1. Immunology/Inflammation Apoptosis Metabolic Enzyme/Protease Cell Cycle/DNA Damage
  2. Toll-like Receptor (TLR) PKD HSP Bcl-2 Family Interleukin Related
  3. Polyinosinic-polycytidylic acid

Polyinosinic-polycytidylic acid  (Synonyms: Poly(I:C))

Cat. No.: HY-107202 Assay: 99.40%
COA Handling Instructions

Polyinosinic-polycytidylic acid (Poly (I:C)), a synthetic analog of double-stranded RNA, is an agonist of toll-like receptor (TLR)-3. Polyinosinic-polycytidylic acid facilitates tumor regression and has a disruptive effect on the airway epithelial barrier. Polyinosinic-polycytidylic acid has protective effects against cerebral ischemia/reperfusion (I/R) injury and can be used as vaccine adjuvant to enhance innate and adaptive immune responses .

For research use only. We do not sell to patients.

Polyinosinic-polycytidylic acid Chemical Structure

Polyinosinic-polycytidylic acid Chemical Structure

CAS No. : 24939-03-5

Size Price Stock Quantity
5 mg USD 58 In-stock
10 mg USD 90 In-stock
25 mg USD 210 In-stock
50 mg USD 360 In-stock
100 mg USD 570 In-stock
200 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 13 publication(s) in Google Scholar

Top Publications Citing Use of Products

View All Toll-like Receptor (TLR) Isoform Specific Products:

View All PKD Isoform Specific Products:

View All HSP Isoform Specific Products:

View All Bcl-2 Family Isoform Specific Products:

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Polyinosinic-polycytidylic acid (Poly (I:C)), a synthetic analog of double-stranded RNA, is an agonist of toll-like receptor (TLR)-3. Polyinosinic-polycytidylic acid facilitates tumor regression and has a disruptive effect on the airway epithelial barrier. Polyinosinic-polycytidylic acid has protective effects against cerebral ischemia/reperfusion (I/R) injury and can be used as vaccine adjuvant to enhance innate and adaptive immune responses [1][2][3][4][5].

IC50 & Target

Bcl-2

 

Bax

 

IL-17A

 

IL-13

 

HSP70

 

In Vitro

Polyinosinic-polycytidylic acid (0.5-5 μg/mL, 3-24 h) induces a dose- and time-dependent increase in paracellular permeability of immortalized airway epithelial cells[4].
Polyinosinic-polycytidylic acid (5 μg/mL, 24 h) does not have cytotoxicity to 16HBE14o- cells[4].
Polyinosinic-polycytidylic acid (5 μg/mL, 6 h) induces disruption of epithelial apical junctional complexes (AJCs) and tight junctions (TJs) in 16HBE14o- cells[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay[4]

Cell Line: 16HBE14o- cells
Concentration: 5 μg/mL
Incubation Time: 24 h
Result: Did not lead to significant accumulation of LDH in cell-culture medium
In Vivo

Polyinosinic-polycytidylic acid (2.5-10 mg/mL, Stereotaxic injection, single dose) induces a sustained inflammatory reaction in the substantia nigra (SN) and in the dorsolateral striatum. [2].
Polyinosinic-polycytidylic acid (10 μg/mouse, Intraperitoneal injection, single dose) decreases lung tumor growth in mice[3].
Polyinosinic-polycytidylic acid (1.25 mg/kg, Intraperitoneal injection, single dose) exerts therapeutic effects against cerebral I/R injury through the downregulation of TLR4/MyD88 signaling via TLR3 in MCAO model mice[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: lung tumor-bearing mice[3]
Dosage: 10 μg/mouse
Administration: Intraperitoneal injection (i.p.)
Result: Induced a significant decrease in the growth of pulmonary metastases in tumor-bearing mice.
Reduced the amount of lung foci to ≈ 40%.
Significantly increased BAL fluid cell numbers.
Increased the level of INF-γ and IL-17A, decreased the levels of IL-13.
Increased TLR3 expression.
Animal Model: Middle cerebral artery occlusion (MCAO) model mice[5]
Dosage: 1.25 mg/kg
Administration: Intraperitoneal injection (i.p.)
Result: Reduced focal cerebral I/R injury.
Increased the expression of Bcl2, Hsp27, and Hsp70, decreased Bax expression, and reduced cellular degeneration and apoptosis.
Protected against cerebral ischemia and conferred protection against cerebral I/R injury through the downregulation of TLR4 signaling via TLR3.
Formula

(C10H13N4O8P)x.(C9H14N3O8P)x

CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O[C@H]1[C@@H](O[C@H](COP(O)(O)=O)[C@H]1O)N2C3=C(C(NC=N3)=O)N=C2.O[C@H]4[C@H](N5C(N=C(N)C=C5)=O)O[C@H](COP(O)(O)=O)[C@H]4O.[x].[x]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

H2O : 50 mg/mL (Need ultrasonic)

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 100 mg/mL (Infinity mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.40%

References
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

 

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

     

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Polyinosinic-polycytidylic acid
Cat. No.:
HY-107202
Quantity:
MCE Japan Authorized Agent: