Q-Peptide
Q-Peptide is an angiopoietin-1 derived peptide (QHREDGS). Q-Peptide interacts with β1-integrin, binds to integrins on the surface of osteoblasts, and serves as an acyl donor substrate for Streptomyces mobaraensis transglutaminase. Q-Peptide activates Akt, MAPKp42/44, ILK, ERK1/2, and downregulates caspase-3/7. Q-Peptide inhibits cell apoptosis, enhances cell adhesion and migration, and promotes osteoblast differentiation, bone matrix deposition and mineralization. Q-Peptide can be used in studies related to myocardial infarction, bone regeneration, diabetic wound repair and human induced pluripotent stem cells.
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- No. CAS: 1361235-89-3
- Fòrmula: C31H49N13O14
- Peso molecular:827.80
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Almacenamiento:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Protocolo
- Pureza y Documentación
- Referencias
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Help & FAQs
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Actividad biológica
Descripciòn
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Akt |
p38 MAPK |
ERK1 |
ERK2 |
Caspase 3 |
Caspase-7 |
In Vitro
Q-Peptide (QHREDGS) (5-500 μM; 5 continuous passages, 7 days) improves human induced pluripotent stem cell (BJ1D, 0901B, IMR90) expansion and viability in feeder-dependent culture by reducing caspase-dependent apoptosis, without altering proliferation, while having no significant effect on human embryonic stem cells (H9)[1].
Q-Peptide (50 μM; 5 continuous passages) preserves the in vitro pluripotency of human induced pluripotent stem cells (BJ1D, 0901B)[1].
Q-Peptide (5-500 μM; 48h) significantly increases colony number and size in feeder-free human induced pluripotent stem cells cultured on Matrigel[1].
Q-Peptide (50 μM; 0min-24h) increases integrin-linked kinase (ILK) expression, and increases expression and activation of extracellular signal-regulated kinases 1/2 (ERK1/2) across multiple time points, without affecting AKT activation[1].
Q-Peptide (7.2-29.0 mM; 6h) dose-dependently enhances human induced pluripotent stem cell adhesion to non-adhesive PEG hydrogels, and this interaction is mediated by β1-integrins[1].
Q-Peptide (200 μM; 40 min) potently acts as a substrate for Streptomyces mobaraensis transglutaminase (STG), with a cross-linking conversion yield of 93.0%[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:human induced pluripotent stem cells
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Concentration:50 μM
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Incubation Time:0min, 30min, 1.5h, 2h, 4h, 24h
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Result:Increased ILK protein expression.
Significantly upregulated ERK1/2 expression.
Showed no increase in AKT phosphorylation.
In Vivo
QP-derived functional peptide hydrogel (1% w/v, for local single injection) mediated mesenchymal stem cell transplantation can improve cardiac function (EF and FS), reduce infarct size, collagen content and cardiomyocyte apoptosis in rat myocardial infarction models. This treatment also elevates the survival rate of transplanted cells and promotes angiogenesis. Ctf1 pretreatment can further enhance the therapeutic effects of QP-based hydrogel mediated mesenchymal stem cell transplantation[5].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:BKS.Cg-Dock7m+/+Leprdb/J (db/db) (8-week-old male, leptin-receptor deficient type 2 diabetic, full-thickness 8-mm dorsal excisional wounds)[3]
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Dosage:2.2 nmol
5.9 nmol -
Administration:topical; single application
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Result:Showed faster wound closure starting on day 8.
Reduced normalized wound area significantly by day 14 compared to blank or peptide-free hydrogel controls.
Reduced epithelial gap significantly compared to controls.
Increased re-epithelialization percentage significantly compared to controls.
Enlarged granulation tissue area significantly compared to controls.
Thinned advancing epidermal tongue significantly compared to controls.
Increased total number of blood vessels in the wound significantly compared to blank and peptide-free hydrogel controls.
Was significantly superior to clinically approved collagen dressings in accelerating diabetic wound healing in db/db mice, achieving near-complete wound closure by day 21
- Detection of Apoptosis
- TUNEL staining for apoptotic DNA fragmentation
- Annexin V plus membrane-impermeant dye apoptosis staining
- Human iPSC generation/reprogramming culture
- Cell migration
- Mesenchymal stromal/stem cell osteogenic differentiation
- Apoptosis Solutions
- Research Protocol for Cardiovascular Diseases
- CRISPR-Cas9 editing of human pluripotent stem cells
Chemical Information
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No. CAS 1361235-89-3
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Appearance Solid
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Peso molecular 827.80
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Fòrmula C31H49N13O14
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Color White to off-white
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Sequence
Gln-His-Arg-Glu-Asp-Gly-Ser
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Sequence Shortening
QHREDGS
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvente y solubilidad
In Vitro:
DMSO : 100 mg/mL (120.80 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Pureza y Documentación
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Ficha de datos (280 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Dang LT, et al. Inhibition of apoptosis in human induced pluripotent stem cells during expansion in a defined culture using angiopoietin-1 derived peptide QHREDGS. Biomaterials. 2014;35(27):7786-7799. [Content Brief]
[2]. Feric N, et al. Angiopoietin-1 peptide QHREDGS promotes osteoblast differentiation, bone matrix deposition and mineralization on biomedical materials. Biomater Sci. 2014;2(10):1384-1398. [Content Brief]
[3]. Xiao Y, et al. Diabetic wound regeneration using peptide-modified hydrogels to target re-epithelialization. Proc Natl Acad Sci U S A. 2016;113(40):E5792-E5801. [Content Brief]
[4]. Lee JH, et al. Glutamine (Q)-peptide screening for transglutaminase reaction using mRNA display. Biotechnol Bioeng. 2013;110(2):353-362. [Content Brief]
[5]. Cai H, et al. Self-assembling peptide modified with QHREDGS as a novel delivery system for mesenchymal stem cell transplantation after myocardial infarction. FASEB J. 2019;33(7):8306-8320. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.2080 mL | 6.0401 mL | 12.0802 mL | 30.2005 mL |
| 5 mM | 0.2416 mL | 1.2080 mL | 2.4160 mL | 6.0401 mL | |
| 10 mM | 0.1208 mL | 0.6040 mL | 1.2080 mL | 3.0201 mL | |
| 15 mM | 0.0805 mL | 0.4027 mL | 0.8053 mL | 2.0134 mL | |
| 20 mM | 0.0604 mL | 0.3020 mL | 0.6040 mL | 1.5100 mL | |
| 25 mM | 0.0483 mL | 0.2416 mL | 0.4832 mL | 1.2080 mL | |
| 30 mM | 0.0403 mL | 0.2013 mL | 0.4027 mL | 1.0067 mL | |
| 40 mM | 0.0302 mL | 0.1510 mL | 0.3020 mL | 0.7550 mL | |
| 50 mM | 0.0242 mL | 0.1208 mL | 0.2416 mL | 0.6040 mL | |
| 60 mM | 0.0201 mL | 0.1007 mL | 0.2013 mL | 0.5033 mL | |
| 80 mM | 0.0151 mL | 0.0755 mL | 0.1510 mL | 0.3775 mL | |
| 100 mM | 0.0121 mL | 0.0604 mL | 0.1208 mL | 0.3020 mL |
Q-Peptide Related Classifications
HY-P4866 Related Classifications
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