Art. -Nr.: HY-16942

Damulin B

1 Cited Publications

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Damulin B is a dammarane-type saponin found in Gynostemma pentaphyllum. Damulin B can inhibit cancer cell apoptosis, decrease mitochondrial membrane potential, inhibit ROS production and cause G0/G1 phase arrest. Damulin B can prevent Cisplatin (HY-17394)-induced acute kidney injury and induce hair growth. Damulin B shows anti-inflammation anti-diabetic and anti-obesity effect. Damulin B can be used for the researches of cancer, inflammation, metabolic disease, such as lung cancer, osteoarthritis and diabetes.

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Reinheit: 96.62%
CAS. Nr.: 1202868-75-4
Formel: C₄₂H₇₀O₁₃
Molecular Weight:783.00

Speicherung:

-20°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Damulin B Chemical Structure

CAS. Nr.: 1202868-75-4

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (1)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target^[1]

CDK4

CDK6

Bcl-2

MMP-2

MMP-9

IL-24

Alle Bcl-2 Family Isoform-spezifische Produkte anzeigen :

Alle CDK Isoform-spezifische Produkte anzeigen :

Alle MMP Isoform-spezifische Produkte anzeigen :

Alle Interleukin Related Isoform-spezifische Produkte anzeigen :

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	23.8 μg/mL Compound: Damulin B	Cytotoxicity against human A549 cells assessed as decrease in viable cells after 48 hrs by CCK-8 assay Cytotoxicity against human A549 cells assessed as decrease in viable cells after 48 hrs by CCK-8 assay	[PMID: 26922140]

In Vitro

Damulin B inhibits A549 and H1299 cells growth with IC₅₀ values of 21.9 and 21.7 μM^[1].
Damulin B (20-24 μM, 24 h) induces apoptosis and ROS production in A549 and H1299 cells^[1].
Damulin B (20-24 μM, 24 h) cause the loss of mitochondrial membrane potential in A549 and H1299 cells^[1].
Damulin B (20-24 μM, 24 h) reduces colony formation and inhibits migration in A549 and H1299 cells^[1].
Damulin B (20-24 μM, 24 h) induces G0/G1 phase arrest in A549 and H1299 cells^[1].
Damulin B (2.5-20 μM, 24 h) inhibits apoptosis in Cisplatin (HY-17394)-induced HEK293 cells^[2].
Damulin B (0-80 μM, 24 h) shows no effect on cells viability in SW1353 cells^[4].
Damulin B (10-80 μM, 1 h) inhibits IL-1β-induced NO and PGE2 production in SW1353 cells^[4].
Damulin B (3-12 μM) inhibit cartilage-degrading enzymes in IL-1β-induced SW1353 cells^[4].
Damulin B (1.2-12 μM) increases 2-deoxy-[3H]D-glucose uptake in L6 myotube cells^[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:

A549 and H1299 cells
Concentration:

20 and 24 μM
Incubation Time:

24 h
Result:

Reduced Bcl-2, CDK4, CDK6, cyclin D1, cyclin E1 MMP-2 and MMMP-9 levels.
Increased IL-24, Bax, Bid, tBid and P53 levels.

Cell Cycle Analysis^[1]

Cell Line:

A549 and H1299 cells
Concentration:

20 and 24 μM
Incubation Time:

24 h
Result:

Increased cells in G0/G1 phase and reduced cells in S phase and G2/M phase.

In Vivo

Damulin B (25 and 50 mg/kg, i.p., daily for 7 days) suppresses oxidativestress and maintaining AMPKα1 levels in Cisplatin (HY-17394)-induced acute kidney injury mice^[2].
Damulin B (0.9 mL/day, p.o.) induce hair growth via the Wnt/β-catenin pathway through AKT signaling in mice^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Cisplatin (HY-17394)-induced acute kidney injury mice models^[1]
Dosage:

25 and 50 mg/kg
Administration:

Intraperitoneally injection, daily for 7 days
Result:

Increased AMPKα1 levels.
Prevented weight loss.
Attenuated tubular damage.

