Cat. No.: HY-125911

Gossypin

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Gossypin is an orally active flavone isolated from Hibiscus vitifolius. Gossypin has antioxidant, antiinflammatory, anticancer, anticataract, antidiabetic, and hepatoprotective activities. Gossypin inhibits NF-κB and NF-κB-regulated gene expression. Gossypin inhibits AURKA and RSK2. Gossypin inhibits invasion and induces apoptosis. Gossypin can be used for gastric cancer study.

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Pureté: 98.29%
CAS No.: 652-78-8
Formule: C₂₁H₂₀O₁₃
Masse moléculaire:480.38

Stockage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Gossypin Chemical Structure

CAS No.: 652-78-8

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Activité biologique
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Solvant et solubilité
- In Vitro
- In Vivo
Pureté et documentation
Références
Help & FAQs
Classifications
Bioz

Activité biologique

Description

IC₅₀ & Target

IC50: NF-κB^[1]

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CRY1
CRY2

In Vitro

Gossypin (10-100 μM, 0.5-8 h and then treats with TNF for 30 minutes) inhibits NF-κB activation, leading to potentiation of apoptosis, suppression of invasion in KBM-5 cells^[1].
Gossypin (50 μM, 3-5 days) inhibits RANKL-induced osteoclastogenesis in RAW 264.7 and mouse (C57BL/6J) primary bone marrow cells^[1].
Gossypin (10-60 μM, 72h or 2 weeks) inhibits gastric cancer cell growth^[3].
Gossypin (20-40 μM, 48 h) significantly suppresses migration in gastric cancer cell ^[3].
Gossypin (5-60 μM, 1-24 h) inhibits AURKA and RSK2 in JB6 cells and HGC27 cells^[3].
Gossypin (40 μM, 48 h) induces G2/M phase cell cycle arrest^[3].
Gossypin (40-60 μM, 72 h) increases apoptosis of gastric cancer cells^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cycle Analysis^[3]

Cell Line:

HGC27 human gastric cancer cells, AGS human gastric cancer cells
Concentration:

20, 40, 60 μM (72 h), or 10, 20, 40 μM (2 weeks)
Incubation Time:

(40 μM) 48 h or 72 h or 2 weeks
Result:

Reduced S phase and induced G2 phase cell cycle arrest.
Reduced the expression of cyclin A2, cyclin B1, and phosphorylated CDC2 proteins.
Growth of gastric cancer cells was significantly inhibited in a dose dependent manner.

Apoptosis Analysis^[3]

Cell Line:

HGC27 human gastric cancer cells
Concentration:

40, 60 μM
Incubation Time:

72 h
Result:

Increased the number of suspended cells (dead), decreased the number of adherent cells (live).
Early and late apoptosis cells were strongly increased compared to control cells.
Increased expression of pro-apoptotic marker proteins (cleaved caspase 3, 7 and 9, cleaved PARP and cytochrome c), decreased expression of anti-apoptotic marker protein (BcL-xL).

Cell Migration Assay ^[3]

Cell Line:

HGC27 human gastric cancer cells, AGS human gastric cancer cells
Concentration:

20, 40 μM
Incubation Time:

48 h
Result:

Significantly suppressed migration.

Western Blot Analysis^[1]

Cell Line:

KBM-5 (chronic myeloid leukemia)
Concentration:

0.5, 1, 2, 4, 8 h, and then treated with TNF for 30 minutes; or 2 h then treated with TNF for 16 h; or Preincubated for 30 minutes before add TNF, H₂O₂, PMA, LPS, IL-1β, OA, CSC
Incubation Time:

2.5 h (2 hours alone and then treated with TNF for 30 minutes)
Result:

Blocked NF-κB activation induced by tumor necrosis factor (TNF), H₂O₂, Phorbol myristate acetate (PMA), Lipopolysaccharides (LPS) (HY-D1056), interleukin-1β (IL-1β), okadaic acid (OA), cigarette smoke condensate (CSC).
Suppressed TNF-induced NF-κB in a dose-dependent manner.
Suppressed TNF-induced NF-κB in a time-dependent manner.
Enhanced TNF-induced apoptosis.

Western Blot Analysis^[1]

Cell Line:

RAW 264.7 cells, Mouse (C57BL/6J) primary bone marrow cells
Concentration:

50 μM
Incubation Time:

5 days (RAW 264.7 cells) or 3 days (primary bone marrow cells)
Result:

Decreased RANKL-induced osteoclast differentiation in RAW 264.7 cells.
50 μM concentration was sufficient to reduce osteoclastogenesis by more than 65% in RAW 264.7 cells.
Under these conditions, RAW 264.7cells remained fully viable.
Substantially decreased RANKL-induced osteoclast differentiation in primary bone marrow cells.

