SHC 1
SHC 1 is a dual HDAC8/HDAC6 inhibitor (HDAC8: IC50 = 0.036 μM; HDAC6: IC50 = 1.7 μM) that increases SMC3 acetylation levels. SHC 1 inhibits neuroblastoma cell proliferation, colony formation, and migration, induces caspase 3/7 activity, and exhibits antiproliferative activity against neuroblastoma cells superior to that against normal cells. SHC 1 can be used for research on neuroblastoma.
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- Fòrmula: C11H9N3S4
- Peso molecular:311.47
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Almacenamiento:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Actividad biológica
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Chemical Information
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- Pureza y Documentación
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Actividad biológica
Descripciòn
IC50 & Target
[1]|
HDAC8 0.036 μM (IC50) |
hHDAC6 1.7 μM (IC50) |
Caspase-3 |
Caspase-7 |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| IMR-32 | IC50 |
16.55 μM
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Antiproliferative activity against human IMR-32 neuroblastoma cells assessed as viability reduction by MTT assay.
Antiproliferative activity against human IMR-32 neuroblastoma cells assessed as viability reduction by MTT assay.
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42767177 |
| HCT-116 | IC50 |
38.695 μM
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Antiproliferative activity against human HCT-116 colorectal carcinoma cells assessed as viability reduction by MTT assay.
Antiproliferative activity against human HCT-116 colorectal carcinoma cells assessed as viability reduction by MTT assay.
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42767177 |
| MCF7 | IC50 |
46.405 μM
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Antiproliferative activity against human MCF-7 breast adenocarcinoma cells assessed as viability reduction by MTT assay.
Antiproliferative activity against human MCF-7 breast adenocarcinoma cells assessed as viability reduction by MTT assay.
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42767177 |
| HEK293 | IC50 |
41.05 μM
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Cytotoxicity against normal human HEK-293 cells assessed as viability reduction by MTT assay.
Cytotoxicity against normal human HEK-293 cells assessed as viability reduction by MTT assay.
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42767177 |
In Vitro
SHC-1 (5 μM; 9-12 days) and SHC-2 (10 μM; 9-12 days) reduce colony formation of IMR-32 neuroblastoma cells[1].
SHC-1 (5 μM; 48 h) and SHC-2 (10 μM; 48 h) inhibit the migration of IMR-32 neuroblastoma cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:IMR-32 neuroblastoma cells
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Concentration:5 μM
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Incubation Time:48 h
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Result:Successfully stopped IMR-32 cells from migrating.
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Cell Line:IMR-32 neuroblastoma cells
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Concentration:5 μM, 10 μM
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Incubation Time:24 h
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Result:Observed a dose-dependent increase in acetylated SMC3 levels with compounds SHC-1 and SHC-2 relative to total SMC3 levels.
- BrdU Incorporation Assay
- Cell migration
- Somatic Cell Culture
- CFSE Dye Dilution Proliferation Assay
- Dye-dilution cell tracking and proliferation staining
- CCK-8/WST-8 Cell Proliferation Assay
- Protocol for Cell Cycle
- Cell Counting-Based Growth Curve Assay
- Colony Formation (Clonogenic) Assay
- Cell Viability Determination by MTT Colorimetric Assay
- EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
- Ki-67 Immunostaining Proliferation Assay
- PCNA Immunodetection Proliferation Assay
- Protocol for Cell Counting and Cell Density Analysis
- Apoptosis Solutions
- Soft Agar Colony Formation Assay
- MTT Cell Proliferation Assay
Chemical Information
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Peso molecular 311.47
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Fòrmula C11H9N3S4
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SMILES
N#C/N=C(SC)\SCSC1=NC(C=CC=C2)=C2S1
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureza y Documentación
Referencias
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
SHC 1 Related Classifications
HY-189686 Related Classifications
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