Cat. No.: HY-N6602

α-Solanine

3 Cited Publications

Customer Review

Based on 3 publication(s) in Google Scholar

α-solanine, a bioactive component and one of the major steroidal glycoalkaloids in Solanum nigrum, has been observed to inhibit growth and induce apoptosis in cancer cells.

For research use only. We do not sell to patients.

Purity: 99.89%
CAS No.: 20562-02-1
Formula: C₄₅H₇₃NO₁₅
Molecular Weight:868.06

Storage:

Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month

α-Solanine Chemical Structure

CAS No.: 20562-02-1

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
- In Vivo
Chemical Information
Publications (3)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

α-solanine, a bioactive component and one of the major steroidal glycoalkaloids in Solanum nigrum, has been observed to inhibit growth and induce apoptosis in cancer cells^[1].

All Akt Isoforms :

All mTOR Isoforms :

All MMP Isoforms :

All NO Synthase Isoforms :

All COX Isoforms :

All NF-κB Isoforms :

In Vitro

α-Solanine (10-50 µM, 24 h) inhibits the cell viability of JEG-3, inhibits the cell invasion and migration through block of MMP, inhibits the colony formation^[2].
α-Solanine (0.5-5 µM, 24 h) inhibits the expression of LPS (HY-D1056)-induced iNOS, COX-2 and pro-inflammatory cytokine like IL-6、TNF-α、IL-1β, inhibits the activation of NF-κB signaling pathway^[3].
α-Solanine (10 µM, 24 h) induces autophagy in cancer cell by increasing intracellular ROS levels, activating ER stress, and inhibiting the Akt/mTOR signaling pathway.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:

JEG-3
Concentration:

30-50 µM
Incubation Time:

24 h
Result:

Inhibited the cell viability.

Real Time qPCR^[2]

Cell Line:

JEG-3
Concentration:

30 µM
Incubation Time:

24 h
Result:

Inhibited the expression of MMP-2 and MMP-9.

Cell Autophagy Assay^[4]

Cell Line:

A549
Concentration:

10 µM
Incubation Time:

48 h
Result:

Induced autophagy.

In Vivo

α-Solanine (5 mg/kg/day, ip for 7 days) exhibits antitumor activity in mouse models^[2].
α-Solanine (2.5-10 mg/kg, po., single dose) exhibits anti-inflammtory efficacy in LPS-induced sepsis mouse model^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

JEG-3 xenograft mouse model^[2]
Dosage:

5 mg/kg
Administration:

ip for 7 days
Result:

Inhibited the tumor growth.

Animal Model:

LPS-induced mouse sepsis model^[3]
Dosage:

2.5-10 mg/kg
Administration:

po., single dose
Result:

Improved the mice survival rate.

Chemical Information

CAS No. 20562-02-1
Appearance Solid
Molecular Weight 868.06
Formula C₄₅H₇₃NO₁₅
Color White to light yellow
SMILES

C[C@@]12[C@]3([H])[C@](N4[C@@](CC[C@H](C)C4)([H])[C@H]3C)([H])C[C@@]1([H])[C@@]5([H])[C@]([C@@]6(C(C[C@@H](O[C@@]7([H])[C@@H]([C@H]([C@@H](O)[C@@H](CO)O7)O[C@]8([H])O[C@@H]([C@@H](O)[C@H](O)[C@H]8O)CO)O[C@@]9([H])[C@@H]([C@@H]([C@@H](O)[C@H](C)O9)O)O)CC6)=CC5)C)([H])CC2
Structure Classification
- Steroids
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (3)

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Journal Impact Factor

Cancer Gene Ther

Demethoxycurcumin suppresses HK2-mediated glycolysis by targeting PTEN/Akt signaling. [Abstract]

2025 Oct 2. PMID: 41039015
Pest Manag Sci

Identification and functional characterization of a beta-glucosidase gene involved in solanine degradation in the ladybird beetle Henosepilachna vigintioctopunctata. [Abstract]

2026 Jan 7. PMID: 41502025
SSRN

2025 Feb 26.

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (57.60 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.1520 mL

