Cat. No.: HY-N0674

Dehydrocorydaline

Name: Dehydrocorydaline
Brand: MedChemExpress
SKU: HY-N0674
Price: 56.00 USD
Availability: InStock
Rating: 5 (47 reviews)

(Synonyms: 13-Methylpalmatine)

47 Cited Publications

고객리뷰

Based on 47 publication(s) in Google Scholar

Dehydrocorydaline (13-Methylpalmatine) is an alkaloid that regulates protein expression of Bax, Bcl-2; activates caspase-7, caspase-8, and inactivates PARP. Dehydrocorydaline elevates p38 MAPK activation. Anti-inflammatory and anti-cancer activities. Dehydrocorydaline shows strong anti-malarial effects (IC₅₀=38 nM), and low cytotoxicity (cell viability > 90%) using P. falciparum 3D7 strain.

연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.

Purity : 99.16%
CAS No.: 30045-16-0
화학식: C₂₂H₂₄NO₄⁺
분자량:366.43

보관:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Dehydrocorydaline Chemical Structure

CAS No.: 30045-16-0

사이즈

가격

재고

수량

Amount: USD 0.00

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- 제품 설명
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (47)
용액&용해도
- In Vitro
- In Vivo
Protocol (10)
순도&문서
References
Help & FAQs
Classifications
Bioz

Biological Activity

제품 설명

Dehydrocorydaline (13-Methylpalmatine) is an alkaloid that regulates protein expression of Bax, Bcl-2; activates caspase-7, caspase-8, and inactivates PARP^[1]. Dehydrocorydaline elevates p38 MAPK activation. Anti-inflammatory and anti-cancer activities^[2]. Dehydrocorydaline shows strong anti-malarial effects (IC₅₀=38 nM), and low cytotoxicity (cell viability > 90%) using P. falciparum 3D7 strain^[3].

IC₅₀ & Target

^[1]

Plasmodium

Bcl-2

Bax

Caspase-7

Caspase-8

PARP

All Bcl-2 Family Isoforms :

All Caspase Isoforms :

All PARP Isoforms :

All p38 MAPK Isoforms :

All Parasite Isoforms :

In Vitro

Dehydrocorydaline (0-200 μM) treatment significantly inhibits the growth of MCF-7 cells in a dose-dependent manner. The cell viability is decreased by approximate 40% after 24 h of 200 μM Dehydrocorydaline^[1].
Dehydrocorydaline (0-200 μM)dose-dependently increases Bax protein expression and decreases Bcl-2 protein expression^[1].
Dehydrocorydaline (0-200 μM)induces activation of caspase-7,-8 and the cleavage of PARP without affecting caspase-9^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.

In Vivo

Dehydrocorydaline manifests a low acute toxicity with an LD₅₀ of about 277.5±19.0 mg/kg body weight in mice following oral administration and 21.1±1.4 mg/kg for intraperitoneal injection^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 30045-16-0
Appearance Solid
분자량 366.43
화학식 C₂₂H₂₄NO₄⁺
Color Light yellow to yellow
SMILES

CC1=C(C=CC(OC)=C2OC)C2=C[N+]3=C1C4=CC(OC)=C(OC)C=C4CC3
Synonyms

13-Methylpalmatine
Structure Classification
- Alkaloids
- Isoquinoline Alkaloids
Initial Source
선적

Room temperature in continental US; may vary elsewhere.

보관

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (47)

All Histological Imaging/StainingRT-PCRIFCell Proliferation/Viability AssayWB

Sort by:

Journal Impact Factor

J Nanobiotechnology

A fibrin gel-loaded Gouqi-derived nanovesicle (GqDNV) repairs the heart after myocardial infarction by inhibiting p38 MAPK/NF-κB p65 pathway. [Abstract]

2025 Jul 22;23(1):535. PMID: 40696398
J Clin Invest

Myostatin regulates energy homeostasis through autocrine- and paracrine-mediated microenvironment communications. [Abstract]

2024 Jun 18;134(16):e178303. PMID: 38889010
Phytomedicine

The role of daurisoline treatment in hepatocellular carcinoma: Inhibiting vasculogenic mimicry formation and enhancing sensitivity to sorafenib. [Abstract]