Chemical Information

CAS. Nr. 1202868-75-4
Appearance Solid
Molecular Weight 783.00
Formel C₄₂H₇₀O₁₃
Color White to off-white
SMILES

O[C@@H]([C@@H]([C@@H](CO)O1)O)[C@@H](O[C@H]2[C@@H]([C@H]([C@@H]([C@@H](CO)O2)O)O)O)[C@@H]1O[C@H]3[C@H](O)C[C@]4(C)[C@@]5([H])C[C@@H](O)[C@@H]6[C@@H](C(CC/C=C(C)\C)=C)CC[C@@]6(C)[C@@](C)5CC[C@@]4([H])C3(C)C
Structure Classification
- Terpenoids
- Triterpenes
Initial Source
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

-20°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Publications (1)

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Journal Impact Factor

Int J Mol Sci

Inhibitory Effects of Heat-Processed Gynostemma pentaphyllum Extract (Actiponin®) and Its Components on Cartilage Breakdown in Osteoarthritis. [Abstract]

2025 Feb 18;26(4):1728. PMID: 40004191

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : ≥ 50 mg/mL (63.86 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.2771 mL

6.3857 mL

12.7714 mL

5 mM

0.2554 mL

1.2771 mL

2.5543 mL

10 mM

0.1277 mL

0.6386 mL

1.2771 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 1.25 mg/mL (1.60 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: 1.25 mg/mL (1.60 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 1.25 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 1.25 mg/mL (1.60 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (284 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Shao-Fang Xing, et al. Inhibitory Effect of Damulin B from Gynostemma pentaphyllum on Human Lung Cancer Cells. Planta Med. 2019 Mar;85(5):394-405. [Content Brief]

[2]. Song M, et al. A Hydrodistillate of Gynostemma pentaphyllum and Damulin B Prevent Cisplatin-Induced Nephrotoxicity In Vitro and In Vivo via Regulation of AMPKα1 Transcription. Nutrients. 2022 Nov 24;14(23):4997. [Content Brief]

[3]. Kovale L, et al. Gynostemma pentaphyllum Hydrodistillate and Its Major Component Damulin B Promote Hair Growth-Inducing Properties In Vivo and In Vitro via the Wnt/β-Catenin Pathway in Dermal Papilla Cells. Nutrients. 2024 Mar 28;16(7):985. [Content Brief]

[4]. Lee SA, et al. Inhibitory Effects of Heat-Processed Gynostemma pentaphyllum Extract (Actiponin®) and Its Components on Cartilage Breakdown in Osteoarthritis. Int J Mol Sci. 2025 Feb 18;26(4):1728. [Content Brief]

[5]. Xu S, et al. Metabolomics and molecular docking-directed anti-obesity study of the ethanol extract from Gynostemma pentaphyllum (Thunb.) Makino. J Ethnopharmacol. 2024 Nov 15;334:118577. [Content Brief]

[6]. Nguyen PH, et al. New dammarane-type glucosides as potential activators of AMP-activated protein kinase (AMPK) from Gynostemma pentaphyllum. Bioorg Med Chem. 2011 Nov 1;19(21):6254-60. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.2771 mL	6.3857 mL	12.7714 mL	31.9285 mL
	5 mM	0.2554 mL	1.2771 mL	2.5543 mL	6.3857 mL
	10 mM	0.1277 mL	0.6386 mL	1.2771 mL	3.1928 mL
	15 mM	0.0851 mL	0.4257 mL	0.8514 mL	2.1286 mL
	20 mM	0.0639 mL	0.3193 mL	0.6386 mL	1.5964 mL
	25 mM	0.0511 mL	0.2554 mL	0.5109 mL	1.2771 mL
	30 mM	0.0426 mL	0.2129 mL	0.4257 mL	1.0643 mL
	40 mM	0.0319 mL	0.1596 mL	0.3193 mL	0.7982 mL
	50 mM	0.0255 mL	0.1277 mL	0.2554 mL	0.6386 mL
	60 mM	0.0213 mL	0.1064 mL	0.2129 mL	0.5321 mL

Keywords

Damulin B

1 Cited Publications

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Damulin B

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Publications Citing Use of MedChemExpress (MCE) Damulin B

Alle Bcl-2 Family Isoform-spezifische Produkte anzeigen

Alle CDK Isoform-spezifische Produkte anzeigen

Alle MMP Isoform-spezifische Produkte anzeigen

Alle Interleukin Related Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (1)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Damulin B Related Classifications

HY-16942 Related Classifications