製品番号: HY-155736

MAPK-IN-2

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MAPK-IN-2 (compound 3h) is a potent MAPK inhibitor with antineoplastic activity. MAPK-IN-2 inhibits cancer cell proliferation among serval cancer cell lines, and suppresses MAPK pathway with potant efficacy (EGFR^WT IC₅₀=281 nM, c-MET IC₅₀=205 nM, B-RAF^WT IC₅₀=112 nM, and CDK4/6 IC₅₀=95 and 184 nM, respectively). MAPK-IN-2 even shows a remarkable potency against mutated EGFR and B-RAF (EGFR^T790M IC₅₀=69 nM and B-RAF^V600E IC₅₀=83 nM).

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。

純度: 99.66%
CAS 番号: 3052814-46-4
分子式: C₂₀H₁₁Cl₂N₃O
分子量:380.23

保管条件:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

MAPK-IN-2 構造式

CAS 番号: 3052814-46-4

容量

価格（税別）

在庫状況

数量

Amount: USD 0.00

* アイテムを追加する前、数量をご選択ください

Top Publications Citing

Use of MCE Products

Antibody-Drug Conjugates

One-stop solution for XDC (ADC/PDC/RDC/ApDC/FDC/ISAC/VDC/SMDC)

生物活性
- 製品説明
- IC₅₀ & Target
- Cellular Effect
化学情報
溶剤 & 溶解度
- 体外
- 体内
純度とドキュメンテーション
参考文献
Help & FAQs
関連分類
Bioz

生物活性

製品説明

IC₅₀ & Target

IC50: 281 nM (EGFR^WT), 69 nM (EGFR^T790M), 205 nM (c-MET), 112 nM (B-RAF^WT), 83 nM (B-RAF^V600E), 95 nM (CDK4), 184 nM (CDK6)^[1]

p38 MAPK アイソフォーム固有の製品をすべて表示 :

CDK アイソフォーム固有の製品をすべて表示 :

Raf アイソフォーム固有の製品をすべて表示 :

EGFR アイソフォーム固有の製品をすべて表示 :

Cellular Effect

Cell Line	Type	Value	Description	References
FHC	GI₅₀	26.84 μM Compound: 3h	Cytotoxicity against human FHC cells assessed as cell growth inhibition incubated for 4 days by MTT assay Cytotoxicity against human FHC cells assessed as cell growth inhibition incubated for 4 days by MTT assay	[PMID: 37506545]
HCT-116	GI₅₀	2.53 μM Compound: 3h	Cytotoxicity against human HCT-116 cells assessed as cell growth inhibition incubated for 4 days by MTT assay Cytotoxicity against human HCT-116 cells assessed as cell growth inhibition incubated for 4 days by MTT assay	[PMID: 37506545]
HEK293	GI₅₀	22.4 μM Compound: 3h	Cytotoxicity against human HEK293 cells assessed as cell growth inhibition incubated for 4 days by MTT assay Cytotoxicity against human HEK293 cells assessed as cell growth inhibition incubated for 4 days by MTT assay	[PMID: 37506545]
HOP-92	GI₅₀	0.75 μM Compound: 3h	Cytotoxicity against human HOP-92 cells assessed as cell growth inhibition incubated for 4 days by MTT assay Cytotoxicity against human HOP-92 cells assessed as cell growth inhibition incubated for 4 days by MTT assay	[PMID: 37506545]
MRC5	GI₅₀	28.48 μM Compound: 3h	Cytotoxicity against human MRC5 cells assessed as cell growth inhibition incubated for 4 days by MTT assay Cytotoxicity against human MRC5 cells assessed as cell growth inhibition incubated for 4 days by MTT assay	[PMID: 37506545]
RXF 393	GI₅₀	3.12 μM Compound: 3h	Cytotoxicity against human RXF 393 cells assessed as cell growth inhibition incubated for 4 days by MTT assay Cytotoxicity against human RXF 393 cells assessed as cell growth inhibition incubated for 4 days by MTT assay	[PMID: 37506545]
SR	GI₅₀	2.77 μM Compound: 3h	Cytotoxicity against human SR cells assessed as cell growth inhibition incubated for 4 days by MTT assay Cytotoxicity against human SR cells assessed as cell growth inhibition incubated for 4 days by MTT assay	[PMID: 37506545]

化学情報

CAS 番号 3052814-46-4
性状 Solid
分子量 380.23
分子式 C₂₀H₁₁Cl₂N₃O
Color Light yellow to orange
SMILES