2021 Nov:92:153740. PMID: 34600176
Mater Today Bio

Dual targeted gene delivery strategy mediated by GalNAc-modified lipid nanoparticles enhances liver regeneration through specific knockdown of MKK4. [Abstract]

2026 Mar 24:38:103059. PMID: 41970250
New Phytol

LbSakA-mediated phosphorylation of the scaffolding protein LbNoxR in the ectomycorrhizal basidiomycete Laccaria bicolor regulates NADPH oxidase activity, ROS accumulation and symbiosis development. [Abstract]

2024 Jul;243(1):381-397. PMID: 38741469
Int J Biol Macromol

Deoxycholic acid inhibits ASFV replication by inhibiting MAPK signaling pathway. [Abstract]

2024 May;266(Pt 1):130939. PMID: 38493816
Phytother Res

Dehydrocorydaline Promotes STAT3/Bcl-2 Axis-Mediated Macrophage Efferocytosis to Attenuate Apoptosis in Atherosclerotic Plaques. [Abstract]

2026 May;40(5):2473-2490. PMID: 41699991
J Ethnopharmacol

Dehydrocorydaline attenuates myocardial ischemia-reperfusion injury via the FoXO signalling pathway: A multimodal study based on network pharmacology, molecular docking, and experimental study. [Abstract]

2025 Jan 30;337(Pt 1):118738. PMID: 39222757
J Agric Food Chem

Sulforaphene Inhibits Periodontitis through Regulating Macrophage Polarization via Upregulating Dendritic Cell Immunoreceptor. [Abstract]

2023 Oct 25;71(42):15538-15552. PMID: 37823224
Ecotoxicol Environ Saf

Dibutyl phthalate promotes central precocious puberty through primordial follicle activation by downregulating BMP15. [Abstract]

2025 Jul 15:300:118460. PMID: 40472697
Biochem Pharmacol

STM2457 decreases m6A methylation to reduce cisplatin-induced ototoxicity via MAPK signaling. [Abstract]

2025 May:235:116820. PMID: 39983847
Biochem Pharmacol

Ursodeoxycholic acid alleviates fat embolism syndrome-induced acute lung injury by inhibiting the p38 MAPK/NF-κB signalling pathway through FXR. [Abstract]

2024 Dec;230(Pt 1):116574. PMID: 39396648
Mol Metab

TRPC6 governs brown adipose thermogenesis via a BMPR2-p38 MAPK signaling axis. [Abstract]

2026 Apr:106:102341. PMID: 41747880
Eur J Pharmacol

Therapeutic potential of oral edaravone in diabetic endothelial dysfunction through NF-κB and p38 pathway inhibition. [Abstract]

2026 Jan 20:1012:178500. PMID: 41421410
Respir Res

Dehydrocorydaline attenuates bleomycin-induced pulmonary fibrosis by inhibiting fibroblast activation. [Abstract]

2025 Apr 12;26(1):136. PMID: 40221718

Dehydrocorydaline purchased from MedChemExpress. Usage Cited in: Respir Res. 2025 Apr 12;26(1):136. [Abstract]

Images of mice lung tissue slices stained with H&E, Masson, and Sirius Red treated with L-DHC (Dehydrocorydaline) 5 mg/kg, H-DHC: 10 mg/kg.

Dehydrocorydaline purchased from MedChemExpress. Usage Cited in: Respir Res. 2025 Apr 12;26(1):136. [Abstract]

RT-qPCR results for Col1a1 and Fn1 expression levels of mice lung tissue treated with L-DHC (Dehydrocorydaline) 5 mg/kg, H-DHC: 10 mg/kg.

Dehydrocorydaline purchased from MedChemExpress. Usage Cited in: Respir Res. 2025 Apr 12;26(1):136. [Abstract]

Collagen 1 and Fibronectin of mice lung tissues are analyzed using Western blot treated with L-DHC (Dehydrocorydaline) 5 mg/kg, H-DHC: 10 mg/kg.

Dehydrocorydaline purchased from MedChemExpress. Usage Cited in: Respir Res. 2025 Apr 12;26(1):136. [Abstract]

Immunofluorescence images of α-SMA, Collagen 1, and Fibronectin in mice lung tissue slices treated with L-DHC (Dehydrocorydaline) 5 mg/kg, H-DHC: 10 mg/kg.

