Echinoside A
Echinoside A is a saponin. Echinoside A can be isolated from sea cucumber. Echinoside A inhibits the catalytic activity of Top2α, reduces the noncovalent binding of Top2α to DNA. Echinoside A activates Caspase-3 and induces PARP cleavage. Echinoside A induces Apoptosis. Echinoside A has anticancer activity against prostate cancer, hepatocellular carcinoma, and S-180 sarcoma. Echinoside A exhibits antifungal activity against a variety of fungi, with a minimum growth inhibitory concentration range of 3.12 to 50.0 μg/mL, including potent activity against Aspergillus and Penicillium species.
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- No. CAS: 75410-53-6
- Fòrmula: C54H87NaO26S
- Peso molecular:1207.31
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Almacenamiento:
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Actividad biológica
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Chemical Information
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Actividad biológica
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topoisomerase II alpha |
Caspase-3 |
Echinoside A (0.80-3.98 μM; 6-24 h) potently inhibits the proliferation of HepG2 cells in a dose-dependent manner, and reduces cell viability by 54.7% at 2.70 μM for 12 h[1].
Echinoside A (1.35-2.70 μM; 12 h) induces apoptosis in HepG2 cells in a dose-dependent manner in vitro[1].
Echinoside A (1.35-2.70 μM; 12 h) regulates the expression of cell cycle-related genes in HepG2 cells, upregulates the expression of p16 and p21, downregulates the expression of cyclin D1, but has no effect on p27[1].
Echinoside A (1.35-2.70 μM; 12 h) exerts no significant effect on the expression of NF-κB p65 protein in HepG2 cells[1].
Echinoside A (0.25-2 μM; 3 h for DNA fragmentation) induces concentration-dependent apoptosis and DNA fragmentation in human leukemia HL-60 cells[2].
Echinoside A exhibits antifungal activity against a variety of fungi, with a minimum growth inhibitory concentration range of 3.12 to 50.0 μg/mL, including potent activity against *Aspergillus* and *Penicillium* species[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:human hepatocellular liver carcinoma HepG2 cells
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Concentration:1.35, 2.70 μM
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Incubation Time:12 h
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Result:Showed slightly increased percentages of early and late apoptotic cells compared to control cells.
Induced visible perinuclear chromatin condensation as bright green patches or fragments in cells treated with 2.70 μM.\nResulted in 18.1% total apoptotic cells (13.2% early apoptotic, 4.9% late apoptotic) at 1.35 μM for 12 h.
Resulted in 62.2% total apoptotic cells at 2.70 μM for 12 h.
Induced apoptosis in a dose-dependent manner.
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Cell Line:human hepatocellular liver carcinoma HepG2 cells
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Concentration:1.35, 2.70 μM
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Incubation Time:12 h
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Result:Increased the population of HepG2 cells in the G0/G1 phase from 67.9% to 75.2% at 2.70 μM for 12 h.
Increased the sub-G0/G1 cell population (indicative of apoptosis) from 1.83% to 21.21% at 2.70 μM for 12 h.
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Cell Line:human hepatocellular liver carcinoma HepG2 cells
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Concentration:1.35, 2.70 μM
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Incubation Time:12 h
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Result:Activated caspase-3 and induced PARP cleavage.\nHad no significant inhibitory effect on NF-κB p65 protein expression.
Echinoside A (1.5-3.0 mg/kg/day; i.v.; daily; 7 days) reduces S-180 sarcoma growth in KM mice[2].
Echinoside A (2.6-5.2 mg/kg; i.v.; weekly; 4 weeks) suppresses PC-3 prostate carcinoma xenograft growth in nude mice with T/C % values of 53.7% and 45.1%, respectively[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:BALB/c (male, 17-22g, subcutaneous implantation of H22 hepatocarcinoma cells)[1]
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Dosage:0.5 mg/kg; 2.5 mg/kg
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Administration:i.p.; daily; 10 days
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Result:Reduced mean tumour weight to 1.53 ± 0.36 g, corresponding to a 33.8% tumour growth inhibition rate (P < 0.01).
Reduced mean tumour weight to 1.16 ± 0.22 g, corresponding to a 49.8% tumour growth inhibition rate (P < 0.01).
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Animal Model:KM mice (female, 7 weeks old, 18-22 g, specific pathogen-free, S-180 sarcoma model via s.c. inoculation)[2]
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Dosage:1.5 mg/kg/day; 3.0 mg/kg/day
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Administration:i.v.; daily; 7 days
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Result:Achieved a tumor growth inhibition rate of 52.0% at 1.5 mg/kg.
Achieved a tumor growth inhibition rate of 80.0% at 3 mg/kg.
Chemical Information
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No. CAS 75410-53-6
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Peso molecular 1207.31
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Fòrmula C54H87NaO26S
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SMILES
C[C@]12[C@]3([C@H](C=C4[C@@]2([H])CC[C@]5([H])[C@@]4(CC[C@@H](C5(C)C)O[C@H]6[C@@H]([C@H]([C@@H](CO6)OS(=O)(O[Na])=O)O)O[C@H]7[C@@H]([C@H]([C@@H]([C@H](O7)C)O[C@H]8[C@@H]([C@H]([C@@H]([C@H](O8)CO)O)O[C@H]9[C@@H]([C@H]([C@@H]([C@H](O9)CO)O)OC)O)O)O)O)C)O)[C@@]([C@@](C)(OC3=O)CCCC(C)C)(CC1)O
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Structure Classification
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Initial Source
Echinoside A was isolated from sea cucumber
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureza y Documentación
Referencias
[1]. Zhao Q, et al. In vitro and in vivo anti-tumour activities of echinoside A and ds-echinoside A from Pearsonothuria graeffei. J Sci Food Agric. 2012;92(4):965-974. [Content Brief]
[2]. Li M, et al. Echinoside A, a new marine-derived anticancer saponin, targets topoisomerase2alpha by unique interference with its DNA binding and catalytic cycle. Ann Oncol. 2010 Mar;21(3):597-607. [Content Brief]
[3]. Kitagawa I, et al. Marine natural products. XIV. Structures of echinosides A and B, antifungal lanostane-oligosides from the sea cucumber Actinopyga echinites (Jaeger). Chem Pharm Bull (Tokyo). 1985 Dec;33(12):5214-24. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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