Cat. No.: HY-10357

MK-2206 free base

483 Cited Publications

Customer Review

Based on 483 publication(s) in Google Scholar

MK-2206 free base is an orally active pan-AKT inhibitor, with IC₅₀ values of 8 nM, 12 nM and 65 nM against AKT1, AKT2 and AKT3, respectively. MK-2206 free base inhibits the Akt/mTOR signaling pathway and reduces the levels of downstream GSK3β and Mcl-1 via proteasomal degradation. MK-2206 free base induces G1-phase cell cycle arrest, apoptosis, epithelial-mesenchymal transition, fibroblast activation and extracellular matrix deposition. MK-2206 free base causes transient hyperglycemia and hyperinsulinemia in animals. MK-2206 free base can be used in research related to solid tumors, renal fibrosis and hypercholesterolemia.

For research use only. We do not sell to patients.

CAS No.: 1032349-93-1
Formula: C₂₅H₂₁N₅O
Molecular Weight:407.47

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (299 KB)

MK-2206 free base Chemical Structure

CAS No.: 1032349-93-1

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Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (483)
Purity & Documentation
References
Calculators
Help & FAQs
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Biological Activity

Description

IC₅₀ & Target^[1]

Akt1

8 nM (IC₅₀)

Akt2

12 nM (IC₅₀)

Akt3

65 nM (IC₅₀)

All Akt Isoforms :

All mTOR Isoforms :

All GSK-3 Isoforms :

All Bcl-2 Family Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	8.1 μM Compound: MK2206	Antiproliferative activity against human A549 cells after 48 hrs by MTT assay Antiproliferative activity against human A549 cells after 48 hrs by MTT assay	[PMID: 30777660]
AN3-CA	EC₅₀	972 nM Compound: MK-2206	Antiproliferative activity against human AN3-CA cells assessed as reduction in cell viability incubated for 5 days by celltiter-glo assay Antiproliferative activity against human AN3-CA cells assessed as reduction in cell viability incubated for 5 days by celltiter-glo assay	[PMID: 30996949]
AN3-CA	EC₅₀	972 nM Compound: MK-2206	Antiproliferative activity against human AN3-CA cells assessed as reduction of cell growth measured after 5 days by CellTiter-Glo assay Antiproliferative activity against human AN3-CA cells assessed as reduction of cell growth measured after 5 days by CellTiter-Glo assay	[PMID: 31584233]
BT-474	EC₅₀	1682 nM Compound: MK-2206	Antiproliferative activity against human BT-474 cells assessed as reduction in cell viability incubated for 5 days by celltiter-glo assay Antiproliferative activity against human BT-474 cells assessed as reduction in cell viability incubated for 5 days by celltiter-glo assay	[PMID: 30996949]
HCT-116	IC₅₀	7.5 μM Compound: MK-2206	Growth inhibition of human HCT116 cells after 72 hrs by coulter counter method Growth inhibition of human HCT116 cells after 72 hrs by coulter counter method	[PMID: 24900862]
KU-19-19	EC₅₀	7054 nM Compound: MK-2206	Antiproliferative activity against human KU-19-19 cells assessed as reduction in cell viability incubated for 5 days by celltiter-glo assay Antiproliferative activity against human KU-19-19 cells assessed as reduction in cell viability incubated for 5 days by celltiter-glo assay	[PMID: 30996949]
MCF7	EC₅₀	571 nM Compound: MK-2206	Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability incubated for 5 days by celltiter-glo assay Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability incubated for 5 days by celltiter-glo assay	[PMID: 30996949]
NCI-H1666	CC₅₀	58.5 μM Compound: 57; MK2206	Cytotoxicity against human NCI-H1666 cells Cytotoxicity against human NCI-H1666 cells	[PMID: 33539089]
NCI-H358	IC₅₀	<0.1 μM Compound: A2-NH2; MK2206	Antiproliferation activity against human NCI-H358 cells incubated for 3 days in presence of trametinib by MTT assay Antiproliferation activity against human NCI-H358 cells incubated for 3 days in presence of trametinib by MTT assay	[PMID: 38457912]
NCI-H460	IC₅₀	5.4 μM Compound: MK-2206	Growth inhibition of human NCI-H460 cells after 72 hrs by coulter counter method Growth inhibition of human NCI-H460 cells after 72 hrs by coulter counter method	[PMID: 24900862]
SK-MEL-2	IC₅₀	1 μM Compound: MK-2206	Synergistic cytotoxicity against human SK-MEL-2 cells expressing NRAS mutant assessed as cell growth inhibition measured for 48 hrs in presence of LY3214996 by MTT assay Synergistic cytotoxicity against human SK-MEL-2 cells expressing NRAS mutant assessed as cell growth inhibition measured for 48 hrs in presence of LY3214996 by MTT assay	[PMID: 36961300]
SK-MEL-2	IC₅₀	1 μM Compound: MK-2206	Synergistic cytotoxicity against human SK-MEL-2 cells expressing NRAS mutant assessed as cell growth inhibition measured for 48 hrs in presence of MEK-162 by MTT assay Synergistic cytotoxicity against human SK-MEL-2 cells expressing NRAS mutant assessed as cell growth inhibition measured for 48 hrs in presence of MEK-162 by MTT assay	[PMID: 36961300]
T47D	EC₅₀	411 nM Compound: MK-2206	Antiproliferative activity against human T47D cells assessed as reduction of cell growth measured after 5 days by CellTiter-Glo assay Antiproliferative activity against human T47D cells assessed as reduction of cell growth measured after 5 days by CellTiter-Glo assay	[PMID: 31584233]
T47D	EC₅₀	583 nM Compound: MK-2206	Antiproliferative activity against human T47D cells assessed as reduction in cell viability incubated for 5 days by celltiter-glo assay Antiproliferative activity against human T47D cells assessed as reduction in cell viability incubated for 5 days by celltiter-glo assay	[PMID: 30996949]
ZR-75-1	EC₅₀	571 nM Compound: MK-2206	Antiproliferative activity against human ZR-75-1 cells assessed as reduction of cell growth measured after 5 days by CellTiter-Glo assay Antiproliferative activity against human ZR-75-1 cells assessed as reduction of cell growth measured after 5 days by CellTiter-Glo assay	[PMID: 31584233]
ZR-75-1	EC₅₀	63 nM Compound: MK-2206	Antiproliferative activity against human ZR-75-1 cells assessed as reduction in cell viability incubated for 5 days by celltiter-glo assay Antiproliferative activity against human ZR-75-1 cells assessed as reduction in cell viability incubated for 5 days by celltiter-glo assay	[PMID: 30996949]

In Vitro

MK-2206 free base inhibits Akt1 kinase activity in various human cancer cell lines with an IC₅₀ of approximately 20 nM, blocks the downstream signaling pathway of Akt, and exerts potent antiproliferative effects on cancer cell lines with specific PI3K pathway gene defects, while activation of the Ras pathway predicts no response^[1].
MK-2206 free base exerts additive or synergistic antiproliferative and pro-apoptotic sensitizing effects when combined with various chemotherapeutic agents and targeted inhibitors in relevant human cancer cell lines^[1].
MK-2206 (72 h) free base potently inhibits the growth of U937, OCI/AML3, MV-4-11 and MOLM-13 acute myeloid leukemia (AML) cell lines, with IC₅₀ values ranging from 0.6 to 2.5 μM, while it exhibits only extremely low cytotoxicity against normal human peripheral blood mononuclear cells (PBMCs)^[2].
MK-2206 (1-10 μM; 24 h) free base induces dose-dependent G₁ cell cycle arrest in OCI/AML3, MOLM-13 and MV-4-11 AML cell lines^[2].
MK-2206 (1-10 μM; 24 h) free base induces dose-dependent apoptosis in OCI/AML3, MOLM-13 and MV-4-11 acute myeloid leukemia (AML) cell lines, with a significant increase in apoptotic cell populations at higher doses^[2].
MK-2206 (0.1-10 μM; 2-24 h) free base induces apoptosis in MV-4-11 acute myeloid leukemia (AML) cells via caspase-3 and PARP cleavage, downregulates Mcl-1 protein levels in MV-4-11, OCI/AML3 and U937 AML cells in a dose-dependent manner, and inhibits the phosphorylation of Akt at Ser⁴⁷³ and GSK3β at Ser⁹ after 2 to 24 h of treatment, respectively^[2].
MK-2206 (10 μM; 1-4 h) free base induces downregulation of Mcl-1 in MV-4-11 acute myeloid leukemia (AML) cells via a GSK3β-mediated proteasome-dependent mechanism^[2].
Combined administration of MK-2206 (200 nM; 72 h) free base and Cytarabine (HY-13605) synergistically enhances cytotoxicity in MV-4-11, MOLM-13 and OCI/AML3 acute myeloid leukemia (AML) cell lines (ED₅₀ CI value < 1), but exhibits antagonistic effects in U937 AML cells (ED₅₀ CI value = 1.13)^[2].
MK-2206 (0.5-5 μM; 48 h) free base inhibits TGF-β1-induced fibrosis, epithelial-mesenchymal transition (EMT), and activation of the Akt/mTOR signaling pathway in HK-2 cells. The effective concentration is 1 μM, which reduces the mRNA expression of Collagen I and Fibronectin, restores E-cadherin levels, and suppresses the expression of mesenchymal markers and phosphorylated Akt/mTOR proteins^[3].
MK-2206 (0.5-20 μM; 2-24 h) free base upregulates LDLR protein levels in HepG2 cells treated with sterol feeding or sterol starvation. The maximum induction effect is observed in the sterol starvation group treated with 5 μM for 14 h, while that in the sterol feeding group is achieved with 10 μM treatment for 14 h. Moreover, a significant induction effect occurs within 4 h of treatment with 5 μM^[4].
MK-2206 (2.5-5 μM; 2-6 h) free base inhibits the activity of AKT kinase in sterol-fed HepG2 cells. At concentrations ≥2.5 μM, it reduces the phosphorylation levels of AKT and its downstream target PRAS40 within 2 h^[4].
MK-2206 (5 μM; 14 h) free base increases cell-surface LDLR expression and stimulates LDL uptake in HepG2 cells under both sterol-fed and sterol-starved conditions^[4].
MK-2206 (5-12 μM; 2-24 h) free base induces LDLR mRNA expression in sterol-fed HepG2 cells within 2 h through a mechanism that enhances transcription (rather than mRNA stabilization) and is independent of de novo protein synthesis^[4].
MK-2206 (5-10 μM; 14 h) free base upregulates LDLR protein levels in sterol-fed IHH, HeLa, IMH, and Hepac1c7 cells^[4].
MK-2206 (5 μM; 24 h) free base inhibits de novo cholesterol biosynthesis in HepG2 cells under sterol starvation conditions^[4].
MK-2206 (5 μM; 14 h) free base upregulates LDLR protein levels in CHO cells and HMGCR-deficient UT-2 cells, indicating that this effect is independent of HMGCR activity^[4].
MK-2206 (0.5-4 μM; 38 h) free base enhances the LDLR (low-density lipoprotein receptor)-inducing effect of Mevastatin (HY-17408) in sterol-starved HepG2 cells^[4].
MK-2206 (5 μM; 2-24 h) free base upregulates the mRNA expression of PCSK9, HMGCR, SREBP-2 and HMGCS1 in sterol-fed HepG2 cells, exerts only minor effects on ACACA, FASN and SCD1, and has no effect on IDOL^[4].
MK-2206 (2.5-5 μM; 14-24 h) free base stimulates LDLR promoter activity in sterol-fed HepG2 cells in an SRE-1-dependent manner^[4].
MK-2206 (5 μM; 14-24 h) free base upregulates LDLR mRNA levels in sterol-fed HepG2 cells in an SREBP-2-dependent manner^[4].
MK-2206 (5 μM; 2-6 h) free base stimulates proteolytic cleavage of FL-SREBP-2 to generate the active NTF-SREBP-2 in sterol-fed HepG2 cells^[4].
MK-2206 (2.5-10 μM; 14 h) free base upregulates LDLR protein levels in primary adult hepatocytes^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cycle Analysis^[2]

Cell Line:

OCI/AML3, MOLM-13, MV-4-11 human AML cell lines
Concentration:

1, 5 and 10 μM
Incubation Time:

24 h
Result:

Caused a dose-dependent increase in the percentage of cells in the G₁ phase and a corresponding decrease in cells in the S and G₂/M phases across all three cell lines.
Increased G₁ phase cells in OCI/AML3 cells from ~45% (control) to ~75% (10 μM MK-2206).
Increased G₁ phase cells in MOLM-13 cells from ~45% to ~75%.
Increased G₁ phase cells in MV-4-11 cells from ~60% to ~90%.

Apoptosis Analysis^[2]

Cell Line:

OCI/AML3, MOLM-13, MV-4-11 human AML cell lines
Concentration:

1-10 μM
Incubation Time:

24 h
Result:

Caused a dose-dependent increase in the percentage of apoptotic cells in all three cell lines.
Increased apoptotic cells in OCI/AML3 cells from ~4% (control) to ~11.5% (10 μM MK-2206).
Increased apoptotic cells in MOLM-13 cells from ~3% to ~9.5%.
Increased apoptotic cells in MV-4-11 cells from ~0.5% to ~14.5%.

Western Blot Analysis^[2]

Cell Line:

MV-4-11, OCI/AML3, U937 human AML cell lines
Concentration:

0.1, 1, 5 and 10 μM (24 h incubation in MV-4-11 cells); 0.1, 1, 5 and 10 μM (2 h incubation in MV-4-11, OCI/AML3, U937 cells)
Incubation Time:

24 h (MV-4-11 cells); 2 h (MV-4-11, OCI/AML3, U937 cells)
Result:

Induced dose-dependent cleavage of caspase-3 and PARP, and reduced Mcl-1 protein levels to 51% of control at 10 μM in MV-4-11 cells treated for 24 h, with no effect on Bak, Bcl-2, or Bcl-XL levels.
Reduced Akt phosphorylation at Ser473, reduced GSK3β phosphorylation at Ser9, and reduced Mcl-1 protein levels to 35% of control in MV-4-11, 26% in OCI/AML3, and 33% in U937 at 10 μM in cells treated for 2 h, with no effect on total Akt, total GSK3β, Bcl-2, or Bcl-XL levels.

Real Time qPCR^[2]

Cell Line:

MV-4-11 human AML cell line
Concentration:

0.1, 1, 5 and 10 μM
Incubation Time:

24 h
Result:

Had no significant effect on Mcl-1 mRNA transcript levels in MV-4-11 cells at any tested concentration.

Western Blot Analysis^[2]

Cell Line:

MV-4-11 human AML cell line
Concentration:

10 μM MK-2206 (following 30 min pretreatment with 10 μM MG-132 or 20 mM lithium chloride)
Incubation Time:

1 h, 2 h, 4 h
Result:

Pretreatment with MG-132 prevented MK-2206-associated downregulation of Mcl-1, maintaining Mcl-1 levels at near-control values across all incubation times.
Pretreatment with lithium chloride prevented MK-2206-induced Mcl-1 downregulation and maintained GSK3β phosphorylation at Ser9 at levels comparable to control cells.

Cell Viability Assay^[2]

Cell Line:

U937, OCI/AML3, MV-4-11, MOLM-13 human AML cell lines
Concentration:

200 nM MK-2206 (co-administered with varying cytarabine concentrations)
Incubation Time:

72 h
Result:

Significantly reduced the IC₅₀ of cytarabine in MV-4-11, OCI/AML3, and MOLM-13 cells, but not in U937 cells.
Produced ED₅₀ CI values of 0.59 (MV-4-11), 0.37 (MOLM-13), 0.38 (OCI/AML3), and 1.13 (U937), indicating synergistic effects in the first three cell lines and an antagonistic effect in U937 cells.

Western Blot Analysis^[4]

Cell Line:

human hepatoma HepG2 cells (sterol-fed and sterol-starved conditions)
Concentration:

0.5, 1, 2.5, 5, 10 and 20 μM (for 14 h); 5 μM (for time-course analysis)
Incubation Time:

14 h (dose-response); 2-24 h (time-course with 5 μM)
Result:

Induced LDLR protein expression in a dose-responsive manner, with 5 μM and 10 μM exerting maximal effects in sterol-starved and sterol-fed cells, respectively; concentrations above 10 μM mitigated the LDLR-inducing effect.
Significantly increased LDLR levels within 4 h of 5 μM treatment, reaching maximum levels at 14 h (sterol-starved) and 18 h (sterol-fed), followed by a slight decline by 24 h.

Western Blot Analysis^[4]

Cell Line:

sterol-fed human hepatoma HepG2 cells
Concentration:

2.5, 5 μM (for 2 h); 2.5, 5 μM (for 6 h)
Incubation Time:

2 h; 6 h
Result:

Potently inhibited AKT activity, as shown by reduced pAKT and pPRAS40 levels within 2 h of treatment with concentrations as low as 2.5 μM.

Real Time qPCR^[4]

Cell Line:

sterol-fed human hepatoma HepG2 cells
Concentration:

5 μM
Incubation Time:

2-24 h; 12 h (followed by actinomycin D treatment for 2-6 h); 5 h (with 1 h cycloheximide preincubation)
Result:

Induced LDLR mRNA levels in a time-dependent manner, with significant increases observed within 2 h of treatment.
Had no significant stabilizing effect on LDLR mRNA.
Inhibition of protein synthesis with cycloheximide did not reduce MK-2206-mediated induction of LDLR mRNA.

Western Blot Analysis^[4]

Cell Line:

sterol-fed immortalized human hepatocytes (IHH), HeLa, immortalized mouse hepatocytes (IMH), and Hepac1c7 cells
Concentration:

5, 10 μM
Incubation Time:

14 h
Result:

Induced LDLR protein expression in all tested cell lines, with significant increases observed at 5 μM and 10 μM.

Western Blot Analysis^[4]

Cell Line:

Chinese hamster ovary (CHO) cells and UT-2 cells (HMGCR-deficient CHO cells)
Concentration:

5 μM
Incubation Time:

14 h
Result:

Induced LDLR protein expression in both CHO and UT-2 cells, with significant increases relative to vehicle-treated cells.

Western Blot Analysis^[4]

Cell Line:

sterol-starved human hepatoma HepG2 cells treated with mevastatin
Concentration:

0.5, 1, 2, 4 μM
Incubation Time:

14 h (following 24 h mevastatin pretreatment)
Result:

Combination treatment with MK-2206 and mevastatin markedly increased LDLR protein levels relative to treatment with either agent alone.

Real Time qPCR^[4]

Cell Line:

sterol-fed human hepatoma HepG2 cells
Concentration:

5 μM
Incubation Time:

2-24 h
Result:

Induced mRNA expression of PCSK9, HMGCR, SREBP-2, and HMGCS1 in a time-dependent manner, with significant increases observed at multiple time points.
Had no effect on IDOL mRNA levels, and only modestly induced ACACA, FASN, and SCD1 mRNA levels with significant increases at 8 h.

Real Time qPCR^[4]

Cell Line:

sterol-fed human hepatoma HepG2 cells with SREBP-2 knockdown
Concentration:

5 μM
Incubation Time:

14 h (24 h post-transfection)
Result:

Knockdown of SREBP-2 significantly diminished the LDLR-inducing effect of MK-2206 on LDLR mRNA levels.

Western Blot Analysis^[4]

Cell Line:

sterol-fed human hepatoma HepG2 cells
Concentration:

5 μM
Incubation Time:

2, 4, 6 h
Result:

Increased levels of CTF-SREBP-2 (membrane fraction) and NTF-SREBP-2 (nuclear extract), with a concomitant reduction in FL-SREBP-2 levels, indicating enhanced proteolytic cleavage of SREBP-2.

In Vivo

MK-2206 free base enhances the anti-tumor efficacy of multiple chemotherapeutic agents in preclinical ovarian cancer xenograft models and induces mild, transient hyperglycemia and hyperinsulinemia in animals that resolves post-treatment^[1].
MK-2206 (120 mg/kg; p.o.; alternate days; 4 total doses) free base alleviates UUO-induced renal fibrosis in mice by reducing inflammation, inhibiting epithelial-mesenchymal transition, suppressing myofibroblast activation and extracellular matrix deposition, and blocking activation of the Akt/mTOR signaling pathway^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

strain not specified (n=6 per group)^[3]
Dosage:

120 mg/kg
Administration:

p.o.; alternate days; 4 total doses
Result:

Reduced renal tubular injury and interstitial collagen fiber deposition compared to untreated UUO mice.
Lowered renal mRNA expression of inflammatory factors TGF-β1, IL-1β, and IL-6.
Restored renal E-cadherin expression, reduced expression of myofibroblast markers α-SMA and Vimentin, and downregulated EMT-associated transcription factors Twist and Snail in UUO kidneys.
Decreased renal protein expression of extracellular matrix components Collagen I and Fibronectin.
Inhibited UUO-induced phosphorylation of Akt and mTOR in kidney tissue, reducing the p-Akt/Akt ratio and p-mTOR/mTOR ratio compared to untreated UUO mice.

Chemical Information

CAS No. 1032349-93-1
Molecular Weight 407.47
Formula C₂₅H₂₁N₅O
SMILES

O=C1N2C(C3=CC(C4=CC=CC=C4)=C(C(C=C5)=CC=C5C6(CCC6)N)N=C3C=C2)=NN1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Publications (483)

All In Vivo ImagingWBIFCell Proliferation/Viability Assay

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Journal Impact Factor

Signal Transduct Target Ther

Tet methylcytosine dioxygenase 2 (TET2) deficiency elicits EGFR-TKI (tyrosine kinase inhibitors) resistance in non-small cell lung cancer. [Abstract]

2024 Mar 9;9(1):65. PMID: 38461173
Signal Transduct Target Ther

M2 macrophages, but not M1 macrophages, support megakaryopoiesis by upregulating PI3K-AKT pathway activity. [Abstract]

2021 Jun 18;6(1):234. PMID: 34140465
Nature

Suppression of insulin feedback enhances the efficacy of PI3K inhibitors. [Abstract]

2018 Aug;560(7719):499-503. PMID: 30051890
Science

Interleukin-17 governs hypoxic adaptation of injured epithelium. [Abstract]

2022 Jul 8;377(6602):eabg9302. PMID: 35709248
Cancer Cell

Macropinocytosis maintains CAF subtype identity under metabolic stress in pancreatic cancer. [Abstract]

2025 Jul 15:S1535-6108(25)00271-5. PMID: 40712568
Cancer Cell

Liver Cancer Initiation Requires p53 Inhibition by CD44-Enhanced Growth Factor Signaling. [Abstract]

2018 Jun 11;33(6):1061-1077.e6. PMID: 29894692

MK-2206 free base purchased from MedChemExpress. Usage Cited in: Cancer Cell. 2018 Jun 11;33(6):1061-1077.e6. [Abstract]

Dih10 cells are treated with CDDP (20 μM) in the presence or absence of the Akt inhibitor MK2206 (5 μM).
Cell

Spatial control of the TSC complex integrates insulin and nutrient regulation of mTORC1 at the lysosome. [Abstract]

2014 Feb 13;156(4):771-85. PMID: 24529379

MK-2206 free base purchased from MedChemExpress. Usage Cited in: Cell. 2014 Feb 13;156(4):771-85. [Abstract]

(A) Effects of inhibiting PI3K and Akt in MEFs. Serum starved (16 hr) MEFs are pretreated (30 min) with Wortmannin (100 nM), MK2206 (2 μM) or vehicle (DMSO). Immunoblots of lysates are probed with the indicated antibodies. (B) PTEN null MEFs exhibit constitutive Akt, TSC2 and S6K phosphorylation, which are reversed by the Akt inhibitor MK2206.
Mol Cancer

LncRNA-HGBC stabilized by HuR promotes gallbladder cancer progression by regulating miR-502-3p/SET/AKT axis. [Abstract]

2019 Nov 21;18(1):167. PMID: 31752906
Nat Cancer

MEF2D-expressing cancer precursors reprogram tissue-resident macrophages to support liver tumorigenesis. [Abstract]

2025 Dec;6(12):1955-1975. PMID: 41131393
Nat Cancer

Targeting a lineage-specific PI3Kɣ-Akt signaling module in acute myeloid leukemia using a heterobifunctional degrader molecule. [Abstract]

2024 Jul;5(7):1082-1101. PMID: 38816660

MK-2206 free base purchased from MedChemExpress. Usage Cited in: Nat Cancer. 2024 Jul;5(7):1082-1101. [Abstract]

AKT phosphorylation levels by western blot in OCI-AML2 cells either infected with PIK3CG- and PIK3R5-directed sgRNAs for 72 hours or treated with 500nM IPI-549, AZ2, or the AKT inhibitor, MK-2206, for one and 72 hours.

MK-2206 free base purchased from MedChemExpress. Usage Cited in: Nat Cancer. 2024 Jul;5(7):1082-1101. [Abstract]

AKT phosphorylation levels by western blot of OCI-AML2 cells treated for one hour, 72 hours, or 72 hours followed by fresh addition of 500nM IPI-549, AZ2, or MK-2206 for one hour.
Immunity

Activating an interleukin 4-FLT3-STAT6 axis in multipotent progenitors restores lymphopoiesis in inflammation and aging. [Abstract]

2026 May 29:S1074-7613(26)00180-9. PMID: 42214327
Cell Stem Cell

Brain Endothelial Cells Maintain Lactate Homeostasis and Control Adult Hippocampal Neurogenesis. [Abstract]

2019 Dec 5;25(6):754-767.e9. PMID: 31761722
Bioact Mater

Orchestration of energy metabolism and osteogenesis by Mg2+ facilitates low-dose BMP-2-driven regeneration. [Abstract]

2022 Mar 24;18:116-127. PMID: 35387176
Cell Mol Immunol

β-adrenoreceptor-triggered PKA activation negatively regulates the innate antiviral response. [Abstract]

2023 Feb;20(2):175-188. PMID: 36600052
Nat Aging

GDF1 ameliorates cognitive impairment induced by hearing loss. [Abstract]

2024 Apr;4(4):568-583. PMID: 38491289
Cancer Res

NSD1-Mediated PPARγ Methylation Enhances PTEN Activity to Suppress Glycolysis and Tumor Progression in Endometrial Cancer. [Abstract]

2026 Mar 2. PMID: 41770031
Mol Cell

OXCT1 succinylation and activation by SUCLA2 promotes ketolysis and liver tumor growth. [Abstract]

2025 Feb 20;85(4):843-856.e6. PMID: 39862868
Mol Cell

PI3Kβ functions as a protein kinase to promote cellular protein O-GlcNAcylation and acetyl-CoA production for tumor growth. [Abstract]

2025 Mar 19:S1097-2765(25)00186-8. PMID: 40132583
Mol Cell

DNA damage promotes HLA class I presentation by stimulating a pioneer round of translation-associated antigen production. [Abstract]

2022 Jul 21;82(14):2557-2570.e7. PMID: 35594857
Cancer Res

Genomic Alterations in PIK3CA-Mutated Breast Cancer Result in mTORC1 Activation and Limit the Sensitivity to PI3Kα Inhibitors. [Abstract]

2021 May 1;81(9):2470-2480. PMID: 33685991
Mol Cell

RIPK1 Promotes Energy Sensing by the mTORC1 Pathway. [Abstract]

2021 Jan 21;81(2):370-385.e7. PMID: 33271062

MK-2206 free base purchased from MedChemExpress. Usage Cited in: Mol Cell. 2021 Jan 21;81(2):370-385.e7. [Abstract]

Lack of differential inhibition of mTORC1 signaling in wild-type versus RIPK1^−/− HT-29 cells upon inhibition of Akt by MK-2206 and mTORC1 by rapamycin or Torin-1. Cells were treated for indicated time points, and cell lysates were immunoblotted with indicated antibodies.
Nat Commun

INSIG1/2 succination mediated by the moonlighting function of ADSL promotes lipogenesis and liver tumorigenesis. [Abstract]

2026 Mar 15;17(1):4002. PMID: 41833955
Nat Commun

The UFL1-AKT positive feedback loop promotes breast cancer progression by enhancing lipid synthesis. [Abstract]

2026 Jan 20;17(1):614. PMID: 41559041
Nat Commun

Astrocytic pleiotrophin deficiency in the prefrontal cortex contributes to stress-induced depressive-like responses in male mice. [Abstract]

2025 Mar 14;16(1):2528. PMID: 40087317
Nat Commun

Astrocyte-derived clusterin disrupts glial physiology to obstruct remyelination in mouse models of demyelinating diseases. [Abstract]

2024 Sep 6;15(1):7791. PMID: 39242637
Nat Commun

Low potassium activation of proximal mTOR/AKT signaling is mediated by Kir4.2. [Abstract]

2024 Jun 17;15(1):5144. PMID: 38886379

MK-2206 free base purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Jun 17;15(1):5144. [Abstract]

Representative Western blots from isolated tubule suspensions cultured for 30 min in indicated K+ concentrations with or without the AKT inhibitor MK2206 (10 μM).
Nat Commun

NLRP6 potentiates PI3K/AKT signalling by promoting autophagic degradation of p85α to drive tumorigenesis. [Abstract]

2023 Sep 28;14(1):6069. PMID: 37770465
Nat Commun

IL6 supports long-term expansion of hepatocytes in vitro. [Abstract]

2022 Nov 29;13(1):7345. PMID: 36446858
Nat Commun

Engineering micro oxygen factories to slow tumour progression via hyperoxic microenvironments. [Abstract]

2022 Aug 2;13(1):4495. PMID: 35918337
Nat Commun

A loss-of-adhesion CRISPR-Cas9 screening platform to identify cell adhesion-regulatory proteins and signaling pathways. [Abstract]

2022 Apr 19;13(1):2136. PMID: 35440579
Nat Commun

Selective inhibition of cancer cell self-renewal through a Quisinostat-histone H1.0 axis. [Abstract]

2020 Apr 14;11(1):1792. PMID: 32286289
Nat Commun

SETBP1 accumulation induces P53 inhibition and genotoxic stress in neural progenitors underlying neurodegeneration in Schinzel-Giedion syndrome. [Abstract]

2021 Jun 30;12(1):4050. PMID: 34193871

MK-2206 free base purchased from MedChemExpress. Usage Cited in: Nat Commun. 2021 Jun 30;12(1):4050. [Abstract]

Total and phosphorylated AKT (pAKT Ser473) immunoblotting in isogenic control (+vehicle) and mutant NPCs (+vehicle or MK-2206 AKT inhibitor) and quantification as pAKT/AKT ratio.
Nat Commun

Recurrent hotspot mutations in HRAS Q61 and PI3K-AKT pathway genes as drivers of breast adenomyoepitheliomas. [Abstract]

2018 May 8;9(1):1816. PMID: 29739933

MK-2206 free base purchased from MedChemExpress. Usage Cited in: Nat Commun. 2018 May 8;9(1):1816. [Abstract]

Representative western blot analysis of p-ERK1/2, p-p90 RSK, p-AKT, p-AKT p-PRAS40, p-FOXO1/3a/4, p-GSK3β, p-mTOR, p-p70 S6K, and p-S6 protein in MCF-10A^P and MCF-10A^H1047R cells stably expressing empty vector (EV) or mutant HRAS^Q61R treated with 2 µM MK2206 (AKTi) at different time points.
Cell Death Differ

Matrix stiffness-induced YEATS2 drives HCC progression via epigenetic activation of the TGFBR2-TAZ-AKT pathway. [Abstract]

2026 Mar 3. PMID: 41776086
Cell Death Differ

Adaptor protein HIP-55-mediated signalosome protects against ferroptosis in myocardial infarction. [Abstract]

2023 Mar;30(3):825-838. PMID: 36639542
Cell Death Differ

CHIP-mediated CIB1 ubiquitination regulated epithelial-mesenchymal transition and tumor metastasis in lung adenocarcinoma. [Abstract]

2021 Mar;28(3):1026-1040. PMID: 33082516
Bone Res

Targeting AKT as a promising strategy for SOX2-positive, chemoresistant osteosarcoma. [Abstract]

2025 Feb 24;13(1):25. PMID: 39994220
Acta Pharm Sin B

HYD-PEP06 suppresses hepatocellular carcinoma metastasis, epithelial-mesenchymal transition and cancer stem cell-like properties by inhibiting PI3K/AKT and WNT/ β-catenin signaling activation. [Abstract]

2021 Jun;11(6):1592-1606. PMID: 34221870

MK-2206 free base purchased from MedChemExpress. Usage Cited in: Acta Pharm Sin B. 2021 Jun;11(6):1592-1606. [Abstract]

HCCLM3 cells were pretreated with or without 10 μmol/L MK2206 (a PI3K/AKT inhibitor) and then with HYD-PEP06 (200 μg/mL) for 24 h. The protein expression of p-AKT, AKT, the epithelial and mesenchymal markers was determined by Western blot.
Acta Pharm Sin B

Activated PKB/GSK-3 β synergizes with PKC- δ signaling in attenuating myocardial ischemia/reperfusion injury via potentiation of NRF2 activity: Therapeutic efficacy of dihydrotanshinone-I. [Abstract]

2021 Jan;11(1):71-88. PMID: 33532181

MK-2206 free base purchased from MedChemExpress. Usage Cited in: Acta Pharm Sin B. 2021 Jan;11(1):71-88. [Abstract]

Protein phosphorylation of PKB (Ser473) and GSK-3β (Ser9) in cardiomyocytes treated with DT or MK-2206 or DIF-3, either alone or in combination (n = 5).
Sci Transl Med

PP2A inhibition is a druggable MEK inhibitor resistance mechanism in KRAS-mutant lung cancer cells. [Abstract]

2018 Jul 18;10(450):eaaq1093. PMID: 30021885
Autophagy

2026 Apr 27. PMID: 42041131
J Mater Sci Technol

13 August 2022.
Adv Sci (Weinh)

EGR Proteins Mediate Interferon-Independent Anti-HSV-1 Responses Through Viral and Host Targets. [Abstract]