Dehydrocorydaline purchased from MedChemExpress. Usage Cited in: Respir Res. 2025 Apr 12;26(1):136. [Abstract]

CCK8 assay to determine the impact of Dehydrocorydaline (DHC) (5, 10, 30, 50, 80, 100, 150 μM) on HPFs viability.
Eur J Pharmacol

Eriodictyol inhibits glioblastoma migration and invasion by reversing EMT via downregulation of the P38 MAPK/GSK-3β/ZEB1 pathway. [Abstract]

2021 Jun 5:900:174069. PMID: 33811837
Int Immunopharmacol

MCPIP1 modulates host antifungal immunity and exacerbates lethal Candida albicans infection. [Abstract]

2026 Jul 15:181:116715. PMID: 42066549
Int Immunopharmacol

Salvianolic acid a inhibits neuroinflammation and ameliorates Alzheimer's disease pathology via the p38 MAPK/NF-κB pathway based on network pharmacology and experimental validation. [Abstract]

2026 Jun 1:178:116594. PMID: 41930874
Int Immunopharmacol

Eupatilin suppresses osteoclastogenesis and periodontal bone loss by inhibiting the MAPKs/Siglec-15 pathway. [Abstract]

2024 Sep 30:139:112720. PMID: 39047450
Bone Joint Res

Melatonin induces RAW264.7 cell apoptosis via the BMAL1/ROS/MAPK-p38 pathway to improve postmenopausal osteoporosis. [Abstract]

2023 Nov 1;12(11):677-690. PMID: 37907083
Arthritis Res Ther

Roles of VWA1 and Rac2 in compensatory and decompensatory responses via FGF9/FGFR3 and p38 MAPK signaling pathways in condylar chondrocytes under distinct mechanical stress. [Abstract]

2025 May 31;27(1):118. PMID: 40450276
Front Pharmacol

Inhibition of ASIC1a-Mediated ERS Improves the Activation of HSCs and Copper Transport Under Copper Load. [Abstract]

2021 May 31:12:653272. PMID: 34135753
J Inflamm Res

The Effects of Adoptively Transferred IL-23/IL-18-Polarized Neutrophils on Tumor and Collagen-Induced Arthritis in Mice. [Abstract]

2021 Sep 16;14:4669-4686. PMID: 34557012
FASEB J

USP44 Regulates Chemoresistance Induced by ROS and the MAPK/NF-κB Pathway Through the Stabilization of ITGB4 in Gastric Cancer. [Abstract]

2025 Aug 31;39(16):e70945. PMID: 40824171
J Nutr

A-Type Cinnamon Procyanidin Oligomers Protect Against 1-Methyl-4-Phenyl-1,2,3,6-Tetrahydropyridine-Induced Neurotoxicity in Mice Through Inhibiting the P38 Mitogen-Activated Protein Kinase/P53/BCL-2 Associated X Protein Signaling Pathway. [Abstract]

2020 Jul 1;150(7):1731-1737. PMID: 32386222
Front Biosci (Landmark Ed)

NELFCD Promotes Colon Cancer Progression by Regulating the DUSP2-p38 Axis. [Abstract]

2025 Apr 25;30(4):25221. PMID: 40302325
J Food Sci

Curcumin Alleviates DON-Induced Intestinal Epithelial Barrier Disruption by Improving Ribotoxic Stress-Associated p38 Pathway-Mediated TJ Injury, Apoptosis, and Cell Cycle Arrest. [Abstract]

2025 Apr;90(4):e70217. PMID: 40271829
J Cell Physiol

STRIP2 silencing inhibits vascular smooth muscle cell proliferation and migration via P38-AKT-MMP-2 signaling pathway. [Abstract]

2019 Dec;234(12):22463-22476. PMID: 31093976
Genomics

MiR-100-5p as a biomarker in hand-arm vibration disease: Mediating pathogenesis through angiogenesis suppression. [Abstract]

2025 Jul;117(4):111067. PMID: 40460899
Mol Carcinog

ITGB3-enriched extracellular vesicles mediate the formation of osteoclastic pre-metastatic niche to promote lung adenocarcinoma bone metastasis. [Abstract]

