TPP-C8-CO-DSG bromide
TPP-C8-CO-DSG bromide is a derivative of Diosgenin (HY-N0177) and an anticancer agent. TPP-C8-CO-DSG bromide downregulates Bcl-2 expression and upregulates Bax expression. TPP-C8-CO-DSG bromide promotes the cleavage of Caspase 9, Caspase 3 and PARP-1. TPP-C8-CO-DSG bromide depolarizes mitochondrial membrane potential, reduces intracellular ATP production, and induces intracellular ROS accumulation. TPP-C8-CO-DSG bromide promotes Apoptosis. TPP-C8-CO-DSG bromide exhibits anticancer activity against prostate cancer, breast cancer, lung adenocarcinoma, cervical cancer and colorectal cancer. TPP-C8-CO-DSG bromide can be used in the research of cervical cancer.
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- Fòrmula: C54H72BrO4P
- Peso molecular:896.02
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Almacenamiento:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Actividad biológica
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Chemical Information
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- Pureza y Documentación
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Help & FAQs
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Actividad biológica
Descripciòn
IC50 & Target
[1]|
Bcl-2 |
Bax |
Caspase 9 |
Caspase 3 |
PARP-1 |
In Vitro
TPP-C8-CO-DSG (bromide) (0.02-0.5 μM; 36 h) dose-dependently reduces intracellular ATP production in HeLa cells, causing an 80.1% reduction at the highest tested concentration of 0.5 μM after 36 h of treatment[1].
TPP-C8-CO-DSG (bromide) (0.02-0.5 μM; 36 h) dose-dependently induces early and late apoptosis in HeLa cells, with a combined apoptotic rate of 48.04% observed at 0.5 μM after 36 h of treatment[1].
TPP-C8-CO-DSG (bromide) (0.02-0.5 μM; 36 h) induces apoptosis in HeLa cells via a mitochondria-associated pathway, characterized by upregulation of pro-apoptotic proteins, downregulation of anti-apoptotic Bcl-2, and activation of the caspase cascade after 36 h of treatment[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HeLa
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Concentration:0.02-0.5 μM
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Incubation Time:36 h
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Result:Showed faint red and blue fluorescence in cells treated with 0.02 μM, while cells treated with 0.1 μM and 0.5 μM showed increased intensity of bright red and blue fluorescence, indicating a dose-dependent increase in apoptosis and necrosis.\nCaused a concentration-dependent increase in early and late apoptotic cell populations.
Increased early apoptotic cells from 6.25% (control) to 30.02% (0.5 μM), while late apoptotic cells increased from 2.31% (control) to 18.02% (0.5 μM).
Made the early and late apoptosis rates 18.0- and 45.1-fold higher than the control, respectively.
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Cell Line:HeLa
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Concentration:0.02-0.5 μM
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Incubation Time:36 h
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Result:Upregulated expression of pro-apoptotic proteins Bax, cytochrome c, cleaved caspase 9, cleaved caspase 3, and cleaved PARP-1.
Downregulated expression of anti-apoptotic protein Bcl-2.
Increased the ratios of cleaved caspase 9 to caspase 9, cleaved caspase 3 to caspase 3, and cleaved PARP-1 to PARP-1.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:AB strain (larval stage, 48 h post-fertilization)[1]
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Dosage:1.6 μg/mL
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Administration:48-hour treatment
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Result:Significantly reduced the fluorescence intensity of HeLa tumor xenografts in zebrafish, indicating inhibition of HeLa cell proliferation.
- RNA extraction experimental
- Mitochondrial membrane-potential fluorescent assay
- Detection of Apoptosis
- TUNEL staining for apoptotic DNA fragmentation
- Annexin V plus membrane-impermeant dye apoptosis staining
- ROS/oxidative-stress fluorescent staining
- Fluorescent plasma-membrane potential dye assay
- Mitochondrial membrane-potential and mitochondrial mass staining
- Apoptosis Solutions
- Breast Cancer Modeling
Chemical Information
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Peso molecular 896.02
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Fòrmula C54H72BrO4P
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SMILES
O=C(CCCCCCCC[P+](C1=CC=CC=C1)(C2=CC=CC=C2)C3=CC=CC=C3)O[C@@]4(CC5=CC[C@@]6([C@@]([H])([C@]5(CC4)C)CC[C@]7([C@]6(C[C@]8([C@@]7([C@@]([H])([C@]9(O8)CC[C@@](C)(CO9)[H])C)[H])[H])[H])C)[H])[H].[Br-]
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureza y Documentación
Referencias
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
TPP-C8-CO-DSG bromide Related Classifications
HY-184737 Related Classifications
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Keywords
- TPP-C8-CO-DSG
- Drug Derivative
- Bcl-2 Family
- Caspase
- PARP
- Mitochondrial Metabolism
- ATP Synthase
- Reactive Oxygen Species (ROS)
- Apoptosis
- Drug derivative
- poly ADP ribose polymerase
- cytochrome c
- Bax
- caspase 9
- apoptosis
- normal human liver cells
- Bcl-2
- cancer cell lines
- caspase 3
- PARP-1
- zebrafish xenograft model
- Inhibitor
- inhibitor
- inhibit