2026 Mar;13(13):e15546. PMID: 41486724
Adv Sci (Weinh)

Calhm6 Governs Macrophage Polarization Through Chp1-Camk4-Creb1 Axis and Ectosomal Delivery in Inflammatory Responses. [Abstract]

2025 Sep 26:e02395. PMID: 40999918
Adv Sci (Weinh)

AKT1 Phosphorylates FDX1 to Promote Cuproptosis Resistance in Triple-Negative Breast Cancer. [Abstract]

2025 Feb 20:e2408106. PMID: 39976173
Adv Sci (Weinh)

Targeting FDFT1 Reduces Cholesterol and Bile Acid Production and Delays Hepatocellular Carcinoma Progression Through the HNF4A/ALDOB/AKT1 Axis. [Abstract]

2025 Feb 3:e2411719. PMID: 39899681
Adv Sci (Weinh)

TRIM24 Cooperates with Ras Mutation to Drive Glioma Progression through snoRNA Recruitment of PHAX and DNA-PKcs. [Abstract]

2024 Jun 3:e2400023. PMID: 38828688
Adv Sci (Weinh)

Brain Metastasis from EGFR-Mutated Non-Small Cell Lung Cancer: Secretion of IL11 from Astrocytes Up-Regulates PDL1 and Promotes Immune Escape. [Abstract]

2024 Jul;11(26):e2306348. PMID: 38696655

MK-2206 free base purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2024 Jul;11(26):e2306348. [Abstract]

MK‐2206 (5 µM; 18 h) significantly suppresses IL11‐induced upregulation of PDL1, but did not affect the level of p‐EGFR.
Nat Chem Biol

Profiling drugs for rheumatoid arthritis that inhibit synovial fibroblast activation. [Abstract]

2017 Jan;13(1):38-45. PMID: 27820799
Leukemia

BCR::ABL1 tyrosine kinase inhibitors induce ribosome collisions to activate ZAK-dependent ribotoxic stress and apoptosis in chronic myeloid leukemia. [Abstract]

2026 May;40(5):955-969. PMID: 41912913
Leukemia

A Perturb-seq map of a differentiation hub reveals synergistic vulnerabilities in KMT2A-rearranged acute myeloid leukemia. [Abstract]

2026 Mar 25. PMID: 41882099
Theranostics

Endosome-microautophagy targeting chimera (eMIATAC) for targeted proteins degradation and enhance CAR-T cell anti-tumor therapy. [Abstract]

2024 Jul 22;14(11):4481-4498. PMID: 39113807
Theranostics

CBX4 suppresses CD8+ T cell antitumor immunity by reprogramming glycolytic metabolism. [Abstract]

2024 Jun 17;14(10):3793-3809. PMID: 38994031
Theranostics

ILT4 inhibition prevents TAM- and dysfunctional T cell-mediated immunosuppression and enhances the efficacy of anti-PD-L1 therapy in NSCLC with EGFR activation. [Abstract]

2021 Jan 19;11(7):3392-3416. PMID: 33537094
Theranostics

Arrb2 promotes endothelial progenitor cell-mediated postischemic neovascularization. [Abstract]

2020 Aug 6;10(21):9899-9912. PMID: 32863967
Theranostics

FGD1 promotes tumor progression and regulates tumor immune response in osteosarcoma via inhibiting PTEN activity. [Abstract]

2020 Feb 3;10(6):2859-2871. PMID: 32194840
Theranostics

3'-epi-12β-hydroxyfroside, a new cardenolide, induces cytoprotective autophagy via blocking the Hsp90/Akt/mTOR axis in lung cancer cells. [Abstract]

2018 Feb 15;8(7):2044-2060. PMID: 29556372
Chem Eng J

2025 Sep 7;522:168177.
J Adv Res

An injectable nano-hydroxyapatite-incorporated hydrogel with sustained release of Notoginsenoside R1 enhances bone regeneration by promoting angiogenesis through Notch1/Akt signaling. [Abstract]

2025 May 13:S2090-1232(25)00343-1. PMID: 40373960
Biomaterials

Nano-sensitizer with self-amplified drug release and hypoxia normalization properties potentiates efficient chemoradiotherapy of pancreatic cancer. [Abstract]

2024 Oct:310:122634. PMID: 38823195
Exp Mol Med

Protective effect of hepatocyte-enriched lncRNA-Mir122hg by promoting hepatocyte proliferation in acute liver injury. [Abstract]

2022 Nov;54(11):2022-2035. PMID: 36424455
J Exp Clin Cancer Res

AR antagonists develop drug resistance through TOMM20 autophagic degradation-promoted transformation to neuroendocrine prostate cancer. [Abstract]

2023 Aug 10;42(1):204. PMID: 37563661
J Exp Clin Cancer Res

A novel FBW7/NFAT1 axis regulates cancer immunity in sunitinib-resistant renal cancer by inducing PD-L1 expression. [Abstract]

2022 Jan 26;41(1):38. PMID: 35081978
J Exp Clin Cancer Res

The IRF2/CENP-N/AKT signaling axis promotes proliferation, cell cycling and apoptosis resistance in nasopharyngeal carcinoma cells by increasing aerobic glycolysis. [Abstract]

2021 Dec 10;40(1):390. PMID: 34893086
J Exp Clin Cancer Res

Cooperation between liver-specific mutations of pten and tp53 genetically induces hepatocarcinogenesis in zebrafish. [Abstract]

2021 Aug 20;40(1):262. PMID: 34416907
J Exp Clin Cancer Res

SKP2 promotes breast cancer tumorigenesis and radiation tolerance through PDCD4 ubiquitination. [Abstract]

2019 Feb 13;38(1):76. PMID: 30760284
J Exp Clin Cancer Res

Radiotherapy-induced cell death activates paracrine HMGB1-TLR2 signaling and accelerates pancreatic carcinoma metastasis. [Abstract]

2018 Apr 3;37(1):77. PMID: 29615080
J Nanobiotechnology

Small extracellular vesicles of hypoxic endothelial cells regulate the therapeutic potential of adipose-derived mesenchymal stem cells via miR-486-5p/PTEN in a limb ischemia model. [Abstract]

2022 Sep 24;20(1):422. PMID: 36153544
Sci Adv

Adissp activates insulin-independent glucose disposal and energy expenditure in white fat to treat diabetes and cardiometabolic disease. [Abstract]

2026 Apr 24;12(17):eaed2780. PMID: 42030391
Sci Adv

SRC kinase drives multidrug resistance induced by KRAS-G12C inhibition. [Abstract]

2024 Dec 13;10(50):eadq4274. PMID: 39661665
Carbohydr Polym

Inulin-like polysaccharide ABWW may impede CCl4 induced hepatic stellate cell activation through mediating the FAK/PI3K/AKT signaling pathway in vitro & in vivo. [Abstract]

2024 Feb 15:326:121637. PMID: 38142102
Sci Adv

Fasting improves therapeutic response in hepatocellular carcinoma through p53-dependent metabolic synergism. [Abstract]

2022 Jan 21;8(3):eabh2635. PMID: 35061544
Small

Tetrahedral DNA Nanostructure-Modified Nanocoating for Improved Bioaffinity and Osseointegration of Titanium. [Abstract]

2025 Mar 19:e2412747. PMID: 40103513
J Biomed Sci

Fusobacterium nucleatum promotes colorectal cancer liver metastasis via miR-5692a/IL-8 axis by inducing epithelial-mesenchymal transition. [Abstract]

2025 Jan 6;32(1):5. PMID: 39757156
Redox Biol

Phactr4 promotes oxidative stress and behavioral disorder caused by chronic stress via regulating PP1/GSK3-β pathway. [Abstract]

2025 Sep 16:87:103873. PMID: 40967008
Redox Biol

A detrimental role of NLRP6 in host iron metabolism during Salmonella infection. [Abstract]

2022 Feb:49:102217. PMID: 34942528
MedComm (2020)

Ubiquitin-specific protease 22 controls melanoma metastasis and vulnerability to ferroptosis through targeting SIRT1/PTEN/PI3K signaling. [Abstract]

2024 Aug 12;5(8):e684. PMID: 39135915
Pharmacol Res

CDC7 stabilized by KRAS signaling reactivation impairs chemosensitivity in KRAS-mutant colorectal cancer. [Abstract]

2025 Dec:222:108027. PMID: 41207349
Int J Surg

2026 Mar 11.
Nat Struct Mol Biol

ERK-USP9X-coupled regulation of thymidine kinase 1 promotes both its enzyme activity-dependent and its enzyme activity-independent functions for tumor growth. [Abstract]

2025 May;32(5):853-863. PMID: 39824978
Cancer Lett

Forkhead box protein FOXK1 disrupts the circadian rhythm to promote breast tumorigenesis in response to insulin resistance. [Abstract]

2024 Jul 31:217147. PMID: 39094826
J Neuroinflammation

Inhibition of ANGPTL8 protects against diabetes-associated cognitive dysfunction by reducing synaptic loss via the PirB signaling pathway. [Abstract]

2024 Aug 2;21(1):192. PMID: 39095838
Cancer Lett

FBXO43 promotes cell cycle progression in cancer cells through stabilizing SKP2. [Abstract]

2024 Jun 1:591:216848. PMID: 38604312
J Neuroinflammation

Human umbilical cord-derived mesenchymal stem cell transplantation supplemented with curcumin improves the outcomes of ischemic stroke via AKT/GSK-3β/β-TrCP/Nrf2 axis. [Abstract]

2023 Feb 24;20(1):49. PMID: 36829224

MK-2206 free base purchased from MedChemExpress. Usage Cited in: J Neuroinflammation. 2023 Feb 24;20(1):49. [Abstract]

Representative images of immunofluorescence analysis of Nrf2 expression in microglia treated with the AKT inhibitor MK2206 (5 μM，6 h), the GSK3β activator sodium nitroprusside (SNP, 100 μM， 6h), and the GSK3β inhibitor TDZD-8 (5 μM，6 h). Nrf2 (red), nuclei (blue).

MK-2206 free base purchased from MedChemExpress. Usage Cited in: J Neuroinflammation. 2023 Feb 24;20(1):49. [Abstract]

Representative western blots and quantitative data for the expression of Nrf2 in the nucleus and the downstream proteins of Nrf2 pathway, including HO-1 and NQO1, with different treatments (MK2206: 5 μM，6 h; SNP: 100 μM， 6h; TDZD-8: 5 μM，6 h).

MK-2206 free base purchased from MedChemExpress. Usage Cited in: J Neuroinflammation. 2023 Feb 24;20(1):49. [Abstract]

Nrf2 knockout abolished neurological function improvement mediated by combined curcumin-hUC-MSC treatment. TTC-stained brain sections (A) and quantitative analysis (B) showed the decreased infarct volume with combined therapy in MCAO mice (MK2206, SNP or TDZD-8 :5 mg/kg， intraperitoneally，3 consecutive days after MCAO).
Cancer Lett

Synergistic efficacy of telomerase-specific oncolytic adenoviral therapy and histone deacetylase inhibition in human hepatocellular carcinoma. [Abstract]

2023 Mar 1:556:216063. PMID: 36669725
Cancer Lett

Vimentin binds to a novel tumor suppressor protein, GSPT1-238aa, encoded by circGSPT1 with a selective encoding priority to halt autophagy in gastric carcinoma. [Abstract]

2022 Oct 1:545:215826. PMID: 35839920
Cancer Lett

CXCL13 promotes intestinal tumorigenesis through the activation of epithelial AKT signaling. [Abstract]

2021 Jul 28:511:1-14. PMID: 33894331
Cancer Lett

Targeting the EphB4 receptor tyrosine kinase sensitizes HER2-positive breast cancer cells to Lapatinib. [Abstract]

2020 Apr 10;475:53-64. PMID: 32006616
Int J Biol Sci

Targeting PLCG2 Suppresses Tumor Progression, Orchestrates the Tumor Immune Microenvironment and Potentiates Immune Checkpoint Blockade Therapy for Colorectal Cancer. [Abstract]

2024 Oct 14;20(14):5548-5575. PMID: 39494327
Int J Biol Sci

TGFβ Governs the Pleiotropic Activity of NDRG1 in Triple-Negative Breast Cancer Progression. [Abstract]

2023 Jan 1;19(1):204-224. PMID: 36594086
Int J Biol Sci

Accelerated Bone Regeneration by Astragaloside IV through Stimulating the Coupling of Osteogenesis and Angiogenesis. [Abstract]

2021 Apr 24;17(7):1821-1836. PMID: 33994865
Cell Death Dis

mTORC2 inhibition reduces tumor burden via STAT1 activation and enhanced response to anti-PD-L1 therapy. [Abstract]

2025 Dec 22;16(1):922. PMID: 41429766
Cell Death Dis

Cancer-associated fibroblasts expressing FSTL3 promote vasculogenic mimicry formation and drive colon cancer malignancy. [Abstract]

2025 Oct 6;16(1):706. PMID: 41053124
Burns Trauma

Neuregulin-1, a member of the epidermal growth factor family, mitigates STING-mediated pyroptosis and necroptosis in ischaemic flaps. [Abstract]

2024 Jun 9:12:tkae035. PMID: 38855574
Cell Death Dis

Retinoic acid and RARγ maintain satellite cell quiescence through regulation of translation initiation. [Abstract]

2022 Sep 29;13(9):838. PMID: 36175396
Cell Death Dis

Leukemia inhibitory factor drives glucose metabolic reprogramming to promote breast tumorigenesis. [Abstract]

2022 Apr 19;13(4):370. PMID: 35440095
Cell Death Dis

The therapeutic value of SC66 in human renal cell carcinoma cells. [Abstract]

2020 May 11;11(5):353. PMID: 32393791
Cell Death Dis

Paeonol induces cytoprotective autophagy via blocking the Akt/mTOR pathway in ovarian cancer cells. [Abstract]

2019 Aug 13;10(8):609. PMID: 31406198
Cell Death Dis

S-nitrosylation of the Peroxiredoxin-2 promotes S-nitrosoglutathione-mediated lung cancer cells apoptosis via AMPK-SIRT1 pathway. [Abstract]

2019 May; 10(5): 329. PMID: 30988280
Cell Death Dis

Transient receptor potential channel 6 knockdown prevents apoptosis of renal tubular epithelial cells upon oxidative stress via autophagy activation. [Abstract]

2018 Oct 3;9(10):1015. PMID: 30282964

MK-2206 free base purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2018 Oct 3;9(10):1015. [Abstract]

Representative western blot images are showing the LC3, and the phosphorylated and total protein expression of Akt and ERK1/2 after treatment with H₂O₂ in the presence and absence of MK2206 (5 μM) and U0126 (25 μM).
Genes Dis

2025 Nov 6.
Genes Dis

Protein phosphatase 6 (Pp6) is crucial for regulatory T cell function and stability in autoimmunity. [Abstract]

2021 Aug 17;9(2):562-575. PMID: 35224167
Angiogenesis

Clioquinol inhibits angiogenesis by promoting VEGFR2 degradation and synergizes with AKT inhibition to suppress triple-negative breast cancer vascularization. [Abstract]

2025 Feb 3;28(2):13. PMID: 39899169
Proc Natl Acad Sci U S A

Chaperone-mediated autophagy is involved in the execution of ferroptosis. [Abstract]

2019 Feb 19;116(8):2996-3005. PMID: 30718432

MK-2206 free base purchased from MedChemExpress. Usage Cited in: Proc Natl Acad Sci U S A. 2019 Feb 19;116(8):2996-3005. [Abstract]

HT22 cells were pretreated with the indicated inhibitors (CDDO (Bardoxolone, HY-14909); T0070907 (HY-13202); Troglitazone (HY-50935 ); TPCA-1 (HY-10074); JAKi; STAT3i; AV-412 (HY-10346); and MK2206 (HY-10358)) and then treated with T/Z, T/S/Z, T/C/Z for 9 h or Glutamate, Erastin (T: 20 ng/ml; Z: 50 μM; S: 20 nM; C: 1 μg/ml; Glutamate: 50 mM; Erastin 10 μM) for 12 h. Cell viability was measured by CellTiterGlo assay.
Proc Natl Acad Sci U S A

Phosphoinositide 3-kinase inhibitors induce DNA damage through nucleoside depletion. [Abstract]

2016 Jul 26;113(30):E4338-47. PMID: 27402769

MK-2206 free base purchased from MedChemExpress. Usage Cited in: Proc Natl Acad Sci U S A. 2016 Jul 26;113(30):E4338-47. [Abstract]

HCC1937 cells are treated for 16 h with inhibitors as indicated. Immunoblotting of total cell lysates is performed with antibodies as indicated. Induction of PAR and H2ax phosphorylation (γH2ax) following treatment with inhibitors of pan-PI3K (BKM120, 1.5 μM), PI3Kα (BYL719, 3 μM; PIK75, 0.5 μM), PI3Kβ (TGX221, 30 μM), AZD2281 (5 μM), and inhibitors of AKT (MK2206, 1 μM), SGK (GSK650394, 10 μM), or MAPKK (GSK1120212, 5 nM).
Cell Commun Signal

