Cat. No.: HY-126741

Azadirachtin

3 Cited Publications

Customer Review

Based on 3 publication(s) in Google Scholar

Azadirachtin is an oral active triterpenoid compound with anticancer, antimalarial, anti-inflammatory, and insecticidal activities. Azadirachtin induces cell apoptosis through the mitochondrial pathway (by inhibiting Bcl-2/Bax ratio or activating Apaf-1 and caspase-3) or through death receptors (by inhibiting TNFR activation). Additionally, Azadirachtin exerts its anti-inflammatory effects by inhibiting NF-кB signaling pathway activation, and it exhibits insecticidal activity by inducing apoptosis in insect cells.

For research use only. We do not sell to patients.

Purity: 99.06%
CAS No.: 11141-17-6
Formula: C₃₅H₄₄O₁₆
Molecular Weight:720.71

Storage:

-20°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Azadirachtin Chemical Structure

CAS No.: 11141-17-6

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
- Clinical Trial
Chemical Information
Publications (3)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

Caspase 3

All NF-κB Isoforms :

All Caspase Isoforms :

All Bcl-2 Family Isoforms :

All Parasite Isoforms :

In Vitro

Azadirachtin (3-24 μM, 6 h) exerts its anti-inflammatory effects in Retinoic acid (HY-14649)-induced A549 (lung carcinoma) cells by inhibiting the NF-κB signaling pathway^[3].
Azadirachtin (135 μM, 24 h) induces cell cycle arrest and mitochondria-mediated apoptosis in human cervical cancer (HeLa) cells^[5].
Azadirachtin (50 μg/mL, 22-24 h) inhibits the early sporogonic development of Plasmodium berghei and blocks its transmission effect^[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[3]

Cell Line:

Retinoic acid (HY-14649) -induced human lung carcinoma (A549) cells
Concentration:

0, 3, 6, 12, 24 μM
Incubation Time:

6 h
Result:

Inhibited Retinoic acid (HY-14649)-induced NF-κB-dependent gene (ICAM1 (intercellular adhesion molecule 1) and cox2) expression

In Vivo

Azadirachtin (10 μg/kg, i.g., once daily, 3 days a week for 14 weeks) carcinogenesis in the DMBA (HY-W011845)-induced hamster buccal pouch (HBP) carcinoma model by modulating xenobiotic metabolism enzymes, DNA damage, antioxidants, invasion, and angiogenesis.^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

DMBA (HY-W011845)-induced hamster buccal pouch (HBP) carcinoma model^[4]
Dosage:

10 μg/kg
Administration:

Intragastric administration (i.g.), once daily, 3 days a week for 14 weeks
Result:

Significantly decreased the expression of CYP1A1, CYP1B1 and 8-OHdG and up-regulated RECK expression.
Significantly enhanced GSH and the activities of all the antioxidant enzymes.
Decreased phase I enzyme activities and elevated phase II enzyme activities in the pouch.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS No. 11141-17-6
Appearance Solid
Molecular Weight 720.71
Formula C₃₅H₄₄O₁₆
Color White to light yellow
SMILES

C[C@@]1([C@@]([C@@]2(O)C(OC)=O)([H])[C@]([C@H](C[C@H]3OC(C)=O)OC(/C(C)=C/C)=O)(CO2)[C@@]4([H])[C@](OC[C@@]43C(OC)=O)([H])[C@H]1O)[C@]([C@@H](O[C@]5([H])OC=C6)C[C@H]7[C@]56O)(O8)[C@]78C
Structure Classification
- Terpenoids
- Triterpenes
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (3)

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Journal Impact Factor

J Agric Food Chem

Azadirachtin Induces Fat Body Apoptosis by Suppressing Caspase- 8 in the Fall Armyworm, Spodoptera frugiperda. [Abstract]

2024 Sep 4;72(35):19323-19332. PMID: 39174876
J Agric Food Chem

Azadirachtin Inhibits Nuclear Receptor HR3 in the Prothoracic Gland to Block Larval Ecdysis in the Fall Armyworm, Spodoptera frugiperda. [Abstract]