O=C1C(C#N)=C(C2=CC=C(Cl)C=C2Cl)C=C(C3=CNC4=C3C=CC=C4)N1
輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶剤 & 溶解度

体外:

DMSO : 16.67 mg/mL (43.84 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.6300 mL

13.1499 mL

26.2999 mL

5 mM

0.5260 mL

2.6300 mL

5.2600 mL

10 mM

0.2630 mL

1.3150 mL

2.6300 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

モル濃度計算機

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

濃度濃度

体積体積

分子量分子量

希釈計算機

濃度 _(開始) × 体積 _(開始) = 濃度 _(終了) × 体積 _(終了)

濃度 _(開始) 濃度 _(開始)

体積 _(開始) 体積 _(開始)

濃度 _(終了) 濃度 _(終了)

体積 _(終了) 体積 _(終了)

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: 1.67 mg/mL (4.39 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 1.67 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

純度とドキュメンテーション

バッチを選択:

純度: 99.98%

Assay: 99.98%

ee.: 99.98%

データシート (282 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
元素分析報告書 (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
取扱説明書 (2659 KB)

参考文献

[1]. Saleh MM, et al. The mystery of titan hunter: Rationalized striking of the MAPK pathway via Newly synthesized 6-Indolylpyridone-3-Carbonitrile derivatives. Eur J Med Chem. 2023 Nov 5;259:115675. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.6300 mL	13.1499 mL	26.2999 mL	65.7497 mL
	5 mM	0.5260 mL	2.6300 mL	5.2600 mL	13.1499 mL
	10 mM	0.2630 mL	1.3150 mL	2.6300 mL	6.5750 mL
	15 mM	0.1753 mL	0.8767 mL	1.7533 mL	4.3833 mL
	20 mM	0.1315 mL	0.6575 mL	1.3150 mL	3.2875 mL
	25 mM	0.1052 mL	0.5260 mL	1.0520 mL	2.6300 mL
	30 mM	0.0877 mL	0.4383 mL	0.8767 mL	2.1917 mL
	40 mM	0.0657 mL	0.3287 mL	0.6575 mL	1.6437 mL

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MAPK-IN-2

Cell Cycle/DNA Damage Compound Library

Immunology/Inflammation Compound Library

JAK/STAT Compound Library

Kinase Inhibitor Library

MAPK Compound Library

Protein Tyrosine Kinase Compound Library

Anti-Cancer Compound Library

Anti-Aging Compound Library

Differentiation Inducing Compound Library

Reprogramming Compound Library

Oxygen Sensing Compound Library

Ferroptosis Compound Library

Pyroptosis Compound Library

Glutamine Metabolism Compound Library

Anti-Hepatitis C Virus Compound Library

Anti-Breast Cancer Compound Library

Anti-Lung Cancer Compound Library

Anti-Pancreatic Cancer Compound Library

Anti-Blood Cancer Compound Library

Anti-Cancer Metabolism Compound Library

Neurodegenerative Disease-related Compound Library

Anti-Obesity Compound Library

Angiogenesis-Related Compound Library

Anti-Liver Cancer Compound Library

Anti-Colorectal Cancer Compound Library

Antidepressant Compound Library

Anti-Prostate Cancer Compound Library

Anti-Pulmonary Fibrosis Compound Library

Cancer Stem Cells Compound Library

Pain-Related Compound Library

Membrane Protein-targeted Compound Library

Membrane Receptor-targeted Compound Library

Cytokine Inhibitors Library

Serine/Threonine Kinase Inhibitor Library

Anti-Hematopathy Compound Library

Anti-Ovarian Cancer Compound Library

Multi-Target Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Anti-Gastric Cancer Compound Library

Anti-Brain Cancer Compound Library

Protein Kinase Compound Library

Cell Proliferation Compound Library

High-Throughput Bioactive Compound Library

Anti-Rheumatic Arthritis Compound Library

Posttranslational Modification Library

High-Efficiency Gene Editing Compound Library

Lactylation Compound Library

p38 MAPK アイソフォーム固有の製品をすべて表示

CDK アイソフォーム固有の製品をすべて表示

Raf アイソフォーム固有の製品をすべて表示

EGFR アイソフォーム固有の製品をすべて表示

生物活性

化学情報

溶剤 & 溶解度

純度とドキュメンテーション

参考文献

Help & FAQs

Complete Stock Solution Preparation Table

MAPK-IN-2 関連分類

HY-155736 関連分類