Activation of mechanosensitive ion channel Piezo1 linking metabolic reprogramming and pro-inflammatory responses in hepatocellular carcinoma. [Abstract]

2025 Jun 13;23(1):280. PMID: 40514668
Cell Commun Signal

CDO1 phosphorylation is required for IL-6-induced tumor cell proliferation through governing cysteine availability. [Abstract]

2025 Apr 23;23(1):194. PMID: 40269955
Cell Commun Signal

The IL-33-ST2 axis plays a vital role in endometriosis via promoting epithelial-mesenchymal transition by phosphorylating β-catenin. [Abstract]

2024 Jun 10;22(1):318. PMID: 38858740
Int J Biol Macromol

2026 May:360:151752. PMID: 41935804
Int J Biol Macromol

Exosomal microRNA-20b-5p contributes to cytarabine resistance in acute myeloid leukemia via the microtubule-associated serine/threonine kinase-like-phosphatidylinositol 3-kinase-protein kinase B signaling axis. [Abstract]

2025 Dec;333(Pt 2):148750. PMID: 41197693
Int J Biol Macromol

Trained immunity driven by Enterococcus faecalis ribosomal protein S11 enhances antigen presentation and boosts influenza vaccine efficacy via nanoparticle delivery. [Abstract]

2025 May 15:144179. PMID: 40381785
Int J Biol Macromol

Insect chitosan derived from Hermetia illucens larvae suppresses adipogenic signaling and promotes the restoration of gut microbiome balance. [Abstract]

2024 Nov 27:138168. PMID: 39613084
Int J Biol Macromol

Cepharanthine inhibits African swine fever virus replication by suppressing AKT-associated pathways through disrupting Hsp90-Cdc37 complex. [Abstract]

2024 Oct 30:137070. PMID: 39486740
Int J Biol Macromol

Sufentanil-induced Nrf2 protein ameliorates cerebral ischemia-reperfusion injury through suppressing neural ferroptosis. [Abstract]

2024 Aug 26:135109. PMID: 39197624
Acta Pharmacol Sin

FGF10 mitigates doxorubicin-induced myocardial toxicity in mice via activation of FGFR2b/PHLDA1/AKT axis. [Abstract]

2023 Oct;44(10):2004-2018. PMID: 37225844
Acta Pharmacol Sin

The oncoprotein HBXIP promotes human breast cancer growth through down-regulating p53 via miR-18b/MDM2 and pAKT/MDM2 pathways. [Abstract]

2018 Nov;39(11):1787-1796. PMID: 30181579

MK-2206 free base purchased from MedChemExpress. Usage Cited in: Acta Pharmacol Sin. 2018 Nov;39(11):1787-1796. [Abstract]

Effects of LY294002 or AKT siRNA on the levels of total AKT, pAKT, phosphorylated PRAS40 and pMDM2 are examined by Western blot analysis in MCF-7 cells when HBXIP was overexpressed. Effects of MK-2206 or HBXIP siRNA on the levels of total AKT, pAKT, pPRAS40, and pMDM2 are examined by Western blot analysis in MCF-7-HBXIP cells.
Phytomedicine

2026 Aug:158:158377. PMID: 42284639
Phytomedicine

2026 Jul:156:158188. PMID: 42068872
Phytomedicine

2026 Jul:156:158199. PMID: 42030800
Phytomedicine

Atraric acid and atranorin inhibit breast cancer energy metabolism and immune evasion through PI3K/AKT/Bcl-xL. [Abstract]

2025 Dec:149:157560. PMID: 41297322
Phytomedicine

Schisandrin B regulates mitochondrial dynamics via AKT1 activation and mitochondrial targeting to ameliorate renal ischemia-reperfusion injury. [Abstract]

2025 Jun:141:156672. PMID: 40220406
Phytomedicine

Sophoricoside ameliorates methicillin-resistant Staphylococcus aureus-induced acute lung injury by inhibiting Bach1/Akt pathway. [Abstract]

2024 Jun 27:132:155846. PMID: 38964155
Phytomedicine

The kava chalcone flavokawain B exerts inhibitory activity and synergizes with BCL-2 inhibition in malignant B-cell lymphoma. [Abstract]

2023 Nov:120:155074. PMID: 37716033

MK-2206 free base purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2023 Nov:120:155074. [Abstract]

SUDHL-4 cells were pretreated with the pan-PI3K inhibitor LY294002 (20 µM), the Akt inhibitor MK2206 (HY-108232; 4 µM), or the GSK3β inhibitor AR-A014418 (HY-10512; 10 µM) for 1 h followed by treating with FKB (Flavokawain B; HY-N2132) at 2.5 µg/ml for 24 h. Cell viability was measured using the MTS assay and results were shown as the percentage relative to the vehicle-treated control. *p < 0.05, #p < 0.01, and †p < 0.001.

MK-2206 free base purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2023 Nov:120:155074. [Abstract]

Raji and Jeko-1 cells were pretreated with the pan-PI3K inhibitor LY294002 (20 µM), the Akt inhibitor MK2206 (HY-108232; 4 µM), or the GSK3β inhibitor AR-A014418 (HY-10512; 10 µM) for 1 h followed by treating with FKB (Flavokawain B; HY-N2132) at 2.5 µg/ml for 24 h. Cell viability was measured using the MTS assay and results were shown as the percentage relative to the vehicle-treated control. *p < 0.05, #p < 0.01, and †p < 0.001.
Phytomedicine

Quercetin induces MGMT+ glioblastoma cells apoptosis via dual inhibition of Wnt3a/β-Catenin and Akt/NF-κB signaling pathways. [Abstract]

2023 Sep:118:154933. PMID: 37451151
Phytomedicine

RNA-binding domain 2 of nucleolin is important for the autophagy induction of curcumol in nasopharyngeal carcinoma cells. [Abstract]

2023 Jul:115:154833. PMID: 37137203
Phytomedicine

Ginsenoside Rh2 attenuates CDAHFD-induced liver fibrosis in mice by improving intestinal microbial composition and regulating LPS-mediated autophagy. [Abstract]

2022 Jul;101:154121. PMID: 35489327
Phytomedicine

Aesculin suppresses the NLRP3 inflammasome-mediated pyroptosis via the Akt/GSK3β/NF-κB pathway to mitigate myocardial ischemia/reperfusion injury. [Abstract]

2021 Nov:92:153687. PMID: 34482222
Free Radic Biol Med

7,8-Dihydroxyflavone protects acetaminophen induced liver injury through activating PI3K/Akt/NRF2/GPX4 mediated ferroptosis suppression. [Abstract]

2025 Oct 30:242:275-287. PMID: 41173313
Free Radic Biol Med

BP5 alleviates endotoxemia-induced acute lung injury by activating Nrf2 via dual regulation of the Keap1-Nrf2 interaction and the Akt (Ser473)/GSK3β (Ser9)/Fyn pathway. [Abstract]

2022 Nov 20;193(Pt 1):304-318. PMID: 36272670
Food Res Int

δ-Tocotrienol re-sensitizes vemurafenib-resistant melanoma cells to BRAF inhibition via modulation of AKT signaling. [Abstract]

2026 Aug 31:238:119457. PMID: 42215124
Sci Total Environ

Parental exposure to Cypermethrin causes intergenerational toxicity in zebrafish offspring. [Abstract]

2024 May 22:935:173456. PMID: 38788937
Sci Total Environ

Chronic MC-LR exposure promoted Aβ and p-tau accumulation via regulating Akt/GSK-3β signal pathway. [Abstract]

2021 Nov 10;794:148732. PMID: 34323745
J Orthop Translat

Profibrotic macrophage-derived CXCL4 promotes pericyte-to-myofibroblast transition after spinal cord injury. [Abstract]

2026 Feb 11:56:101032. PMID: 41836554
Cell Syst

Torin2 Exploits Replication and Checkpoint Vulnerabilities to Cause Death of PI3K-Activated Triple-Negative Breast Cancer Cells. [Abstract]

2020 Jan 22;10(1):66-81.e11. PMID: 31812693
J Transl Med

Inhibition of DDR1 potentiates carbon ion radiotherapy by promoting ferroptosis and immunogenic death in head and neck squamous cell carcinoma. [Abstract]

2025 Sep 24;23(1):1011. PMID: 40993737
Biomed Pharmacother

Inhibition of the PI3K/AKT signaling pathway contributes to the anti-renal cell carcinoma effects of deoxyelephantopin. [Abstract]

2025 May 8:187:118136. PMID: 40344699
J Transl Med

NL101 synergizes with the BCL-2 inhibitor venetoclax through PI3K-dependent suppression of c-Myc in acute myeloid leukaemia. [Abstract]

2024 Sep 27;22(1):867. PMID: 39334157
J Transl Med

Resveratrol attenuates inflammation and fibrosis in rheumatoid arthritis-associated interstitial lung disease via the AKT/TMEM175 pathway. [Abstract]

2024 May 14;22(1):457. PMID: 38745204
Biomed Pharmacother

Hyaluronan delays human amniotic epithelial stem cell senescence by regulating CD44 isoform switch to activate AKT/mTOR signals. [Abstract]

2024 Jan:170:116100. PMID: 38159379
J Transl Med

VEGF-A enhances the cytotoxic function of CD4+ cytotoxic T cells via the VEGF-receptor 1/VEGF-receptor 2/AKT/mTOR pathway. [Abstract]

2023 Feb 3;21(1):74. PMID: 36737819
J Transl Med

Chemotherapy induces ACE2 expression in breast cancer via the ROS-AKT-HIF-1α signaling pathway: a potential prognostic marker for breast cancer patients receiving chemotherapy. [Abstract]

2022 Nov 5;20(1):509. PMID: 36335375
J Transl Med

NCAPG promotes the proliferation of hepatocellular carcinoma through the CKII-dependent regulation of PTEN. [Abstract]

2022 Jul 21;20(1):325. PMID: 35864529
Biomed Pharmacother

USP13 mediates PTEN to ameliorate osteoarthritis by restraining oxidative stress, apoptosis and inflammation via AKT-dependent manner. [Abstract]

2021 Jan:133:111089. PMID: 33378983
Biomed Pharmacother

High glucose inhibits myogenesis and induces insulin resistance by down-regulating AKT signaling. [Abstract]

2019 Dec;120:109498. PMID: 31634780
Cell Mol Gastroenterol Hepatol

Fibrinogen/AKT/Microfilament Axis Promotes Colitis by Enhancing Vascular Permeability. [Abstract]

2021;11(3):683-696. PMID: 33075564
Stem Cell Res Ther

Mesenchymal stem cells overexpressing neuropeptide S promote the recovery of rats with spinal cord injury by activating the PI3K/AKT/GSK3β signaling pathway. [Abstract]

2025 Feb 28;16(1):100. PMID: 40022159
Stem Cell Res Ther

Thymosin beta-4 improves endothelial function and reparative potency of diabetic endothelial cells differentiated from patient induced pluripotent stem cells. [Abstract]

2022 Jan 10;13(1):13. PMID: 35012642
Stem Cell Res Ther

Akt signaling is activated by TGFβ2 and impacts tenogenic induction of mesenchymal stem cells. [Abstract]

2021 Jan 26;12(1):88. PMID: 33499914
Oncogene

Aberrant JmjC domain-containing protein 8 (JMJD8) expression promotes activation of AKT and tumor epithelial-mesenchymal transition. [Abstract]

2020 Oct;39(41):6451-6467. PMID: 32879443
Oncogene

AKT drives SOX2 overexpression and cancer cell stemness in esophageal cancer by protecting SOX2 from UBR5-mediated degradation. [Abstract]

2019 Jun;38(26):5250-5264. PMID: 30894683

MK-2206 free base purchased from MedChemExpress. Usage Cited in: Oncogene. 2019 Jun;38(26):5250-5264. [Abstract]

Western blotting analysis shows the effect of AKT inhibitor MK2206 (AKT i) and SET7 inhibitor (R)-PFI-2 on the levels of SOX2 proteins. The cells are treated with 1 μM MK2206 for 24 h and 10 μM (R)-PFI-2 for 12 h before being harvested for analysis.
Oncogene

PIM2-mediated phosphorylation of hexokinase 2 is critical for tumor growth and paclitaxel resistance in breast cancer. [Abstract]

2018 Nov;37(45):5997-6009. PMID: 29985480
Oncogene

Abl kinase regulation by BRAF/ERK and cooperation with Akt in melanoma. [Abstract]

2017 Aug 10;36(32):4585-4596. PMID: 28368422
PLoS Biol

The mTORC2 subunit RICTOR drives breast cancer progression by promoting ganglioside biosynthesis through transcriptional and epigenetic mechanisms. [Abstract]

2025 Sep 11;23(9):e3003362. PMID: 40934285
Int Endod J

Fibroblast Dysregulation in Multiple Idiopathic External Cervical Resorption: Laboratory Investigation From a Rare Case of 13 Affected Teeth. [Abstract]

2025 Sep 23. PMID: 40988149
Blood Adv

Melanocortin/MC5R axis regulates the proliferation of hematopoietic stem cells in mice after ionizing radiation injury. [Abstract]

2023 Jul 11;7(13):3199-3212. PMID: 36920787
Eur J Cancer

The regulation of CD73 in non-small cell lung cancer. [Abstract]

2022 May 19;170:91-102. PMID: 35598361
Cell Death Discov

The protective up-regulation of metallothionein-2A in intervertebral disc degeneration inhibits nucleus pulposus cell ferroptosis through activation of the PI3K/AKT/mTOR pathway. [Abstract]

2026 Feb 25;12(1):111. PMID: 41741409
Cell Death Discov

Maternal linoleic acid-rich diet ameliorates bilirubin neurotoxicity in offspring mice. [Abstract]

2024 Jul 19;10(1):329. PMID: 39030174
Cell Death Discov

TGR5-HNF4α axis contributes to bile acid-induced gastric intestinal metaplasia markers expression. [Abstract]

2020 Jul 6;6:56. PMID: 32655894
Cell Rep

Collagen 1-mediated CXCL1 secretion in tumor cells activates fibroblasts to promote radioresistance of esophageal cancer. [Abstract]

2023 Oct 17;42(10):113270. PMID: 37851572
Cell Rep

Deciphering the functional landscape of phosphosites with deep neural network. [Abstract]

2023 Sep 1;42(9):113048. PMID: 37659078
Cell Rep

Multi-omics profiling visualizes dynamics of cardiac development and functions. [Abstract]

2022 Dec 27;41(13):111891. PMID: 36577384
Cell Rep

The deubiquitinase OTUD1 noncanonically suppresses Akt activation through its N-terminal intrinsically disordered region. [Abstract]

2023 Jan 31;42(1):111916. PMID: 36640312
Cell Rep

Renal Klotho and inorganic phosphate are extrinsic factors that antagonistically regulate hematopoietic stem cell maintenance. [Abstract]

2022 Feb 15;38(7):110392. PMID: 35172146
Br J Cancer

Deciphering the role of IGF2BP2 and PRMT5 in gallbladder cancer progression: insights from multi-omics analysis. [Abstract]

2025 Jun 24. PMID: 40555776
Br J Cancer

Activation of an AKT/FOXM1/STMN1 pathway drives resistance to tyrosine kinase inhibitors in lung cancer. [Abstract]

2017 Sep 26;117(7):974-983. PMID: 28850563

MK-2206 free base purchased from MedChemExpress. Usage Cited in: Br J Cancer. 2017 Sep 26;117(7):974-983. [Abstract]

The effect of the AKT inhibitor MK2206 (10 μM) on the expression levels of phosphor-AKT, AKT, and STMN1 in TKI-pretreated NCI-H460 cells. β-actin is used as a loading control.
Anal Chem

TROP2 Promotes Tumor Cell Migration through Downregulation of DSG2 Revealed by Super-Resolution Fluorescence Imaging. [Abstract]

2026 Jan 27;98(3):1901-1914. PMID: 41531208
Mol Metab

Linking metabolism and histone acetylation dynamics by integrated metabolic flux analysis of Acetyl-CoA and histone acetylation sites. [Abstract]

2024 Sep 19:102032. PMID: 39305948
Sci Signal

The Bacillus cereus toxin alveolysin disrupts the intestinal epithelial barrier by inducing microtubule disorganization through CFAP100. [Abstract]

2023 May 16;16(785):eade8111. PMID: 37192300
Antioxidants (Basel)

Sphingosine-1-Phosphate Alleviates Irradiation Induced Salivary Gland Hypofunction through Preserving Endothelial Cells and Resident Macrophages. [Abstract]

2022 Oct 18;11(10):2050. PMID: 36290773
Int J Radiat Oncol Biol Phys

Radiation Induces Pericyte-Myofibroblast Transition. [Abstract]

2025 Jun 16:S0360-3016(25)03903-3. PMID: 40532892
J Cell Biol

Membrane-bound SCF and VCAM-1 synergistically regulate the morphology of hematopoietic stem cells. [Abstract]

