製品番号: HY-B0877

ハルシノニド

(別名: Halcinonide; SQ-18566)

1 Cited Publications

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Based on 1 publication(s) in Google Scholar

Halcinonide (SQ-18566) is an orally active Smoothened (Smo) agonist. Halcinonide activates the Hedgehog signaling pathway by binding to Smo and promoting its internalization and expression, thereby activating Gli transcription factors. Halcinonide not only stimulates cell proliferation, increases the expression of cyclin D2/CDK6 and inhibits the degradation of caspase-3, but also suppresses Bcl-2/Bax-mediated apoptosis, oxidative stress and inflammatory responses. Halcinonide activates RxRγ to upregulate the expression of myelin genes, thereby reducing cerebral infarction and improving behavioral deficits. Halcinonide has been used in studies related to multiple sclerosis and ischemic stroke.

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純度: 98.29%
CAS 番号: 3093-35-4
分子式: C₂₄H₃₂ClFO₅
分子量:454.96

保管条件:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

Halcinonide 構造式

CAS 番号: 3093-35-4

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Amount: USD 0.00

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生物活性
- 製品説明
- IC₅₀ & Target
- 体外実験
- 体内実験
化学情報
Publications (1)
溶剤 & 溶解度
- 体外
純度とドキュメンテーション
参考文献
Help & FAQs
関連分類
Bioz

生物活性

製品説明

IC₅₀ & Target

Cdk6/cyclin D2

Caspase 3

Caspase アイソフォーム固有の製品をすべて表示 :

RAR/RXR アイソフォーム固有の製品をすべて表示 :

CDK アイソフォーム固有の製品をすべて表示 :

体外実験

Halcinonide (1-25 μM; 48 h) acts as a Smoothened agonist to up-regulate MBP, PLP, CNP, MOG, RxRγ, and Gli1 gene expression in Oli-neuM mouse oligodendrocyte cells, with 10 μM treatment for 48h driving significant myelin gene induction that is dependent on both Smoothened and RxRγ activation^[1].
Halcinonide (0-10 μM; 2 h) acts as a Smo agonist in U2OS cells stably expressing Smo-633 and βarr2-GFP, inducing βarr2-GFP aggregation with an EC₅₀ of 1,100 nM and an efficacy of 0.99^[2].
Halcinonide (0-10 μM; 30 h) activates downstream Hedgehog signaling in Shh-LIGHT2 cells, inducing Gli-luciferase activity with an EC₅₀ of 1.8 nM and an efficacy of 0.74, and synergizes with Shh to enhance this activity^[2].
Halcinonide (25 μM; 64 h) up-regulates cyclin D2 expression and inhibits caspase-3 degradation in primary mouse cerebellar granule cell precursors^[2].
Halcinonide (10-100 nM; 24 h) protects PC12 cells from H₂O₂-induced oxidative stress, improving cell viability and reducing intracellular ROS generation^[3].
Halcinonide (10-100 nM; 24 h) modulates the Shh signaling pathway and Bcl-2/Bax-mediated apoptosis in PC12 cells treated with H₂O₂, upregulating SMO and Bcl-2 while downregulating Sufu, Bax, and Caspase-3^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[3]

Cell Line:

PC12 cells (under OGD/R stress)
Concentration:

10 nM, 100 nM (24 h pre-incubation)
Incubation Time:

24 h (pre-incubation); 6 h (OGD); 24 h (reperfusion)
Result:

Reversed OGD/R-induced decreases in SMO and Bcl-2 protein levels.
Reversed OGD/R-induced increases in Sufu, Bax, and Caspase-3 protein levels.

体内実験

Halcinonide (20 mg/kg; p.o.; daily; 7 days) ameliorates ischemic stroke injury in MCAO rats by reducing cerebral infarction, improving neurological function, suppressing apoptosis, restoring redox homeostasis, and reducing inflammation via activation of the SMO/Shh pathway^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Sprague-Dawley rat with Ischemic stroke (male, 2-3 months old, 180-220 g, SPF grade, middle cerebral artery occlusion/reperfusion model)^[3]
Dosage:

20 mg/kg
Administration:

p.o.; daily; 7 days
Result:

Reduced neurological deficit scores relative to untreated MCAO rats.
Reduced cerebral infarction volume relative to untreated MCAO rats.
Improved abnormal brain histological features observed via H&E staining.
Reduced the number of TUNEL-positive apoptotic cells in brain tissue.
Upregulated SMO and Bcl-2 protein expression in brain tissue.
Downregulated Sufu, Bax, and Caspase-3 protein expression in brain tissue.
Increased serum LDH activity.
Reduced serum lactic acid levels.
Restored serum MPO, MDA, SOD, GSH, and GSSG levels toward normal.
Reduced serum TNF-α and IL-6 levels.

