AG01

MDA-MB-231, HS578-T cells

0, 25, 50, 100, 200, 300 µg/mL

48, 72 h

Inhibited cells proliferation, resulted in a dose-dependent inhibition of MDA-MB-231 and HS578-T cells proliferation by 44% and 51% at 300 µg/mL compared with control.

MDA-MB-231, HS578-T cells

25, 50, 100 µg/mL

5 h

Inhibited cells migration in a dose-dependent, with the highest reduction of 78.2%, 77.5% at 100 µg/mL for MDA-MB-231, HS578-T cells.

MDA-MB-231, HS578-T cells

100 µg/mL

24, 48, 72 h

Decreased the phosphorylation status of Akt (14.8%), Src (23.6%), and Erk1/2 (20.3%) at 24 h and decreased phosphorylation of Akt (63%), Src (55.4%), and Erk1/2 (55.2%) at 72 h compared to control, decreased the ratio of phosphorylated protein to its total corresponding protein expression for Akt (71.9%), Src (49.8%), and Erk1/2 (62%) at 72 h in MDA-MB-231 cells.
Decreased the phosphorylation status of Akt (44.5%), Src (31.5%), and ERK1/2 (35.5%) at 24 h while decreased FAK phosphorylation (44.5%) at 48 h, decreased the ratio of phosphorylated protein to its total corresponding protein expression for p-Src/Src (44.3%), p-Akt/Akt (47.3%), and p-ERK1/2/ERK (46%) at 48 h in HS578-T cells.
Reduced c-MET expression of 59.7%.
Reduced SNAIL expression of 23.3% and 89%, ICAM-1 of 93.8% and 33.8%, AXL of 13.2% and 33.4% in MDA-MB-231 and HS578-T cells, respectively, reduced endogenous progranulin level in time-dependent reduction with MDA-MB-231 showing 37.7% reduction by 72 h and HS578-T showing almost a 100% reduction by 48 h.
Reduced cell adhesion and migratory protein-fbronectin in time-dependent decrease of 25.4% at 72 h as compared to its control lysate in MDA-MB-231 cells.