Chaetoglobosin Vb
Chaetoglobosin Vb is a cytochalasin alkaloid with anti-inflammatory and antioxidant activities. Chaetoglobosin Vb attenuates LPS (HY-D1056)-induced TLR4 inflammatory signaling and IFN-β release by downregulating TRIF, MyD88, TLR4 mRNA and IRF7 expression. Chaetoglobosin Vb downregulates the expression and release of inflammatory mediators such as iNOS, COX-2, TNF-α and IL-6 by blocking MAPK/NF-κB pathway activation. Chaetoglobosin Vb reduces excessive ROS production and protects SOD enzyme activity against LPS-induced oxidative stress. Chaetoglobosin Vb inhibits the growth of certain bacteria and radish seedling roots. Chaetoglobosin Vb can be used in research related to colorectal cancer, bacterial infection, and radish growth.
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- CAS. Nr.: 1399690-75-5
- Formel: C32H36N2O5
- Molecular Weight:528.64
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Speicherung:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biologische Aktivität
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Chemical Information
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Biologische Aktivität
Beschreibung
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TLR4 |
ERK1 |
ERK2 |
COX-2 |
IL-6 |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
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| HCT-116 | IC50 |
18.4 μM
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Cytotoxicity against human HCT116 colorectal cancer cells assessed as reduction in cell viability incubated for 72 hrs by SRB colorimetric assay.
Cytotoxicity against human HCT116 colorectal cancer cells assessed as reduction in cell viability incubated for 72 hrs by SRB colorimetric assay.
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24708412 |
In Vitro
Chaetoglobosin Vb (25-200 μM; 24 h) shows no cytotoxicity to RAW264.7 cells[1].
Chaetoglobosin Vb (25-100 μM; pretreatment for 1 h) inhibits LPS-induced protein expression of iNOS and COX-2 in RAW264.7 cells and suppresses the activation of MAPK and NF-κB signaling pathways[1].
Chaetoglobosin Vb (25-100 μM; pretreatment for 1 h) reduces LPS (HY-D1056)-induced NO production in RAW264.7 cells[1].
Chaetoglobosin Vb (25-100 μM; pretreatment for 1 h) inhibits LPS-induced TNF-α and IL-6 secretion in RAW264.7 cells[1].
Chaetoglobosin Vb (100 μM; 1 h pretreatment) downregulates the mRNA expression of iNOS, COX-2, TNF-α, IL-6, IL-1β, TLR4, MyD88, TRIF, and IRF7 in LPS-stimulated RAW264.7 cells, while having no significant inhibitory effect on IRF3[1].
Chaetoglobosin Vb (25-100 μM) effectively inhibits LPS-induced excessive ROS production in RAW264.7 cells[1].
Chaetoglobosin Vb (25-100 μM) maintains SOD activity in LPS-stimulated RAW264.7 cells[1].
Chaetoglobosin Vb (16 h) exhibits weak antibacterial activity against Enterococcus faecalis ATCC 29212, ESBL-producing Escherichia coli ATCC 35218, and NDM-1-producing Klebsiella pneumoniae ATCC BAA2146 (MIC = 64 μg/mL for all), and shows no activity against MRSA ATCC 43300 and Pseudomonas aeruginosa ATCC 15542 (MIC >128 μg/mL)[3].
Chaetoglobosin Vb (72 h) exhibits weak cytotoxicity against HCT116 human colorectal cells with an IC50 of 18.4 μM[4].
Chaetoglobosin Vb (50-200 ppm; 4 days) inhibits radish seedling root growth in a concentration-dependent manner[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:RAW264.7
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Concentration:25, 50, 100, 200 μM
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Incubation Time:24 h
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Result:Exhibited cytotoxicity on RAW264.7 cells at 200 μM.
Showed no cytotoxic effects at 25, 50 or 100 μM.
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Cell Line:RAW264.7
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Concentration:25, 50, 100 μM
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Incubation Time:Pretreatment for 1 h
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Result:At 25 μM, seemed unable to significantly reduce LPS-induced TNF-α secretion, whereas at 50 or 100 μM were effective.
At 25, 50 or 100 μM, significantly suppressed the increase of IL-6 content.
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Cell Line:RAW264.7
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Concentration:100 μM
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Incubation Time:Pretreatment for 1 h
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Result:Markedly down-regulated the increased gene expression of iNOS and reduced the mRNA expression level of COX-2 in LPS-stimulated RAW264.7 cells.
Facilitated a considerable reduction of the increased mRNA expression of TNF-α, IL-6, and IL-1β.
Inhibited the expression of TLR4 mRNA, and reduced its downstream signaling pathways MyD88 and TRIF.
The level of TRIF downstream component IRF3 seemed to decrease (although such a decrease was insignificant), but the level of IRF7 decreased significantly.
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Cell Line:RAW264.7
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Concentration:25, 50, 100 μM
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Incubation Time:Pretreatment for 1 h
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Result:At 100 μM, markedly down-regulated the increased protein expression of iNOS in LPS-stimulated RAW264.7 cells.
At 25, 50 or 100 μM, significantly reduced COX-2 protein expression.
Pre-treatment dose-dependently lowered the extent of P-p38, P-ERK1/2 and P-JNK (25 μM was almost ineffective, 50 μM effective, and 100 μM extremely effective).
At 25, 50 or 100 μM, displayed a significant suppression on the phosphorylation of IκB and nuclear translocation of NF-κB p65.
The impact at 25 or 50 μM were almost the same, with the concentration of 100 μM being the most effective.
Chemical Information
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CAS. Nr. 1399690-75-5
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Molecular Weight 528.64
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Formel C32H36N2O5
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SMILES
O=C1N[C@@H](CC2=CNC3=C2C=CC=C3)[C@]4([H])C(C)=C(C)[C@@H](O)[C@@](/C=C/C[C@H](C)[C@]5([H])[C@@](C(C(O)=C5C)=O)([H])C6)([H])[C@]14C6=O
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Structure Classification
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Initial Source
Chaetomium globosum
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Please store the product under the recommended conditions in the Certificate of Analysis.
Reinheit & Dokumentation
Verweise
[1]. Zhang H, et al. Anti-inflammatory and antioxidant effects of Chaetoglobosin V in LPS-induced RAW264.7 cells: Achieved via the MAPK and NF-κB signaling pathways. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. 2021 Jan;147:111915. [Content Brief]
Calculators
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Chaetoglobosin Vb Related Classifications
HY-N9541 Related Classifications
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