Cat. No.: HY-113952

Actinonin

(Synonyms: (-)-Actinonin)

7 Cited Publications

Customer Review

Based on 7 publication(s) in Google Scholar

Actinonin ((-)-Actinonin) is a naturally occurring antibacterial agent produced by Actinomyces. Actinonin inhibits aminopeptidase M, aminopeptidase N and leucine aminopeptidase. Actinonin is a potent reversible peptide deformylase (PDF) inhibitor with a K_i of 0.28 nM. Actinonin also inhibits MMP-1, MMP-3, MMP-8, MMP-9, and hmeprin α with K_i values of 300 nM, 1,700 nM, 190 nM, 330 nM, and 20 nM, respectively. Actinonin is an apoptosis inducer. Actinonin has antiproliferative and antitumor activities.

For research use only. We do not sell to patients.

Purity: 98.37%
CAS No.: 13434-13-4
Formula: C₁₉H₃₅N₃O₅
Molecular Weight:385.50

Storage:

Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month

Actinonin Chemical Structure

CAS No.: 13434-13-4

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (7)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

Ki: 0.28 nM (Peptide deformylase (PDF))^[2], 300 nM (MMP-1), 1,700 nM (MMP-3), 190 nM (MMP-8), 330 nM (MMP-9)^[3], and 20 nM (hmeprin α)^[5]
Apoptosis^[1]
Aminopeptidase M, Aminopeptidase N and Leucine aminopeptidase^[1]

All Aminopeptidase Isoforms :

CD13
LTA4H

All MMP Isoforms :

All Antibiotic Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
BMDM	CC₅₀	>193 μg/mL Compound: Actinonin	Cytotoxicity against mouse BMDM cells Cytotoxicity against mouse BMDM cells	[PMID: 35307568]
BMDM	CC₅₀	>500 μM Compound: Actinonin	Cytotoxicity against mouse BMDM cells Cytotoxicity against mouse BMDM cells	[PMID: 35307568]
HCT-116	IC₅₀	22.6 μM Compound: Actinonin	Antiproliferative activity against human HCT116 cells assessed as reduction in cell growth measured after 48 hrs by CCK8 assay Antiproliferative activity against human HCT116 cells assessed as reduction in cell growth measured after 48 hrs by CCK8 assay	[PMID: 32551649]
HEK293	IC₅₀	15 μM Compound: 1	Cytotoxicity against human HEK293 cells Cytotoxicity against human HEK293 cells	[PMID: 21719286]
HL-60	IC₅₀	100 μM Compound: 1	Cytotoxicity against human vincristine-resistant HL60 cells Cytotoxicity against human vincristine-resistant HL60 cells	[PMID: 21719286]
HL-60	IC₅₀	16 μM Compound: 1	Cytotoxicity against human HL60 cells Cytotoxicity against human HL60 cells	[PMID: 21719286]
Jurkat	IC₅₀	40 μM Compound: 1	Cytotoxicity against human Jurkat cells Cytotoxicity against human Jurkat cells	[PMID: 21719286]
MOLT-3	IC₅₀	45 μM Compound: 1	Cytotoxicity against human MOLT3 cells Cytotoxicity against human MOLT3 cells	[PMID: 21719286]

In Vitro

Actinonin inhibits cell growth in various human tumor cell lines. The IC₅₀ of 4, 6.9, 12.8, 16.6, 27.4, 15.7 and 49.3 μM for Raji cells, MDA-MB-468 cells,PC3 cells, SK-LC-19 cells, Hela cells, HT-1080 cells and AL67 cells, respectively^[1].
HsPDF is a critical target of actinonin and that the inhibition of this protein in the mitochondria leads to cell death in tumor cells. Actinonin treatment of cells led to a tumor-specific mitochondrial membrane depolarization and ATP depletion in a time- and dose-dependent manner^[1].
Actinonin is a potent inhibitor of all three forms (Zn-, Ni-, and Fe-) of peptide deformylases from both S. aureus and E. coli bacteria. Under the assay conditions, the IC₅₀ values for Actinonin are 90, 3, 0.8, and 11 nM for Zn-PDF (E. coli), Ni-PDF (E. coli), Fe-PDF (E. coli), and Ni-PDF (S. aureus), respectively^[2].
Actinonin is active against Gram-positive bacteria, including S. aureus (MIC value of 8-16 μg/mL), Streptococcus pyogenes (MIC value of 8 μg/mL) and Streptococcus epidermidis (MIC value of 2-4 μg/mL). Actinonin is also active against fastidious Gramne-gative bacteria, such as H. influenzae (MIC value of 1-2 μg/mL), Moraxella catarrhalis (MIC value of 0.5 μg/mL), and Neisseria gonorrheae (MIC value of 1-4 μg/mL). Actinonin is very active against the H. influenzae acr (MIC value of 0.13 μg/mL) and E. coli acr (MIC value of 0.25 μg/mL) efflux pump mutants^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Actinonin has been safely administered to mice as an antibiotic at doses up to 400 mg/kg. Actinonin does not appear to have significant toxicity to normal tissues, despite its antitumor activity in vitro. Remarkably, Actinonin exhibits significant antitumor activity when given i.p. or orally in a CWR22 human prostate tumor xenograft model in nude mice. During treatment, the animals show no signs of toxicity^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 13434-13-4

