2. Anti-infection Autophagy Apoptosis Cell Cycle/DNA Damage Epigenetics PI3K/Akt/mTOR
  3. Bacterial Autophagy MDM-2/p53 Caspase PARP DNA-PK Akt
  4. Sulfabenzamide

Sulfabenzamide  (Synonyms: N-Sulfanilylbenzamide)

Cat. No.: HY-B0960 Purity: 99.68%
COA
SDS
Handling Instructions Technical Support

Sulfabenzamide (N-Sulfanilylbenzamide) is a sulfonamide antibacterial agent. Sulfabenzamide exhibit antibacterial activity against Staphylococcus aureus (ATCC 25923) and Escherichia coli (ATCC 8739). Sulfabenzamide can promote autophagic cell autophagy in breast cancer cells through p53/ DRAM pathway. Sulfabenzamide increases caspase-3 activity, deactivates PARP1 and DNA-PK, downregulates AKT1 and AKT2. Sulfabenzamide can be used for the researches of breast cancer and bacterial infections.

For research use only. We do not sell to patients.

Sulfabenzamide

Sulfabenzamide Chemical Structure

CAS No. : 127-71-9

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
500 mg In-stock
1 g   Get quote  
5 g   Get quote  

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Sulfabenzamide:

Sulfabenzamide Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Sulfabenzamide

View All Caspase Isoform Specific Products:

View All Isoforms
Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

View All PARP Isoform Specific Products:

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PARP PARP1 PARP2 PARP3 PARP4 TNKS1/PARP5A TNKS2/PARP5B PARP7 PARP10 PARP14 PARP15 PARP12 PARP16

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View All Isoforms
Akt Akt1 Akt2 Akt3

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Sulfabenzamide (N-Sulfanilylbenzamide) is a sulfonamide antibacterial agent. Sulfabenzamide exhibit antibacterial activity against Staphylococcus aureus (ATCC 25923) and Escherichia coli (ATCC 8739). Sulfabenzamide can promote autophagic cell autophagy in breast cancer cells through p53/ DRAM pathway. Sulfabenzamide increases caspase-3 activity, deactivates PARP1 and DNA-PK, downregulates AKT1 and AKT2. Sulfabenzamide can be used for the researches of breast cancer and bacterial infections[1][2].

IC50 & Target[1]

Caspase 3

 

Akt1

 

Akt2

 

PARP1

 

In Vitro

Sulfabenzamide promotes autophagic cell death in T-47D breast cancer cells primarily via induction of autophagy through the p53/DRAM pathway, with attenuation of the AKT/mTOR survival pathway and downregulation of AKT1 and AKT2[1].
Sulfabenzamide (18 h) and its metal complexes exhibit antibacterial activity against Staphylococcus aureus (ATCC 25923) and Escherichia coli (ATCC 8739); the MIC values of free sulfabenzamide and zinc(II)-sulfabenzamide complex against the two strains are both 1000 ppm[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Sulfabenzamide Related Antibodies
Molecular Weight

276.32

Formula

C13H12N2O3S
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(NS(=O)(C1=CC=C(N)C=C1)=O)C2=CC=CC=C2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (361.90 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.6190 mL 18.0950 mL 36.1899 mL
5 mM 0.7238 mL 3.6190 mL 7.2380 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (9.05 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (9.05 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.68%

SDS (396 KB)

COA (248 KB) LCMS (93 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.6190 mL 18.0950 mL 36.1899 mL 90.4748 mL
5 mM 0.7238 mL 3.6190 mL 7.2380 mL 18.0950 mL
10 mM 0.3619 mL 1.8095 mL 3.6190 mL 9.0475 mL
15 mM 0.2413 mL 1.2063 mL 2.4127 mL 6.0317 mL
20 mM 0.1809 mL 0.9047 mL 1.8095 mL 4.5237 mL
25 mM 0.1448 mL 0.7238 mL 1.4476 mL 3.6190 mL
30 mM 0.1206 mL 0.6032 mL 1.2063 mL 3.0158 mL
40 mM 0.0905 mL 0.4524 mL 0.9047 mL 2.2619 mL
50 mM 0.0724 mL 0.3619 mL 0.7238 mL 1.8095 mL
60 mM 0.0603 mL 0.3016 mL 0.6032 mL 1.5079 mL
80 mM 0.0452 mL 0.2262 mL 0.4524 mL 1.1309 mL
100 mM 0.0362 mL 0.1809 mL 0.3619 mL 0.9047 mL
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Sulfabenzamide Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Sulfabenzamide127-71-9N-SulfanilylbenzamideBacterialAutophagyMDM-2/p53CaspasePARPDNA-PKAktpoly ADP ribose polymerase DNA-dependent protein kinasePKBProtein kinase BStaphylococcus aureus (ATCC 25923)T-47D breast cancer cellsPTENcaspase-3ATG5AKT/mTOR pathwayPARP1DRAMp53Inhibitorinhibitorinhibit

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