Polyporenic acid C
Based on 1 Customer Validation
Polyporenic acid C is a lanostane-type triterpenoid. Polyporenic acid C can be isolated from Poria cocos. Polyporenic acid C causes the cleavage of caspase-8 and caspase-3, as well as the cleavage of PARP. Polyporenic acid C reduces the phosphorylation level of Akt (Ser473), increases the phosphorylation of PTEN and p53 (Ser15), and activates JNK. Polyporenic acid C induces Apoptosis. Polyporenic acid C shows anticancer activity against non-small cell lung cancer.
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- Purity : 98.81%
- CAS No.: 465-18-9
- 화학식: C31H46O4
- 분자량:482.69
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보관:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Biological Activity
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Chemical Information
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용액&용해도
- Protocol
- 순도&문서
- References
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Help & FAQs
All Caspase Isoforms
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Biological Activity
제품 설명
IC50 & Target
[1]|
Caspase-8 |
Caspase-3 |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A549 | IC50 |
19.8 μM
Compound: 93
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Cytotoxicity against human A549 cells
Cytotoxicity against human A549 cells
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[PMID: 23092389] |
| BMDM | IC50 |
4.9 μM
Compound: 3
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Inhibition of RANKL-induced C57BL/6 mouse BMDM differentiation into osteoclasts after 4 days in presence of M-CSF by TRAP assay
Inhibition of RANKL-induced C57BL/6 mouse BMDM differentiation into osteoclasts after 4 days in presence of M-CSF by TRAP assay
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[PMID: 27266877] |
| CCRF-CEM | IC50 |
25.5 μM
Compound: 8
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Antiproliferative activity against human CCRF-CEM cells after 72 hrs by MTT assay
Antiproliferative activity against human CCRF-CEM cells after 72 hrs by MTT assay
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[PMID: 27808511] |
| HL-60 | IC50 |
31.8 μM
Compound: 8
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Antiproliferative activity against human HL60 cells after 72 hrs by MTT assay
Antiproliferative activity against human HL60 cells after 72 hrs by MTT assay
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[PMID: 27808511] |
| K562 | IC50 |
26.3 μM
Compound: 8
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Antiproliferative activity against human K562 cells after 72 hrs by MTT assay
Antiproliferative activity against human K562 cells after 72 hrs by MTT assay
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[PMID: 27808511] |
| MOLT-4 | IC50 |
27.6 μM
Compound: 8
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Antiproliferative activity against human MOLT4 cells after 72 hrs by MTT assay
Antiproliferative activity against human MOLT4 cells after 72 hrs by MTT assay
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[PMID: 27808511] |
In Vitro
Polyporenic acid C (0-200 μM; 24-72 h) inhibits the proliferation of human non-small cell lung cancer A549 cells in a dose- and time-dependent manner[1].
Polyporenic acid C (60-100 μM; 24-48 h) increases the apoptosis rate of human non-small cell lung cancer A549 cells in a dose- and time-dependent manner[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Human non-small cell lung cancer A549 cells
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Concentration:2 μM, 6 μM, 20 μM, 60 μM, 200 μM
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Incubation Time:24 h, 48 h, 72 h
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Result:Significantly inhibited cell viability (84% of the control group remained viable) (72 h, 6 μM).
Reduced the relative cell viability by more than 50% (72 h, 60 μM).
Inhibited cancer cell growth by more than 90% (72 h, 200 μM).
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Cell Line:Human non-small cell lung cancer A549 cells
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Concentration:60 μM
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Incubation Time:24 h (for caspase, Akt, PTEN, p53 detection); 3 h, 6 h, 24 h (for JNK detection)
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Result:Led to the cleavage of caspase-8 and caspase-3, and the cleavage of PARP (a substrate of caspase-3), but did not induce the cleavage of caspase-9.
Reduced the phosphorylation level of Akt (Ser473), increased the phosphorylation of PTEN and p53 (Ser15), and activated JNK.
Chemical Information
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CAS No. 465-18-9
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Appearance Solid
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분자량 482.69
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화학식 C31H46O4
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Color White to off-white
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SMILES
C[C@]12[C@@]([C@]([C@H](C(O)=O)CCC(C(C)C)=C)([H])[C@H](O)C1)(CC=C3C2=CC[C@](C4(C)C)([H])[C@@]3(CCC4=O)C)C
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Structure Classification
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Initial Source
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선적
Room temperature in continental US; may vary elsewhere.
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보관
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
용액&용해도
In Vitro:
DMSO : 16.67 mg/mL (34.54 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 50% PEG300 50% Saline
Solubility: 5 mg/mL (10.36 mM); Suspended solution; Need ultrasonic
순도&문서
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Data Sheet (275 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.0717 mL | 10.3586 mL | 20.7172 mL | 51.7931 mL |
| 5 mM | 0.4143 mL | 2.0717 mL | 4.1434 mL | 10.3586 mL | |
| 10 mM | 0.2072 mL | 1.0359 mL | 2.0717 mL | 5.1793 mL | |
| 15 mM | 0.1381 mL | 0.6906 mL | 1.3811 mL | 3.4529 mL | |
| 20 mM | 0.1036 mL | 0.5179 mL | 1.0359 mL | 2.5897 mL | |
| 25 mM | 0.0829 mL | 0.4143 mL | 0.8287 mL | 2.0717 mL | |
| 30 mM | 0.0691 mL | 0.3453 mL | 0.6906 mL | 1.7264 mL |
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