Cat. No.: HY-N0912

Rehmannioside D

2 Cited Publications

Customer Review

Based on 2 publication(s) in Google Scholar

Rehmannioside D is an orally active Sirt7 modulator. Rehmannioside D upregulates Sirt7 expression, inhibits the level of acetylated p53, and blocks the activation of the p53 signaling pathway. Rehmannioside D alleviates liver injury, inflammatory response, collagen deposition and hepatocyte apoptosis. Rehmannioside D is applicable to research related to liver fibrosis.

For research use only. We do not sell to patients.

Purity: 99.89%
CAS No.: 81720-08-3
Formula: C₂₇H₄₂O₂₀
Molecular Weight:686.61

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

Rehmannioside D Chemical Structure

CAS No.: 81720-08-3

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
- In Vivo
Chemical Information
Publications (2)
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

All Sirtuin Isoforms :

All Bcl-2 Family Isoforms :

All Caspase Isoforms :

In Vitro

Rehmannioside D (5-10 μM; 24 h) alleviates H₂O₂-induced apoptosis of AML12 cells by upregulating Sirt7 expression^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Apoptosis Analysis^[1]

Cell Line:

H₂O₂-injured AML12 hepatocytes
Concentration:

5 μM; 10 μM
Incubation Time:

24 h (co-incubation with H₂O₂)
Result:

Significantly reduced the proportion of apoptotic AML12 cells induced by H₂O₂, with both 5 μM and 10 μM doses yielding statistically significant effects.
Increased nuclear Sirt7 protein expression, decreased nuclear acetyl-p53 levels, reduced the Bax/Bcl2 ratio, and lowered Cleaved-caspase 3 levels compared to H₂O₂-only treated cells.

In Vivo

Rehmannioside D (10-40 mg/kg; i.g.; daily; 3 weeks) attenuates CCl₄-induced liver fibrosis in male C57BL/6J mice^[1].
Rehmannioside D (10-40 mg/kg; p.o.; daily; 2 weeks) attenuates DMN-induced liver fibrosis in male Wistar rats by upregulating Sirt7 expression, inhibiting acetyl-p53-mediated hepatocyte apoptosis, reducing hepatic injury, inflammation, and collagen deposition^[1].
Rehmannioside D (19-76 mg/kg; i.g.; daily; 2 weeks) has an ameliorative effect in a rat model of impaired ovarian reserve^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

C57BL/6J (male, 22-24 g, CCl₄-induced liver fibrosis model)^[1]
Dosage:

10 mg/kg; 40 mg/kg
Administration:

i.g.; daily; 3 weeks
Result:

Significantly reduced serum ALT and AST activities compared to CCl₄-induced controls.
Ameliorated liver histopathological damage, including reduced lobule structural distortion, hepatocellular swelling/necrosis, and inflammatory cell infiltration.
Decreased F4/80-positive macrophage area and downregulated hepatic mRNA expression of inflammatory genes (Adgre1, Tnfα, Il1β, Ccl2) compared to CCl₄-induced controls.
Reduced collagen deposition.
Reduced the number of TUNEL⁺/HNF4α⁺ hepatocytes compared to CCl₄-induced controls.
Restored hepatic Sirt7 expression, reduced nuclear acetyl-p53 levels, lowered the Bax/Bcl2 ratio, and decreased Cleaved-caspase 3 levels compared to CCl₄-induced controls.

Animal Model:

SD rats (female, 8 weeks old, Cyclophosphamide (HY-17420) induced DOR)^[3]
Dosage:

19 mg/kg, 38 mg/kg, 76 mg/kg
Administration:

i.g.; daily; 2 weeks
Result:

Notably recovered disordered estrous cycles and increased ovarian index of DOR rats.
Elevated the quantity of primordial and mature follicles while reducing atretic follicle count via ovarian histomorphology observation.
Downregulated abnormally high FSH and LH concentrations and upregulated declined E2 level.
Decreased granulosa cell apoptotic rate and upregulated FOXO1 expression.

