Physalin A
Based on 1 publication(s) in Google Scholar
Physalin A is a biologically active withanolide. Physalin A shows anti-inflammatory, antifibrotic and ameliorative effects on autophagy in models of disc degeneration. Physalin A has antitumor activity and can induce apoptosis, ROS production and G2/M phase cell cycle arrest. Besides. Physalin A can significantly increase the activity of quinone reductase and increase the expression of detoxifying enzymesc.
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- Reinheit: 99.63%
- CAS. Nr.: 23027-91-0
- Formel: C28H30O10
- Molecular Weight:526.53
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Speicherung:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Biologische Aktivität
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Chemical Information
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Physalin A
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Biologische Aktivität
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Cell Line
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Type | Value | Description | References |
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| HeLa | IC50 |
>50 μM
Compound: PA-2
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Inhibition of TNFalpha-induced IkappaBalpha (unknown origin) phosphorylation expressed in human HeLa cells pretreated 30 mins before TNFalpha addition measured up to 120 mins by ELISA in presence of proteasome inhibitor MG-132
Inhibition of TNFalpha-induced IkappaBalpha (unknown origin) phosphorylation expressed in human HeLa cells pretreated 30 mins before TNFalpha addition measured up to 120 mins by ELISA in presence of proteasome inhibitor MG-132
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[PMID: 24900739] |
| HeLa | IC50 |
22.7 μM
Compound: PA-2
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Inhibition of TNFalpha-induced NF-kappaB activation (unknown origin) expressed in human HeLa cells pretreated 30 mins before TNFalpha addition after 7 hrs by luciferase reporter gene assay
Inhibition of TNFalpha-induced NF-kappaB activation (unknown origin) expressed in human HeLa cells pretreated 30 mins before TNFalpha addition after 7 hrs by luciferase reporter gene assay
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[PMID: 24900739] |
| HT-1080 | IC50 |
10.7 μM
Compound: 1
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Cytotoxicity against human HT1080 cells assessed as growth inhibition after 24 hrs by MTT assay
Cytotoxicity against human HT1080 cells assessed as growth inhibition after 24 hrs by MTT assay
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[PMID: 23647462] |
| RAW264.7 | IC50 |
2.57 μM
Compound: 8
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Inhibition of LPS-induced nitric oxide production in mouse RAW264.7 cells assessed as nitrite formation
Inhibition of LPS-induced nitric oxide production in mouse RAW264.7 cells assessed as nitrite formation
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[PMID: 18348534] |
Physalin A (28.4 μM; 0-24 h) induces ROS production, G2/M phase cell cycle arrest and triggeres phosphorylation of P38 and ERK in A549 cells[2].
Physalin A (0-15 μM; 24 h) inhibits the cell viability and induces cell apoptosis in human non-small cell lung cancer[3].
Physalin A (20 μM; 48 h) improves TGF-β1-induced fibrosis in NP cells[4].
Physalin A (20 μM; 48 h) reduces inflammation by inhibiting NF-κB and MAPK signaling pathways and enhances autophagy by blocking PI3K/AKT/mTOR signaling pathways in IL-1β-treated NP cells[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:A549 cells
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Concentration:28.4 μM
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Incubation Time:6, 12 and 24 h
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Result:Increased the phosphorylation levels P38 and ERK in a time-dependent manner.
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Cell Line:H292, H358 and H1975 cells
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Concentration:0, 2.5, 5, 10 and 15 μM
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Incubation Time:24 h
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Result:Increased the levels of apoptotic markers, such as cleaved poly(ADP-ribose) polymerase (PARP), cleaved caspase-3 and cleaved caspase-9.
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Cell Line:NP cells were harvested from the inner tissue of tail disc of SpragueDawley rats
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Concentration:20 μM
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Incubation Time:48 h
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Result:Reduced the phosphorylation levels of IKKα/β, IκBα and P65.
Reduced the phosphorylation levels of JNK, ERK and P38.
Reduced the phosphorylation levels of PI3K. AKT and mTOR.
Physalin A (2 μL 20 μM; Injection of nucleus pulposus of the disc; Single dose) has a protective effect in a rat model of disc degeneration[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Male BALB/c mice[3]
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Dosage:40 and 80 mg/kg
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Administration:Intraperitoneal injection (i.p.); 10 days
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Result:Significantly reduced the mean tumor volume and tumor weight, had the least effect on the weight of mice.
Decreased Tyr705-p-STAT3 levels, and increased caspase-3 activation.
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Animal Model:Male Sprague-Dawley rats aged 6-8 weeks old (220±20 g) with intervertebral disc degeneration[4]
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Dosage:2 μL 20 μM
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Administration:Injection of nucleus pulposus of the disc; Single dose
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Result:Alleviated disc degradation.
Reduced inflammation and fibrosis.
Chemical Information
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CAS. Nr. 23027-91-0
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Appearance Solid
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Molecular Weight 526.53
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Formel C28H30O10
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Color White to off-white
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SMILES
C[C@@]([C@@](OC1=O)([H])C2)(OC3=O)[C@@]([C@@]3(CC[C@@]([C@]45C)([H])[C@@]6([H])[C@@H](C=C4CC=CC5=O)O)O)(O[C@@]76O)[C@](C7=O)([H])[C@]2(C1=C)C
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Structure Classification
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (1)
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Journal Impact Factor
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Most Recent
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Immunopharmacol Immunotoxicol
2024 Nov 3:1-12. PMID: 39491800
Reinheit & Dokumentation
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Data Sheet (287 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Verweise
[1]. Shin JM, et al. Physalin A regulates the Nrf2 pathway through ERK and p38 for induction of detoxifying enzymes. BMC Complement Altern Med. 2019 May 9;19(1):101. [Content Brief]
[2]. Kang N, et al. Physalin A induces G2/M phase cell cycle arrest in human non-small cell lung cancer cells: involvement of the p38 MAPK/ROS pathway. Mol Cell Biochem. 2016 Apr;415(1-2):145-55. [Content Brief]
[3]. Zhu F, et al. Physalin A exerts anti-tumor activity in non-small cell lung cancer cell lines by suppressing JAK/STAT3 signaling. Oncotarget. 2016 Feb 23;7(8):9462-76. [Content Brief]
[4]. Lu R, et al. Physalin A alleviates intervertebral disc degeneration via anti-inflammatory and anti-fibrotic effects. J Orthop Translat. 2023 Jan 25;39:74-87. [Content Brief]
Calculators
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