2. Epigenetics Apoptosis Metabolic Enzyme/Protease
  3. YTHDF Apoptosis MMP Caspase
  4. SKLB-Y13

SKLB-Y13 is a selective YTHDF1 inhibitor with an IC50 of 0.76 μM and a KD of 5.097 μM. SKLB-Y13 binds to the Tyr397 and Trp470 residues in the m6A-binding pocket of YTHDF1, disrupting the interaction between YTHDF1 and m6A-modified mRNA. SKLB-Y13 inhibits cancer cell proliferation and promotes apoptosis. SKLB-Y13 is applicable to breast cancer-related research.

For research use only. We do not sell to patients.

SKLB-Y13

SKLB-Y13 Chemical Structure

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
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Customer Review

Based on 1 publication(s) in Google Scholar

SKLB-Y13 Related Antibodies

Top Publications Citing Use of Products

View All YTHDF Isoform Specific Products:

View All Isoforms
YTHDF1 YTHDF3 YTHDF2 YTHDC1 YTHDC2

View All MMP Isoform Specific Products:

View All Isoforms
MMP-1 MMP-2 MMP-3 MMP-8 MMP-9 MMP-13 MMP-14 MMP-17 ADAM10 ADAM17 MMP-7 MMP-12 MMP-10 MMP ADAM8 MMP-19

View All Caspase Isoform Specific Products:

View All Isoforms
Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

SKLB-Y13 is a selective YTHDF1 inhibitor with an IC50 of 0.76 μM and a KD of 5.097 μM. SKLB-Y13 binds to the Tyr397 and Trp470 residues in the m6A-binding pocket of YTHDF1, disrupting the interaction between YTHDF1 and m6A-modified mRNA. SKLB-Y13 inhibits cancer cell proliferation and promotes apoptosis. SKLB-Y13 is applicable to breast cancer-related research[1].

IC50 & Target[1]

YTHDF1

0.76 μM (IC50)

YTHDF2

4.27 μM (IC50)

YTHDF3

6.17 μM (IC50)

In Vitro

SKLB-Y13 upregulates m6A levels in MDA-MB-231 cells in a YTHDF1-dependent manner[1].
SKLB-Y13 (0-20 μM) inhibits the viability of MDA-MB-231 and MDA-MB-468 breast cancer cells[1].
SKLB-Y13 significantly inhibits the invasion of MDA-MB-231 and MDA-MB-468 breast cancer cells, and reduces the expression of metastasis-related proteins (MMP9, MMP2, snail and vimentin)[1].
SKLB-Y13 (0-10 μM) induces concentration-dependent apoptosis in MDA-MB-231 and MDA-MB-468 breast cancer cells, accompanied by upregulated expression of Cleaved-Caspase-3 and Cleaved-Parp1[1].
SKLB-Y13 (0-10 μM) dose-dependently downregulates the protein expression of PRPF6, MAP2K6, c-FOS and STK4 in MDA-MB-231 breast cancer cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

SKLB-Y13 Related Antibodies

Cell Viability Assay[1]

Cell Line: MDA-MB-231, MDA-MB-468, MCF-10A
Concentration: 0, 5, 10, 15, 20 μM
Incubation Time: /
Result: Inhibited the viability of MDA-MB-231 (IC50 = 6.05 μM) and MDA-MB-468 (IC50 = 6.34 μM) breast cancer cells, with minimal effect on normal MCF-10A cells.
In Vivo

SKLB-Y13 (10-40 mg/kg; i.p.; once daily; for 16 consecutive days) dose-dependently inhibits the growth of MDA-MB-231 breast cancer xenografts[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: BABL/c nude mice treated MDA-MB-231[1]
Dosage: 10 mg/kg; 20 mg/kg; 40 mg/kg
Administration: i.p.; daily; 16 days
Result: Significantly reduced tumor volume and tumor weight compared to controls.
Reduced Ki-67-positive cells in a dose-dependent manner.
Increased Cleaved-Caspase-3 and Cleaved-Parp1 levels in a dose-dependent manner.
Significantly downregulated PRPF6, MAP2K6, c-FOS, and STK4 protein expression.
Caused no significant changes in mouse body weight or pathological changes in major organs.
Molecular Weight

514.43

Formula

C23H21Cl2N7OS
Appearance

Solid

Color

Light yellow to yellow

SMILES

ClC1=CN=C(N=C1NC2=C(C3=C(S2)CN(CC3)CC4=CC=CC=C4Cl)C(N)=O)C5=CC=NN5C
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (194.39 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.9439 mL 9.7195 mL 19.4390 mL
5 mM 0.3888 mL 1.9439 mL 3.8878 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (4.86 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.9439 mL 9.7195 mL 19.4390 mL 48.5975 mL
5 mM 0.3888 mL 1.9439 mL 3.8878 mL 9.7195 mL
10 mM 0.1944 mL 0.9719 mL 1.9439 mL 4.8597 mL
15 mM 0.1296 mL 0.6480 mL 1.2959 mL 3.2398 mL
20 mM 0.0972 mL 0.4860 mL 0.9719 mL 2.4299 mL
25 mM 0.0778 mL 0.3888 mL 0.7776 mL 1.9439 mL
30 mM 0.0648 mL 0.3240 mL 0.6480 mL 1.6199 mL
40 mM 0.0486 mL 0.2430 mL 0.4860 mL 1.2149 mL
50 mM 0.0389 mL 0.1944 mL 0.3888 mL 0.9719 mL
60 mM 0.0324 mL 0.1620 mL 0.3240 mL 0.8100 mL
80 mM 0.0243 mL 0.1215 mL 0.2430 mL 0.6075 mL
100 mM 0.0194 mL 0.0972 mL 0.1944 mL 0.4860 mL
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SKLB-Y13 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

SKLB-Y13YTHDFApoptosisMMPCaspase(YTH) domain-containing family proteins (YTHDF)Matrix metalloproteinasesCleaved-Caspase-3Cleaved-Parp1YTHDF1YTH family proteinsMDA-MB-468PRPF6MDA-MB-231MCF-10Abreast cancerm6A-modified mRNAInhibitorinhibitorinhibit

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