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製品番号: HY-189656

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PY-Alo

PY-Alo is a DNA intercalator with a K_a of 3.46 × 10⁵ M⁻¹. PY-Alo induces DNA damage through backbone intercalation and non-covalent hydrogen bonding, downregulates the transcription of key genes in mitochondrial oxidative phosphorylation, and disrupts mitochondrial homeostasis, thereby synergistically inducing apoptosis (apoptosis) and autophagy (autophagy) in cells. PY-Alo exhibits cytotoxicity against both wild-type and Cisplatin (HY-17394)-resistant ovarian cancer cells. PY-Alo can be used for research on ovarian cancer.

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。

分子式: C₃₃H₄₃ClN₄
分子量:531.17

保管条件:

Please store the product under the recommended conditions in the Certificate of Analysis.

PY-Alo 構造式

価格（税別）

Amount: USD 0.00

* アイテムを追加する前、数量をご選択ください

Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

生物活性
- 製品説明
- IC₅₀ & Target
- Cellular Effect
- 体外実験
- 体内実験
化学情報
純度とドキュメンテーション
参考文献
Calculators
Help & FAQs
関連分類
Bioz

生物活性

製品説明

IC₅₀ & Target

^[1]

Caspase-9

Caspase 3

Bax

Bcl-2

MMP

MMP アイソフォーム固有の製品をすべて表示 :

Bcl-2 Family アイソフォーム固有の製品をすべて表示 :

Caspase アイソフォーム固有の製品をすべて表示 :

Cellular Effect

Cell Line	Type	Value	Description	References
SK-OV-3	IC₅₀	2.57 μM	Cytotoxicity against human ovarian cancer SKOV3 cells assessed as viability reduction by cell viability assay. Cytotoxicity against human ovarian cancer SKOV3 cells assessed as viability reduction by cell viability assay.	acs.jmedchem.6c00799
A2780	IC₅₀	2.34 μM	Cytotoxicity against human ovarian cancer A2780 cells assessed as viability reduction by cell viability assay. Cytotoxicity against human ovarian cancer A2780 cells assessed as viability reduction by cell viability assay.	acs.jmedchem.6c00799

体外実験

Anti-cancerOxidative StressMitochondrial FunctionMitochondrial Oxidative PhosphorylationApoptosisAutophagyOthers

Anti-cancer

PY-Alo (0.25-8 μM; 24 h) inhibits the viability of ovarian cancer SKOV3, A2780, and A2780/DDP cells with IC₅₀ values of 2.57, 2.34, and 2.89 μM, respectively, while showing lower toxicity to normal ovarian SKOV3 cells with an IC₅₀ of 7.21 μM^[1].
PY-Alo (3.0-5.0 μM; 30 min-24 h) accumulates in the nuclear DNA of A2780/DDP cells and induces γ-H2AX DNA damage^[1].
PY-Alo (1.0 μM; 24 h) exhibits activity in A2780/DDP cells by synergizing with Cisplatin (HY-17394) and reducing the viability of DDP-resistant cells^[1].

Oxidative Stress

Mitochondrial Function

Anti-cancer

Mitochondrial Oxidative Phosphorylation

Apoptosis

Autophagy

PY-Alo (3.0 μM; 24 h) inhibits cell migration and invasion in A2780 and A2780/DDP cells, disrupts mitochondrial function (increases intracellular Ca²⁺, ROS, and Mito-ROS while decreasing MMP) and redox homeostasis (decreases intracellular ATP and GSH levels), induces apoptosis and autophagy (increases the Bax/Bcl-2 ratio and the protein expression levels of p53, Cleaved Caspase-9/3, LC3B II, and p62), induces mitochondrial structural damage, and induces autolysosome formation^[1].

Anti-cancer

Mitochondrial Function

Mitochondrial Oxidative Phosphorylation

PY-Alo (3.0 μM; 24 h) downregulates the mRNA expression levels of mitochondrial respiratory chain complex core subunits in A2780/DDP cells^[1].

Others

PY-Alo (10.0 μM) selectively binds dsDNA with a binding constant of 3.46 × 10⁵ M⁻¹^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

IOSE-80, SKOV3, A2780, A2780/DDP
Concentration:

0.25, 0.5, 1, 2, 4, 8 μM
Incubation Time:

24 h
Result:

Inhibited cell viability in all four cell lines and exhibited significant cytotoxicity against cisplatin-resistant A2780/DDP cells with an IC₅₀ of 2.89 μM.

Immunofluorescence^[1]

Cell Line:

A2780, A2780/DDP
Concentration:

3.0 μM
Incubation Time:

24 h
Result:

Induced significant DNA damage, evidenced by strong green fluorescence of γ-H2AX in both A2780 and A2780/DDP cells.

