Porphyra 334
Based on 1 Customer Validation
Porphyra 334 is a carnosine-like amino acid and a natural photoprotective agent and antioxidant. Porphyra-334 exerts its photoprotective effects by scavenging ROS, inhibiting the expression and activity of MMP-1/8, and promoting the synthesis of collagen and elastin. Porphyra 334 effectively inhibits linoleic acid oxidation induced by alkyl radicals (AAPH) and singlet oxygen. Porphyra 334 has anti-obesity potential by inhibiting the expression of PPARγ2 and C/EBPα. Porphyra 334 protects cells against UV-induced DNA damage and apoptosis by inhibiting the activation of caspase-3.
For research use only. We do not sell to patients.
- Purity: 99.58%
- CAS No.: 70579-26-9
- Formula: C14H22N2O8
- Molecular Weight:346.33
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
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PPARγ2 |
MMP-1 |
MMP-8 |
Caspase 3 |
Porphyra-334 (6.25-200 μM; 24 h) shows no cytotoxicity against 3T3-L1 preadipocytes at the highest concentration of 200 μM[1].
Porphyra-334 (0.1-1.0 μM) inhibits lipid droplet accumulation in mature 3T3-L1 adipocytes in a dose-dependent manner[1].
Porphyra-334 (0.1-1.0 μM; 5 days) dose-dependently and significantly reduces the mRNA expression levels of PPARγ2, C/EBPα, adiponectin, and leptin in 3T3-L1 cells[1].
Porphyra-334 (0.1 mg/mL; 24-96 h) restores cell proliferation to approximately 88% of that in the unirradiated control group by reducing caspase-3 activation, DNA fragmentation, and the number of apoptotic cells, thereby alleviating UV-induced apoptosis in HaCaT cells[2].
Porphyra 334 (10-40 μM; 24 h) protects human skin fibroblasts (CCD-986sk) exposed to long-wave ultraviolet (UVA) radiation from photoaging by scavenging reactive oxygen species (ROS), inhibiting the expression of MMP-1/MMP-8 and elastase activity, and increasing the levels of procollagen, type I collagen and elastin[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:3T3-L1 preadipocytes
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Concentration:6.25, 12.5, 25, 50, 200 μM
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Incubation Time:24 h
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Result:Showed no cytotoxic effect on 3T3-L1 cells at concentrations <200 μM, with cell viability comparable to the control group; Observed no morphological changes similar to the control.
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Cell Line:3T3-L1 cells
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Concentration:0.1 and 1.0 μM
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Incubation Time:5 days
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Result:Significantly reduced the mRNA expression of peroxisome proliferator-activated receptor γ2 (PPARγ2), CCAAT/enhancer-binding protein α (C/EBPα), adiponectin, and leptin in a dose-dependent manner.
Chemical Information
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CAS No. 70579-26-9
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Appearance Solid
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Molecular Weight 346.33
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Formula C14H22N2O8
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Color White to off-white
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SMILES
C[C@@H](O)[C@@H](C(O)=O)/N=C1C(OC)=C(C[C@](O)(C/1)CO)NCC(O)=O
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Structure Classification
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Initial Source
Palythoa tuberculosa
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (288.74 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.22 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (7.22 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (276 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[2]. Suh SS, et al. Porphyra-334, a mycosporine-like amino acid, attenuates UV-induced apoptosis in HaCaT cells. Acta Pharm. 2017;67(2):257-264. [Content Brief]
[3]. Ryu J, et al. Protective effect of porphyra-334 on UVA-induced photoaging in human skin fibroblasts. Int J Mol Med. 2014;34(3):796-803. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 2.8874 mL | 14.4371 mL | 28.8742 mL | 72.1855 mL |
| 5 mM | 0.5775 mL | 2.8874 mL | 5.7748 mL | 14.4371 mL | |
| 10 mM | 0.2887 mL | 1.4437 mL | 2.8874 mL | 7.2185 mL | |
| 15 mM | 0.1925 mL | 0.9625 mL | 1.9249 mL | 4.8124 mL | |
| 20 mM | 0.1444 mL | 0.7219 mL | 1.4437 mL | 3.6093 mL | |
| 25 mM | 0.1155 mL | 0.5775 mL | 1.1550 mL | 2.8874 mL | |
| 30 mM | 0.0962 mL | 0.4812 mL | 0.9625 mL | 2.4062 mL | |
| 40 mM | 0.0722 mL | 0.3609 mL | 0.7219 mL | 1.8046 mL | |
| 50 mM | 0.0577 mL | 0.2887 mL | 0.5775 mL | 1.4437 mL | |
| 60 mM | 0.0481 mL | 0.2406 mL | 0.4812 mL | 1.2031 mL | |
| 80 mM | 0.0361 mL | 0.1805 mL | 0.3609 mL | 0.9023 mL | |
| 100 mM | 0.0289 mL | 0.1444 mL | 0.2887 mL | 0.7219 mL |
Porphyra 334 Related Classifications
HY-126956 Related Classifications
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- Porphyra 334
- 70579-26-9
- Porphyra334
- Porphyra-334
- Reactive Oxygen Species (ROS)
- MMP
- Collagen
- PPAR
- DNA/RNA Synthesis
- Apoptosis
- Caspase
- CCAAT/enhancer-binding protein alpha (C/EBPα)
- 3T3-L1 cells
- laver
- human skin fibroblasts
- HaCaT cells
- peroxisome proliferator-activated receptor gamma 2 (PPARγ2)
- CCD-986sk
- leptin
- matrix metalloproteinase-1 (MMP-1)
- adiponectin (ADIPOQ)
- Inhibitor
- inhibitor
- inhibit