul 7;82(13):2401-2414.e9. PMID: 35597236
Ionomycin calcium purchased from MedChemExpress. Usage Cited in: Mol Cell. 2022 Jul 7;82(13):2401-2414.e9. [Abstract]
Representative images of undivided OT-I CD8+ T cells activated on slides coated with anti-CD3, anti-CD28, and ICAM1 or with PMA plus Ionomycin (1 μM) for 2 h.
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Nat Commun
Microbiome modulation uncouples efficacy and toxicity induced by immune checkpoint blockade in mouse multiple myeloma. [Abstract]2025 Nov 24;16(1):10384. PMID: 41285849 -
Nat Commun
2025 Mar 19;16(1):2728. PMID: 40108208 -
Nat Commun
THADA inhibition in mice protects against type 2 diabetes mellitus by improving pancreatic β-cell function and preserving β-cell mass. [Abstract]2023 Feb 23;14(1):1020. PMID: 36823211 -
Cell Death Differ
CARM1-mediated hypoxanthine-enriched exosomes rewire inosine metabolism and impair CD8+ T cell antitumor function. [Abstract]2026 Jan 24. PMID: 41580530 -
Cell Death Differ
2025 Apr 16. PMID: 40240520 -
Acta Pharm Sin B
Macrophage P2Y6R activation aggravates psoriatic inflammation through IL-27-mediated Th1 responses. [Abstract]2024 Oct;14(10):4360-4377. PMID: 39525587 -
Sci Transl Med
Combination of metabolic intervention and T cell therapy enhances solid tumor immunotherapy. [Abstract]2020 Nov 25;12(571):eaaz6667. PMID: 33239389 -
Adv Sci (Weinh)
KCNJ2 is Required for NLRP3 Inflammasome Activation That Drives Allergic Airway Inflammation and Remodeling. [Abstract]2026 May;13(29):e17666. PMID: 41960844 -
Adv Sci (Weinh)
O-GlcNAcylated TAP1 Impairs Antigen Presentation and Promotes Immune Evasion in Bladder Cancer. [Abstract]2026 Apr 24:e19955. PMID: 42028629 -
Adv Sci (Weinh)
Thermosensory TRPV Heterotetramers Drive Seasonal Polyphenism: Molecular Basis of CcIav/CcNan-PKCα-AKH/AKHR Signaling in Pear Psyllid Morph Transition. [Abstract]2025 Sep 15:e10102. PMID: 40953294
Ionomycin calcium purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Sep 15:e10102. [Abstract]
CcPKCα modulation by Ionomycin (1 μM in 0.1% DMSO)
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Adv Sci (Weinh)
Nanoparticle-Mediated CXCL12-CXCR4 Inhibition Reprograms Macrophages and Suppresses Gastric Carcinoma. [Abstract]2025 Jun 19:e00225. PMID: 40536774 -
Adv Sci (Weinh)
Mitochondria-Targeted Nanoadjuvants Induced Multi-Functional Immune-Microenvironment Remodeling to Sensitize Tumor Radio-Immunotherapy. [Abstract]2024 Jul;11(26):e2400297. PMID: 38704675 -
Adv Sci (Weinh)
2024 May;11(19):e2306378. PMID: 38482749 -
Adv Sci (Weinh)
MCU Upregulation Overactivates Mitophagy by Promoting VDAC1 Dimerization and Ubiquitination in the Hepatotoxicity of Cadmium. [Abstract]2023 Mar;10(7):e2203869. PMID: 36642847
Ionomycin calcium purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2023 Mar;10(7):e2203869. [Abstract]
Ionomycin (10 μM) served as the positive drug quenching mitochondrial calcein fluorescence.
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J Clin Invest
2025 Aug 1;135(15):e191119. PMID: 40759573 -
Theranostics
2025 May 25;15(13):6329-6346. PMID: 40521193 -
Theranostics
Disulfiram with Cu2+ alleviates dextran sulfate sodium-induced ulcerative colitis in mice. [Abstract]2023 May 8;13(9):2879-2895. PMID: 37284442 -
Protein Cell
Drug target inference by mining transcriptional data using a novel graph convolutional network framework. [Abstract]2022 Apr;13(4):281-301. PMID: 34677780
Ionomycin calcium purchased from MedChemExpress. Usage Cited in: Protein Cell. 2022 Apr;13(4):281-301. [Abstract]
Jurkat T cells were treated with different concentration of NFV or CsA for 2 h, following stimulation with PMA (100 nmol/L) and Ionomycin (10 μmol/L) for 24 h. After treatment, cells and culture supernatant were collected and subjected to RT-qPCR.
Ionomycin calcium purchased from MedChemExpress. Usage Cited in: Protein Cell. 2022 Apr;13(4):281-301. [Abstract]
Jurkat T cells were treated with different concentration of NFV or CsA for 2 h, following stimulation with PMA (100 nmol/L) and Ionomycin (10 μmol/L) for 24 h. After treatment, cells and culture supernatant were collected and subjected to ELISA.