2024 Nov;63(11):2190-2204. PMID: 39136603
Andrology

Expression of IL-33 in rodent testes and its role in Leydig cell steroidogenesis and aging. [Abstract]

2025 Jun 16. PMID: 40521800
Tissue Cell

Placental mesenchymal stem cells-secreted proenkephalin suppresses the p38 MAPK signaling to block hyperproliferation of keloid fibroblasts. [Abstract]

2023 Dec:85:102218. PMID: 37913601
Exp Eye Res

Embryonic stem cell extracellular vesicles reverse the senescence of retinal pigment epithelial cells by the p38MAPK pathway. [Abstract]

2023 Feb:227:109365. PMID: 36577484
BMC Musculoskelet Disord

Knockdown of PAR2 alleviates cancer-induced bone pain by inhibiting the activation of astrocytes and the ERK pathway. [Abstract]

2022 May 30;23(1):514. PMID: 35637468
Infect Genet Evol

Knockdown of GBP1 inhibits BCG-induced apoptosis in macrophage RAW 264.7 cells via p38/JNK pathway. [Abstract]

2022 Jan:97:105158. PMID: 34826624
J Mol Histol

Nogo-A-Δ20/EphA4 interaction antagonizes apoptosis of neural stem cells by integrating p38 and JNK MAPK signaling. [Abstract]

2021 Jun;52(3):521-537. PMID: 33555537
Biochem Biophys Res Commun

Deficiency of unc-51 like kinase 1 (Ulk1) protects against mice traumatic brain injury (TBI) by suppression of p38 and JNK pathway. [Abstract]

2018 Sep 5;503(2):467-473. PMID: 29680658

Dehydrocorydaline purchased from MedChemExpress. Usage Cited in: Biochem Biophys Res Commun. 2018 Sep 5;503(2):467-473. [Abstract]

The increase of Bcl-2 and decrease of Bax, cleaved Caspase-3 and PARP induced by Ulk1- inhibition in LPS-exposed AST are abrogated by the Dehydrocorydaline chloride (DHC) or Anisomycin (ANS) pre-treatment
Biochem Biophys Res Commun

NFATc3 deficiency protects against high fat diet (HFD)-induced hypothalamus inflammation and apoptosis via p38 and JNK suppression. [Abstract]

2018 May 23;499(4):743-750. PMID: 29596828

Dehydrocorydaline purchased from MedChemExpress. Usage Cited in: Biochem Biophys Res Commun. 2018 May 23;499(4):743-750. [Abstract]

Astrocytes are cultured with p38 and JNK activator of DHC and ANS, respectively, at the indicated concentrations for 24 h. Then, all cells are harvested for western blot analysis of p-p38 and p-JNK. Astrocytes are pretreated with DHC (500 nM) or ANS (10 mM) for 24 h, followed by Fru (5 mM) for an additional 24 h.
Eur J Histochem

Adipose-derived stem cells promote the recovery of intestinal barrier function by inhibiting the p38 MAPK signaling pathway. [Abstract]

2025 Jan 21;69(1). PMID: 39836101
J Clin Neurosci

Effects of p38 MAPK signaling pathway on cognitive function and recovery of neuronal function after hypoxic-ischemic brain injury in newborn rats. [Abstract]

2020 Aug:78:365-370. PMID: 32360159
J Cardiothorac Surg

Aconitine promotes ROS-activated P38/MAPK/Nrf2 pathway to inhibit autophagy and promote myocardial injury. [Abstract]

2024 Dec 20;19(1):665. PMID: 39707526
bioRxiv

2026 May 7.
Bioengineered

Deleted in lymphocytic leukemia 2 induces retinoic acid receptor beta promoter methylation and mitogen activated kinase-like protein activation to enhance viability and mobility of colorectal cancer cells. [Abstract]

2022 May;13(5):12847-12862. PMID: 35611845
Aging (Albany NY)

Short-term high-fat diet favors the appearances of apoptosis and gliosis by activation of ERK1/2/p38MAPK pathways in brain. [Abstract]

2021 Oct 7;13(19):23133-23148. PMID: 34620734
Comput Math Methods Med

Efficacy of Low Molecular Heparin on Preeclampsia by Inhibiting Apoptosis of Trophoblasts via the p38MAPK Signaling Pathway. [Abstract]