Appearance Solid

Molecular Weight 385.50

Formula C₁₉H₃₅N₃O₅

Color White to off-white

SMILES

O=C(N[C@H](C(N1[C@H](CO)CCC1)=O)C(C)C)[C@H](CCCCC)CC(NO)=O

Synonyms

(-)-Actinonin

Structure Classification

Others

Initial Source

Microorganisms

Marine natural products

actinomycete

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder -20°C 3 years

In solvent -80°C 6 months

-20°C 1 month

Publications (7)

All Cell Proliferation/Viability AssayWB

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Journal Impact Factor

Journal Impact Factor

Most Recent

mBio

The major role of Listeria monocytogenes folic acid metabolism during infection is the generation of N-formylmethionine. [Abstract]

2023 Oct 31;14(5):e0107423. PMID: 37695058

Sci Rep

Cytosolic N-terminal formyl-methionine deformylation derives cancer stem cell features and tumor progression. [Abstract]

2024 Jun 28;14(1):14900. PMID: 38942903

Actinonin purchased from MedChemExpress. Usage Cited in: Sci Rep. 2024 Jun 28;14(1):14900. [Abstract]

The growth of vector alone and EcPDF3f SW480 cells in the absence or presence of Actinonin (5 µg/ml final concentration).

Actinonin purchased from MedChemExpress. Usage Cited in: Sci Rep. 2024 Jun 28;14(1):14900. [Abstract]

Immunoblotting of SOX2 in control and EcPDF3f SW480 cells with or without Actinonin (2 µg/ml) treatment for 2 days.

J Mol Biol

TRIM52 Ubiquitin Ligase Acts as a Key Recognition Component of the Mammalian fMet/N-degron Pathway. [Abstract]

2025 Sep 19:169453. PMID: 40976346

Exp Cell Res

Inhibition of human peptide deformylase by actinonin sensitizes glioblastoma cells to temozolomide chemotherapy. [Abstract]

2022 Nov 15;420(2):113358. PMID: 36116558

Res Sq

2025 Jul 13.

Research Square Preprint

2024 Jan 31.

bioRxiv

2023 Oct 23.

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (129.70 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.5940 mL

12.9702 mL

25.9403 mL

5 mM

0.5188 mL

2.5940 mL

5.1881 mL

10 mM

0.2594 mL

1.2970 mL

2.5940 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Molecular Weight Molecular Weight

Dilution Calculator

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:

To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO    40% PEG300    5% Tween-80    45% Saline
Solubility: ≥ 5 mg/mL (12.97 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.

Protocol 2

Add each solvent one by one: 10% DMSO    90% (20% SBE-β-CD in Saline)
Solubility: 5 mg/mL (12.97 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

Protocol 3

Add each solvent one by one: 10% DMSO    90% Corn Oil
Solubility: ≥ 5 mg/mL (12.97 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg

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g

Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL

Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

%
+

+
%
Tween-80 + +

%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact
MedChemExpress (MCE) .

Tel: 609-228-6898
E-mail: [email protected]
Technical Support: [email protected]

Method for preparing in vivo working solution for animal experiments:
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Saline .

Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (278 KB)

SDS (393 KB)

English - EN (393 KB)

Français - FR (393 KB)

Deutsch - DE (393 KB)

Norwegian - NO (393 KB)

Español - ES (393 KB)

Swedish - SV (393 KB)

Italian - IT (393 KB)

Korean - KR (393 KB)

Portuguese - PT (393 KB)

COA (2659 KB)

Elemental Analysis Report (2659 KB)

HNMR (2659 KB)

CNMR (2659 KB)

RP-HPLC (2659 KB)

NP-HPLC (2659 KB)

MS (2659 KB)

LCMS (2659 KB)

IR (2659 KB)

GC (2659 KB)

OR (2659 KB)

SDS-PAGE (2659 KB)

SEC-HPLC (2659 KB)

Other Report (2659 KB)

Handling Instructions (2659 KB)

References

[1]. Lee MD, et al. Human mitochondrial peptide deformylase, a new anticancer target of actinonin-based antibiotics. J Clin Invest. 2004 Oct;114(8):1107-16. [Content Brief]

[2]. Chen DZ, et al. Actinonin, a naturally occurring antibacterial agent, is a potent deformylase inhibitor. Biochemistry. 2000 Feb 15;39(6):1256-62. [Content Brief]

[3]. Wahl, R.C., et al. Hydroxamate inhibitors of human gelatinase B (92 kDa). Bioorganic & Medicinal Chemistry Letters 5(4), 349-352 (1995).

