Cat. No.: HY-N0534

Vitexin-2"-O-rhamnoside

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Vitexin-2"-O-rhamnoside is an orally active flavonoid glycoside. Vitexin-2"-O-rhamnoside inhibits Apoptosis, increases the phosphorylation levels of PI3K/Akt, inhibits caspase-3, SOD activity, and promotes cytokine (IL-2, IL-6, and IL-12) secretion. Vitexin-2"-O-rhamnoside strongly inhibits DNA synthesis in MCF-7 cells with an IC₅₀ of 17.5 μM. Vitexin-2"-O-rhamnoside enhances immune function and improves the absorption of active compounds. Vitexin-2"-O-rhamnoside has antioxidant activity. Vitexin-2"-O-rhamnoside is used in the study of cardiovascular disease and immune-related diseases.

For research use only. We do not sell to patients.

Purity: 99.78%
CAS No.: 64820-99-1
Formula: C₂₇H₃₀O₁₄
Molecular Weight:578.52

Storage:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Vitexin-2"-O-rhamnoside Chemical Structure

CAS No.: 64820-99-1

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target^[1]^[2]^[3]^[4]^[5]

IL-6

Caspase-3

IL-12

IL-2

All DNA/RNA Synthesis Isoforms :

All PI3K Isoforms :

All Akt Isoforms :

All Caspase Isoforms :

All Interleukin Related Isoforms :

In Vitro

Vitexin-2"-O-rhamnoside (62.5 μM; 24 h) can significantly alleviate the morphological deterioration, nuclear condensation of human adipose-derived stem cells induced by hydrogen peroxide, and reduce the percentage of apoptotic/necrotic cells and intracellular caspase-3 activity^[1].
Vitexin-2"-O-rhamnoside (15.6-125 μM; 1 h) can improve the viability of human umbilical vein endothelial cells ECV304^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

Human adipose-derived stem cells (hADSCs)
Concentration:

0 μM, 15.6 μM, 31.3 μM, 62.5 μM, 125 μM, 250 μM
Incubation Time:

24 h, 48 h, 72 h
Result:

Had no cytotoxic effect (up to 250 μM).
Significantly increased the cell viability of hADSCs to 106.93%, 124.24%, and 127.08% at 24 h, 48 h and 72 h, respectively (at 62.5 μM).

In Vivo

Vitexin-2"-O-rhamnoside (30 mg/kg; p.o. or i.v.) is mainly excreted as the prototype through biliary and renal routes in mice, with a significant first-pass effect after oral administration^[3].
Vitexin-2"-O-rhamnoside (50-200 mg/kg; i.g.; 14 days) improves immunosuppression, oxidative stress, and phosphorylation of PI3K/Akt signal pathway in Cyclophosphamide (HY-17420) treated mice^[4].
Vitexin-2"-O-rhamnoside (30 mg/kg; p.o.) shows a significantly increased oral bioavailability in rats when co-administered with Verapamil (HY-14275) or high-concentration Bile salts^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

SPF male Kunming strain mice (male, 20 g)^[3]
Dosage:

30 mg/kg
Administration:

Oral gavage (p.o.) or intravenous injection (i.v.)
Result:

Had a cumulative ratio unchanged in urine and feces of 4.13% and 38.89% after oral administration and 12.83% and 24.65% after intravenous administration, respectively.
Had a total recovery of 37.48% in feces (12.83% in urine and 24.65% in feces) after intravenous administration and 43.02% in feces (4.13% in urine and 38.89% in feces) after oral administration.

Animal Model:

Male BALB/c mice (male, 18-22 g, 6-8 weeks old) + immunosuppressive model induced by Cyclophosphamide^[4]
Dosage:

50 mg/kg, 100 mg/kg, 200 mg/kg
Administration:

Intragastric administration, 14 days
Result:

Improved weight loss symptoms in immunosuppressed mice.
Increased the thymus and spleen indices, restored the damage of CY to the thymus and spleen, alleviated the decrease in RBC, WBC, HGB, and PLT levels.
Promoted the proliferation of spleen lymphocytes, B cells, and T cells.
Enhanced NK and CTL activities, promoted the secretion and mRNA expression of cytokines IFN-γ, IL-2, IL-6, and IL-12.
Increased the activities of GSH-Px, CAT, SOD, and T-AOC, and decreased the level of MDA.
Increased the phosphorylation levels of PI3K and Akt in a dose-dependent manner.

Chemical Information

CAS No. 64820-99-1
Appearance Solid
Molecular Weight 578.52
Formula C₂₇H₃₀O₁₄
Color White to yellow
SMILES

OC1=CC(O)=C2C(OC(C3=CC=C(O)C=C3)=CC2=O)=C1[C@H](O[C@H](CO)[C@@H](O)[C@@H]4O)[C@@H]4O[C@@](O[C@@H](C)[C@H](O)[C@H]5O)([H])[C@@H]5O
Structure Classification
- Flavonoids
- Flavones
- Phenols
- Polyphenols
Initial Source
- Plants
- other families
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (172.85 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.7285 mL

8.6427 mL

17.2855 mL

5 mM

0.3457 mL

1.7285 mL

3.4571 mL

10 mM

0.1729 mL

0.8643 mL

1.7285 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (4.32 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (4.32 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (284 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Wei W, et al. Effects of vitexin-2"-O-rhamnoside and vitexin-4"-O-glucoside on growth and oxidative stress-induced cell apoptosis of human adipose-derived stem cells. J Pharm Pharmacol. 2014 Jul;66(7):988-97. [Content Brief]

[2]. Ying X, et al. LC determination of malondialdehyde concentrations in the human umbilical vein endothelial cell culture medium: application to the antioxidant effect of Vitexin-2 ″-O-rhamnoside. Chromatographia, 2008, 67: 679-686.

