2. Others Neuronal Signaling GPCR/G Protein Apoptosis Metabolic Enzyme/Protease PI3K/Akt/mTOR JAK/STAT Signaling Stem Cell/Wnt NF-κB
  3. Isotope-Labeled Compounds mGluR Ferroptosis Environmental Pollutants Endogenous Metabolite HIF/HIF Prolyl-Hydroxylase mTOR STAT NF-κB
  4. L-Glutamine-13C5

L-Glutamine-13C5  (Synonyms: L-Glutamic acid 5-amide-13C5)

Cat. No.: HY-N0390S1 Purity: 99.93%
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L-Glutamine-13C5 is the 13C-labeled L-Glutamine (HY-N0390). L-Glutamine is an orally active nutritional agent and cellular metabolism regulator. L-Glutamine is taken up in a Na+-dependent manner and targets multiple key molecules including glutaminase, mTORC1, NF-κB, STAT-3 and HIF-1α. L-Glutamine enhances glutaminolytic catabolism, drives the conversion of glutamate to α-ketoglutarate, thereby regulating gene expression, integrating metabolic signals, mediating glutamine flux and maintaining redox homeostasis. L-Glutamine also promotes cell proliferation, osteogenic differentiation and fracture healing, exerts neuroprotective and cardioprotective effects, and inhibits osteoarthritis. L-Glutamine can be applied to research related to osteoporosis, osteoarthritis, ischemic stroke and acute cantharidin-induced cardiotoxicity.

For research use only. We do not sell to patients.

CAS No. : 184161-19-1

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water
ready for reconstitution
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Customer Review

Based on 9 publication(s) in Google Scholar

Other Forms of L-Glutamine-13C5:

L-Glutamine-13C5 Related Antibodies

Top Publications Citing Use of Products

    L-Glutamine-13C5 purchased from MedChemExpress. Usage Cited in: Nat Metab. 2025 Sep;7(9):1871-1888.  [Abstract]

    Isotopologue abundance of citrate/isocitrate, α-ketoglutarate, succinate, fumarate, malate, and aspartate in ctrl, A42 KO, A16 KO, and A16/A42 DKO HeLa cells with L-Glutamine-13C5 tracing (2 mM for 2 hrs) with naturally occurring 13C abundance correction.

    L-Glutamine-13C5 purchased from MedChemExpress. Usage Cited in: Nat Metab. 2025 Sep;7(9):1871-1888.  [Abstract]

    Stable isotope tracing of A16/A42 DKO K562 cells using L-Glutamine-13C5 (2 mM for 2 h).

    L-Glutamine-13C5 purchased from MedChemExpress. Usage Cited in: Nat Struct Mol Biol. 2024 May 20.  [Abstract]

    Isotopomer distributions of citrate from L-Glutamine-13C5 (2 mM for 24 h) was analyzed in shCtrl and shHES4 A549 cells reconstituted with or without WT rHES4.

    L-Glutamine-13C5 purchased from MedChemExpress. Usage Cited in: Nat Struct Mol Biol. 2024 May 20.  [Abstract]

    Schematic of isotopomer distribution of indicated metabolites derived from L-Glutamine-13C5 (2 mM for 24 h) . 13C atoms are depicted in blue.

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    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    L-Glutamine-13C5 is the 13C-labeled L-Glutamine (HY-N0390). L-Glutamine is an orally active nutritional agent and cellular metabolism regulator. L-Glutamine is taken up in a Na+-dependent manner and targets multiple key molecules including glutaminase, mTORC1, NF-κB, STAT-3 and HIF-1α. L-Glutamine enhances glutaminolytic catabolism, drives the conversion of glutamate to α-ketoglutarate, thereby regulating gene expression, integrating metabolic signals, mediating glutamine flux and maintaining redox homeostasis. L-Glutamine also promotes cell proliferation, osteogenic differentiation and fracture healing, exerts neuroprotective and cardioprotective effects, and inhibits osteoarthritis. L-Glutamine can be applied to research related to osteoporosis, osteoarthritis, ischemic stroke and acute cantharidin-induced cardiotoxicity[1][2][3][4].

    Application

    1. This compound can be used as a tracer.
    2. This compound can be used as an internal standard for quantitative analysis by NMR, GC-MS, or LC-MS.

    In Vitro

    Stable heavy isotopes of hydrogen, carbon, and other elements have been incorporated into drug molecules, largely as tracers for quantitation during the drug development process. Deuteration has gained attention because of its potential to affect the pharmacokinetic and metabolic profiles of drugs[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    L-Glutamine-13C5 Related Antibodies
    Molecular Weight

    151.11

    Formula

    13C5H10N2O3
    CAS No.
    Unlabeled CAS
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    N[13C]([13CH2][13CH2][13C@H](N)[13C](O)=O)=O
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    H2O : 10 mg/mL (66.18 mM; ultrasonic and warming and heat to 60°C)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 6.6177 mL 33.0885 mL 66.1770 mL
    5 mM 1.3235 mL 6.6177 mL 13.2354 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

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    In Vivo:

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 16.67 mg/mL (110.32 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.93%

    SDS (252 KB)

    COA (248 KB) HNMR (280 KB) RP-HPLC (199 KB) MS (80 KB) OR (250 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 6.6177 mL 33.0885 mL 66.1770 mL 165.4424 mL
    5 mM 1.3235 mL 6.6177 mL 13.2354 mL 33.0885 mL
    10 mM 0.6618 mL 3.3088 mL 6.6177 mL 16.5442 mL
    15 mM 0.4412 mL 2.2059 mL 4.4118 mL 11.0295 mL
    20 mM 0.3309 mL 1.6544 mL 3.3088 mL 8.2721 mL
    25 mM 0.2647 mL 1.3235 mL 2.6471 mL 6.6177 mL
    30 mM 0.2206 mL 1.1029 mL 2.2059 mL 5.5147 mL
    40 mM 0.1654 mL 0.8272 mL 1.6544 mL 4.1361 mL
    50 mM 0.1324 mL 0.6618 mL 1.3235 mL 3.3088 mL
    60 mM 0.1103 mL 0.5515 mL 1.1029 mL 2.7574 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    L-Glutamine-13C5 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    L-Glutamine-13C5184161-19-1L-Glutamic acid 5-amide-13C5Isotope-Labeled CompoundsmGluRFerroptosisEnvironmental PollutantsEndogenous MetaboliteHIF/HIF Prolyl-HydroxylasemTORSTATNF-κBMetabotropic glutamate receptorsEnvironmental ContaminantsHypoxia-inducible factorsHIFsHIF-PHMammalian target of RapamycinNuclear factor-κBNuclear factor-kappaBInhibitorinhibitorinhibit

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