[4]. Duke SO, et al. Modes of action of microbially-produced phytotoxins. Toxins (Basel). 2011 Aug;3(8):1038-64. [Content Brief]

[5]. Kruse MN, et al. Human meprin alpha and beta homo-oligomers: cleavage of basement membrane proteins and sensitivity to metalloprotease inhibitors. Biochem J. 2004 Mar 1;378(Pt 2):383-9. [Content Brief]

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg

DMSO 1 mM 2.5940 mL 12.9702 mL 25.9403 mL 64.8508 mL

5 mM 0.5188 mL 2.5940 mL 5.1881 mL 12.9702 mL

10 mM 0.2594 mL 1.2970 mL 2.5940 mL 6.4851 mL

15 mM 0.1729 mL 0.8647 mL 1.7294 mL 4.3234 mL

20 mM 0.1297 mL 0.6485 mL 1.2970 mL 3.2425 mL

25 mM 0.1038 mL 0.5188 mL 1.0376 mL 2.5940 mL

30 mM 0.0865 mL 0.4323 mL 0.8647 mL 2.1617 mL

40 mM 0.0649 mL 0.3243 mL 0.6485 mL 1.6213 mL

50 mM 0.0519 mL 0.2594 mL 0.5188 mL 1.2970 mL

60 mM 0.0432 mL 0.2162 mL 0.4323 mL 1.0808 mL

80 mM 0.0324 mL 0.1621 mL 0.3243 mL 0.8106 mL

100 mM 0.0259 mL 0.1297 mL 0.2594 mL 0.6485 mL

Actinonin Related Classifications

HY-113952 Related Classifications

Anti-infection Metabolic Enzyme/Protease Apoptosis

Aminopeptidase Bacterial MMP Apoptosis Antibiotic

Disease Areas

Cancer Infection Neurological, Eye or Ear Disease Inflammation or Immune System Disease Respiratory Disease Digestive System Disease

Pulmonary Disease Pancreatic Cancer Digestive System Cancer Bacterial Infection Pain Digestive System Inflammation

Lung Fibrosis

Natural Products

Disease Research Fields Microorganisms Marine natural products Others

Infection

Reference Standards

Natural Product Standards

Others

Infection Cancer

Cancer Targeted Therapy Cancer Immunotherapy Cancer Metabolism and Metastasis

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Keywords

Actinonin
13434-13-4
(-)-Actinonin
Aminopeptidase
Bacterial
MMP
Apoptosis
Antibiotic
Antibiotics
peptide
deformylase
PDF
reversible
Gram-negative
Gram-positive
antitumor
hmeprin-α
apoptosis
Inhibitor
inhibitor
inhibit

Your Recently Viewed Products

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.5940 mL	12.9702 mL	25.9403 mL	64.8508 mL
5 mM	0.5188 mL	2.5940 mL	5.1881 mL	12.9702 mL
10 mM	0.2594 mL	1.2970 mL	2.5940 mL	6.4851 mL
15 mM	0.1729 mL	0.8647 mL	1.7294 mL	4.3234 mL
20 mM	0.1297 mL	0.6485 mL	1.2970 mL	3.2425 mL
25 mM	0.1038 mL	0.5188 mL	1.0376 mL	2.5940 mL
30 mM	0.0865 mL	0.4323 mL	0.8647 mL	2.1617 mL
40 mM	0.0649 mL	0.3243 mL	0.6485 mL	1.6213 mL
50 mM	0.0519 mL	0.2594 mL	0.5188 mL	1.2970 mL
60 mM	0.0432 mL	0.2162 mL	0.4323 mL	1.0808 mL
80 mM	0.0324 mL	0.1621 mL	0.3243 mL	0.8106 mL
100 mM	0.0259 mL	0.1297 mL	0.2594 mL	0.6485 mL

Actinonin

7 Cited Publications

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Actinonin

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Publications Citing Use of MedChemExpress (MCE) Actinonin

All Aminopeptidase Isoforms

All MMP Isoforms

All Antibiotic Isoforms

Biological Activity

Chemical Information

Publications (7)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Actinonin Related Classifications

HY-113952 Related Classifications