Cat. No.: HY-P11935

RR-171

RR-171 is an amino acid polypeptide and an inhibitor of the Wnt signaling pathway. RR-171 reduces the expression levels of Wnt-1, GSK3β and β-catenin. RR-171 induces apoptosis (Apoptosis) in pancreatic cancer cells, which is characterized by an increased Bax/Bcl-2 ratio, activation of Caspase-3/7/9, and increased Cleaved-PARP; this pro-apoptotic effect can be partially reversed by Z-VAD-FMK (HY-16658B). RR-171 also induces pyroptosis (Pyroptosis) in pancreatic cancer cells, which is manifested by activation of the NLRP-3 inflammasome, activation of Caspase-1, cleavage of GSDMD, upregulation of IL-1β and IL-18, and increased LDH release; this pro-pyroptotic effect can be partially reversed by VX-765 (HY-13205). RR-171 inhibits the viability, proliferation and colony formation of pancreatic cancer cells, with low cytotoxicity against normal cells. RR-171 downregulates the expression of Ki-67 and PCNA in tumor tissues in vivo, with favorable biosafety. RR-171 can be used in studies related to pancreatic cancer.

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CAS No.: 2410535-57-6
화학식: C₉₂H₁₇₇N₄₁O₁₈
분자량:2145.65

보관:

Please store the product under the recommended conditions in the Certificate of Analysis.

맞춤형 펩타이드 합성

RR-171 Chemical Structure

CAS No.: 2410535-57-6

Amount: USD 0.00

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- 제품 설명
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
순도&문서
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

제품 설명

IC₅₀ & Target^[1]

Caspase-1

Caspase-3

Caspase-9

Caspase-7

IL-1β

IL-18

All Wnt Isoforms :

All NOD-like Receptor (NLR) Isoforms :

All Caspase Isoforms :

All Bcl-2 Family Isoforms :

All GSK-3 Isoforms :

All Interleukin Related Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
Capan-2	IC50	24.70 μM	Inhibition of cell viability against human Capan-2 pancreatic cancer cells incubated for 24 hrs by CCK-8 assay. Inhibition of cell viability against human Capan-2 pancreatic cancer cells incubated for 24 hrs by CCK-8 assay.	40229415
BXPC-3	IC50	15.71 μM	Inhibition of cell viability against human Bxpc-3 pancreatic cancer cells incubated for 24 hrs by CCK-8 assay. Inhibition of cell viability against human Bxpc-3 pancreatic cancer cells incubated for 24 hrs by CCK-8 assay.	40229415
PANC-1	IC50	75.68 μM	Inhibition of cell viability against human Panc-1 pancreatic cancer cells incubated for 24 hrs by CCK-8 assay. Inhibition of cell viability against human Panc-1 pancreatic cancer cells incubated for 24 hrs by CCK-8 assay.	40229415

In Vitro

RR-171 (0-200 µM; 24 h) inhibits the viability of Panc-1, Capan-2 and Bxpc-3 pancreatic cancer cells in a dose-dependent manner, with IC₅₀ values of 75.68 µM, 24.70 µM and 15.71 µM, respectively; whereas it exerts weak inhibitory effects on HPNE normal pancreatic epithelial cells (IC₅₀ = 144.50 µM)^[1].
RR-171 (0-20 µM; 24 h) inhibits colony formation of Capan-2 and Bxpc-3 pancreatic cancer cells in a dose-dependent manner^[1].
RR-171 (0-20 µM; 48 h) inhibits the proliferation of Capan-2 and Bxpc-3 pancreatic cancer cells in a dose-dependent manner^[1].
RR-171 (0-20 µM; 24 h) reduces the expression of Wnt-1, GSK3β and β-catenin in Capan-2 and Bxpc-3 pancreatic cancer cells in a dose-dependent manner, thereby inhibiting the canonical Wnt signaling pathway^[1].
RR-171 (0-20 µM; 24 h) regulates the expression of apoptosis-related proteins in Capan-2 and Bxpc-3 pancreatic cancer cells in a dose-dependent manner; it increases the levels of Bax and cleaved PARP, while decreasing the levels of Bcl-2, caspase-3, caspase-7 and caspase-9^[1].
Treatment of Capan-2 pancreatic cancer cells with RR-171 (20 µM; 24 h) alters their global gene expression, thereby enriching apoptosis-related pathways and inhibiting the canonical Wnt signaling pathway^[1].
RR-171 (0-20 µM; 24 h) induces caspase-mediated apoptosis in Capan-2 and Bxpc-3 pancreatic cancer cells in a dose-dependent manner, and this effect is partially reversed by Z-VAD-FMK (HY-16658B)^[1].
Treatment of Capan-2 pancreatic cancer cells with RR-171 (5-20 µM; 24 h) induces dose-dependent morphological changes consistent with pyroptosis, including reduced cell junctions, membrane blebbing and cell rupture^[1].
RR-171 (0-20 µM; 24 h) induces dose-dependent LDH release in Capan-2 and Bxpc-3 pancreatic cancer cells. This effect is significantly reversed by the pyroptosis inhibitor VX-765, but cannot be completely reversed by the pan-caspase inhibitor Z-VAD-FMK^[1].
RR-171 (0-20 µM; 24 h) dose-dependently upregulates the expression of pyroptosis-related proteins (NLRP-3, GSDMD, caspase-1, IL-1β, IL-18) in Capan-2 and Bxpc-3 pancreatic cancer cells, and this effect is reversed by VX-765 (HY-13205)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