2021 Aug 2:2021:3337514. PMID: 34394705
Aging (Albany NY)

CircRNA_30032 promotes renal fibrosis in UUO model mice via miRNA-96-5p/HBEGF/KRAS axis. [Abstract]

2021 May 11;13(9):12780-12799. PMID: 33973871
Research Square Preprint

2020 Dec.

용액&용해도

In Vitro:

DMSO : 25 mg/mL (68.23 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.7290 mL

13.6452 mL

27.2903 mL

5 mM

0.5458 mL

2.7290 mL

5.4581 mL

10 mM

0.2729 mL

1.3645 mL

2.7290 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

몰농도 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

농도 희석 계산기

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (6.82 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (6.82 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Protocol

All Molecular Biology Cell Biology Signal Pathway Related

RNA extraction experimental
By lysing cells, releasing RNA, and removing impurities such as proteins and DNA, high-purity RNA products are finally obtained. The commonly used traditional method is the guanidine isothiocyanate/phenol/chloroform method (Trizol), which is suitable for a variety of animal materials including animal tissues, microorganisms, cultured cells, etc., and most plant materials.
Western Blot
Western blotting (WB) is a commonly used experimental method in molecular biology, biochemistry, and immunogenetics for identifying and quantifying target proteins. It combines gel electrophoresis with immunoassay, enabling researchers to analyze protein expression, post-translational modifications, and molecular weight.
Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
Mammalian live/dead viability and cytotoxicity staining
Live/dead viability and cytotoxicity staining assays are based on the simultaneous detection of intracellular esterase activity in metabolically active (viable) cells and membrane integrity loss in non-viable cells. In commonly used dual-staining approaches, membrane-permeant fluorogenic substrates are converted by intracellular esterases into fluorescent products in live cells, while impermeant DNA-binding dyes selectively enter cells with compromised plasma membranes and label nucleic acids in dead or dying cells, enabling discrimination between viable and non-viable populations by fluorescence microscopy or flow cytometry.
Apoptosis Solutions
Apoptosis is a regulated, generally non-lytic cell-death pathway that removes unwanted, damaged, infected, or abnormal cells through coordinated morphological changes, caspase activation, DNA fragmentation, and membrane remodeling. The intrinsic apoptosis pathway is controlled mainly by mitochondrial outer membrane permeabilization, BCL-2 family proteins, cytochrome c release, apoptosome formation, caspase-9 activation, and downstream executioner caspase-3/7 activation. The extrinsic apoptosis pathway is initiated by death receptors such as Fas, TNFR, and TRAIL receptors, which recruit adaptor proteins and activate caspase-8 before engaging executioner caspases or mitochondrial amplification through BID cleavage. Apoptosis is linked to many phenotypes, including cancer cell killing, tissue homeostasis, immune regulation, neurodegeneration, infection response, and treatment-induced cytotoxicity; unresolved questions include how apoptosis interacts with necroptosis, pyroptosis, ferroptos
CCK-8/WST-8 Cell Proliferation Assay
The CCK-8/WST-8 assay is based on the reduction of the water-soluble tetrazolium salt WST-8 to a water-soluble formazan product by cellular dehydrogenases in metabolically active cells, where the generated formazan amount is proportional to the number of living cells and is quantified by measuring absorbance in the visible range, providing a colorimetric readout for cell viability and proliferation assessment. This class of tetrazolium-based assays improves upon earlier MTT-based systems by producing a water-soluble formazan, eliminating the need for organic solubilization steps and enabling direct spectrophotometric measurement in culture medium.
Research Protocol for Inflammation-related Diseases
The NLRP3 inflammasome is a cytosolic innate immune signaling platform that integrates priming signals and danger-signal activation to promote caspase-1 activation, maturation of IL-1β and IL-18, and gasdermin D-mediated pyroptotic cell death. The core experimental logic is to determine whether inflammatory disease phenotypes are driven by increased NLRP3 expression, ASC-containing inflammasome assembly, caspase-1 cleavage, GSDMD cleavage, and extracellular release of IL-1β/IL-18 rather than by nonspecific cell injury alone. The pathway is strongly linked to inflammation-related disease phenotypes because monosodium urate crystals activate NALP3/NLRP3 inflammasome signaling in gout-like crystal inflammation, cholesterol crystals activate NLRP3 inflammasomes in atherogenesis models, and DSS-induced intestinal inflammation has been reported to involve NLRP3 inflammasome activity. However, experimental colitis studies also show context-dependent protective effects of NLRP3 inflammasome co
MTT Cell Proliferation Assay
The MTT assay is a colorimetric endpoint assay for estimating viable cell number, cell growth, cytotoxicity, or cell activation in cultured mammalian cells. Living cells reduce the yellow tetrazolium salt MTT into purple/blue formazan, while dead cells do not generate the same signal; the resulting color can be quantified with a multiwell spectrophotometer. MTT reduction is commonly interpreted as a readout of metabolic activity that often correlates with viable cell number, but it should not be treated as a direct cell-counting method unless the assay is optimized for the cell type and experimental condition. Studies show that MTT reduction can involve mitochondrial and non-mitochondrial reducing systems, and formazan may accumulate in intracellular lipid droplets rather than simply marking mitochondria.
Protocol For Protein Expression And Purification
Recombinant protein expression in Escherichia coli followed by purification of a His-tagged soluble protein by immobilized metal affinity chromatography (IMAC), with optional MBP fusion and TEV tag removal when the construct includes these elements. The biological readout is production of the encoded target protein, detected as an inducible band at the expected molecular mass by SDS-PAGE and quantified by total protein assay or chromatographic absorbance; the purification readout is enrichment of the target protein in elution fractions after selective binding of polyhistidine residues to immobilized Ni2+/metal-chelate resin and elution by imidazole-containing buffer. Expression is driven by an inducible bacterial expression system, commonly T7/lac-based, in which IPTG or lactose/auto-induction activates transcription and translation of the cloned gene; lower induction temperature, lower inducer concentration, induction timing, and solubility-enhancing fusion tags can influence the frac
Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.

