Cat. No.: HY-N5063

Plantainoside D

2 Cited Publications

Customer Review

Based on 2 publication(s) in Google Scholar

Plantainoside D, a phenylethanoid glycosides, is a IKK-β inhibitor with diverse biological activities. Plantainoside D shows inhibitory activity of angiotensin-converting enzyme (ACE) with an IC₅₀ of 2.17 mM. Plantainoside D significantly reduces the release of glutamate from nerve terminals in the cerebral cortex of rats by inhibiting the voltage-dependent calcium channel (VDCCs) and protein kinase C (PKC) signaling cascade. Plantainoside D significantly alleviates cell apoptosis by inhibiting the generation of ROS and the activation of NF-κB. Plantainoside D significantly improves acute lung injury (ALI) induced by sepsis by regulating the Sirt3/NLRP3 signaling pathway. Plantainoside D can be used for the study of neuroprotection, antioxidant, anti-inflammation, antihypertension.

For research use only. We do not sell to patients.

Purity: 99.15%
CAS No.: 147331-98-4
Formula: C₂₉H₃₆O₁₆
Molecular Weight:640.59

Storage:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Plantainoside D Chemical Structure

CAS No.: 147331-98-4

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (2)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target^[1]^[2]^[5]^[6]

IKK-β

Calcium Channel

NF-κB

NLRP3

ACE

2.17 mM (IC₅₀)

PKC

All IKK Isoforms :

All Calcium Channel Isoforms :

All PKC Isoforms :

All NF-κB Isoforms :

All Sirtuin Isoforms :

All NOD-like Receptor (NLR) Isoforms :

In Vitro

Plantainoside D (0-50 μM, 10 min) significantly inhibits the release of glutamate induced by 4-aminopyridine (4-AP) (HY-B0604) via vesicle exocytosis pathway with an IC₅₀ of 32 μM in rat synaptosome^[1].
Plantainoside D (30 μM, 10 min) reduces the Ca²⁺ influx induced by 4-AP through N-type calcium channels, and does not alter the membrane potential in rat synaptosome^[1].
Plantainoside D (30 μM, 20 min) acts through PKC-α/SNAP-25 phosphorylation as its key downstream mechanism in rat synaptosome^[1].
Plantainoside D (5 μM, 24 h) significantly improves sepsis induced ALI by regulation of Sirt3/NLRP3 pathway in MLE-12 cells^[2].
Plantainoside D (1-20 μg/mL, 1-4 h) significantly reduces the apoptosis induced by Doxorubicin (ADR) (HY-15142A) by inhibiting the generation of ROS and the activation of NF-κB^[3].
Plantainoside D exhibits weak inhibitory effects of CYP1A2 (IC₅₀ = 12.83 μM), CYP2D6 (IC₅₀ = 8.39 μM) and CYP3A4 (IC₅₀ = 14.66 μM)^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:

Rat cerebral cortical synaptosomes
Concentration:

30 μM
Incubation Time:

20 min
Result:

Reduced the phosphorylation of PKC-α (from 239.2% to 54.1%) and SNAP-25 (from 228.9% to 89.7%) induced by 4-AP.

Cell Viability Assay^[2]

Cell Line:

MLE-12 cells induced by LPS (HY-D1056)
Concentration:

5 μM
Incubation Time:

24 h
Result:

Increased cell vitality, reduced ROS and MDA.

Apoptosis Analysis^[3]

Cell Line:

H9c2 cell with ADR
Concentration:

1, 5, 10, 20 μg/mL
Incubation Time:

1 h
Result:

Reduced the cell mortality rate with 20 μg/mL pretreatment.
Reduced apoptotic morphology (nuclear enrichment, fragmentation).
Increased Bcl-2/Bax ratio and inhibited caspase-3.

Western Blot Analysis^[3]

Cell Line:

H9c2 cell with ADR
Concentration:

2 μM
Incubation Time:

15 min, 30 min, 1h, 2h, 4h
Result:

Abrogated the activation of NF-κB.
The glycoside reduced the degradation of IkBα in ADR-treated cells.

In Vivo

Plantainoside D (50 mg/kg, i.p., once daily for 7 days) significantly improves sepsis induced ALI by regulation of Sirt3/NLRP3 pathway in mice^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

LPS-induced ALI model established in ICR WT male mice (6 weeks, 16-18 g)^[2]
Dosage:

50 mg/kg
Administration:

Intraperitoneal injection (i.p.), once daily for 7 days
Result:

Significantly decreased the levels of w/d, ROS and MPO.
Significantly decreased MDA and increased SOD and GSH-px in serum and lung tissues.
Significantly restored oxidative stress and neutrophil infiltration.
Significantly decreased the levels of cytokines TNF-α, IL-1β, IL-6, NLRP3, ASC and caspase-1.

