MO-2097
MO-2097 is a RAF-1/HIF-1α inhibitor. MO-2097 induces RAF-1 destabilization, leading to a reduction in EMT-associated transcription factors and mesenchymal markers. MO-2097 inhibits HIF-1a protein expression mediated by hnRNPA2B1 under hypoxic and mimetic hypoxia. MO-2097 induces mitochondrial ROS, which leads to apoptosis in cells. MO-2097 effectively suppresses colorectal cancer metastasis by inhibiting the RAF/MEK/ERK signaling pathway. MO-2097 attenuates tumor growth in a xenograft HCT116 cell mouse model. MO-2097 can be used for the study of colorectal cancer.
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- CAS No.: 2744300-63-6
- Formule: C19H16O4
- Masse moléculaire:308.33
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
- Pureté et documentation
- Références
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Help & FAQs
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Activité biologique
MO-2097 (10-30 μM, 24-48 h) suppresses cell migration in DLD-1 cells and HCT116 cells[1]. MO-2097 (10-50 μM, 1-6 h) inhibits the RAF/MEK/ERK cascade driven by RAF-1 in DLD-1 cells and HCT116 cells[1]. MO-2097 (10-30 μM, 5-9 days) inhibits the invasiveness of colorectal cancer cells (DLD-1 cells and HCT116 cells) in a 3D tumor spheroid[1]. MO-2097 (30-100 μM, 4 h) inhibits angiogenesis by suppressing tube formation in HUVECs[1]. MO-2097 (25-500 μM, 24 h) exhibits low toxicity, directly binds to hnRNPA2B1, and suppresses HIF-1a expression in HeLa CCL2 cells and HCT116 cells[2]. MO-2097 (25-50 μM, 24 h) inhibits the HIF-1a target gene mRNA (HK1, MRP1, SLC1A5, IL-6, and VEGF) expression levels in HeLa CCL2 cells and HCT116 cells under hypoxic conditions[2]. MO-2097 (12.5-50 μM, 24 h) induces mitochondrial ROS, which leads to apoptosis, as shown by cleaved caspase 3 and 9 in HeLa CCL2 cells[2]. MO-2097 (12.5-50 μM, 7-10 days) induces a specific anticancer effect in the hypoxic zone of the 3D culture spheroid model and 3D-cultured human colon cancer organoids[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:DLD-1 and HCT116 cells
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Concentration:10, 20, 30 μM
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Incubation Time:24, 48 h
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Result:Suppressed cell migration at 86.5% and 77.5% in DLD-1 cells and HCT116 cells, relative to the control, respectively.
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Cell Line:DLD-1 and HCT116 cells
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Concentration:10, 20, 30, 40, 50 μM
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Incubation Time:1, 2, 4, 6 h
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Result:Decreased RAF-1 levels gradually in Cycloheximide (CHX) (HY-12320)-only treated cells.
Prevented RAF-1 accumulation without influencing the proteasomal degradation process.
Reduced the phosphorylation of MEK and ERK.
Decreased the levels of transcription factors Snail, Slug and ZEB1.
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Cell Line:HeLa CCL2 cells
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Concentration:12.5, 25, 50 μM
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Incubation Time:24 h
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Result:Decreased the amount of HIF-1a protein in the nucleus in a dose-dependent manner under hypoxic conditions.
Induced mitochondrial ROS.
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Cell Line:HeLa CCL2 cells and HCT116 cells
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Concentration:25, 50 μM
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Incubation Time:24 h
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Result:Inhibited HK1, MRP1, SLC1A5, IL-6, and VEGF expression levels in HeLa CCL2 cells and HCT116 cells under hypoxic conditions.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Male Balb/C nude mice (6 weeks) xenografted HCT116 cells (2 × 105 cells/mouse)[1]
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Dosage:25, 50 mg/kg
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Administration:i.p. every other day for 15 days
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Result:Reduced the embedded tumor volume by approximately 49 % without any considerable body weight loss.
Decreased HIF-1a expression.
Increased expression of cleaved caspase 3.
Chemical Information
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CAS No. 2744300-63-6
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Masse moléculaire 308.33
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Formule C19H16O4
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SMILES
OC1=CC(C2=CC3=C(C=C4OCC(C)=CCC4=C3)O2)=CC(O)=C1
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Structure Classification
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Initial Source
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureté et documentation
Références
[1]. Kim S, et al. MO-2097 inhibits EMT and angiogenesis in colorectal cancer by targeting RAF/MEK/ERK signaling. Sci Rep. 2025 Jul 1;15(1):21781. [Content Brief]
[2]. Han HJ, et al. HIF-1α inhibition by MO-2097, a novel chiral-free benzofuran targeting hnRNPA2B1. J Adv Res. 2024 Oct;64:67-81. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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