2021 Oct 4;220(10):e202010118. PMID: 34402812
Elife

Akt phosphorylates insulin receptor substrate to limit PI3K-mediated PIP3 synthesis. [Abstract]

2021 Jul 13:10:e66942. PMID: 34253290
Phytother Res

2026 Apr 27. PMID: 42037203
Oncoimmunology

Ex vivo AKT-inhibition facilitates generation of polyfunctional stem cell memory-like CD8+ T cells for adoptive immunotherapy. [Abstract]

2018 Aug 6;7(10):e1488565. PMID: 30288356
J Agric Food Chem

Platycodin D Ameliorates Cognitive Impairment in Type 2 Diabetes Mellitus Mice via Regulating PI3K/Akt/GSK3β Signaling Pathway. [Abstract]

2024 Jun 5;72(22):12516-12528. PMID: 38491972
J Agric Food Chem

Ginsenoside Rg2 Ameliorating CDAHFD-Induced Hepatic Fibrosis by Regulating AKT/mTOR-Mediated Autophagy. [Abstract]

2022 Feb 16;70(6):1911-1922. PMID: 35104139
J Agric Food Chem

Stachydrine Mediates Rapid Vascular Relaxation: Activation of Endothelial Nitric Oxide Synthase Involving AMP-Activated Protein Kinase and Akt Phosphorylation in Vascular Endothelial Cells. [Abstract]

2019 Sep 4;67(35):9805-9811. PMID: 31407895
JCI Insight

FXR shapes an immunosuppressive microenvironment in PD-L1lo/- non-small cell lung cancer by upregulating HVEM. [Abstract]

2025 Sep 23;10(18):e190716. PMID: 40985894
Hum Reprod

CDC42 deficiency leads to endometrial stromal cell senescence in recurrent implantation failure. [Abstract]

2024 Nov 1:deae246. PMID: 39487595
Ecotoxicol Environ Saf

Neohesperidin alleviates the inhibitory effect of bisphenol A on the myogenic differentiation of umbilical cord mesenchymal stem cells via the IGF1R/AKT1/RHOA signaling pathway. [Abstract]

2024 Jul 30:283:116804. PMID: 39083871
Ecotoxicol Environ Saf

Interleukin-37 relieves PM2.5-triggered lung injury by inhibiting autophagy through the AKT/mTOR signaling pathway in vivo and in vitro. [Abstract]

2024 Jan 1:269:115816. PMID: 38091678
Mol Ther Nucleic Acids

miR-199a-3p increases the anti-tumor activity of palbociclib in liver cancer models. [Abstract]

2022 Jul 20:29:538-549. PMID: 36035756
Mol Ther Nucleic Acids

Upregulation of miR-520c-3p via hepatitis B virus drives hepatocellular migration and invasion by the PTEN/AKT/NF-κB axis. [Abstract]

2022 May 20:29:47-63. PMID: 35795482
Cancer Cell Int

Short-term starvation synergistically enhances cytotoxicity of Niraparib via Akt/mTOR signaling pathway in ovarian cancer therapy. [Abstract]

2022 Jan 11;22(1):18. PMID: 35016681
Cancer Cell Int

Silencing of PSMC2 inhibits development and metastasis of prostate cancer through regulating proliferation, apoptosis and migration. [Abstract]

2021 Apr 26;21(1):235. PMID: 33902600
Cell Biol Toxicol

2026 May 21. PMID: 42165897
Transl Res

TRPV4 in adipose tissue ameliorates diet-induced obesity by promoting white adipocyte browning. [Abstract]

2024 Apr:266:16-31. PMID: 37926276
J Invest Dermatol

Cornulin Is Induced in Psoriasis Lesions and Promotes Keratinocyte Proliferation via Phosphoinositide 3-Kinase/Akt Pathways. [Abstract]

2019 Jan;139(1):71-80. PMID: 30009832

MK-2206 free base purchased from MedChemExpress. Usage Cited in: J Invest Dermatol. 2019 Jan;139(1):71-80. [Abstract]

HEKa (left) and HaCaT (right) cells are seeded in six-cell plates and pretreated with MK2206 for 6 hours, and then stimulated with M5 (2.5 ng/mL) for 48 hours before measuring the protein levels of cyclin D1, Akt, and phosphoAkt by Western blot analysis.
Biochem Pharmacol

Dopamine receptor D2 regulates inflammation and fibrosis in metabolic dysfunction-associated steatohepatitis disease. [Abstract]

2026 Mar:245:117696. PMID: 41519398
Biochem Pharmacol

Celastrol synergizes with MEK1 inhibitor nedometinib to overcome resistance in bladder cancer via dual suppression of CDK1/CDC5L and feedback-activated Akt/STAT3. [Abstract]

2025 Dec 10:245:117629. PMID: 41386559
J Ginseng Res

2025 Dec 29.
Biochem Pharmacol

Lasiokaurin suppresses breast cancer growth by blocking autophagic flux and regulating cellular energy homeostasis. [Abstract]

2025 Aug 7;242(Pt 3):117212. PMID: 40782951
Biochem Pharmacol

Dihydroartemisinin inhibits NSCLC by targeting MDM2/c-Myc axis independent of p53. [Abstract]

2025 Jul 26:241:117191. PMID: 40721007
Cell Prolif

SETD2 epidermal deficiency promotes cutaneous wound healing via activation of AKT/mTOR Signalling. [Abstract]

2021 Jun;54(6):e13045. PMID: 33949020
Neurobiol Dis

Rho-kinase inhibition has antidepressant-like efficacy and expedites dendritic spine pruning in adolescent mice. [Abstract]

2019 Apr;124:520-530. PMID: 30593834
Mol Cancer Ther

AXL kinase inhibition promotes cytosolic DNA sensor cGAS activity and sensitizes poorly immunogenic tumors to chemo-immunotherapy. [Abstract]

2025 Nov 20. PMID: 41263056
Mol Cancer Ther

AKT Inhibition Sensitizes to Polo-Like Kinase 1 Inhibitor Onvansertib in Prostate Cancer. [Abstract]

2024 Oct 1;23(10):1404-1417. PMID: 38894678
J Ethnopharmacol

2026 Oct 28:369:121888. PMID: 42202911
J Ethnopharmacol

Paris polyphylla Smith var. yunnanensis-derived saponins potentiate the antitumor activity of GPX4 inhibitors. [Abstract]

2026 Feb 28:357:120890. PMID: 41232632
J Ethnopharmacol

Targeting GRB2-Akt-SREBP1 signaling axis by saponins from Paris polyphylla Smith var. yunnanensis (Franch.) Hand.-Mazz to overcome cisplatin resistance in bladder cancer. [Abstract]

2025 Aug 27:354:120502. PMID: 40882789
J Ethnopharmacol

Mechanism and molecular targets of Euphorbia fischeriana Steud root extract in hepatocellular carcinoma. [Abstract]

2025 Mar 25:119707. PMID: 40147679
J Ethnopharmacol

Network pharmacology and phytochemical composition combined with validation in vivo and in vitro reveal the mechanism of platycodonis radix ameliorating PM2.5-induced acute lung injury. [Abstract]

2024 Sep 13:118829. PMID: 39278295
J Ethnopharmacol

Moluodan promotes DSS-induced intestinal inflammation involving the reprogram of macrophage function and polarization. [Abstract]

2024 Feb 10:320:117393. PMID: 37952735
J Ethnopharmacol

Bolbostemma paniculatum (Maxim.) Franquet extract suppresses the development of colorectal cancer through downregulation of PI3K/Akt pathway. [Abstract]

2022 Apr 6:287:114937. PMID: 34958876
J Ethnopharmacol

Astragalus saponins improves stroke by promoting the proliferation of neural stem cells through phosphorylation of Akt. [Abstract]

2021 Sep 15:277:114224. PMID: 34044075
Food Funct

Hesperetin ameliorates hepatic oxidative stress and inflammation via the PI3K/AKT-Nrf2-ARE pathway in oleic acid-induced HepG2 cells and a rat model of high-fat diet-induced NAFLD. [Abstract]

2021 May 11;12(9):3898-3918. PMID: 33977953
Cells

Integrin-Mediated TIMP1 Signaling Reprograms Liver Macrophages and Accelerates Colorectal Cancer Metastasis. [Abstract]

2025 Dec 23;15(1):29. PMID: 41511313
Cells

MK-2206 Alleviates Renal Fibrosis by Suppressing the Akt/mTOR Signaling Pathway In Vivo and In Vitro. [Abstract]

2022 Nov 5;11(21):3505. PMID: 36359901
Gastric Cancer

COPS5 inhibition synergizes with the antitumor effect of trastuzumab by PTEN upregulation in HER2-amplified gastric cancer. [Abstract]

2025 Oct 2. PMID: 41039054
Commun Biol

OPN3-mediated positive regulation of angiogenesis in HUVECs through VEGFR2 interaction. [Abstract]

2025 Mar 31;8(1):529. PMID: 40164822
Commun Biol

PI3K-AKT-mediated phosphorylation of Thr260 in CgCaspase-3/6/7 regulates heat-induced activation in oysters. [Abstract]

2024 Nov 7;7(1):1459. PMID: 39511363
Life Sci

Lactobacillus rhamnosus GG aggravates vascular calcification in chronic kidney disease: A potential role for extracellular vesicles. [Abstract]

2023 Oct 15:331:122001. PMID: 37625519
Life Sci

A bovine milk-derived peptide ameliorates alloxan-injured pancreatic β cells through IRS2/PI3K/Akt signaling. [Abstract]

2022 Nov 1:308:120907. PMID: 36037870
Life Sci

DHCR24 overexpression modulates microglia polarization and inflammatory response via Akt/GSK3β signaling in Aβ25-35 treated BV-2 cells. [Abstract]

2020 Nov 1;260:118470. PMID: 32950573
Life Sci

IGF-1 promotes multiple myeloma progression through PI3K/Akt-mediated epithelial-mesenchymal transition. [Abstract]

2020 May 15;249:117503. PMID: 32142767
Life Sci

Osteopontin induces atrial fibrosis by activating Akt/GSK-3β/β-catenin pathway and suppressing autophagy. [Abstract]

2020 Mar 15;245:117328. PMID: 31954162
Nutrients

Phosphate Metabolic Inhibition Contributes to Irradiation-Induced Myelosuppression through Dampening Hematopoietic Stem Cell Survival. [Abstract]

2022 Aug 18;14(16):3395. PMID: 36014901
Int J Mol Sci

Traditional Medicinal Plant Dahlia pinnata Selectively Suppresses TNF-α Expression Through Modulation of NF-κB and p38 Signaling. [Abstract]

2026 Jan 22;27(2):1122. PMID: 41596763
Int J Mol Sci

Hypercapnia Increases Influenza A Virus Infection of Bronchial Epithelial Cells by Augmenting Cellular Cholesterol via mTOR and Akt. [Abstract]

2025 Apr 26;26(9):4133. PMID: 40362373
Int J Mol Sci

Metformin Suppresses Stemness of Non-Small-Cell Lung Cancer Induced by Paclitaxel through FOXO3a. [Abstract]

2023 Nov 22;24(23):16611. PMID: 38068934
Int J Mol Sci

Involvement of the p38/MK2 Pathway in MCLR Hepatotoxicity Revealed through MAPK Pharmacological Inhibition and Phosphoproteomics in HepaRG Cells. [Abstract]

2023 Jul 6;24(13):11168. PMID: 37446360
Int J Mol Sci

PEBP4 Directs the Malignant Behavior of Hepatocellular Carcinoma Cells via Regulating mTORC1 and mTORC2. [Abstract]

2022 Aug 8;23(15):8798. PMID: 35955931
Int J Oncol

Semaphorin 4D promotes bone invasion in head and neck squamous cell carcinoma. [Abstract]

2017 Aug;51(2):625-632. PMID: 28656278
Cell Oncol (Dordr)

Blocking MMP-12-modulated epithelial-mesenchymal transition by repurposing penfluridol restrains lung adenocarcinoma metastasis via uPA/uPAR/TGF-β/Akt pathway. [Abstract]

2021 Oct;44(5):1087-1103. PMID: 34319576
Int Immunopharmacol

2026 Sep 1:184:116961. PMID: 42242136
Int Immunopharmacol

IL-5 promotes airway remodeling in asthma by mediating epithelial-mesenchymal transition via the AKT/NLRP3 pathway. [Abstract]

2026 Apr 15:175:116422. PMID: 41759267
Int Immunopharmacol

The mechanism study of isoorientin regulating neuroinflammation after subarachnoid hemorrhage through AKT/GSK3β. [Abstract]

2026 Apr 1:174:116299. PMID: 41671619
Int Immunopharmacol

Gasdermin D-dependent macrophage pyroptosis mediates polystyrene microplastics-induced pulmonary fibrosis. [Abstract]

2026 Mar 1:172:116189. PMID: 41539003
Bioorg Chem

Gypenoside L inhibits oral squamous cell carcinoma progression by targeting the AKT signaling pathway. [Abstract]

2026 Jan:168:109303. PMID: 41343873
Eur J Pharmacol

Investigating the effects of dendrobine on fibrosis and inflammation in orbital fibroblasts of thyroid-associated ophthalmopathy via network pharmacology, molecular docking, and transcriptomics. [Abstract]

2025 Jun 20:177871. PMID: 40544935
Int Immunopharmacol

CARD9 promotes cholangiocarcinoma by regulating the IL-17A/Hedgehog and the THEM4/AKT/mTOR signaling pathways. [Abstract]

2024 Oct 16;143(Pt 2):113399. PMID: 39418733
Int Immunopharmacol

The role of the PI3K/AKT/mTOR pathway in mediating PD-L1 upregulation during fibroblast transdifferentiation. [Abstract]

2024 Sep 18;142(Pt B):113186. PMID: 39298817
Int Immunopharmacol

Role of glucose metabolism reprogramming in keratinocytes in the link between psoriasis and metabolic syndrome. [Abstract]

2024 Jul 19:139:112704. PMID: 39032466
Mol Cancer Res

Vasorin exocytosed from glioma cells facilitates angiogenesis via VEGFR2/AKT signaling pathway. [Abstract]

2024 Jul 2;22(7):668-681. PMID: 38488456
Eur J Pharmacol

Licochalcone A induces mitochondria-dependent apoptosis and interacts with venetoclax in acute myeloid leukemia. [Abstract]

2024 Apr 5:968:176418. PMID: 38350590
Eur J Pharmacol

Platycodin D induces apoptotic cell death through PI3K/AKT and MAPK/ERK pathways and synergizes with venetoclax in acute myeloid leukemia. [Abstract]

2023 Oct 5:956:175957. PMID: 37541375
Int Immunopharmacol

Paeoniflorin promotes intestinal stem cell-mediated epithelial regeneration and repair via PI3K-AKT-mTOR signalling in ulcerative colitis. [Abstract]

2023 Jun:119:110247. PMID: 37159966
Int Immunopharmacol

Topoisomerase 2 inhibitor etoposide promotes interleukin-10 production in LPS-induced macrophages via upregulating transcription factor Maf and activating PI3K/Akt pathway. [Abstract]

2021 Dec;101(Pt A):108264. PMID: 34715493
Int Immunopharmacol

Z-guggulsterone induces PD-L1 upregulation partly mediated by FXR, Akt and Erk1/2 signaling pathways in non-small cell lung cancer. [Abstract]

2021 Apr:93:107395. PMID: 33529916
Mol Cancer Res

Targeting RICTOR Sensitizes SMAD4-Negative Colon Cancer to Irinotecan. [Abstract]

2020 Mar;18(3):414-423. PMID: 31932471
Biosci Rep

PD-L1 regulates tumorigenesis and autophagy of ovarian cancer by activating mTORC signaling. [Abstract]

2019 Dec 20;39(12):BSR20191041. PMID: 31799599
Eur J Pharmacol

AS1949490, an inhibitor of 5'-lipid phosphatase SHIP2, promotes protein kinase C-dependent stabilization of brain-derived neurotrophic factor mRNA in cultured cortical neurons. [Abstract]

2019 May 15:851:69-79. PMID: 30753865
Cancer Biol Ther

Daurisoline inhibits glycolysis of lung cancer by targeting the AKT-HK2 axis. [Abstract]

2025 Dec;26(1):2442556. PMID: 39699276
Molecules

Geniposidic Acid from Eucommia ulmoides Oliver Staminate Flower Tea Mitigates Cellular Oxidative Stress via Activating AKT/NRF2 Signaling. [Abstract]

2022 Dec 5;27(23):8568. PMID: 36500666
Toxicology

Environmental exposure to microcystin-LR increases the risks of urinary bladder proliferation and carcinogenesis: Evidence from case control, animal, and in vitro studies. [Abstract]

2022 Oct;480:153326. PMID: 36115647
Cancer Biol Ther

Repeated treatments of Capan-1 cells with PARP1 and Chk1 inhibitors promote drug resistance, migration and invasion. [Abstract]