Chemical Information

CAS No. 147331-98-4
Appearance Solid
Molecular Weight 640.59
Formula C₂₉H₃₆O₁₆
Color White to light yellow
SMILES

O[C@H]([C@H]([C@@H]([C@@H](COC(/C=C/C1=CC=C(O)C(O)=C1)=O)O2)O)O[C@H]3[C@@H]([C@H]([C@@H]([C@@H](CO)O3)O)O)O)[C@@H]2OCCC4=CC=C(O)C(O)=C4
Structure Classification
- Phenylpropanoids
- Simple Phenylpropanols
- Phenols
- Polyphenols
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (2)

All IPIn Vivo Efficacy StudyHistological Imaging/StainingIFELISA

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Journal Impact Factor

Redox Biol

Extracellular vesicle-packaged GBP2 from macrophages aggravates sepsis-induced acute lung injury by promoting ferroptosis in pulmonary vascular endothelial cells. [Abstract]

2025 Mar 25:82:103614. PMID: 40156957

Plantainoside D purchased from MedChemExpress. Usage Cited in: Redox Biol. 2025 Mar 25:82:103614. [Abstract]

Representative images of immunoblotting of GPX4 protein and its ubiquitination levels in HPMECs co-cultured with SMφ-EVs, with or without Plantainoside D (PD) (10 μM) treatment.

Plantainoside D purchased from MedChemExpress. Usage Cited in: Redox Biol. 2025 Mar 25:82:103614. [Abstract]

Survival rate of mice treated with or without CLP following administration of AAV-shGbp2 or Plantainoside D PD (30 mg/kg, i.v.) treatment.

Plantainoside D purchased from MedChemExpress. Usage Cited in: Redox Biol. 2025 Mar 25:82:103614. [Abstract]

Representative images of Evans blue staining and HE staining of mouse lungs treated with Plantainoside D PD (30 mg/kg, i.v.).

Plantainoside D purchased from MedChemExpress. Usage Cited in: Redox Biol. 2025 Mar 25:82:103614. [Abstract]

Representative images and quantification of Tunel staining of mouse lungs treated with Plantainoside D PD (30 mg/kg, i.v.).

Plantainoside D purchased from MedChemExpress. Usage Cited in: Redox Biol. 2025 Mar 25:82:103614. [Abstract]

Quantification of IL-6, TNF-α and IL-1β in mouse lungs treated with Plantainoside D PD (30 mg/kg, i.v.).
Biochem Pharmacol

KCMF1 regulates HRI ubiquitination to inhibit the integrated stress response in ovarian cancer. [Abstract]

2025 Dec 13:245:117634. PMID: 41391693

Solvent & Solubility

In Vitro:

DMSO : 250 mg/mL (390.27 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.5611 mL

7.8053 mL

15.6106 mL

5 mM

0.3122 mL

1.5611 mL

3.1221 mL

10 mM

0.1561 mL

0.7805 mL

1.5611 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (3.25 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (3.25 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (283 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Chiu KM, et al. Plantainoside D Reduces Depolarization-Evoked Glutamate Release from Rat Cerebral Cortical Synaptosomes. Molecules. 2023 Jan 30;28(3):1313. [Content Brief]

[2]. Wang J, et al. Sirt3 regulates NLRP3 and participates in the effects of plantainoside D on acute lung injury sepsis. Aging (Albany NY). 2023 Apr 5;15(14):6710-6720. [Content Brief]

[3]. Kim DS, et al. Plantainoside D protects adriamycin-induced apoptosis in H9c2 cardiac muscle cells via the inhibition of ROS generation and NF-kappaB activation. Life Sci. 2007 Jan 2;80(4):314-23. [Content Brief]

[4]. Zhou J, et al. Human liver microsomes study on the inhibitory effect of plantainoside D on the activity of cytochrome P450 activity. BMC Complement Med Ther. 2022 Jul 23;22(1):197. [Content Brief]

[5]. Geng F, Yang L, Chou G, Wang Z. Bioguided isolation of angiotensin-converting enzyme inhibitors from the seeds of Plantago asiatica L. Phytother Res. 2010;24(7):1088-1094. [Content Brief]

[6]. Zhou P, Hua F, Wang X, Huang JL. Therapeutic potential of IKK-β inhibitors from natural phenolics for inflammation in cardiovascular diseases. Inflammopharmacology. 2020;28(1):19-37. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.5611 mL	7.8053 mL	15.6106 mL	39.0265 mL
	5 mM	0.3122 mL	1.5611 mL	3.1221 mL	7.8053 mL
	10 mM	0.1561 mL	0.7805 mL	1.5611 mL	3.9027 mL
	15 mM	0.1041 mL	0.5204 mL	1.0407 mL	2.6018 mL
	20 mM	0.0781 mL	0.3903 mL	0.7805 mL	1.9513 mL
	25 mM	0.0624 mL	0.3122 mL	0.6244 mL	1.5611 mL
	30 mM	0.0520 mL	0.2602 mL	0.5204 mL	1.3009 mL
	40 mM	0.0390 mL	0.1951 mL	0.3903 mL	0.9757 mL
	50 mM	0.0312 mL	0.1561 mL	0.3122 mL	0.7805 mL
	60 mM	0.0260 mL	0.1301 mL	0.2602 mL	0.6504 mL
	80 mM	0.0195 mL	0.0976 mL	0.1951 mL	0.4878 mL
	100 mM	0.0156 mL	0.0781 mL	0.1561 mL	0.3903 mL

Keywords

Plantainoside D

2 Cited Publications

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Plantainoside D

Apoptosis Compound Library

Cell Cycle/DNA Damage Compound Library

Epigenetics Compound Library

Immunology/Inflammation Compound Library

Kinase Inhibitor Library

Membrane Transporter/Ion Channel Compound Library

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