In Vivo

Gossypin (10-20 mg/kg; p.o.; 30 days) attenuates Isoprenaline (ISO) (HY-108353)-induced ischemic/reperfusion (I/R) in rats via alteration of oxidative stress, inflammatory reaction and gut microbiota^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Sprague Dawley (SD) rats (200-225 g; male; 8-10 weeks)^[2]
Dosage:

10, 20 mg/kg
Administration:

Oral gavage (p.o.); 30 days
Result:

Reduced the infarct size induced by Isoprenaline (ISO) (HY-108353).
Showed the boosted level of LVESP, SW, ESPVR, DP, EDPVR and suppressed level of LVEDP.
Increased the bodyweight and suppressed the heart weight and heart/body weight ratio.
Suppressed the level of triglyceride (TG), total cholesterol (TC), low-density lipoprotein (LDL), very low-density lipoprotein (VLDL) and increased the level of high-density lipoprotein (HDL).
Suppressed the level of nitric oxide synthase (iNOS) and nitric oxide (NO).
Decreased the level of MMP-2 and MMP-9.
Suppressed the level of inflammatory cytokines (TNF-α, IL-1β, IL-6) and decreased the level of NF-κB in the serum and heart tissue.
Suppressed the apoptosis of muscle fibres with edema and necrosis.

Chemical Information

CAS No. 652-78-8
Appearance Solid
Masse moléculaire 480.38
Formule C₂₁H₂₀O₁₃
Color Light yellow to yellow
SMILES

O=C1C(O)=C(C2=CC=C(O)C(O)=C2)OC3=C(O[C@H]4[C@@H]([C@H]([C@@H]([C@@H](CO)O4)O)O)O)C(O)=CC(O)=C13
Structure Classification
- Flavonoids
- Flavonols
- Phenols
- Polyphenols
Initial Source
Livraison

Room temperature in continental US; may vary elsewhere.

Stockage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvant et solubilité

In Vitro:

DMSO : 200 mg/mL (416.34 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.0817 mL

10.4084 mL

20.8169 mL

5 mM

0.4163 mL

2.0817 mL

4.1634 mL

10 mM

0.2082 mL

1.0408 mL

2.0817 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Calculateur de molarité

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculateur de dilution

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (4.33 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (4.33 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (4.33 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Pureté et documentation

Select Batch:

Pureté: 99.98%

Assay: 99.98%

ee.: 99.98%

Fiche technique (286 KB)
SDS (393 KB)
- English - EN (393 KB)
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- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instruction de manipulation (2659 KB)

Références

[1]. Ajaikumar B Kunnumakkara, et al. Gossypin, a pentahydroxy glucosyl flavone, inhibits the transforming growth factor beta-activated kinase-1-mediated NF-kappaB activation pathway, leading to potentiation of apoptosis, suppression of invasion, and abrogation of osteoclastogenesis. Blood. 2007 Jun 15;109(12):5112-21. [Content Brief]

[2]. Cheng G, et al. Cardioprotective Effect of Gossypin Against Myocardial Ischemic/Reperfusion in Rats via Alteration of Oxidative Stress, Inflammation and Gut Microbiota. J Inflamm Res. 2022 Mar 5;15:1637-1651. [Content Brief]

[3]. Wang L, et al. Gossypin inhibits gastric cancer growth by direct targeting of AURKA and RSK2. Phytother Res. 2019 Mar;33(3):640-650. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.0817 mL	10.4084 mL	20.8169 mL	52.0421 mL
	5 mM	0.4163 mL	2.0817 mL	4.1634 mL	10.4084 mL
	10 mM	0.2082 mL	1.0408 mL	2.0817 mL	5.2042 mL
	15 mM	0.1388 mL	0.6939 mL	1.3878 mL	3.4695 mL
	20 mM	0.1041 mL	0.5204 mL	1.0408 mL	2.6021 mL
	25 mM	0.0833 mL	0.4163 mL	0.8327 mL	2.0817 mL
	30 mM	0.0694 mL	0.3469 mL	0.6939 mL	1.7347 mL
	40 mM	0.0520 mL	0.2602 mL	0.5204 mL	1.3011 mL
	50 mM	0.0416 mL	0.2082 mL	0.4163 mL	1.0408 mL
	60 mM	0.0347 mL	0.1735 mL	0.3469 mL	0.8674 mL
	80 mM	0.0260 mL	0.1301 mL	0.2602 mL	0.6505 mL
	100 mM	0.0208 mL	0.1041 mL	0.2082 mL	0.5204 mL

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Gossypin

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Voir tous les produits spécifiques à Isoform NF-κB

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Activité biologique

Chemical Information