GSK3-IN-10
GSK3-IN-10 (Compound 4) is a multi-target inhibitor, that mainly targets GSK3α and GSK3β with IC50 of 1.0 nM and 2.0 nM. GSK3-IN-10 inhibits the activation of β-catenin, promotes the neuronal survival, and exhibits a protective effect against endoplasmic reticulum stress.
For research use only. We do not sell to patients.
- CAS No.: 844441-56-1
- Formula: C17H18F2N4O3
- Molecular Weight:364.35
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
IC50 & Target
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GSK-3α 1.0 nM (IC50) |
GSK-3β 2.0 nM (IC50) |
DYRK1B 47 nM (IC50) |
DYRK1A 170 nM (IC50) |
ICK 520 nM (IC50) |
CLK1 620 nM (IC50) |
CLK4 760 nM (IC50) |
DYRK2 800 nM (IC50) |
CLK2 800 nM (IC50) |
CDKL5 840 nM (IC50) |
JNK3 2900 nM (IC50) |
CDK4 3800 nM (IC50) |
Chemical Information
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CAS No. 844441-56-1
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Molecular Weight 364.35
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Formula C17H18F2N4O3
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SMILES
O=C(C1=NNC=C1NC(C2=C(F)C=CC=C2F)=O)NCC3CCOCC3
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Serum-Free B27/Neurobasal Neuronal Maintenance Culture
Serum-free B27/Neurobasal culture is a defined neuronal maintenance method designed to support dissociated primary neurons while limiting serum-driven glial expansion; the readout is sustained neuronal survival, neurite extension, neuronal marker expression, synapse formation, and, when measured, electrophysiological or calcium activity. B27/Neurobasal was optimized in embryonic rat hippocampal neurons, where B27 supported >60% survival after 4 days above 160 plated cells/mm2 and Neurobasal reduced glial growth to <0. 5% by immunocytochemistry; later studies extended the approach to cortex, striatum, substantia nigra, septum, cerebellum, and dentate gyrus neurons.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
GSK3-IN-10 Related Classifications
HY-172198 Related Classifications
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