ODN 2007
Based on 1 Customer Validation
ODN 2007 is an immunostimulatory CpG oligodeoxynucleotide. ODN 2007 activates TLR21 (the chicken homolog of TLR9), triggering innate signaling. ODN 2007 induces CpG motif-specific, dose-dependent 2'5'-A synthetase. ODN 2007 stimulates lymph node IFN-α/IFN-γ secretion. ODN 2007 binds Hsp90α, activates ERK2/AKT, and induces Th1 cytokine/chemokine mRNA (IL-6, MIP-3α) and NO. ODN 2007 can be used in research on Escherichia coli infection.
For research use only. We do not sell to patients.
- Purity : 99.69%
- CAS No.: 455348-63-7
- Molecular Weight:6852.50
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Storage:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
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TLR21 |
HSP90α |
ERK2 |
IL-6 |
In Vitro
ODN 2007 (200 ng/mL; 16 h) induces chBMDC maturation, whereas ODN 2007-loaded CpG-NP treated at 200 ng/mL for 16 h produces a higher proportion of DC-like cells and higher CD40/CD80/CCR7 expression[1].
ODN 2007 (200 fmole; 20 min) directly binds to HD11 cytoplasmic and membrane proteins, with a stronger complex formed in the cytoplasmic F1 fraction[4].
ODN 2007 (10 µg/mL; 0-12 h) induces ERK2 phosphorylation starting at 30 min and AKT phosphorylation starting at 3 h in HD11 cells, and Hsp90, ERK1/2, and PI3K inhibitors reduce these induced phosphorylations[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HD11 avian macrophage cells
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Concentration:10 µg/mL (ODN 2007 sodium); 2 µM/mL (geldanamycin); 50 µM/mL (PD98059); 10, 50, 100 µM/mL (LY294002)
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Incubation Time:0, 1/2, 1, 3, 6, 12 h (stimulation); 60 min (inhibitor pre-incubation)
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Result:No significant difference was observed in Hsp90α or Hsp90β expression in stimulated or control HD11 cells.
A significant increase in phosphorylated ERK2 was detected in HD11 cells at 1/2 h, 1 h, 6 h, and 12 h.
A significant increase in phosphorylated AKT was observed at 3 h, 6 h, and 12 h; AKT phosphorylation started as early as 3 h, whereas ERK2 phosphorylation increased as early as 30 min.
Pre-incubation with geldanamycin (2 µM/mL) reduced phosphorylated ERK2 and phosphorylated AKT compared to control.
Pre-incubation with PD98059 (50 µM/mL) reduced phosphorylated ERK2 and phosphorylated AKT.
Pre-incubation with LY294002 (50 µM/mL) reduced phosphorylated ERK2 and phosphorylated AKT.
In Vivo
ODN 2007 (4 μg; subcutaneous injection, interscapular back; primary immunization and two boosts; at 2, 5, and 9 weeks of age) loaded in CpG-NP-adjuvanted rRBD immunization increases anti-rRBD antibody titers and reduces IBV re-isolation in the lungs and kidneys of SPF chickens[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:specific pathogen-free (SPF) (age at prime 2 weeks, boosted at 5 and 9 weeks)[1]
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Dosage:4 μg per chicken
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Administration:s.c. at the back between wings; prime and two boosts; at 2, 5, and 9 weeks of age
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Result:After the first boost, anti-rRBD antibody titer barely differed from the PBS control.
After IBV challenge, virus re-isolation rates were lung 4/5 and kidney 4/5.
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Animal Model:specific pathogen-free (SPF) (age at prime 2 weeks, boosted at 5 and 9 weeks)[1]
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Dosage:4 μg CpG per chicken
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Administration:s.c. at the back between wings; prime and two boosts; at 2, 5, and 9 weeks of age
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Result:After the first boost, anti-rRBD antibody titer was 5711, nearly 4-fold higher than controls.
After IBV challenge, virus re-isolation rates were lung 1/5 and kidney 2/5.
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Animal Model:SuVolk (either sex; 3-4 months of age; average body weight 46.1 kg)[2]
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Dosage:100 µg/kg (30% emulsigen/PBS); 100 µg/kg (PBS)
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Administration:s.c.; repeated 6 days later
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Result:Serum 2′5′-A synthetase levels increased after both primary and secondary injections.
Peak levels were consistently observed 4 days after each injection.
Serum 2′5′-A synthetase levels were consistently higher in sheep receiving emulsigen formulation than in sheep receiving PBS formulation.
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Animal Model:SuVolk (either sex; age range 1 week to 4 months)[2]
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Dosage:100 µg/kg (30% emulsigen); 100 µg/kg (PBS)
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Administration:s.c.; single injection
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Result:At 48 h after injection, marked perivascular mononuclear cell infiltration was observed; formulation in emulsigen resulted in greater accumulation of mononuclear cells and eosinophils.
The majority of accumulating cells were CD172a+ myeloid cells and MHC class II+ cells; few T cells and few CD1+ cells were detected.
Injection sites stained intensely for IFN-α whether formulated in emulsigen or PBS.
Intense IFN-γ staining was observed only with emulsigen formulation, whereas PBS formulation showed faint IFN-γ staining.
Chemical Information
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CAS No. 455348-63-7
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Appearance Solid
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Molecular Weight 6852.50
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Color White to off-white
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SMILES
[DNA, d(P-thio)(T-C-G-T-C-G-T-T-G-T-C-G-T-T-T-T-G-T-C-G-T-T)]
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Sequence
DNA, d(P-thio)(T-C-G-T-C-G-T-T-G-T-C-G-T-T-T-T-G-T-C-G-T-T)
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
In Vitro:
H2O : 20 mg/mL (2.92 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (278 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2242 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 0.1459 mL | 0.7297 mL | 1.4593 mL | 3.6483 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
ODN 2007 Related Classifications
HY-150734 Related Classifications
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Keywords
- ODN 2007
- 455348-63-7
- ODN2007
- ODN-2007
- Toll-like Receptor (TLR)
- IFNAR
- ERK
- Akt
- Interleukin Related
- NO Synthase
- CCR
- HSP
- 2'5'-A synthetase
- AKT
- B lymphocyte proliferation
- CpG motif
- ERK2
- Escherichia coli
- Hsp90α
- IFN-α
- IFN-γ
- NF-κB
- RNase L
- TLR21
- TLR9
- Th1 cytokine
- chBMDC
- dendritic cell maturation
- innate signaling
- macrophage
- nitric oxide
- proinflammatory cytokine
- Inhibitor
- inhibitor
- inhibit