Chemical Information

CAS No. 81720-08-3
Appearance Solid
Molecular Weight 686.61
Formula C₂₇H₄₂O₂₀
Color White to off-white
SMILES

O[C@H]1[C@@]([C@](C(CO)=C1)([H])[C@@H]2O[C@]([C@@H]([C@@H](O)[C@@H]3O)O)([H])O[C@@H]3CO)(C=CO2)O[C@@](O[C@H](CO)[C@@H](O)[C@@H]4O)([H])[C@@H]4O[C@]([C@@H]([C@@H](O)[C@@H]5O)O)([H])O[C@@H]5CO
Structure Classification
- Terpenoids
- Iridoids
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (2)

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Phytomedicine

Rehmannioside D prevents estrogen-deficiency induced osteoporosis by interacting with c-Jun to dismantle the AP-1 complex and suppress MAPK/NF-κB signaling. [Abstract]

2026 Aug:158:158371. PMID: 42251792
J Ethnopharmacol

Protecting the brain from stroke: Huoxue Rongluo formula (HXRLF) targets ferroptosis for neuroprotection. [Abstract]

2025 Jul 29;353(Pt A):120329. PMID: 40744419

Solvent & Solubility

In Vitro:

H₂O : 100 mg/mL (145.64 mM; Need ultrasonic)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.4564 mL

7.2822 mL

14.5643 mL

5 mM

0.2913 mL

1.4564 mL

2.9129 mL

10 mM

0.1456 mL

0.7282 mL

1.4564 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (287 KB)
SDS (392 KB)
- English - EN (392 KB)
- Français - FR (392 KB)
- Deutsch - DE (392 KB)
- Norwegian - NO (392 KB)
- Español - ES (392 KB)
- Swedish - SV (392 KB)
- Italian - IT (392 KB)
- Korean - KR (392 KB)
- Portuguese - PT (392 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Liang Y, et al. Rehmannioside D ameliorates hepatocyte apoptosis and liver fibrosis by suppressing Sirt7/p53 axis. J Ethnopharmacol. 2026;357:120863. [Content Brief]

[2]. Liu W, et al. Phytochemical Profiles and Antioxidant Activity of Rehmannia glutinosa from Different Production Locations. Chem Biodivers. 2020;17(8):e2000341. [Content Brief]

[3]. Liang Y, et al. Rehmannioside D mitigates disease progression in rats with experimental-induced diminished ovarian reserve via Forkhead Box O1/KLOTHO axis. The Korean Journal of Physiology & Pharmacology: Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology. 2023 Mar 1;27(2):167-76.

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O	1 mM	1.4564 mL	7.2822 mL	14.5643 mL	36.4108 mL
	5 mM	0.2913 mL	1.4564 mL	2.9129 mL	7.2822 mL
	10 mM	0.1456 mL	0.7282 mL	1.4564 mL	3.6411 mL
	15 mM	0.0971 mL	0.4855 mL	0.9710 mL	2.4274 mL
	20 mM	0.0728 mL	0.3641 mL	0.7282 mL	1.8205 mL
	25 mM	0.0583 mL	0.2913 mL	0.5826 mL	1.4564 mL
	30 mM	0.0485 mL	0.2427 mL	0.4855 mL	1.2137 mL
	40 mM	0.0364 mL	0.1821 mL	0.3641 mL	0.9103 mL
	50 mM	0.0291 mL	0.1456 mL	0.2913 mL	0.7282 mL
	60 mM	0.0243 mL	0.1214 mL	0.2427 mL	0.6068 mL
	80 mM	0.0182 mL	0.0910 mL	0.1821 mL	0.4551 mL
	100 mM	0.0146 mL	0.0728 mL	0.1456 mL	0.3641 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Rehmannioside D

2 Cited Publications

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Rehmannioside D

Apoptosis Compound Library

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Publications Citing Use of MedChemExpress (MCE) Rehmannioside D

All Sirtuin Isoforms

All Bcl-2 Family Isoforms

All Caspase Isoforms

Biological Activity

Chemical Information

Publications (2)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Rehmannioside D Related Classifications

HY-N0912 Related Classifications