Western Blot Analysis^[1]

Cell Line:

A2780, A2780/DDP
Concentration:

3.0 μM
Incubation Time:

24 h
Result:

Elevated the Bax/Bcl-2 ratio and p53 levels, induced Cleaved Caspase-9/3 expression, and increased LC3B II and p62 levels, triggering a synergistic apoptosis-autophagy pathway.

Cell Migration Assay ^[1]

Cell Line:

A2780, A2780/DDP
Concentration:

3.0 μM
Incubation Time:

24 h
Result:

Inhibited cell migration in both A2780 and A2780/DDP cells.

Cell Invasion Assay^[1]

Cell Line:

A2780, A2780/DDP
Concentration:

3.0 μM
Incubation Time:

24 h
Result:

Inhibited cell invasion in both A2780 and A2780/DDP cells.

Real Time qPCR^[1]

Cell Line:

A2780/DDP
Concentration:

3.0 μM
Incubation Time:

24 h
Result:

Significantly downregulated the mRNA expression of multiple subunits of the NADH dehydrogenase (MT-ND), cytochrome c (MT-CYB), cytochrome c oxidase (MT-CO), and ATP synthase (MT-ATP).

体内実験

Anti-cancerApoptosisAutophagy

Anti-cancer

Apoptosis

Autophagy

PY-Alo (10 mg/kg; i.v.; once daily; 15 administrations) effectively inhibits tumor growth and induces DNA damage, apoptosis, and autophagy markers in A2780/DDP cisplatin-resistant ovarian cancer xenografts^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

BALB/c nude (female) were injected with 5×10⁶ A2780 or A2780/DDP cells into the unilateral axilla^[1]
Dosage:

10 mg/kg
Administration:

i.v.; once daily; 15 days
Result:

Effectively inhibited tumor growth (decreased tumor volume and tumor weight) in both A2780 and cisplatin-resistant A2780/DDP xenograft models.
Demonstrated acceptable biosafety, showing minimal influence on mouse body weight and minimal damage to major organs (heart, liver, spleen, lungs, and kidneys).
Induced DNA damage (γ-H2AX), apoptosis (Ki67 reduction), and autophagy (LC3B II and p62 increase) in the tumor tissues in vivo.

Related Protocols :

化学情報

分子量 531.17
分子式 C₃₃H₄₃ClN₄
SMILES

CN(C1=CC(CC2=C/C3=[N+](C)\C)=C(C(N4[C@]5([C@H]6CN7CCCC[C@@]7([C@H](C6)C=C5CCC4)[H])[H])=C2C=C3)C=C1)C.[Cl-]
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件

Please store the product under the recommended conditions in the Certificate of Analysis.

純度とドキュメンテーション

取扱説明書 (2659 KB)

参考文献

[1]. Wang LL, et al. A Non-Metallic DNA Intercalator from Pyronine-Aloperine Conjugation Reverses Cisplatin Resistance in Ovarian Cancer. J Med Chem. 2026;69:21220-21232.

Calculators

モル濃度計算機

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

濃度濃度

体積体積

分子量分子量

希釈計算機

濃度 _(開始) × 体積 _(開始) = 濃度 _(終了) × 体積 _(終了)

濃度 _(開始) 濃度 _(開始)

体積 _(開始) 体積 _(開始)

濃度 _(終了) 濃度 _(終了)

体積 _(終了) 体積 _(終了)

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Bioz

MOQ

Minimum order quantity

100 mg

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Other size

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Amount: USD 0.00

生物活性
- 製品説明
- IC₅₀ & Target
- 体外実験
- 体内実験
化学情報
純度とドキュメンテーション
参考文献

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PY-Alo

MMP アイソフォーム固有の製品をすべて表示

Bcl-2 Family アイソフォーム固有の製品をすべて表示

Caspase アイソフォーム固有の製品をすべて表示

生物活性

製品説明

IC₅₀ & Target

Cellular Effect

体外実験

体内実験

化学情報

純度とドキュメンテーション

参考文献

Calculators

Help & FAQs

Bioz

Keywords

最近チェックした製品

PY-Alo

MMP アイソフォーム固有の製品をすべて表示

Bcl-2 Family アイソフォーム固有の製品をすべて表示

Caspase アイソフォーム固有の製品をすべて表示

生物活性

製品説明

IC50 & Target

Cellular Effect

体外実験

体内実験

化学情報

純度とドキュメンテーション

参考文献

Calculators

Help & FAQs

PY-Alo 関連分類

HY-189656 関連分類

Bioz

Keywords

最近チェックした製品

IC₅₀ & Target