Y-27632

Y-27632 purchased from MedChemExpress. Usage Cited in: Nature. 2022 Jan;601(7894):600-605. [Abstract]

Upon exposure to lysophosphatidic acid (LPA) (a Hippo pathway inhibitor), A83-01 (an inhibitor of TGFβ family receptors) and PD0325901 (an ERK inhibitor) in a chemically defined medium containing the STAT activator leukaemia inhibitory factor (LIF) and Y-27632 (a ROCK inhibitor) (10 µM, 7 days),blastocyst-like structures formed efficiently from naive PSCs.

Y-27632 purchased from MedChemExpress. Usage Cited in: Science. 2025 Jun 5;388(6751):eadu9375. [Abstract]

On day 0, hPSCs were seeded onto new Matrigel- coated 6-well plates in StemMACS iPS-Brew XF medium + 10 μM Y-27632. On days 1 to 4, NC induction medium was added and changed daily. On days 5 and 6, NC induction medium + 1 μM retinoic acid was added daily. On day 7, cells were dissociated and split 1:6 in NC maintenance medium + 1 μM retinoic acid + 10 μM Y-27632. On days 8 and 9, NC maintenance medium + 1 μM retinoic acid was refreshed daily. On day 10, cells were dissociated and split. From days 11 to 21, SMC induction medium was refreshed daily. On days 22 to 28, cells were maintained in Medium 231+ Smooth Muscle Growth Supplement. Confocal fluorescence imaging and immunostaining confirms the differentiation of hESC-3R into CMs (coexpression of troponin-T with TNNT2-GFP), ECs (coexpression of VE-cadherin with CDH5-mOrange), and SMCs (coexpression of SM22α with TAGLN-CFP).

Y-27632 purchased from MedChemExpress. Usage Cited in: Cancer Cell. 2024 Jun 10;42(6):1086-1105.e13. [Abstract]

Y-27632 (GBCs; 10 uM). Representative H&E of primary RPM tumor (top) vs GBC-derived RPM allograft (bottom) 。All scale bars, 50 μm. 

Y-27632 purchased from MedChemExpress. Usage Cited in: Cell. 2024 Nov 14;187(23):6566-6583.e22. [Abstract]

Naive hPSCs were seeded into 300 or 400-micrometer hydrogel microwells in N2B27 with Y-27632 (10 μM). The next day, the medium was changed to PALLY medium, and half of the medium was replaced with fresh PALLY daily. IF staining for the EPI (SOX2), HYPO (GATA4), and TE markers (GATA3) on control blastoids (day 4). TE, trophectoderm; EPI, epiblast; HYPO, hypoblast.

Y-27632 purchased from MedChemExpress. Usage Cited in: Cell Stem Cell. 2022 Oct 6;29(10):1475-1490.e6. [Abstract]

The iPSCs were differentiated into lung airway organoids under s series of reagents which contains 10 μM Y-27632. The differentiation was confirmed by immunostaining with antibodies directed against airway markers, including E-Cadherin, FOXJ1 and acetylated α-tubulin, and MUC5AC

Y-27632 purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2020 Jun 17;7(15):1903583. [Abstract]

Fluorescence imaging of LNCaP cells treated with the F-actin inhibitor cytoskeleton B (CB), microtubule inhibitor nocodazole (NO), FAK inhibitor PF573288 (PF), ROCK inhibitor Y-27632 (Y), and myosin II (Myo-II) inhibitor (-)-blebbistatin ((-)Bl). After the cells were cultured on different substrates for 3 days, inhibitors were added and incubated for 24 h. (green, F-actin; scale bar, 25 µm).

Y-27632 purchased from MedChemExpress. Usage Cited in: J Exp Clin Cancer Res. 2018 Jun 28;37(1):128. [Abstract]

MHCC-97H and Huh-7 cells co-transfected with control or supervillin-specific siRNA and a RhoA(WT), RhoA(V14), or RhoA(N19) plasmids for 48 h are treated with PBS or Y27632 2HCl (10 μM) for 16 h during hypoxia, and then assayed for phosphorylated p38 and ERK by immunoblotting. β-actin is used as the loading control

Y-27632 purchased from MedChemExpress. Usage Cited in: Sci Adv. 2021 Sep 17;7(38):eabb5933. [Abstract]

Y27632 dihydrochloride (5 mg/kg/day; i.p.) increases both IFN-I and IFN-III production in VSV-infected intestine organoids.