Cat. No.: HY-N2993

Polyporenic acid C

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Based on 1 Customer Validation

Polyporenic acid C is a lanostane-type triterpenoid. Polyporenic acid C can be isolated from Poria cocos. Polyporenic acid C causes the cleavage of caspase-8 and caspase-3, as well as the cleavage of PARP. Polyporenic acid C reduces the phosphorylation level of Akt (Ser473), increases the phosphorylation of PTEN and p53 (Ser15), and activates JNK. Polyporenic acid C induces Apoptosis. Polyporenic acid C shows anticancer activity against non-small cell lung cancer.

For research use only. We do not sell to patients.

Purity: 98.81%
CAS No.: 465-18-9
Formula: C₃₁H₄₆O₄
Molecular Weight:482.69

Storage:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Polyporenic acid C Chemical Structure

CAS No.: 465-18-9

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

IC₅₀ & Target^[1]

Caspase-8

Caspase-3

All Caspase Isoforms :

All PARP Isoforms :

All Akt Isoforms :

All JNK Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	19.8 μM Compound: 93	Cytotoxicity against human A549 cells Cytotoxicity against human A549 cells	[PMID: 23092389]
BMDM	IC₅₀	4.9 μM Compound: 3	Inhibition of RANKL-induced C57BL/6 mouse BMDM differentiation into osteoclasts after 4 days in presence of M-CSF by TRAP assay Inhibition of RANKL-induced C57BL/6 mouse BMDM differentiation into osteoclasts after 4 days in presence of M-CSF by TRAP assay	[PMID: 27266877]
CCRF-CEM	IC₅₀	25.5 μM Compound: 8	Antiproliferative activity against human CCRF-CEM cells after 72 hrs by MTT assay Antiproliferative activity against human CCRF-CEM cells after 72 hrs by MTT assay	[PMID: 27808511]
HL-60	IC₅₀	31.8 μM Compound: 8	Antiproliferative activity against human HL60 cells after 72 hrs by MTT assay Antiproliferative activity against human HL60 cells after 72 hrs by MTT assay	[PMID: 27808511]
K562	IC₅₀	26.3 μM Compound: 8	Antiproliferative activity against human K562 cells after 72 hrs by MTT assay Antiproliferative activity against human K562 cells after 72 hrs by MTT assay	[PMID: 27808511]
MOLT-4	IC₅₀	27.6 μM Compound: 8	Antiproliferative activity against human MOLT4 cells after 72 hrs by MTT assay Antiproliferative activity against human MOLT4 cells after 72 hrs by MTT assay	[PMID: 27808511]

In Vitro

Polyporenic acid C (0-200 μM; 24-72 h) inhibits the proliferation of human non-small cell lung cancer A549 cells in a dose- and time-dependent manner^[1].
Polyporenic acid C (60-100 μM; 24-48 h) increases the apoptosis rate of human non-small cell lung cancer A549 cells in a dose- and time-dependent manner^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

Human non-small cell lung cancer A549 cells
Concentration:

2 μM, 6 μM, 20 μM, 60 μM, 200 μM
Incubation Time:

24 h, 48 h, 72 h
Result:

Significantly inhibited cell viability (84% of the control group remained viable) (72 h, 6 μM).
Reduced the relative cell viability by more than 50% (72 h, 60 μM).
Inhibited cancer cell growth by more than 90% (72 h, 200 μM).

Western Blot Analysis^[1]

Cell Line:

Human non-small cell lung cancer A549 cells
Concentration:

60 μM
Incubation Time:

24 h (for caspase, Akt, PTEN, p53 detection); 3 h, 6 h, 24 h (for JNK detection)
Result:

Led to the cleavage of caspase-8 and caspase-3, and the cleavage of PARP (a substrate of caspase-3), but did not induce the cleavage of caspase-9.
Reduced the phosphorylation level of Akt (Ser473), increased the phosphorylation of PTEN and p53 (Ser15), and activated JNK.

Chemical Information

CAS No. 465-18-9
Appearance Solid
Molecular Weight 482.69
Formula C₃₁H₄₆O₄
Color White to off-white
SMILES

C[C@]12[C@@]([C@]([C@H](C(O)=O)CCC(C(C)C)=C)([H])[C@H](O)C1)(CC=C3C2=CC[C@](C4(C)C)([H])[C@@]3(CCC4=O)C)C
Structure Classification
- Terpenoids
- Triterpenes
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 16.67 mg/mL (34.54 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.0717 mL

10.3586 mL

20.7172 mL

5 mM

0.4143 mL

2.0717 mL

4.1434 mL

10 mM

0.2072 mL

1.0359 mL

2.0717 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

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In Vivo:

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 50% PEG300 50% Saline

Solubility: 5 mg/mL (10.36 mM); Suspended solution; Need ultrasonic

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (275 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Ling H, et al. Polyporenic acid C induces caspase-8-mediated apoptosis in human lung cancer A549 cells.Mol Carcinog. 2009 Jun;48(6):498-507. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.0717 mL	10.3586 mL	20.7172 mL	51.7931 mL
	5 mM	0.4143 mL	2.0717 mL	4.1434 mL	10.3586 mL
	10 mM	0.2072 mL	1.0359 mL	2.0717 mL	5.1793 mL
	15 mM	0.1381 mL	0.6906 mL	1.3811 mL	3.4529 mL
	20 mM	0.1036 mL	0.5179 mL	1.0359 mL	2.5897 mL
	25 mM	0.0829 mL	0.4143 mL	0.8287 mL	2.0717 mL
	30 mM	0.0691 mL	0.3453 mL	0.6906 mL	1.7264 mL

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Polyporenic acid C

Apoptosis Compound Library

Cell Cycle/DNA Damage Compound Library

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All Caspase Isoforms

All PARP Isoforms

All Akt Isoforms

All JNK Isoforms

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table