5.7600 mL

11.5199 mL

5 mM

0.2304 mL

1.1520 mL

2.3040 mL

10 mM

0.1152 mL

0.5760 mL

1.1520 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 1.25 mg/mL (1.44 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 1.25 mg/mL (1.44 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 1.25 mg/mL (1.44 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (283 KB)
SDS (392 KB)
- English - EN (392 KB)
- Français - FR (392 KB)
- Deutsch - DE (392 KB)
- Norwegian - NO (392 KB)
- Español - ES (392 KB)
- Swedish - SV (392 KB)
- Italian - IT (392 KB)
- Korean - KR (392 KB)
- Portuguese - PT (392 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Lei Wang , et al. Inhibitory Effect of α-Solanine on Esophageal Carcinoma in vitro. Exp Ther Med. 2016 Sep;12(3):1525-1530. [Content Brief]

[2]. Gu T, et al., α-Solanine Inhibits Proliferation, Invasion, and Migration, and Induces Apoptosis in Human Choriocarcinoma JEG-3 Cells In Vitro and In Vivo. Toxins (Basel). 2021 Mar 13;13(3):210. [Content Brief]

[3]. Shin JS, et al., α-Solanine Isolated From Solanum Tuberosum L. cv Jayoung Abrogates LPS-Induced Inflammatory Responses Via NF-κB Inactivation in RAW 264.7 Macrophages and Endotoxin-Induced Shock Model in Mice. J Cell Biochem. 2016 Oct;117(10):2327-39. [Content Brief]

[4]. Hasanain M, et al., α-Solanine induces ROS-mediated autophagy through activation of endoplasmic reticulum stress and inhibition of Akt/mTOR pathway. Cell Death Dis. 2015 Aug 27;6(8):e1860. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.1520 mL	5.7600 mL	11.5199 mL	28.7999 mL
	5 mM	0.2304 mL	1.1520 mL	2.3040 mL	5.7600 mL
	10 mM	0.1152 mL	0.5760 mL	1.1520 mL	2.8800 mL
	15 mM	0.0768 mL	0.3840 mL	0.7680 mL	1.9200 mL
	20 mM	0.0576 mL	0.2880 mL	0.5760 mL	1.4400 mL
	25 mM	0.0461 mL	0.2304 mL	0.4608 mL	1.1520 mL
	30 mM	0.0384 mL	0.1920 mL	0.3840 mL	0.9600 mL
	40 mM	0.0288 mL	0.1440 mL	0.2880 mL	0.7200 mL
	50 mM	0.0230 mL	0.1152 mL	0.2304 mL	0.5760 mL

Keywords

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α-Solanine

Apoptosis Compound Library

Immunology/Inflammation Compound Library

Kinase Inhibitor Library

Metabolism/Protease Compound Library

NF-κB Signaling Compound Library

PI3K/Akt/mTOR Compound Library

Stem Cell Signaling Compound Library

Natural Product Library

Anti-Cancer Compound Library

Autophagy Compound Library

Anti-Aging Compound Library

Antioxidant Compound Library

Differentiation Inducing Compound Library

Reprogramming Compound Library

Glycoside Compound Library

Oxygen Sensing Compound Library

Endoplasmic Reticulum Stress Compound Library

Medicine Food Homology Compound Library

Glycolysis Compound Library

Pyroptosis Compound Library

Cytoskeleton Compound Library

Orally Active Compound Library

Glutamine Metabolism Compound Library

Traditional Chinese Medicine Active Compound Library

Neuroprotective Compound Library

Anti-Breast Cancer Compound Library

Anti-Lung Cancer Compound Library

Anti-Pancreatic Cancer Compound Library

Anti-Blood Cancer Compound Library

Anti-Cancer Metabolism Compound Library

Anti-Obesity Compound Library

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Transcription Factor-Targeted Library

Glucose Metabolism Compound Library

Food-Sourced Compound Library

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Anti-Colorectal Cancer Compound Library

Anti-Cancer Natural Product Library

Antidepressant Compound Library

Anti-inflammatory Traditional Chinese Medicine Active Compound Library

Plant-Sourced Natural Product Library

Human Metabolite Library

Anti-Prostate Cancer Compound Library

Anti-Pulmonary Fibrosis Compound Library

Cancer Stem Cells Compound Library

Pain-Related Compound Library

Mitochondrial Protection Compound Library

Metabolic Enzyme Compound Library

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Cell Death Library

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MCE Bioactive Compound Library

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Miao Ethnicity Medicine Compound Library

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Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

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