2022 Dec 31;23(1):69-82. PMID: 35000525
Endocr Relat Cancer

MAPK- and AKT-activated thyroid cancers are sensitive to group I PAK inhibition. [Abstract]

2019 Aug;26(8):699-712. PMID: 31146260
Mol Oncol

PP2A-based triple-strike therapy overcomes mitochondrial apoptosis resistance in brain cancer cells. [Abstract]

2023 Sep;17(9):1803-1820. PMID: 37458534
Mol Oncol

L1CAM promotes vasculogenic mimicry formation by miR-143-3p-induced expression of hexokinase 2 in glioma. [Abstract]

2023 Apr;17(4):664-685. PMID: 36708044
Front Microbiol

Toll-like receptor 2 signaling pathway activation contributes to a highly efficient inflammatory response in Japanese encephalitis virus-infected mouse microglial cells by proteomics. [Abstract]

2022 Sep 12:13:989183. PMID: 36171749
Mol Oncol

Kaiso phosphorylation at threonine 606 leads to its accumulation in the cytoplasm, reducing its transcriptional repression of the tumour suppressor CDH1. [Abstract]

2022 Sep;16(17):3192-3209. PMID: 35851744
Cancers (Basel)

The Targeted Degradation of BRAF V600E Reveals the Mechanisms of Resistance to BRAF-Targeted Treatments in Colorectal Cancer Cells. [Abstract]

2023 Dec 12;15(24):5805. PMID: 38136350
Transplant Cell Ther

Thymosin β4 exerts cytoprotective function and attenuates liver injury in murine hepatic sinusoidal obstruction syndrome after hematopoietic stem cell transplantation. [Abstract]

2023 Aug;29(8):492.e1-492.e10. PMID: 37192732
Cancers

Expression of MALAT1 Promotes Trastuzumab Resistance in HER2 Overexpressing Breast Cancers. [Abstract]

2020 Jul 16;12(7):1918. PMID: 32708561
Clin Epigenetics

Retinoic acid-induced 2 (RAI2) is a novel tumor suppressor, and promoter region methylation of RAI2 is a poor prognostic marker in colorectal cancer. [Abstract]

2018 May 23:10:69. PMID: 29796120

MK-2206 free base purchased from MedChemExpress. Usage Cited in: Clin Epigenetics. 2018 May 23:10:69. [Abstract]

The expression levels of E-cadherin, vimentin, pan-AKT, p-AKTser473, MMP-2, MMP-7, MMP-9, and actin (control) are detected by Western blot in RAI2 un-expressed and re-expressed RKO and LOVO cells as well as in the control group, shRAI2 knockdown group, and shRAI2 knockdown plus MK2206 treated group.
Mol Neurobiol

Regulation of Melatonin on AQP4 Expression in Brain of SD Newborn Rats with Bilirubin Encephalopathy and Protection Mechanism. [Abstract]

2025 Dec 3;63(1):239. PMID: 41335355
ACS Omega

Dynamic Detection of the E3-PROTAC-Target Protein Ternary Complex In Vitro and In Vivo via Bimolecular Fluorescence Complementation. [Abstract]

2024 Dec 3;9(50):49739-49748. PMID: 39713624
ACS Omega

MGST1 Expression Is Associated with Poor Prognosis, Enhancing the Wnt/β-Catenin Pathway via Regulating AKT and Inhibiting Ferroptosis in Gastric Cancer. [Abstract]

2023 Jun 16;8(26):23683-23694. PMID: 37426275
Front Cell Dev Biol

Accelerated Bone Regeneration by Adrenomedullin 2 Through Improving the Coupling of Osteogenesis and Angiogenesis via β-Catenin Signaling. [Abstract]

2021 Apr 14:9:649277. PMID: 33937244
ACS Omega

Roles of Akt and ERK in mTOR-Dependent Antidepressant Effects of Vanillic Acid. [Abstract]

2020 Feb 13;5(7):3709-3716. PMID: 32118186
Cancer Sci

Reverse of non-small cell lung cancer drug resistance induced by cancer-associated fibroblasts via a paracrine pathway. [Abstract]

2018 Apr;109(4):944-955. PMID: 29383798

MK-2206 free base purchased from MedChemExpress. Usage Cited in: Cancer Sci. 2018 Apr;109(4):944-955. [Abstract]

Western blotting of Sox2 and b-actin in A549 cells with or without IGF2 treated in the presence or absence of MK-2206.
FASEB J

Rhythmic Insulin Induced by Eating During the Active Phase Promotes Skeletal Muscle Growth via Synchronizing Mitochondrial Energy Supply Rhythm and Cell Cycle. [Abstract]

2025 May 31;39(10):e70619. PMID: 40354091
J Cell Mol Med

Stromal Cell Derived Factor-1 Promotes Hepatic Insulin Resistance via Inhibiting Hepatocyte Lipophagy. [Abstract]

2025 Jan;29(2):e70352. PMID: 39855896
J Cell Mol Med

Foxk2 Enhances Adipogenic Differentiation by Relying on the Transcriptional Activation of Peroxisome Proliferator-Activated Receptor Gamma. [Abstract]

2025 Jan;29(1):e70332. PMID: 39789420
Lab Invest

HECW1-Mediated Ubiquitination of HIPK2 Drives Metastasis in Gastric Cancer Through the AKT Signaling Pathway. [Abstract]

2024 Nov 28:102202. PMID: 39615883
Lipids Health Dis

Knockdown of RASD1 improves MASLD progression by inhibiting the PI3K/AKT/mTOR pathway. [Abstract]

2024 Dec 27;23(1):424. PMID: 39731125
Biochim Biophys Acta Mol Basis Dis

JuA alleviates liver ischemia-reperfusion injury by activating AKT/NRF2/HO-1 pathways. [Abstract]

2024 Sep 3:167496. PMID: 39237046
Biochim Biophys Acta Mol Basis Dis

Resistance exercise preconditioning prevents disuse muscle atrophy by inhibiting apoptosis and protein degradation via SESN2 in C57BL/6J mice. [Abstract]

2024 Mar 2;1870(4):167111. PMID: 38432454
J Cell Mol Med

NEDD4L is a promoter for angiogenesis and cell proliferation in human umbilical vein endothelial cells. [Abstract]

2024 Apr;28(8):1-11. PMID: 38526036
J Cell Mol Med

Reduced chemokine C-C motif ligand 1 expression may negatively regulate colorectal cancer progression at liver metastatic sites. [Abstract]

2024 Apr;28(7):e18193. PMID: 38506205
FASEB J

LDHA deficiency inhibits trophoblast proliferation via the PI3K/AKT/FOXO1/CyclinD1 signaling pathway in unexplained recurrent spontaneous abortion. [Abstract]

2023 Feb;37(2):e22744. PMID: 36583693
J Cell Mol Med

Slight up-regulation of Kir2.1 channel promotes endothelial progenitor cells to transdifferentiate into a pericyte phenotype by Akt/mTOR/Snail pathway. [Abstract]

2021 Nov;25(21):10088-10100. PMID: 34592781
J Cell Mol Med

Aberrant methylation of WD-repeat protein 41 contributes to tumour progression in triple-negative breast cancer. [Abstract]

2020 Jun;24(12):6869-6882. PMID: 32394588
FASEB J

Par6 regulates cell cycle progression through enhancement of Akt/PI3K/GSK-3β signaling pathway activation in glioma. [Abstract]

2020 Jan;34(1):1481-1496. PMID: 31914615
Mediators Inflamm

Andrographolide Protects against HG-Induced Inflammation, Apoptosis, Migration, and Impairment of Angiogenesis via PI3K/AKT-eNOS Signalling in HUVECs. [Abstract]

2019 Oct 7;2019:6168340. PMID: 31686985
FEBS J

Systems analysis identifies potential target genes to overcome cetuximab resistance in colorectal cancer cells. [Abstract]

2019 Apr;286(7):1305-1318. PMID: 30719834
J Inflamm Res

Huanglian Jiedu Decoction Alleviates Myocardial Ischemia-Reperfusion Injury via AKT-Mediated Regulation of Endoplasmic Reticulum Stress. [Abstract]

2025 Dec 3:18:17025-17044. PMID: 41368345
iScience

TRIM36 inhibits lung fibroblast activation and pulmonary fibrosis through the degradation of phospho-AKT1. [Abstract]

2025 Oct 15;28(11):113775. PMID: 41323265
iScience

ATP6V0D2 drives triple-negative breast cancer progression and inhibits cisplatin sensitivity by promoting PHLPP2 lysosomal degradation. [Abstract]

2025 Oct 27;28(11):113853. PMID: 41312388
iScience

Integrative multi-omics analysis decodes HOXC9-driven malignant transformation and metastasis in OSCC. [Abstract]

2025 Sep 10;28(9):112919. PMID: 41054515
iScience

Broad and diverse roles of sphingosine-1-phosphate/sphingosine-1-phosphate receptors in the prostate. [Abstract]

2024 Oct 30;27(12):111290. PMID: 39618500
Oncol Res

LncRNA AFAP1-AS1 exhibits oncogenic characteristics and promotes gemcitabine-resistance of cervical cancer cells through miR-7-5p/EGFR axis. [Abstract]

2024 Nov 13;32(12):1867-1879. PMID: 39574469
J Lipid Res

Metallothionein 1B attenuates inflammation and hepatic steatosis in MASH by inhibiting the AKT/PI3K pathway. [Abstract]

2024 Nov 15:100701. PMID: 39551239
Transl Oncol

Unraveling the mechanisms of RECQL4-mediated cervical cancer progression through the PI3K/AKT pathway. [Abstract]

2024 Dec:50:102146. PMID: 39378549
iScience

SGMS1 facilitates osteogenic differentiation of MSCs and strengthens osteogenesis-angiogenesis coupling by modulating Cer/PP2A/Akt pathway. [Abstract]

2024 Mar 5;27(4):109358. PMID: 38544565
iScience

Trigeminal nerve-derived substance P regulates limbal stem cells by the PI3K-AKT pathway. [Abstract]

2023 Apr 18;26(5):106688. PMID: 37187698
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Schlafen 11 expression in human acute leukemia cells with gain-of-function mutations in the interferon-JAK signaling pathway. [Abstract]

2021 Sep 25;24(10):103173. PMID: 34693224
Front Mol Biosci

The Role of TRPC6 in Renal Ischemia/Reperfusion and Cellular Hypoxia/Reoxygenation Injuries. [Abstract]

2021 Jul 8:8:698975. PMID: 34307458
J Cell Physiol

BKCa channel participates in insulin-induced lipid deposition in adipocytes by increasing intracellular calcium. [Abstract]

2021 Aug;236(8):5818-5831. PMID: 33432604
Fish Shellfish Immunol

2026 Aug:175:111443. PMID: 42162881
J Biol Chem

The E3 ligase β-TRCP1 earmarks OTUD3 for destruction to fine-tune cGAS activation. [Abstract]

2026 Jun;302(6):111461. PMID: 41999888
J Biol Chem

Downregulation of ClC-3 chloride channels in dorsal root ganglia neurons contributes to bone metastasis-induced pain. [Abstract]

2026 Mar;302(3):111268. PMID: 41655695
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Involvement of AKT/β-catenin in Enhanced Osteogenesis by Cannabidiol in Bone Stromal Cells Harvested from Human Tori. [Abstract]

2026 Feb 20. PMID: 41720800
Sci Rep

Pseudomonas aeruginosa-derived DnaJ functions as a novel immunomodulator inducing IFNβ via CME-SGK1-IRF3 axis in macrophages. [Abstract]

2025 Dec 3. PMID: 41339751
Sci Rep

Panax notoginseng saponins suppress the PI3K/AKT pathway to enhance autophagy and apoptosis in pulmonary fibrosis. [Abstract]

2025 Nov 27;15(1):43622. PMID: 41309997
Sci Rep

Establishment of novel cholangiocarcinoma cell lines with ARID1A deficiency and preclinical validation of synthetic lethality therapies. [Abstract]

2025 Nov 18;15(1):40619. PMID: 41254205
Sci Rep

SREBP-2 promotes cancer progression through the mevalonate-Akt pathway in non-small cell lung cancer. [Abstract]

2025 Jul 2;15(1):23103. PMID: 40595126
Sci Rep

AKT activation triggers Rab14-mediated ADAM10 translocation to the cell surface in human aortic endothelial cells. [Abstract]

2025 Mar 3;15(1):7448. PMID: 40032916
Sci Rep

Arctiin alleviates knee osteoarthritis by suppressing chondrocyte oxidative stress induced by accumulated iron via AKT/NRF2/HO-1 signaling pathway. [Abstract]

2024 Dec 30;14(1):31935. PMID: 39738432
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Blockage of Akt activation suppresses cadmium-induced renal tubular cellular damages through aggrephagy in HK-2 cells. [Abstract]

2024 Jun 24;14(1):14552. PMID: 38914593
J Biol Chem

Nuclear factor Nrf2 promotes glycosidase OGG1 expression by activating the AKT pathway to enhance leukemia cell resistance to cytarabine. [Abstract]

2023 Jan;299(1):102798. PMID: 36528059
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Development of actionable targets of multi-kinase inhibitors (AToMI) screening platform to dissect kinase targets of staurosporines in glioblastoma cells. [Abstract]

2022 Aug 13;12(1):13796. PMID: 35963891
J Biol Chem

Prolonged deprivation of arginine or leucine induces PI3K/Akt-dependent reactivation of mTORC1. [Abstract]

2022 Jun;298(6):102030. PMID: 35577075
Aging (Albany NY)

NET1 promotes HCC growth and metastasis in vitro and in vivo via activating the Akt signaling pathway. [Abstract]

2021 Apr 11;13(7):10672-10687. PMID: 33839702
J Biomed Mater Res A

Immunomodulatory property and its regulatory mechanism of double network hydrogel on dendritic cells. [Abstract]

2021 Jun;109(6):1015-1026. PMID: 32856407
Aging

Resveratrol inhibits the malignant progression of hepatocellular carcinoma via MARCH1-induced regulation of PTEN/AKT signaling. [Abstract]

2020 Jun 12;12(12):11717-11731. PMID: 32530437
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Vitamin K2 promotes PI3K/AKT/HIF-1α-mediated glycolysis that leads to AMPK-dependent autophagic cell death in bladder cancer cells. [Abstract]

2020 May 7;10(1):7714. PMID: 32382009
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Downregulation of PDCD4 induced by progesterone is mediated by the PI3K/AKT signaling pathway in human endometrial cancer cells. [Abstract]

2019 Aug;42(2):849-856. PMID: 31233196
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E-cadherin loss induces targetable autocrine activation of growth factor signalling in lobular breast cancer. [Abstract]

2018 Oct 18;8(1):15454 PMID: 30337563
J Biol Chem

Kinome-wide selectivity profiling of ATP-competitive mammalian target of rapamycin (mTOR) inhibitors and characterization of their binding kinetics. [Abstract]

2012 Mar 23;287(13):9742-52. PMID: 22223645
PNAS Nexus

Ribosome stalling during c-myc translation presents actionable cancer cell vulnerability. [Abstract]

2024 Aug 13;3(8):pgae321. PMID: 39161732
Cell Signal

2026 Oct:146:112676. PMID: 42297063
Biochim Biophys Acta Mol Cell Res

FBXO32 activates the PI3K/AKT pathway by inhibiting PTEN through ubiquitination of TAL1 in hepatocellular carcinoma. [Abstract]

2026 Apr;1873(4):120125. PMID: 41713558
Cell Signal

Lactate induces oxidative phosphorylation in osteoblasts via Gpr81-Stat3 signaling. [Abstract]

2025 May 17:111877. PMID: 40389045
Cell Signal

STEAP4 modulates cell proliferation and oxidative stress in benign prostatic hyperplasia. [Abstract]

2024 Jan:113:110933. PMID: 37866665
Mol Cell Biochem

Inhibition of STIM1 alleviates high glucose-induced proliferation and fibrosis by inducing autophagy in mesangial cells. [Abstract]

2024 Sep;479(9):2365-2379. PMID: 37736800
Brain Res Bull

Hypocretin-1 suppresses malignant progression of glioblastoma cells through Notch1 signaling pathway. [Abstract]

2023 May:196:46-58. PMID: 36925051
Reproduction

WNT16 from decidual stromal cells regulates HTR8/SVneo trophoblastic cell function via AKT/beta-catenin pathway. [Abstract]

2022 Mar 10;163(5):241-250. PMID: 35174787
Cell Signal

Akt phosphorylation regulated by IKKε in response to low shear stress leads to endothelial inflammation via activating IRF3. [Abstract]

2021 Apr:80:109900. PMID: 33370582
Brain Res Bull

Inhibition of mTORC1 improves STZ-induced AD-like impairments in mice. [Abstract]

2020 Sep;162:166-179. PMID: 32599128
Cytokine

PI3K mediates tumor necrosis factor induced-necroptosis through initiating RIP1-RIP3-MLKL signaling pathway activation. [Abstract]

2020 May;129:155046. PMID: 32114297
Development

Axin1 regulates tooth root development by inhibiting AKT1-mTORC1 activation and Shh translation in Hertwig's epithelial root sheath. [Abstract]

