Ironomycin
Based on 1 Customer Validation
Ironomycin is a derivative of Salinomycin (HY-15597). Ironomycin exhibits selective inhibitory activity against mantle cell lymphoma (MCL) cells. Ironomycin blocks the cell cycle and induces apoptosis and ferroptosis. Ironomycin induces double-strand DNA breaks and activates the unfolded protein response (UPR), particularly the IRE1α signaling pathway accumulation. The combination of Ironomycin with Ibrutinib (HY-10997) shows a synergistic effect. Ironomycin can be used for the study of MCL.
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- Pureza : 99.85%
- No. CAS: 1884647-72-6
- Fòrmula: C45H73NO10
- Peso molecular:788.06
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Almacenamiento:
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Protocolo
- Pureza y Documentación
- Referencias
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Help & FAQs
Ver todos los productos específicos de isoformas DNA/RNA Synthesis
MoreVer todos los productos específicos de isoformas Caspase
More
Actividad biológica
Descripciòn
IC50 & Target
[2]|
CDK4/cyclin D2 |
Cdk4/cyclin D1 |
Caspase 3 |
Caspase 8 |
Caspase 9 |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HL-60 | GI50 |
10.5 μM
Compound: 16
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Cytotoxicity against human HL60 cells assessed as growth inhibition after 24 hrs by resazurin dye based assay
Cytotoxicity against human HL60 cells assessed as growth inhibition after 24 hrs by resazurin dye based assay
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[PMID: 30986574] |
Chemical Information
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No. CAS 1884647-72-6
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Appearance Solid
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Peso molecular 788.06
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Fòrmula C45H73NO10
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Color White to off-white
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SMILES
C#CCN[C@H]1[C@@]2(O[C@@](CC2)([C@]3([H])O[C@H]([C@](O)(CC3)CC)C)C)O[C@]4(C=C1)O[C@]([C@H](C[C@H]4C)C)([H])[C@@H](CC)C([C@@H](C)[C@@H](O)[C@@H]([C@]5([H])O[C@@](CC[C@@H]5C)([H])[C@@H](CC)C(O)=O)C)=O
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Solvente y solubilidad
In Vitro:
DMSO : 50 mg/mL (63.45 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (3.17 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (278 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Antoszczak M, et al. Rapid Access to Ironomycin Derivatives by Click Chemistry. ACS Org Inorg Au. 2022 Jun 1;2(3):222-228. [Content Brief]
[2]. Ovejero S, et al. Ironomycin induces mantle cell lymphoma cell death by targeting iron metabolism addiction. Theranostics. 2025 Feb 3;15(7):2834-2851. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.2689 mL | 6.3447 mL | 12.6894 mL | 31.7235 mL |
| 5 mM | 0.2538 mL | 1.2689 mL | 2.5379 mL | 6.3447 mL | |
| 10 mM | 0.1269 mL | 0.6345 mL | 1.2689 mL | 3.1723 mL | |
| 15 mM | 0.0846 mL | 0.4230 mL | 0.8460 mL | 2.1149 mL | |
| 20 mM | 0.0634 mL | 0.3172 mL | 0.6345 mL | 1.5862 mL | |
| 25 mM | 0.0508 mL | 0.2538 mL | 0.5076 mL | 1.2689 mL | |
| 30 mM | 0.0423 mL | 0.2115 mL | 0.4230 mL | 1.0574 mL | |
| 40 mM | 0.0317 mL | 0.1586 mL | 0.3172 mL | 0.7931 mL | |
| 50 mM | 0.0254 mL | 0.1269 mL | 0.2538 mL | 0.6345 mL | |
| 60 mM | 0.0211 mL | 0.1057 mL | 0.2115 mL | 0.5287 mL |
Ironomycin Related Classifications
HY-150280 Related Classifications
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