Chaetoglobosin Vb
Chaetoglobosin Vb is a cytochalasin alkaloid with anti-inflammatory and antioxidant activities. Chaetoglobosin Vb attenuates LPS (HY-D1056)-induced TLR4 inflammatory signaling and IFN-β release by downregulating TRIF, MyD88, TLR4 mRNA and IRF7 expression. Chaetoglobosin Vb downregulates the expression and release of inflammatory mediators such as iNOS, COX-2, TNF-α and IL-6 by blocking MAPK/NF-κB pathway activation. Chaetoglobosin Vb reduces excessive ROS production and protects SOD enzyme activity against LPS-induced oxidative stress. Chaetoglobosin Vb inhibits the growth of certain bacteria and radish seedling roots. Chaetoglobosin Vb can be used in research related to colorectal cancer, bacterial infection, and radish growth.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- CAS 番号: 1399690-75-5
- 分子式: C32H36N2O5
- 分子量:528.64
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保管条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
- プロトコル
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
Endogenous Metabolite アイソフォーム固有の製品をすべて表示
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生物活性
製品説明
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TLR4 |
ERK1 |
ERK2 |
COX-2 |
IL-6 |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HCT-116 | IC50 |
18.4 μM
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Cytotoxicity against human HCT116 colorectal cancer cells assessed as reduction in cell viability incubated for 72 hrs by SRB colorimetric assay.
Cytotoxicity against human HCT116 colorectal cancer cells assessed as reduction in cell viability incubated for 72 hrs by SRB colorimetric assay.
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24708412 |
体外実験
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:RAW264.7
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Concentration:25, 50, 100, 200 μM
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Incubation Time:24 h
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Result:Exhibited cytotoxicity on RAW264.7 cells at 200 μM.
Showed no cytotoxic effects at 25, 50 or 100 μM.
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Cell Line:RAW264.7
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Concentration:25, 50, 100 μM
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Incubation Time:Pretreatment for 1 h
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Result:At 25 μM, seemed unable to significantly reduce LPS-induced TNF-α secretion, whereas at 50 or 100 μM were effective.
At 25, 50 or 100 μM, significantly suppressed the increase of IL-6 content.
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Cell Line:RAW264.7
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Concentration:100 μM
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Incubation Time:Pretreatment for 1 h
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Result:Markedly down-regulated the increased gene expression of iNOS and reduced the mRNA expression level of COX-2 in LPS-stimulated RAW264.7 cells.
Facilitated a considerable reduction of the increased mRNA expression of TNF-α, IL-6, and IL-1β.
Inhibited the expression of TLR4 mRNA, and reduced its downstream signaling pathways MyD88 and TRIF.
The level of TRIF downstream component IRF3 seemed to decrease (although such a decrease was insignificant), but the level of IRF7 decreased significantly.
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Cell Line:RAW264.7
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Concentration:25, 50, 100 μM
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Incubation Time:Pretreatment for 1 h
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Result:At 100 μM, markedly down-regulated the increased protein expression of iNOS in LPS-stimulated RAW264.7 cells.
At 25, 50 or 100 μM, significantly reduced COX-2 protein expression.
Pre-treatment dose-dependently lowered the extent of P-p38, P-ERK1/2 and P-JNK (25 μM was almost ineffective, 50 μM effective, and 100 μM extremely effective).
At 25, 50 or 100 μM, displayed a significant suppression on the phosphorylation of IκB and nuclear translocation of NF-κB p65.
The impact at 25 or 50 μM were almost the same, with the concentration of 100 μM being the most effective.
化学情報
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CAS 番号 1399690-75-5
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分子量 528.64
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分子式 C32H36N2O5
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SMILES
O=C1N[C@@H](CC2=CNC3=C2C=CC=C3)[C@]4([H])C(C)=C(C)[C@@H](O)[C@@](/C=C/C[C@H](C)[C@]5([H])[C@@](C(C(O)=C5C)=O)([H])C6)([H])[C@]14C6=O
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Structure Classification
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Initial Source
Chaetomium globosum
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Please store the product under the recommended conditions in the Certificate of Analysis.
純度とドキュメンテーション
参考文献
[1]. Zhang H, et al. Anti-inflammatory and antioxidant effects of Chaetoglobosin V in LPS-induced RAW264.7 cells: Achieved via the MAPK and NF-κB signaling pathways. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. 2021 Jan;147:111915. [Content Brief]
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
Chaetoglobosin Vb 関連分類
HY-N9541 関連分類
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