化学情報

CAS 番号 3093-35-4
性状 Solid
分子量 454.96
分子式 C₂₄H₃₂ClFO₅
Color Light yellow to yellow
SMILES

C[C@@]12[C@@]3(C(CCl)=O)[C@@](OC(C)(O3)C)([H])C[C@@]1([H])[C@]4([H])CCC5=CC(CC[C@]5(C)[C@@]4(F)[C@@H](O)C2)=O
別名

Halcinonide; SQ-18566
輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (1)

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Nat Commun

Coronavirus envelope protein activates TMED10-mediated unconventional secretion of inflammatory factors. [Abstract]

2024 Oct 8;15(1):8708. PMID: 39379362

溶剤 & 溶解度

体外:

DMSO : 100 mg/mL (219.80 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.1980 mL

10.9900 mL

21.9800 mL

5 mM

0.4396 mL

2.1980 mL

4.3960 mL

10 mM

0.2198 mL

1.0990 mL

2.1980 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

モル濃度計算機

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

濃度濃度

体積体積

分子量分子量

希釈計算機

濃度 _(開始) × 体積 _(開始) = 濃度 _(終了) × 体積 _(終了)

濃度 _(開始) 濃度 _(開始)

体積 _(開始) 体積 _(開始)

濃度 _(終了) 濃度 _(終了)

体積 _(終了) 体積 _(終了)

純度とドキュメンテーション

バッチを選択:

純度: 99.98%

Assay: 99.98%

ee.: 99.98%

データシート (284 KB)
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- Deutsch - DE (418 KB)
- Norwegian - NO (418 KB)
- Español - ES (418 KB)
- Swedish - SV (418 KB)
- Italian - IT (418 KB)
- Korean - KR (418 KB)
- Portuguese - PT (418 KB)
COA (2659 KB)
元素分析報告書 (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
取扱説明書 (2659 KB)

参考文献

[1]. Porcu G, et al. Clobetasol and Halcinonide Act as Smoothened Agonists to Promote Myelin Gene Expression and RxRγ Receptor Activation. PLoS One. 2015;10(12):e0144550. Published 2015 Dec 10. [Content Brief]

[2]. Wang J, et al. Identification of select glucocorticoids as Smoothened agonists: potential utility for regenerative medicine. Proc Natl Acad Sci U S A. 2010;107(20):9323-9328. [Content Brief]

[3]. Liu J, et al. Halcinonide activates smoothened to ameliorate ischemic stroke injury. Life Sci. 2025;361:123324. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.1980 mL	10.9900 mL	21.9800 mL	54.9499 mL
	5 mM	0.4396 mL	2.1980 mL	4.3960 mL	10.9900 mL
	10 mM	0.2198 mL	1.0990 mL	2.1980 mL	5.4950 mL
	15 mM	0.1465 mL	0.7327 mL	1.4653 mL	3.6633 mL
	20 mM	0.1099 mL	0.5495 mL	1.0990 mL	2.7475 mL
	25 mM	0.0879 mL	0.4396 mL	0.8792 mL	2.1980 mL
	30 mM	0.0733 mL	0.3663 mL	0.7327 mL	1.8317 mL
	40 mM	0.0549 mL	0.2747 mL	0.5495 mL	1.3737 mL
	50 mM	0.0440 mL	0.2198 mL	0.4396 mL	1.0990 mL
	60 mM	0.0366 mL	0.1832 mL	0.3663 mL	0.9158 mL
	80 mM	0.0275 mL	0.1374 mL	0.2747 mL	0.6869 mL
	100 mM	0.0220 mL	0.1099 mL	0.2198 mL	0.5495 mL

Halcinonide

1 Cited Publications

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MedChemExpress（MCE）の使用を引用している文献 Halcinonide

Caspase アイソフォーム固有の製品をすべて表示

RAR/RXR アイソフォーム固有の製品をすべて表示

CDK アイソフォーム固有の製品をすべて表示

生物活性

化学情報

Publications (1)

溶剤 & 溶解度

純度とドキュメンテーション

参考文献

Help & FAQs

Complete Stock Solution Preparation Table

Halcinonide 関連分類

HY-B0877 関連分類