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Cat. No.: HY-163435

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Anticancer agent 201

Anticancer agent 201 (Compound 2f) has IC₅₀ values in the low micromolar range for multiple tumor cell lines. Anticancer agent 201 is highly cytotoxic to CCRF-CEM cells in vitro, inducing apotosis by activating caspase-3 in the intrinsic mitochondrial pathway and lysis of PARP, as well as reducing the expression of Bcl-2 and Bcl-XL proteins. Anticancer agent 201 can be used in cancer research.

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화학식: C₃₄H₄₉F₃O₄
분자량:578.75

보관:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (277 KB)

Anticancer agent 201 Chemical Structure

Amount: USD 0.00

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- 제품 설명
- IC₅₀ & Target
- In Vitro
Chemical Information
순도&문서
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

제품 설명

IC₅₀ & Target

Caspase-3

Bcl-2

Bcl-xL

All Caspase Isoforms :

All PARP Isoforms :

All Bcl-2 Family Isoforms :

In Vitro

Anticancer agent 201 (Compound 2f) (3.5 μM, 17.5 μM; 24h) significantly induces cytotoxic reactions, increases the number of apoptotic cells, and leads to a dose-dependent reduction of mitochondrial membrane potential in CCRF-CEM cells^[1].
Anticancer agent 201 (3.5 μM, 17.5 μM; 24h) blocks or slows down the G0/G1 phase cell cycle in CCRF-CEM cells, reduces the proportion of S-phase cells, and inhibits RNA synthesis^[1].

Cytotoxic activities of Anticancer agent 201 against eight tumor (including multidrug resistant variants) and two normal fibroblast cell lines^[1]

Cell lines	CCRF-CEM	CEM-DNR	K562	K562-TAX	A549	HCT116	HCT116p53^-/-	U20S	BJ	MRC-5
IC₅₀ (μM)	3.5	4.9	23	17	11	10	18	19	＞50	27

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Apoptosis Analysis^[1]

Cell Line:

CCRF-CEM cancer cell line
Concentration:

3.5 μM, 17.5 μM
Incubation Time:

24h
Result:

Induced the strongest cytotoxic reaction and the number of apoptotic cells increased significantly.
Resulted in a significant decrease in mitochondrial membrane potential of CCRF-CEM cells in a dose-dependent manner.

Cell Cycle Analysis^[1]

Cell Line:

CCRF-CEM cancer cell line
Concentration:

3.5 μM, 17.5 μM
Incubation Time:

24h
Result:

Caused the cell cycle to be blocked or slowed down in the G0/G1 phase, while the proportion of S phase cells decreases.
At 17.5 μM, the mitosis rate of the cells decreased.
At 3.5 μM increased the proportion of BRDU-positive cells.
At 17.5μM, the proportion of BRDU-positive cells decreased.
At 17.5μM, RNA synthesis almost completely stopped.

Western Blot Analysis^[1]

Cell Line:

CCRF-CEM cancer cell line
Concentration:

3.5 μM, 17.5 μM
Incubation Time:

24h
Result:

Leaded to decreased expression of both Bcl-2 and Bcl-XL.
At 17.5μM, caused the activation of caspase-3 and the cleavage of PARP.

Chemical Information

분자량 578.75
화학식 C₃₄H₄₉F₃O₄
SMILES

O=C1C=C(C2C[C@]3([C@@](C(C)(C2O1)C)([H])CC[C@@]4([C@]3([H])CC[C@]5([H])[C@@]6([H])[C@@H](CC[C@@]6(CC[C@]54C)C(O)=O)C(C)C)C)C)C(F)(F)F
선적

Room temperature in continental US; may vary elsewhere.
보관

Please store the product under the recommended conditions in the Certificate of Analysis.

순도&문서

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (277 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Kazakova A, et al. Novel triterpenoid pyrones, phthalimides and phthalates are selectively cytotoxic in CCRF-CEM cancer cells - Synthesis, potency, and mitochondrial mechanism of action. Eur J Med Chem. 2024;269:116336. [Content Brief]

Calculators

몰농도 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

농도 희석 계산기

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Bioz

Keywords

MOQ

Minimum order quantity

100 mg

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Amount: USD 0.00

Biological Activity
- 제품 설명
- IC₅₀ & Target
- In Vitro
Chemical Information
순도&문서
References

상품명:
Cat. No.:
수량:
MCE Japan Authorized Agent:

상품명:
CAS No.:
Species:
Purity:
수량:

Anticancer agent 201

All Caspase Isoforms

All PARP Isoforms

All Bcl-2 Family Isoforms