2024 Nov 1;151(21):dev202899. PMID: 39344774
Heliyon

Role of HDAC3 in the epithelial-mesenchymal transition of retinal pigment epithelium cells: Implications for proliferative vitreoretinopathy. [Abstract]

2024 Oct 11;10(21):e39333. PMID: 39524785
Heliyon

PTOV1 facilitates colorectal cancer cell proliferation through activating AKT1 signaling pathway. [Abstract]

2024 Aug 10;10(16):e36017. PMID: 39229496
Biol Trace Elem Res

Ortho-silicic Acid Plays a Protective Role in Glucocorticoid-Induced Osteoporosis via the Akt/Bad Signal Pathway In Vitro and In Vivo. [Abstract]

2023 Feb;201(2):843-855. PMID: 35314965
Bone

PTX3 promotes osteogenic differentiation by triggering HA/CD44/FAK/AKT positive feedback loop in an inflammatory environment. [Abstract]

2022 Jan:154:116231. PMID: 34653679
Stem Cells

M2 Macrophages Enhance the Cementoblastic Differentiation of Periodontal Ligament Stem Cells via the Akt and JNK Pathways. [Abstract]

2019 Dec;37(12):1567-1580. PMID: 31400241
Front Med

2026 May 4:13:1828021. PMID: 42210954
Mol Med Rep

ER‑α36 knockdown is associated with lysosomal dysfunction and proliferation inhibition in liver cancer cells. [Abstract]

2025 Oct;32(4):284. PMID: 40808340
Food Chem Toxicol

Chronic exposure to polystyrene micro/nanoplastics triggers testicular dysfunction through PI3K/AKT/mTOR signaling-mediated spermatocyte senescence in mice. [Abstract]

2025 Aug 14:205:115703. PMID: 40818636
Mol Med Rep

MUC1‑ND interacts with TRPV1 to promote corneal epithelial cell proliferation in diabetic dry eye mice by partly activating the AKT signaling pathway. [Abstract]

2024 Dec;30(6):213. PMID: 39370807
Clin Exp Hypertens

FGFR2 modulates the Akt/Nrf2/ARE signaling pathway to improve angiotensin II-induced hypertension-related endothelial dysfunction. [Abstract]

2023 Dec 31;45(1):2208777. PMID: 37154169
Mol Med Rep

CMTM5 inhibits the development of prostate cancer via the EGFR/PI3K/AKT signaling pathway. [Abstract]

2022 Jan;25(1):17. PMID: 34791506
Mol Med Rep

LY‑294002 enhances the chemosensitivity of liver cancer to oxaliplatin by blocking the PI3K/AKT/HIF‑1α pathway. [Abstract]

2021 Jul;24(1):508. PMID: 33982772
Exp Cell Res

CKIP-1 augments autophagy in steatotic hepatocytes by inhibiting Akt/mTOR signal pathway. [Abstract]

2020 Dec 1;397(1):112341. PMID: 33191205
Food Chem Toxicol

Diallyl sulfide protects against lipopolysaccharide/d-galactosamine-induced acute liver injury by inhibiting oxidative stress, inflammation and apoptosis in mice. [Abstract]

2018 Oct:120:500-509. PMID: 30075314
J Diabetes Res

Targeting Oxidative Stress and Apoptosis via PI3K/Akt/Nrf2 Pathway: The Therapeutic Role of Bletilla striata Polysaccharide in Diabetic Wound Repair. [Abstract]

2026 Jan 28:2026:5751331. PMID: 41613340
BMC Cancer

Aldo-keto reductase family 1 member C3 mediates radioresistance of esophageal cancer cells through suppressing MAPK and AKT signaling. [Abstract]

2024 Oct 7;24(1):1236. PMID: 39375680
J Hepatocell Carcinoma

ZDHHC20 Activates AKT Signaling Pathway to Promote Cell Proliferation in Hepatocellular Carcinoma. [Abstract]

2024 Sep 18:11:1763-1775. PMID: 39309302
BMC Complement Med Ther

Astragalus polysaccharide restores insulin secretion impaired by lipopolysaccharides through the protein kinase B /mammalian target of rapamycin/glucose transporter 2 pathway. [Abstract]

2023 Oct 10;23(1):358. PMID: 37817130
Cell Cycle

Cancer-associated fibroblast-released extracellular vesicles carrying miR-199a-5p induces the progression of gastric cancer through regulation of FKBP5-mediated AKT1/mTORC1 signaling pathway. [Abstract]

2022 Dec;21(24):2590-2601. PMID: 36005478
Toxicol Appl Pharmacol

Icariin promotes mouse Leydig cell testosterone synthesis via the Esr1/Src/Akt/Creb/Sf-1 pathway. [Abstract]

2022 Apr 15:441:115969. PMID: 35259346
Bosn J Basic Med Sci

Stomach-specific c-Myc overexpression drives gastric adenoma in mice through AKT/mammalian target of rapamycin signaling. [Abstract]

2021 Aug 1;21(4):434-446. PMID: 33259779
J Immunol

AKT Regulates NLRP3 Inflammasome Activation by Phosphorylating NLRP3 Serine 5. [Abstract]

2020 Oct 15;205(8):2255-2264. PMID: 32929041
Chem Biol Drug Des

Exploring the Therapeutic Potential of Oridonin in the Treatment of Laryngeal Cancer: A Comprehensive Strategy Involving Network Pharmacology, Molecular Docking, Dynamic Simulation, and Experimental Verification. [Abstract]

2026 Jan;107(1):e70222. PMID: 41525139
Chem Biol Drug Des

Therapeutic Potential of 6-Gingerol in Glioma: Mechanistic Insights and Experimental Validation. [Abstract]

2025 Dec;106(6):e70217. PMID: 41351348
Am J Physiol Gastrointest Liver Physiol

Plant green pigment of chlorophyllin attenuates inflammatory bowel diseases by suppressing autophagy activation in mice. [Abstract]

2022 Aug 1;323(2):G102-G113. PMID: 35638642
Mol Carcinog

ZNF480 Accelerates Chemotherapy Resistance in Breast Cancer by Competing With TRIM28 and Stabilizing LSD1 to Upregulate the AKT-GSK3β-Snail Pathway. [Abstract]

2025 Jan;64(1):192-208. PMID: 39503216
Int J Med Sci

Enhanced Bone Regeneration by Schwann Cells through Coupling of Osteogenesis and Angiogenesis via β-catenin signaling in a Preclinical Model of Distraction Osteogenesis. [Abstract]

2025 Jan 1;22(1):209-226. PMID: 39744173
Mol Carcinog

LAIR1 promotes hepatocellular carcinoma cell metastasis and induces M2-macrophage infiltration through activating AKT-IKKβ-p65 axis. [Abstract]

2024 Sep;63(9):1827-1841. PMID: 39016636
Cell Stress Chaperones

Roles of heat shock protein A12A in the development of diabetic cardiomyopathy. [Abstract]

2024 Apr;29(2):272-284. PMID: 38485044
J Pharm Pharmacol

Ethyl 2,2-difluoro-2-(2-oxo-2H-chromen-3-yl) acetate attenuates the malignant biological behaviors of non-small cell lung cancer via suppressing EGFR/PI3K/AKT/mTOR signaling pathway. [Abstract]

2024 Mar 4;76(3):269-282. PMID: 38241189
Environ Toxicol

MUC1 promotes lymph node metastasis in esophageal squamous cell carcinoma by downregulating DNAJB6 expression. [Abstract]

2024 Jan;39(1):9-22. PMID: 37584547
Cytotherapy

Mesenchymal stromal cells regulate THP-1-differentiated macrophage cytokine production by activating Akt/mammalian target of rapamycin complex 1 pathway. [Abstract]

2023 Aug;25(8):858-865. PMID: 37125989
Mol Carcinog

LATS-regulated nuclear-cytoplasmic translocation of SREBP2 inhibits hepatocellular carcinoma cell migration and invasion via epithelial-mesenchymal transition. [Abstract]

2023 Jul;62(7):963-974. PMID: 37042569
J Cancer

Role of DEP domain-containing protein 1B (DEPDC1B) in epithelial ovarian cancer. [Abstract]

2023 Mar 27;14(5):784-792. PMID: 37056386
J Cancer

Cry 1 Regulates the Clock Gene Network and Promotes Proliferation and Migration Via the Akt/P53/P21 Pathway in Human Osteosarcoma Cells. [Abstract]

2018 Jun 14;9(14):2480-2491. PMID: 30026846

MK-2206 free base purchased from MedChemExpress. Usage Cited in: J Cancer. 2018 Jun 14;9(14):2480-2491. [Abstract]

Analysis of indicated proteins in Cry1 silencing osteosarcoma cells after MK-2206 treatment. Data are expressed as the mean±standard deviation.
Hum Mol Genet

BMP4 uses several different effector pathways to regulate proliferation and differentiation in the epithelial and mesenchymal tissue compartments of the developing mouse ureter. [Abstract]

2017 Sep 15;26(18):3553-3563. PMID: 28655168

MK-2206 free base purchased from MedChemExpress. Usage Cited in: Hum Mol Genet. 2017 Sep 15;26(18):3553-3563. [Abstract]

Immunofluorescence analysis for expression of the I-cell marker ΔNP63 on proximal sections of ureters explanted from E12.5 wildtype (control) embryos and cultured for 6 d in the presence of solvent (DMSO) (A), the AKT inhibitor (AKT-i) MK2206 (B), the P38 inhibitor (P38-i) SB203580 (C), the ERK1/2 inhibitor (ERK1/2-i) PD98059 (D) or combinations as indicated (E and F).
Naunyn Schmiedebergs Arch Pharmacol

Medicarpin reduces cisplatin resistance and causes apoptosis by inhibiting the AKT/Bcl2 pathway in breast cancer. [Abstract]

2025 Oct 24. PMID: 41134353
Cancer Med

Focal Adhesion Kinase Intersects With the BRD4-MYC Axis and YAP1 to Drive Tumor Cell Growth, Phenotypic Plasticity, Stemness, and Metastatic Potential in Colorectal Cancer. [Abstract]

2025 Sep;14(18):e71227. PMID: 40959971
J Diabetes Complications

Modulation of the sodium-chloride cotransporter by insulin in auditory cells: A potential link to diabetes-related hearing complications. [Abstract]

2025 Jun 22;39(9):109111. PMID: 40561788
Curr Issues Mol Biol

The Electric Field Guided HaCaT Cell Migration Through the EGFR/p38 MAPK/Akt Pathway. [Abstract]

2024 Dec 31;47(1):16. PMID: 39852131
Arch Biochem Biophys

Carboxypeptidase A6 suppresses the proliferation and invasion of colorectal cancer cells and is negatively regulated by miR-96-3p. [Abstract]

2023 May 15:740:109595. PMID: 37011707
Mol Pharmacol

Functional RNAi Screens Define Distinct Protein Kinase Vulnerabilities in EGFR-Dependent HNSCC Cell Lines. [Abstract]

2019 Dec;96(6):862-870. PMID: 31554698
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ATAXIA TELANGIECTASIA MUTATED PROTECTS AGAINST LIPOPOLYSACCARIDE-INDUCED BLOOD-BRAIN BARRIER DISRUPTION BY REGULATING ATK/DRP1-MEDIATED MITOCHONDRIAL HOMEOSTASIS. [Abstract]

2023 Jul 1;60(1):100-109. PMID: 37141173
J Reprod Immunol

4-vinylcyclohexene diepoxide induces premature ovarian insufficiency in rats by triggering the autophagy of granule cells through regulating miR-144. [Abstract]

2023 Jun:157:103928. PMID: 36889083
Life Sci Alliance

ERK-Smurf1-RhoA signaling is critical for TGFβ-drived EMT and tumor metastasis. [Abstract]

2022 Jun 2;5(10):e202101330. PMID: 35654587
Cell Immunol

CD36 regulates LPS-induced acute lung injury by promoting macrophages M1 polarization. [Abstract]

2022 Feb:372:104475. PMID: 35063904
Front Cardiovasc Med

TANK Promotes Pressure Overload Induced Cardiac Hypertrophy via Activating AKT Signaling Pathway. [Abstract]

2021 Sep 3;8:687540. PMID: 34540911
Am J Cancer Res

TM2D1 contributes the epithelial-mesenchymal transition of hepatocellular carcinoma via modulating AKT/β-catenin axis. [Abstract]

2021 Apr 15;11(4):1557-1571. PMID: 33948373
R Soc Open Sci

Ultra-performance liquid chromatography-tandem mass spectrometric method for quantitation of the recently Food and Drug Administration approved combination of vaborbactam and meropenem in human plasma. [Abstract]

2020 Jul 8;7(7):200635. PMID: 32874656
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RFamide-related peptide-3 promotes alpha TC1 clone 6 cell survival likely via GPR147. [Abstract]

2018 Sep:107:39-44. PMID: 30081043
Int J Biochem Cell Biol

Bad is essential for Bcl-xL-enhanced Bax shuttling between mitochondria and cytosol. [Abstract]

2023 Feb:155:106359. PMID: 36586532
Mol Biol Rep

Combined treatment with ruxolitinib and MK-2206 inhibits the JAK2/STAT5 and PI3K/AKT pathways via apoptosis in MDA-MB-231 breast cancer cell line. [Abstract]

2023 Jan;50(1):319-329. PMID: 36331743
Mol Biol Rep

mTOR inhibitor PP242 increases antitumor activity of sulforaphane by blocking Akt/mTOR pathway in esophageal squamous cell carcinoma. [Abstract]

2022 Jan;49(1):451-461. PMID: 34731371
J Biochem Mol Toxicol

ATGL promotes the proliferation of hepatocellular carcinoma cells via the p-AKT signaling pathway. [Abstract]

2019 Nov;33(11):e22391. PMID: 31476254
J Cell Biochem

Absent in melanoma 2 suppresses epithelial-mesenchymal transition via Akt and inflammasome pathways in human colorectal cancer cells. [Abstract]

2019 Oct;120(10):17744-17756. PMID: 31210372
Int J Biochem Cell Biol

NDRG2 suppresses proliferation, migration, invasion and epithelial-mesenchymal transition of esophageal cancer cells through regulating the AKT/XIAP signaling pathway. [Abstract]

2018 Jun:99:43-51. PMID: 29530788

MK-2206 free base purchased from MedChemExpress. Usage Cited in: Int J Biochem Cell Biol. 2018 Jun:99:43-51. [Abstract]

Western blot analysis of NDRG2, p-ATK, XIAP, E-cadherin and Vimentin in TE-13 cells shows NDRG2 negatively regulates the expressions of AKT, XIAP, E-cadherin and Vimentin proteins.
J Cell Biochem

Matrine reversed multidrug resistance of breast cancer MCF-7/ADR cells through PI3K/AKT signaling pathway. [Abstract]

2018 May;119(5):3885-3891. PMID: 29130495

MK-2206 free base purchased from MedChemExpress. Usage Cited in: J Cell Biochem. 2018 May;119(5):3885-3891. [Abstract]

Western blot showed the protein expression of P-gp,MRP1, PTEN, p-AKT, Bax, Bcl-2, and cleaved caspase-3.
Int J Biochem Cell Biol

STYK1 promotes cancer cell proliferation and malignant transformation by activating PI3K-AKT pathway in gallbladder carcinoma. [Abstract]

2018 Apr:97:16-27. PMID: 29413947
Vet Microbiol

The Chinese medicine monomer Schisandrin C inhibits PRRSV infection by regulating the OGT-PI3K/AKT/mTOR signaling pathway. [Abstract]

2026 May:316:110992. PMID: 41865607
Microbes Infect

Pseudomonas aeruginosa-derived DnaJ induces TLR2 expression through TLR10-mediated activation of the PI3K-SGK1 pathway in macrophages. [Abstract]

2025 May-Jun;27(4):105481. PMID: 39978578
Microvasc Res

GBP2 inhibits pathological angiogenesis in the retina via the AKT/mTOR/VEGFA axis. [Abstract]

2024 Jul:154:104689. PMID: 38636926
J Integr Neurosci

Heliox Protects SH-SY5Y Cells from Oxygen-Glucose Deprivation/Reperfusion-Induced Ferroptosis. [Abstract]

2024 Jan 16;23(1):14. PMID: 38287843
Toxicol In Vitro

Everolimus prevents doxorubicin-induced apoptosis in H9c2 cardiomyocytes but not in MCF-7 cancer cells: Cardioprotective roles of autophagy, mitophagy, and AKT. [Abstract]

2023 Dec:93:105698. PMID: 37739323
Radiat Res

Mitochondrial Dysfunctions Regulated Radioresistance through Mitochondria-to-Nucleus Retrograde Signaling Pathway of NF-κB/PI3K/AKT2/mTOR. [Abstract]

2018 Aug;190(2):204-215. PMID: 29863983

MK-2206 free base purchased from MedChemExpress. Usage Cited in: Radiat Res. 2018 Aug;190(2):204-215. [Abstract]