Panc-1, Capan-2, Bxpc-3, HPNE
Concentration:

0, 0.5, 1, 5, 10, 20, 40, 80, 100 and 200 µM
Incubation Time:

24 h
Result:

Inhibited pancreatic cancer cell viability in a dose-dependent manner, with reduced inhibitory effects on normal pancreatic epithelial cells.
Achieved IC₅₀ values of 24.70 µM for Capan-2 cells, 15.71 µM for Bxpc-3 cells, 5075.68 µM for Panc-1 cells, and 144.50 µM for HPNE cells at 24 h.

Cell Proliferation Assay^[1]

Cell Line:

Capan-2, Bxpc-3
Concentration:

5, 10 and 20 µM
Incubation Time:

48 h
Result:

Distinctly suppressed the proliferation of Capan-2 and Bxpc-3 cells in a dose-dependent manner, as indicated by reduced EdU-positive staining relative to control cells.

Western Blot Analysis^[1]

Cell Line:

Capan-2, Bxpc-3
Concentration:

5, 10 and 20 µM
Incubation Time:

24 h
Result:

Decreased the expression levels of Wnt-1, GSK3β, and β-catenin in a dose-dependent manner in both Capan-2 and Bxpc-3 cells, confirming inhibition of the canonical Wnt signaling pathway.\nDecreased antiapoptotic protein Bcl-2 expression, while increased proapoptotic protein Bax expression in a dose-dependent manner as concentration increased.
Reduced levels of caspase-3, caspase-7, and caspase-9, while increased cleaved-PARP levels, indicating activation of the caspase-mediated apoptosis pathway.

Apoptosis Analysis^[1]

Cell Line:

Capan-2, Bxpc-3
Concentration:

5, 10 and 20 µM
Incubation Time:

24 h
Result:

Distinctively increased the percentage of apoptotic Capan-2 and Bxpc-3 cells in a dose-dependent manner.
Significantly reduced the apoptosis ratio in both cell lines when co-treated with Z-VAD-FMK, confirming a caspase-dependent mechanism.

Western Blot Analysis^[1]

Cell Line:

Capan-2, Bxpc-3
Concentration:

5, 10 and 20 µM
Incubation Time:

24 h
Result:

Increased levels of NLRP-3, GSDMD, caspase-1, IL-1β, and IL-18 in a dose-dependent manner in both cell lines.
Decreased the elevated levels of these pyroptosis-related proteins when co-treated with VX-765.

In Vivo

RR-171 (20 mg/kg; i.p.; every 3 days; three weeks) significantly reduces pancreatic cancer xenograft tumor volume and weight, suppresses tumor cell proliferation markers Ki-67 and PCNA, and shows no significant organ toxicity in nude mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

BALB/c nude (female, 5-6 weeks old, subcutaneous pancreatic cancer xenograft model)^[1]
Dosage:

20 mg/kg
Administration:

i.p.; every 3 days; three weeks
Result:

Reduced tumor volume significantly starting on Day 17 post-cell injection.
Lowered final tumor weight significantly compared to controls.
Showed no significant difference in mouse body weight between groups.
Decreased average density of Ki-67 and PCNA in tumor tissues significantly.
Exhibited no significant organ toxicity in lung, heart, liver, spleen, and kidney tissues.

Chemical Information

CAS No. 2410535-57-6
분자량 2145.65
화학식 C₉₂H₁₇₇N₄₁O₁₈
Sequence

Arg-Arg-Arg-Arg-Leu-Val-Ala-Gly-Val-Leu-Val-Leu-Leu-Arg-Arg-Arg-Arg
Sequence Shortening

RRRRLVAGVLVLLRRRR
선적

Room temperature in continental US; may vary elsewhere.
보관

Please store the product under the recommended conditions in the Certificate of Analysis.

순도&문서

Handling Instructions (2659 KB)

References

[1]. Chen W, et al. A novel peptide RR-171 derived from human umbilical cord serum induces apoptosis and pyroptosis in pancreatic cancer cells. Scientific reports. 2025 Apr 14;15(1):12819.

Calculators

몰농도 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

농도 희석 계산기

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Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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RR-171

All Wnt Isoforms

All NOD-like Receptor (NLR) Isoforms

All Caspase Isoforms

All Bcl-2 Family Isoforms

All GSK-3 Isoforms

All Interleukin Related Isoforms

Biological Activity

Chemical Information

순도&문서

References

Calculators

Help & FAQs

RR-171 Related Classifications

HY-P11935 Related Classifications

Bioz