순도&문서

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (276 KB)
SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Xu Z, et al. Dehydrocorydaline inhibits breast cancer cells proliferation by inducing apoptosis in MCF-7 cells. Am J Chin Med. 2012;40(1):177-85. [Content Brief]

[2]. Yoo M, et al. Dehydrocorydaline promotes myogenic differentiation via p38 MAPK activation. Mol Med Rep. 2016 Oct;14(4):3029-36. [Content Brief]

[3]. Nonaka M, et al. Screening of a library of traditional Chinese medicines to identify anti-malarial compounds and extracts. Malar J. 2018 Jun 25;17(1):244. [Content Brief]

[4]. Yin ZY, et al. Antinociceptive effects of dehydrocorydaline in mouse models of inflammatory pain involve the opioid receptor and inflammatory cytokines. Sci Rep. 2016 Jun 7;6:27129. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.7290 mL	13.6452 mL	27.2903 mL	68.2259 mL
	5 mM	0.5458 mL	2.7290 mL	5.4581 mL	13.6452 mL
	10 mM	0.2729 mL	1.3645 mL	2.7290 mL	6.8226 mL
	15 mM	0.1819 mL	0.9097 mL	1.8194 mL	4.5484 mL
	20 mM	0.1365 mL	0.6823 mL	1.3645 mL	3.4113 mL
	25 mM	0.1092 mL	0.5458 mL	1.0916 mL	2.7290 mL
	30 mM	0.0910 mL	0.4548 mL	0.9097 mL	2.2742 mL
	40 mM	0.0682 mL	0.3411 mL	0.6823 mL	1.7056 mL
	50 mM	0.0546 mL	0.2729 mL	0.5458 mL	1.3645 mL
	60 mM	0.0455 mL	0.2274 mL	0.4548 mL	1.1371 mL

Bioz

Keywords

Dehydrocorydaline

47 Cited Publications

100 mg 견적 받기

Subscribe to the

MedChemExpress

Newsletter

상품명:
Cat. No.:
수량:
MCE Japan Authorized Agent:

상품명:
CAS No.:
Species:
Purity:
수량:

Dehydrocorydaline

Publications Citing Use of MedChemExpress (MCE) Dehydrocorydaline

All Bcl-2 Family Isoforms

All Caspase Isoforms

All PARP Isoforms

All p38 MAPK Isoforms

All Parasite Isoforms