Cat. No.: HY-B0960

Sulfabenzamide

(Synonyms: N-Sulfanilylbenzamide)

1 Cited Publications

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Based on 1 publication(s) in Google Scholar

Sulfabenzamide (N-Sulfanilylbenzamide) is a sulfonamide antibacterial agent. Sulfabenzamide exhibit antibacterial activity against Staphylococcus aureus (ATCC 25923) and Escherichia coli (ATCC 8739). Sulfabenzamide can promote autophagic cell autophagy in breast cancer cells through p53/ DRAM pathway. Sulfabenzamide increases caspase-3 activity, deactivates PARP1 and DNA-PK, downregulates AKT1 and AKT2. Sulfabenzamide can be used for the researches of breast cancer and bacterial infections.

For research use only. We do not sell to patients.

Purity: 99.68%
CAS No.: 127-71-9
Formula: C₁₃H₁₂N₂O₃S
Molecular Weight:276.32

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

Sulfabenzamide Chemical Structure

CAS No.: 127-71-9

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Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Publications (1)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target^[1]

Caspase 3

Akt1

Akt2

PARP1

All Caspase Isoforms :

All PARP Isoforms :

All Akt Isoforms :

In Vitro

Sulfabenzamide promotes autophagic cell death in T-47D breast cancer cells primarily via induction of autophagy through the p53/DRAM pathway, with attenuation of the AKT/mTOR survival pathway and downregulation of AKT1 and AKT2^[1].
Sulfabenzamide (18 h) and its metal complexes exhibit antibacterial activity against Staphylococcus aureus (ATCC 25923) and Escherichia coli (ATCC 8739); the MIC values of free sulfabenzamide and zinc(II)-sulfabenzamide complex against the two strains are both 1000 ppm^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 127-71-9
Appearance Solid
Molecular Weight 276.32
Formula C₁₃H₁₂N₂O₃S
Color White to off-white
SMILES

O=C(NS(=O)(C1=CC=C(N)C=C1)=O)C2=CC=CC=C2
Synonyms

N-Sulfanilylbenzamide
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (1)

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Int J Biol Macromol

Reticuline isomerase AKR1B1 with aldo-keto reductase activity and detoxification function from the insect Blaps rhynchopetera. [Abstract]

2026 Mar:352:151224. PMID: 41791543

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (361.90 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.6190 mL

18.0950 mL

36.1899 mL

5 mM

0.7238 mL

3.6190 mL

7.2380 mL

10 mM

0.3619 mL

1.8095 mL

3.6190 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

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Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (9.05 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (9.05 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (9.05 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (274 KB)
SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Mohammadpour R, Safarian S, Farahnak S, et al. Sulfabenzamide promotes autophagic cell death in T-47D breast cancer cells through p53/DRAM pathway[J]. J Cell Mol Biol, 2012, 10(1): 41-54.

[2]. Rostamizadeh S, et al. Synthesis of sulfamethoxazole and sulfabenzamide metal complexes; evaluation of their antibacterial activity. Eur J Med Chem. 2019;171:364-371. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.6190 mL	18.0950 mL	36.1899 mL	90.4748 mL
	5 mM	0.7238 mL	3.6190 mL	7.2380 mL	18.0950 mL
	10 mM	0.3619 mL	1.8095 mL	3.6190 mL	9.0475 mL
	15 mM	0.2413 mL	1.2063 mL	2.4127 mL	6.0317 mL
	20 mM	0.1809 mL	0.9047 mL	1.8095 mL	4.5237 mL
	25 mM	0.1448 mL	0.7238 mL	1.4476 mL	3.6190 mL
	30 mM	0.1206 mL	0.6032 mL	1.2063 mL	3.0158 mL
	40 mM	0.0905 mL	0.4524 mL	0.9047 mL	2.2619 mL
	50 mM	0.0724 mL	0.3619 mL	0.7238 mL	1.8095 mL
	60 mM	0.0603 mL	0.3016 mL	0.6032 mL	1.5079 mL
	80 mM	0.0452 mL	0.2262 mL	0.4524 mL	1.1309 mL
	100 mM	0.0362 mL	0.1809 mL	0.3619 mL	0.9047 mL

Sulfabenzamide

1 Cited Publications

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Sulfabenzamide

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Publications Citing Use of MedChemExpress (MCE) Sulfabenzamide

All Caspase Isoforms

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All Akt Isoforms

Biological Activity

Chemical Information

Publications (1)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Sulfabenzamide Related Classifications

HY-B0960 Related Classifications