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J Nanobiotechnology
A novel spherical GelMA-HAMA hydrogel encapsulating APET×2 polypeptide and CFIm25-targeting sgRNA for immune microenvironment modulation and nucleus pulposus regeneration in intervertebral discs. [Abstract]2024 Sep 12;22(1):556. PMID: 39267105 -
Redox Biol
A novel CPT1A covalent inhibitor modulates fatty acid oxidation and CPT1A-VDAC1 axis with therapeutic potential for colorectal cancer. [Abstract]2023 Dec:68:102959. PMID: 37977042 -
Gut Microbes
Synergistic activity of Enterococcus Faeciu m-induced ferroptosis via expansion of IFN-γ+CD8+ T cell population in advanced hepatocellular carcinoma treated with sorafenib. [Abstract]2024 Jan-Dec;16(1):2410474. PMID: 39353096 -
EBioMedicine
A broad-spectrum multiepitope vaccine against seasonal influenza A and B viruses in mice. [Abstract]2024 Aug 6:106:105269. PMID: 39111250 -
Cell Rep Med
Fungal metabolite-based immunotherapy overcomes tumor-associated macrophage immunosuppression. [Abstract]2026 Jun 16;7(6):102833. PMID: 42173096 -
J Immunother Cancer
Integrated system for screening tumor-specific TCRs, epitopes, and HLA subtypes using single-cell sequencing data. [Abstract]2025 Jul 31;13(7):e012029. PMID: 40744664 -
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CAR T cells secreting NGF-neutralizing scFv enhance efficacy in clear cell renal cell carcinoma by relieving immunosuppression through immunosympathectomy. [Abstract]2024 Dec 9;12(12):e009910. PMID: 39653553 -
Int J Biol Sci
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Cell Death Dis
FTO controls CD8+ T cell survival and effector response by modulating m6A methylation of Fas. [Abstract]2025 Apr 15;16(1):301. PMID: 40234389 -
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Proc Natl Acad Sci U S A
D-mannose facilitates immunotherapy and radiotherapy of triple-negative breast cancer via degradation of PD-L1. [Abstract]2022 Feb 22;119(8):e2114851119. PMID: 35181605 -
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Polysaccharide from Strongylocentrotus nudus eggs regulates intestinal epithelial autophagy through CD36/PI3K-Akt pathway to ameliorate inflammatory bowel disease. [Abstract]2023 Jul 31:244:125373. PMID: 37327932 -
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TRPV4 channel is involved in HSV-2 infection in human vaginal epithelial cells through triggering Ca2+ oscillation. [Abstract]2023 Apr;44(4):811-821. PMID: 36151392 -
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Ellagic acid alleviates ulcerative colitis in a GPR35-dependent manner associated with SDH restoration. [Abstract]2026 Jun:155:158130. PMID: 41936171 -
Phytomedicine
Cucurbitacin B stimulates PD-1 immunotherapy response in malignant breast cancer by covalent targeting MTCH2. [Abstract]2025 Jun 20:145:157017. PMID: 40582210 -
BMC Med
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BMC Med
Neutrophil extracellular trap-induced intermediate monocytes trigger macrophage activation syndrome in adult-onset Still's disease. [Abstract]2023 Dec 20;21(1):507. PMID: 38124139 -
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CLK1 Promotes Myeloid-Derived Suppressor Cell Trafficking and Reprograms the Tumor Microenvironment by Activating Hippo/YAP Signaling in Colorectal Cancer. [Abstract]2026 May 4;14(5):875-891. PMID: 41686262 -
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Cancer Immunol Res
METTL3 Inhibition Restores PD-L1 Expression and CD8+ T-cell Cytotoxic Function in Immunotherapy-Treated Gastric Cancer. [Abstract]2025 Jul 2;13(7):1037-1052. PMID: 40299705 -
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J Transl Med
MPTP mediated Ox-mtDNA release inducing macrophage pyroptosis and exacerbating MCD-induced MASH via promoting the ITPR3/Ca2+/NLRP3 pathway. [Abstract]2025 Nov 14;23(1):1289. PMID: 41239426 -
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Napabucasin-loaded PLGA nanoparticles trigger anti-HCC immune responses by metabolic reprogramming of tumor-associated macrophages. [Abstract]2024 Dec 20;22(1):1125. PMID: 39707412 -
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Lösungsmittel & Löslichkeit
DMSO : 50 mg/mL (66.93 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (3.35 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (3.35 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
-
+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Reinheit & Dokumentation
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Data Sheet (271 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
Verweise
[1]. Liu C,et al. Characterization of ionomycin as a calcium ionophore. J Biol Chem. 1978 Sep 10;253(17):5892-4. [Content Brief]
[2]. Chatila T, et al. Mechanisms of T cell activation by the calcium ionophore ionomycin. J Immunol. 1989 Aug 15;143(4):1283-9. [Content Brief]
[3]. Gil-Parrado S, et al. Ionomycin-activated calpain triggers apoptosis. A probable role for Bcl-2 family members. J Biol Chem. 2002 Jul 26;277(30):27217-26. [Content Brief]
[4]. Stoeck A, et al A role for exosomes in the constitutive and stimulus-induced ectodomain cleavage of L1 and CD44. Biochem J. 2006 Feb 1;393(Pt 3):609-18. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.3386 mL | 6.6928 mL | 13.3856 mL | 33.4641 mL |
| 5 mM | 0.2677 mL | 1.3386 mL | 2.6771 mL | 6.6928 mL | |
| 10 mM | 0.1339 mL | 0.6693 mL | 1.3386 mL | 3.3464 mL | |
| 15 mM | 0.0892 mL | 0.4462 mL | 0.8924 mL | 2.2309 mL | |
| 20 mM | 0.0669 mL | 0.3346 mL | 0.6693 mL | 1.6732 mL | |
| 25 mM | 0.0535 mL | 0.2677 mL | 0.5354 mL | 1.3386 mL | |
| 30 mM | 0.0446 mL | 0.2231 mL | 0.4462 mL | 1.1155 mL | |
| 40 mM | 0.0335 mL | 0.1673 mL | 0.3346 mL | 0.8366 mL | |
| 50 mM | 0.0268 mL | 0.1339 mL | 0.2677 mL | 0.6693 mL | |
| 60 mM | 0.0223 mL | 0.1115 mL | 0.2231 mL | 0.5577 mL |
Ionomycin calcium Related Classifications
HY-13434A Related Classifications
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