Tefluthrin
Based on 1 Customer Validation
Tefluthrin is a pyrethroid insecticide with developmental toxicity. Tefluthrin modifies the Nav1.6 voltage-gated sodium channel, prolonging Nav1.6 channel inactivation and deactivation in HEK293 cells. Tefluthrin reduces L-type Ca2+ currents, inhibits K+ outward currents, and enhances spontaneous/oscillatory action currents. Tefluthrin induces oxidative stress in rat models, causing damage to blood, immune, and nucleic acid metabolism, chromosomal aberrations, and sperm malformations; it induces oxidative stress and p53‑caspase‑3 pathway apoptosis in zebrafish models, leading to cardiac-skeletal developmental malformations such as pericardial edema, spinal curvature, and decreased skeletal mineralization. Tefluthrin can be used for pest control and research on diseases such as neurodegenerative diseases.
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- Reinheit : 98.12%
- CAS. Nr.: 79538-32-2
- Formel: C17H14ClF7O2
- Molecular Weight:418.73
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Speicherung:Pure form -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Protokoll
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
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Biologische Aktivität
Beschreibung
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Nav1.6 |
Caspase 3 |
Bcl-2 |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| GH3 | EC50 |
3.2 μM
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Concentration-dependent increase of peak Na+ current amplitude in rat pituitary tumor GH3 cells assessed by whole-cell patch-clamp recordings.
Concentration-dependent increase of peak Na+ current amplitude in rat pituitary tumor GH3 cells assessed by whole-cell patch-clamp recordings.
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19378468 |
In Vitro
Tefluthrin (10 μM) mildly inhibits K+ outward currents in GH3 cells without altering current kinetic properties, increases the amplitude and firing of action currents, and induces oscillatory inward currents; it also increases the amplitude and frequency of spontaneous action currents in GT1-7 neurons[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
Tefluthrin (1-100 μg/L; 8 days) produces concentration-dependent developmental toxicity in zebrafish embryos-larvae, manifested as decreased survival rate, heart rate, and body length, pericardial edema, and spinal curvature, disrupts the transcription of cardiac-related genes and skeletal-related genes, and induces oxidative stress; it can activate p53-caspase-3-mediated apoptosis; Astaxanthin (HY-B2163) can alleviate Tefluthrin-induced oxidative stress and rescue the cardiac-skeletal developmental defects in Tefluthrin-exposed zebrafish larvae[3].
Tefluthrin (1.1 mg/kg; oral gavage; administered 7 times within 15 days) subacutely decreases sperm count, mitotic index, body weight/testis weight, liver and testis nucleic acid and protein contents, nuclease activity, RBC/Hb and immunoglobulin levels, and increases sperm morphological abnormalities and chromosomal aberrations in adult male albino rats; co-administration of a mixture of vitamins A, C and E alleviates the disturbances[5].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Wistar (male, 60 days old, 200-210 g)[2]
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Dosage:2.2, 4.4, 5.5 mg/kg bw/day
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Administration:p.o. (oral gavage); once daily; 6 consecutive days
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Result:Decreased DA and 5-HT tissue contents and their metabolites in rat striatum, hippocampus, prefrontal cortex, and hypothalamus, with the largest reductions in prefrontal cortex, and altered prefrontal cortex dopaminergic and serotoninergic gene expression.
- Reproductive and Developmental Toxicity Study
- Detection of Apoptosis
- TUNEL staining for apoptotic DNA fragmentation
- Annexin V plus membrane-impermeant dye apoptosis staining
- ROS/oxidative-stress fluorescent staining
- Mesenchymal stromal/stem cell osteogenic differentiation
- Apoptosis Solutions
- Ca2+ Staining Technique
Chemical Information
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CAS. Nr. 79538-32-2
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Appearance Liquid to low melt solid
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Molecular Weight 418.73
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Formel C17H14ClF7O2
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Color Off-white to gray
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SMILES
O=C(OCC1=C(C(F)=C(C(F)=C1F)C)F)[C@@H]2C(C)([C@@H]2/C=C(C(F)(F)F)\Cl)C
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Pure form -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Lösungsmittel & Löslichkeit
In Vitro:
DMSO : 100 mg/mL (238.82 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.97 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (5.97 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Reinheit & Dokumentation
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Data Sheet (288 KB)
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SDS (565 KB)
- English - EN (565 KB)
- Français - FR (565 KB)
- Deutsch - DE (565 KB)
- Norwegian - NO (565 KB)
- Español - ES (565 KB)
- Swedish - SV (565 KB)
- Italian - IT (565 KB)
- Korean - KR (565 KB)
- Portuguese - PT (565 KB)
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Handling Instructions (2659 KB)
Verweise
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.3882 mL | 11.9409 mL | 23.8817 mL | 59.7043 mL |
| 5 mM | 0.4776 mL | 2.3882 mL | 4.7763 mL | 11.9409 mL | |
| 10 mM | 0.2388 mL | 1.1941 mL | 2.3882 mL | 5.9704 mL | |
| 15 mM | 0.1592 mL | 0.7961 mL | 1.5921 mL | 3.9803 mL | |
| 20 mM | 0.1194 mL | 0.5970 mL | 1.1941 mL | 2.9852 mL | |
| 25 mM | 0.0955 mL | 0.4776 mL | 0.9553 mL | 2.3882 mL | |
| 30 mM | 0.0796 mL | 0.3980 mL | 0.7961 mL | 1.9901 mL | |
| 40 mM | 0.0597 mL | 0.2985 mL | 0.5970 mL | 1.4926 mL | |
| 50 mM | 0.0478 mL | 0.2388 mL | 0.4776 mL | 1.1941 mL | |
| 60 mM | 0.0398 mL | 0.1990 mL | 0.3980 mL | 0.9951 mL | |
| 80 mM | 0.0299 mL | 0.1493 mL | 0.2985 mL | 0.7463 mL | |
| 100 mM | 0.0239 mL | 0.1194 mL | 0.2388 mL | 0.5970 mL |
Tefluthrin Related Classifications
HY-W040171 Related Classifications
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Keywords
- Tefluthrin
- 79538-32-2
- Insecticide
- Sodium Channel
- Caspase
- Bcl-2 Family
- Reactive Oxygen Species (ROS)
- Apoptosis
- BK channels
- K+ outward current
- L-type Ca2+ current
- dopamine
- early afterdepolarizations
- neurodegenerative disorders
- pyrethroid insecticide
- serotonin
- sodium-channel inactivation
- ventricular arrhythmias
- voltage-gated sodium channel modulator
- Inhibitor
- inhibitor
- inhibit