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Cat. No.: HY-130413

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Protectin D1

(Synonyms: Neuroprotectin D1; NPD1)

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Based on 1 Customer Validation

Protectin D1, a neuroprotectin D1 produced by neuronal cells, is a member of a newly discovered family of bioactive products derived from docosahexaenoic acid. Protectin D1 also serves as a specialized pro-resolving mediator, exhibiting effective in vivo pro-resolving activity in various human disease models. Additionally, Protectin D1 is an inhibitor of NALP3 inflammasomes and regulates the PI3K/AKT and HIF-1α signaling pathways. Protectin D1 exerts anti-inflammatory effects by reducing ROS levels, inhibiting the expression of NALP3, ASC, and Caspase-1, and consequently decreasing the release of pro-inflammatory cytokines IL-1β and IL-18. Furthermore, Protectin D1 enhances miRNA-210 expression, activates the PI3K/AKT signaling pathway, and exerts cardioprotective effects. Protectin D1 holds promise for research in cardiovascular diseases and inflammatory disorders.

For research use only. We do not sell to patients.

Purity: 99.0%
CAS No.: 660430-03-5
Formula: C₂₂H₃₂O₄
Molecular Weight:360.49

Storage:

Solution, -20°C, 2 years

Protectin D1 Chemical Structure

CAS No.: 660430-03-5

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

Human Endogenous Metabolite

IL-1β

All Endogenous Metabolite Isoforms :

All PI3K Isoforms :

All Akt Isoforms :

All HIF/HIF Prolyl-Hydroxylase Isoforms :

All Caspase Isoforms :

All Interleukin Related Isoforms :

In Vitro

Protectin D1 (1 μM, 24h) exerts cardioprotective effects in primary neonatal rat cardiomyocytes by upregulating miRNA-210 expression and activating the PI3K/AKT signaling pathway^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Real Time qPCR^[3]

Cell Line:

Primary neonatal rat cardiomyocytes
Concentration:

1 μM
Incubation Time:

24h
Result:

Upregulated miRNA-210 expression.

Western Blot Analysis^[3]

Cell Line:

Primary neonatal rat cardiomyocytes
Concentration:

1 μM
Incubation Time:

24h
Result:

Increased p-AKT and HIF-1α protein levels.

In Vivo

Protectin D1 (0.5, 1 μM/kg/day, i.p., once daily for 3 days) improves cardiac function and reduced infarct size in I/R-induced AMI rat model by inhibiting oxidative stress and inflammation^[3].
Protectin D1 (0.08 mg/kg, i.p., single dose) exerts anti-inflammatory effects in CLP-induced sepsis rat model by reducing ROS concentration, inhibiting NALP3 inflammasome activation, and decreasing pro-inflammatory cytokine levels^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

I/R-induced acute myocardial infarction (AMI) rat model, male Sprague Dawley rats (8 weeks old)^[3]
Dosage:

0.5 μM/kg, 1 μM/kg
Administration:

Intraperitoneal injection (i.p.), once daily for 3 days before surgery
Result:

Significantly reduced I/R-induced heart rate (HR), improved left ventricular ejection fraction (EF%) and fractional shortening (FS%), and reduced myocardial infarction area.

Animal Model:

Cecal ligation and puncture (CLP)-induced sepsis Wistar rat model (8 weeks old, 200 g)^[4]
Dosage:

0.08 mg/kg
Administration:

Intraperitoneal injection (i.p.), single dose (12 hours before surgery)
Result:

Significantly reduced ROS concentration in hepatocytes of CLP-induced rats, decreased NALP3, ASC, and Caspase-1 expression levels, and lowered serum IL-18 and IL-1β concentrations.

Chemical Information

CAS No. 660430-03-5
Appearance Liquid
Molecular Weight 360.49
Formula C₂₂H₃₂O₄
Color Colorless to light yellow
SMILES

CC/C=C\C[C@H](O)/C=C\C=C\C=C\[C@H](O)C/C=C\C/C=C\CCC(O)=O
Synonyms

Neuroprotectin D1; NPD1
Structure Classification
- Ketones, Aldehydes, Acids
Initial Source
- Endogenous metabolite
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Solution, -20°C, 2 years

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (268 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Tungen JE, et al. Synthesis of protectin D1 analogs: novel pro-resolution and radiotracer agents. Org Biomol Chem. 2018;16(36):6818-6823. [Content Brief]

[2]. Serhan CN, et al. Anti-inflammatory actions of neuroprotectin D1/protectin D1 and its natural stereoisomers: assignments of dihydroxy-containing docosatrienes [published correction appears in J Immunol. 2006 Mar 15;176(6):3843]. J Immunol. 2006;176(3):1848-1859. [Content Brief]

[3]. Zhang P, et al. Protectin D1 Alleviates Myocardial Ischemia/Reperfusion Injury by Regulating PI3K/AKT Signaling Pathway. J Cardiovasc Transl Res. 2024 Apr;17(2):376-387. [Content Brief]

Calculators

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

100 mg

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Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Purity & Documentation
References

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Protectin D1

All Endogenous Metabolite Isoforms

All PI3K Isoforms

All Akt Isoforms

All HIF/HIF Prolyl-Hydroxylase Isoforms

All Caspase Isoforms

All Interleukin Related Isoforms

Biological Activity

Chemical Information

Purity & Documentation

References

Calculators

Help & FAQs

Protectin D1 Related Classifications

HY-130413 Related Classifications