[3]. Cai S, et al. Comparative study on the excretion of vitexin-4''-O-glucoside in mice after oral and intravenous administration by using HPLC. Biomed Chromatogr. 2013 Nov;27(11):1375-9. [Content Brief]

[4]. Wang Y, et al. Vitexin-2-O-rhamnoside improves immunosuppression, oxidative stress, and phosphorylation of PI3K/Akt signal pathway in cyclophosphamide treated mice. Eur J Pharmacol. 2022 Jun 15;925:174999. [Content Brief]

[5]. Lu D, et al. Bioavailability of vitexin-2”-O-rhamnoside after oral co-administration with ketoconazole, verapamil and bile salts. Latin Am J Pharm, 2013, 32: 1218-23.

[6]. Ninfali P, et al. Characterization and biological activity of the main flavonoids from Swiss Chard (Beta vulgaris subspecies cycla). Phytomedicine. 2007 Feb;14(2-3):216-21. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.7285 mL	8.6427 mL	17.2855 mL	43.2137 mL
	5 mM	0.3457 mL	1.7285 mL	3.4571 mL	8.6427 mL
	10 mM	0.1729 mL	0.8643 mL	1.7285 mL	4.3214 mL
	15 mM	0.1152 mL	0.5762 mL	1.1524 mL	2.8809 mL
	20 mM	0.0864 mL	0.4321 mL	0.8643 mL	2.1607 mL
	25 mM	0.0691 mL	0.3457 mL	0.6914 mL	1.7285 mL
	30 mM	0.0576 mL	0.2881 mL	0.5762 mL	1.4405 mL
	40 mM	0.0432 mL	0.2161 mL	0.4321 mL	1.0803 mL
	50 mM	0.0346 mL	0.1729 mL	0.3457 mL	0.8643 mL
	60 mM	0.0288 mL	0.1440 mL	0.2881 mL	0.7202 mL
	80 mM	0.0216 mL	0.1080 mL	0.2161 mL	0.5402 mL
	100 mM	0.0173 mL	0.0864 mL	0.1729 mL	0.4321 mL

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Vitexin-2"-O-rhamnoside

Bioactive Compound Library Max

Bioactive Compound Library

Apoptosis Compound Library

Cell Cycle/DNA Damage Compound Library

Immunology/Inflammation Compound Library

Kinase Inhibitor Library

PI3K/Akt/mTOR Compound Library

Stem Cell Signaling Compound Library

Natural Product Library

Anti-Cancer Compound Library

Autophagy Compound Library

Anti-Aging Compound Library

Antioxidant Compound Library

Differentiation Inducing Compound Library

Glycoside Compound Library

Oxygen Sensing Compound Library

Anti-Cardiovascular Disease Compound Library

Medicine Food Homology Compound Library

Phenols Library

Glycolysis Compound Library

Pyroptosis Compound Library

Cytoskeleton Compound Library

Orally Active Compound Library

Glutamine Metabolism Compound Library

Traditional Chinese Medicine Active Compound Library

FDA Approved & Pharmacopeial Drug Library

Flavonoids Library

Anti-Breast Cancer Compound Library

Anti-Lung Cancer Compound Library

Anti-Pancreatic Cancer Compound Library

Anti-Blood Cancer Compound Library

Anti-Cancer Metabolism Compound Library

Neurodegenerative Disease-related Compound Library

Anti-Obesity Compound Library

Angiogenesis-Related Compound Library

Glucose Metabolism Compound Library

Food-Sourced Compound Library

Anti-Liver Cancer Compound Library

Anti-Colorectal Cancer Compound Library

Anti-Cancer Natural Product Library

Antidepressant Compound Library

Anti-inflammatory Traditional Chinese Medicine Active Compound Library

Plant-Sourced Natural Product Library

Anti-Prostate Cancer Compound Library

Anti-Pulmonary Fibrosis Compound Library

Cancer Stem Cells Compound Library

Heterocyclic Compound Library

Pain-Related Compound Library

Protease Inhibitor Library

Membrane Protein-targeted Compound Library

Autoimmune Disease Compound Library

Cell Death Library

Serine/Threonine Kinase Inhibitor Library

Anti-Hematopathy Compound Library

Anti-Ovarian Cancer Compound Library

Multi-Target Compound Library

Radioprotector Library

MCE Bioactive Compound Library

Natural Product Library Plus

Natural Product and Natural Product-Like Compound Library

Anti-Gastric Cancer Compound Library

Anti-Fibrosis Compound Library

Anti-Brain Cancer Compound Library

PBCRBS Traditional Chinese Medicine Compound Library

Protein Kinase Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

Cell Proliferation Compound Library

High-Throughput Bioactive Compound Library

High-Throughput Natural Product Library

Anti-Rheumatic Arthritis Compound Library

Natural Product Diversity Scaffold Library

Lactylation Compound Library

Mass Spectrometry Natural Product Library

All DNA/RNA Synthesis Isoforms

All PI3K Isoforms

All Akt Isoforms

All Caspase Isoforms

All Interleukin Related Isoforms

Biological Activity