MAPK-IN-5
MAPK-IN-5 is a potent MAPK inhibitor with an IC50 of 1.35 μM against HeLa cells. MAPK-IN-5 inhibits HeLa cell proliferation by inducing ROS-mediated DNA damage and mitochondrial apoptosis via the MAPK pathway. MAPK-IN-5 significantly inhibits colony formation, reduces the number of live cells, suppresses cell migration, and causes cell cycle arrest in the G2/M phase in HeLa cells. MAPK-IN-5 can be used for the study of cervical cancer.
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- CAS. Nr.: 2579683-39-7
- Formel: C30H29F3N6O4
- Molecular Weight:594.58
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Speicherung:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biologische Aktivität
Beschreibung
IC50 & Target
[1]|
p38 |
Caspase-3 |
Caspase-9 |
Bcl-2 |
PARP-1 |
In Vitro
MAPK-IN-5 (Compound 3h) (5-10 μM, 48 h) significantly inhibits the colony formation of HeLa cells and increases the number of dead cells[1].
MAPK-IN-5 (5-10 μM, 0-48 h) has a pronounced inhibitory effect on the migration of HeLa cells[1].
MAPK-IN-5 (5-10 μM, 24 h) induces G2/M phase arrest in HeLa cells[1].
MAPK-IN-5 (5-10 μM, 48 h) induces apoptosis in HeLa cells, and the activation of the mitochondrial apoptosis pathway is involved[1].
MAPK-IN-5 (5-10 μM, 48 h) promotes ROS generation, triggers DNA damage and modulates the MAPK signaling pathway in HeLa cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HeLa cells
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Concentration:5 and 10 μM
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Incubation Time:48 h
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Result:Gradually decreased the number of live cells, while the number of dead cells increased.
Dose-dependently increased the intracellular ROS level.
Increased the fluorescence intensity of γ-H2AX in the nuclei of HeLa cells.
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Cell Line:HeLa cells
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Concentration:5 and 10 μM
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Incubation Time:24 h
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Result:Gradually decreased the proportion of cells in the G1 phase, while the proportion of cells in the G2/M phase significantly accumulated.
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Cell Line:HeLa cells
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Concentration:5 and 10 μM
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Incubation Time:0, 24, 48 h
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Result:Reduced the migration rate of HeLa cells was to 20.75% at 10 μM.
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Cell Line:HeLa cells
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Concentration:5 and 10 μM
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Incubation Time:48 h
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Result:Increased the total apoptosis rate of HeLa cells by 37.63% at 10 μM.
Caused cell shrinkage, intensified nuclear staining, and even apoptotic features such as nuclear condensation and fragmentation were observed.
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Cell Line:HeLa cells
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Concentration:5 and 10 μM
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Incubation Time:48 h
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Result:Decreased the levels of Bcl-2, Caspase-3, Caspase-9, and PARP-1.
Increased the levels of Bax and Cleaved PARP-1.
Dose-dependently inhibited the levels of phosphorylated ERK1/2 (p-ERK1/2) and phosphorylated JNK (p-JNK), while upregulating the phosphorylation of p38.
Had no effect on the total protein levels of ERK1/2, JNK, and p38.
- Cell migration
- Somatic Cell Culture
- Detection of Apoptosis
- TUNEL staining for apoptotic DNA fragmentation
- Flow cytometric DNA-content cell-cycle staining
- Chemotaxis Gradient Chamber Assay 1
- Under-Agarose Cell Migration Assay
- Transwell/Boyden Chamber Migration Assay
- Apoptosis Solutions
- 3D Collagen/Hydrogel Matrix Migration Assay
- Soft Agar Colony Formation Assay
- MTT Cell Proliferation Assay
- Cell-Exclusion Zone Migration Assay
- Chemotaxis Gradient Chamber Assay 2
- Genotoxicity/Mutagenicity Study
- Scratch/Wound-Healing Migration Assay
- Annexin V plus membrane-impermeant dye apoptosis staining
- BrdU Incorporation Assay
- ROS/oxidative-stress fluorescent staining
- Neural Crest/Neuronal Cell Migration Explant Assay
- CFSE Dye Dilution Proliferation Assay
- Dye-dilution cell tracking and proliferation staining
- CCK-8/WST-8 Cell Proliferation Assay
- Protocol for Cell Cycle
- Cell Counting-Based Growth Curve Assay
- Colony Formation (Clonogenic) Assay
- Cell Viability Determination by MTT Colorimetric Assay
- EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
- Ki-67 Immunostaining Proliferation Assay
- PCNA Immunodetection Proliferation Assay
- Protocol for Cell Counting and Cell Density Analysis
Chemical Information
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CAS. Nr. 2579683-39-7
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Molecular Weight 594.58
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Formel C30H29F3N6O4
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SMILES
COCCOC(C=C1N=CN=C2NC3=CC(C(N=N4)=CN4CC5=CC=C(C(F)(F)F)C=C5)=CC=C3)=C(C=C12)OCCOC
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Please store the product under the recommended conditions in the Certificate of Analysis.
Reinheit & Dokumentation
Verweise
Calculators
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
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