When 6 Gy irradiation is combined with AKT2 inhibitor MK-2206 treatment, the protein levels in phosphorylated AKT2, mTOR and IKBa are decreased, and the downstream proapoptotic proteins, caspase 3 and caspase 8, are increased.
Exp Eye Res

Expression and regulation of alarmin cytokine IL-1α in human retinal pigment epithelial cells. [Abstract]

2018 Jul:172:10-20. PMID: 29551335
PLoS One

Exosomes from human umbilical cord mesenchymal stem cells promote the growth of human hair dermal papilla cells. [Abstract]

2025 Apr 30;20(4):e0320154. PMID: 40305498
Platelets

Alantolactone induces platelet apoptosis by activating the Akt pathway. [Abstract]

2023 Dec;34(1):2173505. PMID: 36813739
PLoS One

Targeting the mTOR Complex by Everolimus in NRAS Mutant Neuroblastoma. [Abstract]

2016 Jan 28;11(1):e0147682. PMID: 26821351
Pulm Circ

2026 Apr 23;16(2):e70306. PMID: 42039681
Chem Biodivers

Total Flavonoids of Hovenia dulcis Seeds Ameliorate Hepatic Fibrosis via Modulating PI3K/AKT Signaling Pathway In Vitro and In Vivo. [Abstract]

2025 Oct 24:e02088. PMID: 41135015
Tissue Cell

Nanosphere loaded with curcumin attenuates adipogenesis and lipid metabolism by modulating AKT/mTORC1/PPARγ phosphorylation. [Abstract]

2025 Aug 29:97:103112. PMID: 40886657
Clin Exp Pharmacol Physiol

Dapagliflozin alleviates high glucose-induced injury of endothelial cells via inducing autophagy. [Abstract]

2024 Apr;51(4):e13846. PMID: 38382536
J Pain Res

Intravenous Administration of Triptonide Attenuates CFA-Induced Pain Hypersensitivity by Inhibiting DRG AKT Signaling Pathway in Mice. [Abstract]

2020 Dec 1;13:3195-3206. PMID: 33293856
Clin Exp Pharmacol Physiol

SPNS2 promotes the malignancy of colorectal cancer cells via regulating Akt and ERK pathway. [Abstract]

2019 Sep;46(9):861-871. PMID: 31206801
Medicina (Kaunas)

AKT Signaling Regulates Agrin-Mediated Acetylcholine Receptor Surface Density. [Abstract]

2026 Feb 27;62(3):456. PMID: 41901538
Virology

The Pan-ErbB tyrosine kinase inhibitor afatinib inhibits multiple steps of the mammarenavirus life cycle. [Abstract]

2022 Nov:576:83-95. PMID: 36183499
PeerJ

Icariside II induces rapid phosphorylation of endothelial nitric oxide synthase via multiple signaling pathways. [Abstract]

2022 Oct 25:10:e14192. PMID: 36312762
Int J Endocrinol

Umbilical Cord-Derived Mesenchymal Stem Cells Improve Ornidazole-Induced Asthenozoospermia in Rats via Activation of the AKT/mTOR Pathway. [Abstract]

2024 Nov 12:2024:3494652. PMID: 39564353
Thorac Cancer

ACT001 inhibits the proliferation of non-small cell lung cancer cells by upregulating NKTR expression. [Abstract]

2022 Jun;13(12):1772-1782. PMID: 35537816
Exp Ther Med

mTORC1 is a key regulator that mediates OGD- and TGFβ1-induced myofibroblast transformation and chondroitin-4-sulfate expression in cardiac fibroblasts. [Abstract]

2022 Jun;23(6):413. PMID: 35601064
Exp Ther Med

Assessing the anti-inflammatory effects of quercetin using network pharmacology and in vitro experiments. [Abstract]

2022 Apr;23(4):301. PMID: 35340883
Exp Ther Med

Benazepril hydrochloride protects against doxorubicin cardiotoxicity by regulating the PI3K/Akt pathway. [Abstract]

2021 Oct;22(4):1082. PMID: 34447475
FEBS Open Bio

METTL3 mediates bone marrow mesenchymal stem cell adipogenesis to promote chemoresistance in acute myeloid leukaemia. [Abstract]

2021 Jun;11(6):1659-1672. PMID: 33932138
Exp Ther Med

miRNA-214 suppresses oxidative stress in diabetic nephropathy via the ROS/Akt/mTOR signaling pathway and uncoupling protein 2. [Abstract]

2019 May;17(5):3530-3538. PMID: 30988734
Biochem Biophys Res Commun

HYAL2-generated low-molecular-weight hyaluronic acid promotes intervertebral disc degeneration via the CD44/AKT signaling axis. [Abstract]

2026 Apr 30:811:153576. PMID: 41806573
Biochem Biophys Res Commun

Flavonoid derivative FB-15 inhibits the progression of nasopharyngeal carcinoma by targeting the PI3K-Akt signaling pathway. [Abstract]

2025 May 16:771:152037. PMID: 40409114
Biochem Biophys Res Commun

STING1 targets MYH9 to drive adipogenesis through the AKT/GSK3β/β-catenin pathway. [Abstract]

2025 Jan 16:749:151352. PMID: 39847995
Leuk Res

PI3K/AKT confers intrinsic and acquired resistance to pirtobrutinib in chronic lymphocytic leukemia. [Abstract]

2024 Jul 7:144:107548. PMID: 39018782
Biochem Biophys Res Commun

PCK2 induces gefitinib resistance by suppresses ferroptosis in non-small cell lung cancer. [Abstract]

2024 Jun 1:723:150200. PMID: 38850814
Oncol Lett

EGFR mediates epithelial‑mesenchymal transition through the Akt/GSK-3β/Snail signaling pathway to promote liver cancer proliferation and migration. [Abstract]

2023 Dec 18;27(2):59. PMID: 38192662
Biochem Biophys Res Commun

Engrailed 2 triggers the activation of multiple phosphorylation-induced signaling pathways in both transcription-dependent and -independent manners. [Abstract]

2023 Nov 5:680:127-134. PMID: 37738902
Biochem Biophys Res Commun

Monotropein attenuates doxorubicin-induced oxidative stress, inflammation, and arrhythmia via the AKT signal pathway. [Abstract]

2023 Jan 1:638:14-22. PMID: 36436337
Biochem Biophys Res Commun

FGF5 alleviated acute lung injury via AKT signal pathway in endothelial cells. [Abstract]

2022 Dec 17:634:152-158. PMID: 36244113
Biochem Biophys Res Commun

CaMKII and Kalirin, a Rac1-GEF, regulate Akt phosphorylation involved in contraction-induced glucose uptake in skeletal muscle cells. [Abstract]

2022 Jun 25;610:170-175. PMID: 35462099
Biochem Biophys Res Commun

GIP-GIPR promotes neurite outgrowth of cortical neurons in Akt dependent manner. [Abstract]

2021 Jan 1:534:121-127. PMID: 33321289
Oncol Lett

7-dehydrocholesterol suppresses melanoma cell proliferation and invasion via Akt1/NF-κB signaling. [Abstract]

2020 Dec;20(6):398. PMID: 33193858
J Mol Histol

Akt plays indispensable roles during the first cell lineage differentiation of mouse. [Abstract]

2019 Aug;50(4):369-374. PMID: 31190160
Biochem Biophys Res Commun

SRPIN340 protects heart muscle from oxidative damage via SRPK1/2 inhibition-mediated AKT activation. [Abstract]

2019 Feb 26;510(1):97-103. PMID: 30661787
Sex Med

Hinokiflavone alleviates high-fat diet-induced erectile dysfunction via the EGFR/PI3K/Akt/eNOS signaling pathway. [Abstract]

2025 Aug 6;13(4):qfaf059. PMID: 40808867
Cytotechnology

Lysine-proline-valine peptide attenuates hepatic lipid accumulation through ROS-dependent regulation of the PPARγ pathway in HepG2 cells. [Abstract]

2026 Jun;78(3):98. PMID: 42064835
Cytotechnology

β-sitosterol in Yijing Hugui decoction prevents cyclophosphamide-induced premature ovarian insufficiency via the AKT1/Nrf2 pathway. [Abstract]

2025 Apr;77(2):76. PMID: 40078376
J Biochem

Ciliary length variations impact cilia-mediated signaling and biological responses. [Abstract]

2024 Aug 8:mvae057. PMID: 39115281
Cell Reprogram

Comparison of the Cytokine Profile in Mesenchymal Stem Cells from Human Adipose, Umbilical Cord, and Placental Tissues. [Abstract]

2021 Dec;23(6):336-348. PMID: 34677101
In Vitro Cell Dev Biol Anim

MicroRNA-7a inhibits Isl1 expression to regulate insulin secretion by targeting Raf1 and Mapkap1 in NIT-1 cells. [Abstract]

2021 Sep;57(8):817-824. PMID: 34713362
Hematology

AKT inhibition sensitizes acute leukemia cells to S63845-induced apoptosis. [Abstract]

2023 Dec;28(1):2214465. PMID: 37222135
Curr Med Sci

Celastrol Induces Apoptosis and Autophagy via the AKT/mTOR Signaling Pathway in the Pituitary ACTH-secreting Adenoma Cells. [Abstract]

2022 Apr;42(2):387-396. PMID: 35419676
Brain Dev

RCOR1 improves neurobehaviors and neuron injury in rat cerebral palsy by Endothelin-1 targeting-induced Akt/GSK-3β pathway upregulation. [Abstract]

2024 Feb;46(2):93-102. PMID: 37978036
Acta Otolaryngol

Insulin regulates Nedd4-2 via a PKB-dependent mechanism in HEI-OC1 auditory cells-crosstalks with sphingolipid and cAMP signaling. [Abstract]

2022 Jan;142(1):6-12. PMID: 34962430
Dev Growth Differ

Inhibition of phosphorylated Ser473-Akt from translocating into the nucleus contributes to 2-cell arrest and defective zygotic genome activation in mouse preimplantation embryogenesis. [Abstract]

2016 Apr;58(3):280-92. PMID: 27004788
Int J Clin Exp Pathol

2017;10(3):3033-3042.
Int J Clin Exp Med

2016;9(9):18513-18518.
SSRN

2026 Apr 28.
bioRxiv

ATM functions as a rheostat of metabolic stress in small-cell lung cancer. [Abstract]

2026 Mar 17:2026.03.13.711672. PMID: 41890037
bioRxiv

2025 Nov 3.
SSRN

2025 Oct 29.
SSRN

2025 Nov 5.
University of Otago

2025 Oct 7.
University of Colorado

2025.
SSRN

2025 Aug 6.
Mechanobiol Med

Piezo1 activates nitric oxide synthase in red blood cells via protein kinase C with increased activity in diabetes. [Abstract]

2025 Jul 28;3(3):100145. PMID: 40809083
Cornell University

2025
bioRxiv

2025 March 20.
bioRxiv

2025 March 03.
bioRxiv

Metabolic reprogramming in the spinal cord drives the transition to pain chronicity. [Abstract]

2025 Feb 1:2025.01.30.635746. PMID: 39975205
SSRN

2024
bioRxiv

2024 August 26.
bioRxiv

2020 Jul 15.
Patent

US20210236501A1.
bioRxiv

2024 June 03.
Research Square Preprint

2024 Mar 28.
bioRxiv

2024 Feb 8.
Research Square Preprint

2023 Oct 11.
Ruperto Carola University Heidelberg

2023 Jun 1.
Research Square Preprint

2023 May 23.
Research Square Preprint

2023 May 3.
bioRxiv

2023 May 14.
Research Square Preprint

2023 May 5.
Research Square Print

2023 Mar 9.
Indian J Pharm Sci

2022.
Oxid Med Cell Longev

Hypoxia Enhances Glioma Resistance to Sulfasalazine-Induced Ferroptosis by Upregulating SLC7A11 via PI3K/AKT/HIF-1 α Axis. [Abstract]

2022 Nov 18:2022:7862430. PMID: 36439690
Research Square Print

October 11th, 2022.
Patent

US20220288067A1.
Research Square Print

August 22nd, 2022.
Research Square Preprint

2022 Feb.
University of Zürich

2021 Dec.
Research Square Preprint

2021 Aug.
Ann Transl Med

ESM1 promotes triple-negative breast cancer cell proliferation through activating AKT/NF-κB/Cyclin D1 pathway. [Abstract]

2021 Apr;9(7):533. PMID: 33987231
Research Square Preprint

2020 Nov.
bioRxiv

2020 Mar.
Radboud University Nijmegen

2019 Oct.
Patent

US20190248778A1.
Oncotarget

αMSH inhibits adipose inflammation via reducing FoxOs transcription and blocking Akt/JNK pathway in mice. [Abstract]

2017 Jul 18;8(29):47642-47654. PMID: 28514752

MK-2206 free base purchased from MedChemExpress. Usage Cited in: Oncotarget. 2017 Jul 18;8(29):47642-47654. [Abstract]

Akt/JNK signal pathway is impaired in the inhibition of αMSH on adipocyte inflammation and FoxOs expressions. Mouse adipocytes are pretreated with αMSH and MK-2206, respectively. Relative protein levels of Akt, p-Akt^ser473, JNK, p-JNK^Thr183 with or without MK-2206 (n=3).
Oncotarget

The angiotensin II type 1 receptor antagonist telmisartan inhibits cell proliferation and tumor growth of esophageal adenocarcinoma via the AMPKα/mTOR pathway in vitro and in vivo. [Abstract]

2017 Jan 31;8(5):8536-8549. PMID: 28052030

MK-2206 free base purchased from MedChemExpress. Usage Cited in: Oncotarget. 2017 Jan 31;8(5):8536-8549. [Abstract]

Western blot analysis of cyclin D1 and cyclin E in OE19 cells treated with the control, BIBR 277 alone, MK-2206 alone, or BIBR 277 combined with MK-2206 for 48 h.
Modern Oncology

2017,25(01):0009-0013.

MK-2206 free base purchased from MedChemExpress. Usage Cited in: Modern Oncology. 2017,25(01):0009-0013.

The related protein expression of Bcl-2, Bax, Caspase-3, PARP, GSK-3β, p65 gene in each intervention group. Western blot assay:1:Control group; 2:MK2206 positive group; 3:0.3g/L Mat; 4: 0.6g/L Mat; 5:1.2g/LMat.
Patent

US20160368910A1.
Harvard University

2016 Aug.

MK-2206 free base purchased from MedChemExpress. Usage Cited in: Harvard University. 2016 Aug.

Mouse embryonic fibroblasts (MEFs) are deprived of Arginine and treated with 2 µM MK2206 for up to 6 hours.
Oncotarget

Dual inhibition of AKT/FLT3-ITD by A674563 overcomes FLT3 ligand-induced drug resistance in FLT3-ITD positive AML. [Abstract]

2016 May 17;7(20):29131-42. PMID: 27074558
Okayama University

2015.

MK-2206 free base purchased from MedChemExpress. Usage Cited in: Okayama University. 2015.

Expression of Sema4D and PlexinB1 in various oral squamous cell carcinoma cell lines following the addition of IGF-I. Notably, the addition of IGF-I resulted in increased expression of Sema4D, particularly in HSC-2 and SAS cells. This increase was suppressed when IGF-I was administered in the presence of inhibitors targeting its receptor or downstream signaling pathways. No changes in PlexinB1 expression were observed following the addition of IGF-I.
Patent

US20140309249A1.
Chem Biol

Discovery of potent and selective covalent inhibitors of JNK. [Abstract]

2012 Jan 27;19(1):140-54. PMID: 22284361

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (299 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Yan L. Abstract# DDT01-1: MK-2206: A potent oral allosteric AKT inhibitor. Cancer research. 2009 May 1;69(9_Supplement):DDT01-1.

[2]. Lu JW, et al. MK-2206 induces apoptosis of AML cells and enhances the cytotoxicity of cytarabine. Med Oncol. 2015;32(7):206. [Content Brief]

[3]. Chen M, et al. MK-2206 Alleviates Renal Fibrosis by Suppressing the Akt/mTOR Signaling Pathway In Vivo and In Vitro. Cells. 2022;11(21):3505. Published 2022 Nov 5. [Content Brief]

[4]. Bjune K, et al. MK-2206, an allosteric inhibitor of AKT, stimulates LDLR expression and LDL uptake: A potential hypocholesterolemic agent. Atherosclerosis. 2018;276:28-38. [Content Brief]

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How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

MK-2206 free base

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MK-2206 free base

High-Throughput Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) MK-2206 free base

All Akt Isoforms

All mTOR Isoforms

All GSK-3 Isoforms

All Bcl-2 Family Isoforms

Biological Activity

Chemical Information

Publications (483)

Purity & Documentation

References

Calculators

Help & FAQs

MK-2206 free base

High-Throughput Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) MK-2206 free base

All Akt Isoforms

All mTOR Isoforms

All GSK-3 Isoforms

All Bcl-2 Family Isoforms

Biological Activity

Chemical Information

Publications (483)

Purity & Documentation

References

Calculators

Help & FAQs

MK-2206 free base Related Classifications

HY-10357 Related Classifications