ODN 2007
Based on 1 Customer Validation
ODN 2007, a class B CpG ODN (oligodeoxynucleotide), is a Toll-like receptor (TLR) ligand. ODN 2007 can be used as an immunomodulator, vaccine adjuvant, and enhance immune responses in mammals, fish, and humans. ODN 2007 sequence: 5'-TCGTCGTTGTCGTTTTGTCGTT-3'.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度: 97.86%
- CAS 番号: 455348-63-7
- 分子量:6852.50
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保管条件:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
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溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
ODN 2007 (1 or 5 μg/mL, 3-18 h) induces significantly chicken macrophage interferon IFN-γ and IFN-β expression[1].
ODN 2007 (10 μg/mL, 0.5-12 h) can increase levels of phosphorylation of ERK2 and AKT, stimulate the production of NO[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:MQ-NCSU cells (a chicken macrophage cell line)
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Concentration:1 μg/mL, 5 μg/mL
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Incubation Time:3 h, 12 h, 18 h
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Result:Significantly stimulated an increase in transcriptional expression of IFN-γ after 3 hours regardless of the dose.
Increased the mRNA expression of IL-1β at 5 μg/mL high dose regardless of the time point.
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Cell Line:HD11, a replication-deficient avian leukemia virus MC29-transformed macrophage-like cell line
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Concentration:10 μg/mL
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Incubation Time:0.5 h, 1 h, 3 h, 6 h, 12 h
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Result:Resulted in a significant increase in ERK2 and AKT phosphorylation levels and increased IFN-γ, IL-6 and MIP-3α mRNA levels.
Stimulated an increase in the level of NO.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Zebrafish infected with Vibrio traumaticus FJ03-X2[2]
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Dosage:1 μg
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Administration:Intraperitoneal injection; once
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Result:Showed a cumulative mortality rate of 15.0% in treated group while the control group reached 42.1%.
Showed a significant decrease in the expression levels of all intestinal immune-related genes such as TNF、IFNg1-2、IL-1β、IL-10, especially IL-1β decreased by 99.88%.
化学情報
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CAS 番号 455348-63-7
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性状 Solid
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分子量 6852.50
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Color White to off-white
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SMILES
[DNA, d(P-thio)(T-C-G-T-C-G-T-T-G-T-C-G-T-T-T-T-G-T-C-G-T-T)]
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配列
DNA, d(P-thio)(T-C-G-T-C-G-T-T-G-T-C-G-T-T-T-T-G-T-C-G-T-T)
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
溶剤 & 溶解度
H2O : 20 mg/mL (2.92 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
純度とドキュメンテーション
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データシート (271 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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取扱説明書 (2242 KB)
参考文献
[1]. Tamiru N Alkie, et al. Characterization of Innate Responses Induced by PLGA Encapsulated- and Soluble TLR Ligands In Vitro and In Vivo in Chickens. PLoS One. 2017 Jan 3;12(1):e0169154. [Content Brief]
[3]. Audesh Bhat, et al. Role of Hsp90 in CpG ODN mediated immunostimulation in avian macrophages. Mol Immunol. 2010 Mar;47(6):1337-46. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 0.1459 mL | 0.7297 mL | 1.4593 mL | 3.6483 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
ODN 2007 関連分類
HY-150734 関連分類
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