2023 Oct 25;71(42):15497-15505. PMID: 37843053
Insect Sci

Genetic and pharmacological inhibition of the Kir2A channel in diamondback moth exhibits ovicidal activity. [Abstract]

2026 May 11. PMID: 42116718

Solvent & Solubility

In Vitro:

DMSO : ≥ 100 mg/mL (138.75 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.3875 mL

6.9376 mL

13.8752 mL

5 mM

0.2775 mL

1.3875 mL

2.7750 mL

10 mM

0.1388 mL

0.6938 mL

1.3875 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (3.47 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (3.47 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (3.47 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (289 KB)
SDS (537 KB)
- English - EN (537 KB)
- Français - FR (537 KB)
- Deutsch - DE (537 KB)
- Norwegian - NO (537 KB)
- Español - ES (537 KB)
- Swedish - SV (537 KB)
- Italian - IT (537 KB)
- Korean - KR (537 KB)
- Portuguese - PT (537 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Shu B, Jia J, Zhang J, Sethuraman V, Yi X, Zhong G. DnaJ homolog subfamily A member1 (DnaJ1) is a newly discovered anti-apoptotic protein regulated by azadirachtin in Sf9 cells. BMC Genomics. 2018;19(1):413. Published 2018 May 29. [Content Brief]

[2]. Fernandes SR, et al. Chemistry, bioactivities, extraction and analysis of azadirachtin: State-of-the-art. Fitoterapia. 2019 Apr;134:141-150. [Content Brief]

[3]. Thoh M, et al. Azadirachtin interacts with retinoic acid receptors and inhibits retinoic acid-mediated biological responses. J Biol Chem. 2011 Feb 11;286(6):4690-702. doi: 10.1074/jbc.M110.169334. Epub 2010 Dec 2. Retraction in: J Biol Chem. 2013 Mar 22;288(12):8563. [Content Brief]

[4]. Priyadarsini RV, et al. The neem limonoids azadirachtin and nimbolide inhibit hamster cheek pouch carcinogenesis by modulating xenobiotic-metabolizing enzymes, DNA damage, antioxidants, invasion and angiogenesis. Free Radic Res. 2009 May;43(5):492-504. [Content Brief]

[5]. Priyadarsini RV, et al. The neem limonoids azadirachtin and nimbolide induce cell cycle arrest and mitochondria-mediated apoptosis in human cervical cancer (HeLa) cells. [Content Brief]

[6]. Tapanelli S, et al. Transmission blocking effects of neem (Azadirachta indica) seed kernel limonoids on Plasmodium berghei early sporogonic development. Fitoterapia. 2016 Oct;114:122-126. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.3875 mL	6.9376 mL	13.8752 mL	34.6880 mL
	5 mM	0.2775 mL	1.3875 mL	2.7750 mL	6.9376 mL
	10 mM	0.1388 mL	0.6938 mL	1.3875 mL	3.4688 mL
	15 mM	0.0925 mL	0.4625 mL	0.9250 mL	2.3125 mL
	20 mM	0.0694 mL	0.3469 mL	0.6938 mL	1.7344 mL
	25 mM	0.0555 mL	0.2775 mL	0.5550 mL	1.3875 mL
	30 mM	0.0463 mL	0.2313 mL	0.4625 mL	1.1563 mL
	40 mM	0.0347 mL	0.1734 mL	0.3469 mL	0.8672 mL
	50 mM	0.0278 mL	0.1388 mL	0.2775 mL	0.6938 mL
	60 mM	0.0231 mL	0.1156 mL	0.2313 mL	0.5781 mL
	80 mM	0.0173 mL	0.0867 mL	0.1734 mL	0.4336 mL
	100 mM	0.0139 mL	0.0694 mL	0.1388 mL	0.3469 mL

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Azadirachtin

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Publications Citing Use of MedChemExpress (MCE) Azadirachtin

All NF-κB Isoforms

All Caspase Isoforms

All Bcl-2 Family Isoforms

All Parasite Isoforms

Biological Activity

Chemical Information

Publications (3)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Azadirachtin Related Classifications

HY-126741 Related Classifications